CN203999585U - A kind of enrichment adipose-derived stem cell automatic extracting device - Google Patents
A kind of enrichment adipose-derived stem cell automatic extracting device Download PDFInfo
- Publication number
- CN203999585U CN203999585U CN201420452008.7U CN201420452008U CN203999585U CN 203999585 U CN203999585 U CN 203999585U CN 201420452008 U CN201420452008 U CN 201420452008U CN 203999585 U CN203999585 U CN 203999585U
- Authority
- CN
- China
- Prior art keywords
- cup
- conduit
- liftable
- stem cell
- adipose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- External Artificial Organs (AREA)
Abstract
A kind of enrichment adipose-derived stem cell automatic extracting device belongs to medical instruments field.This apparatus main body is a cup that can seal, and liftable conduit and liposuction entry to mixed solution are installed on salable bowl cover, and liftable conduit is with hand-operated lifting, and cup is goed deep in one end, and one end connects negative pressure device by receiving flask; Cup body chassis is installed refuse relief outlet and motor, and motor rotarily drives cup body chassis rotation, for washing, centrifugal, acceleration precipitation.Liftable conduit is by manually controlling, and edge is airtight with lubricating fluid sealing, in moving range to a cup top, down to cup bottom.The steps such as that this device is integrated is centrifugal, extraction, precipitation, can shorten time of clinical extraction fat stem cell greatly, and total system closed operation, and controllability is strong, and Pollution risk greatly reduces.
Description
Technical field
The utility model belongs to medical instruments field, particularly a kind of enrichment adipose-derived stem cell automatic extracting device.
Background technology
Liposuction procedures belongs to the one in surgery Body contouring operation, principle be method by vacuum suction fat unnecessary a certain health position to sucking out, to reach the object of local rapid thin body.Liposuction procedures is common have following several: traditional Negative-pressure Liposuction, ultrasound-assisted liposuction art, water power lipsuction.
Ultrasound-assisted liposuction art is the ultrasonic wave that produces certain frequency by ultra-sonic generator; it is aimed to fat-reducing position; by ultrasonic concussion, adipocyte or fat particle are smashed; and then extrude or sucking-off; theory injured blood vessel nerve not on being, but practical application gets up to tell on slowly, still has the damage of nervus vasculairs; and adipocyte is destroyed seriously, be not suitable for and extract fat-derived stem cells and fat transfer.
Water power lipsuction is to impact subcutaneus adipose tissue by pulse hydraulic pressure, makes it depart from the constraint of reticular tissue, then application of negative pressure draws out, and this kind of method has to a certain degree and destroy fatty tissue, is not suitable for and extracts fat-derived stem cells and fat transfer.
Tradition Negative-pressure Liposuction, because of the appearance of swelling anaesthesia technology, be most widely used clinically at present, be expelled to belly by swelling liquid (every 1000ml physiological saline+50ml1% lignocaine+1ml (1:10000) suprarenin) in advance or shank is subcutaneous, then pass through to connect the liposuction conduit (the conventional blunt nosed side opening metal of diameter 3mm liposuction conduit) of negative pressure repeatedly in subcutaneous motion, subcutaneus adipose tissue, together with together sucking-off of inflation fluid, is deposited in container.
From above liposuction process, the clinical liposuction mixed solution obtaining, comprise following composition: the grease of generation, subcutaneous connective tissue, vascular stroma clastic constituents (SVF), red corpuscle etc. after adipocyte, adipocyte break, wherein useful composition comprises: adipocyte (for transplanting), SVF (comprising adipose-derived stem cell).
In transplant fat cell, add adipose-derived stem cell composition, can make the adipocyte of transplanting obtain higher survival rate, reach 70% left and right.(add in the absence of adipose-derived stem cell, the liquefaction that easily necroses of the adipocyte of transplanting, survival rate is about 20%～40%)
Therefore, extract clinically adipose-derived stem cell as needed, often by mixed solution through steps such as precipitation (approximately 1 hour), washing (application physiological saline), centrifugal (acceleration layerings), in this process, need be through repeatedly changing container, removing liquid waste, this has not only increased the risk of polluting, and complex steps, loses time.
Summary of the invention
For the problems referred to above, the utility model provides a kind of enrichment adipose-derived stem cell automatic extracting device, the steps such as this device integration is centrifugal, extraction, precipitation, can greatly shorten the time of clinical extraction fat stem cell, and total system closed operation, controllability is strong, and Pollution risk greatly reduces.
A kind of enrichment adipose-derived stem cell of the utility model automatic extracting device, this apparatus main body is a cup that can seal, and liftable conduit and liposuction entry to mixed solution are installed on salable bowl cover, and liftable conduit is with hand-operated lifting, cup is goed deep in one end, and one end connects negative pressure device by receiving flask; Cup body chassis is installed refuse relief outlet and motor, and motor rotarily drives cup body chassis rotation, for washing, centrifugal, acceleration precipitation.
Liftable conduit is by manually controlling, and edge is airtight with lubricating fluid sealing, in moving range to a cup top, down to cup bottom.
When use, be divided into following steps:
1 draw fatty mixed solution: liftable conduit rises to top, connect negative pressure, liposuction entry to mixed solution, by aseptic hose connection subcutaneous liposuction conduit, operates by patient, by subcutaneus adipose tissue together with inflation fluid together from liposuction entry to mixed solution embryo lead-in cup.
2 washings: when suction mixing liquid measure reaches cup 2/3, temporary close negative pressure, opens bottom motors, make bottom of cup rotation mix and blend, accelerate layering.Disable motor stratification, is divided into four layers after demixing in cup:
Bottom is SVF composition, is rich in fat-derived stem cells;
Layer second from the bottom is for washing physiological saline and local anaesthetics composition, useless, repeatedly sucking-off, washing;
The third from the bottom layer adipocyte for wash, can sucking-off, for transplanting;
Fourth from the last layer is oil layer, useless, can sucking-off.
Disable motor, decline liftable conduit, part sucking-off layer second from the bottom and the discarded composition of fourth from the last layer, inject from liposuction entry to mixed solution with physiological saline or Langerhans liquid, supplies after cup 2/3, can be as required, repeat above step 1～2 time.
3 obtain:
After washing, in mixed solution, the compositions such as local anaesthetics are diluted sucking-off, and mobile liftable conduit is changed and obtained bottle, obtains corresponding cellular constituent.After being mixed in proportion, the position that is injected in needs of patients and carries out fat transfer filling.
The utility model has the advantage of:
1. whole device is closed unit, washing, centrifugal, obtain a step and complete, greatly improved operation efficiency
2. by the rotation of bottom, can accelerate more efficiently the layering of liposuction mixed solution, precipitation,
3. liftable conduit, can be as required, and one or many extracts the benefit of required cellular constituent liftable conduit and can repeatedly wash exactly, extracts, and need not all change liquid, improves detersive efficiency and useful component and obtain efficiency.
4. the negative pressure connecting is obtained bottle, can, in the time that absorption is discarded also with effective cell composition, install respectively, reduces and pollutes generation, greatly raises the efficiency.
Brief description of the drawings
Fig. 1 is a kind of enrichment adipose-derived stem cell of the utility model automatic extracting device schematic diagram;
Wherein 1 is cup, and 1.1 is bowl cover, and 1.2 is cup body chassis, and 2 is liposuction entry to mixed solution, and 3 is liftable conduit, and 4 is motor, and 5 is refuse relief outlet, and 6 for obtaining bottle, and 7 is negative pressure device.
Embodiment
A kind of enrichment adipose-derived stem cell of the utility model automatic extracting device, this apparatus main body is a cup that can seal 1, liftable conduit 3 and liposuction entry to mixed solution 2 are installed on bowl cover 1.1, liftable conduit 3 can hand-operated lifting, cup 1 is goed deep in one end, and one end connects negative pressure device 7 by the aseptic disposable medical catheter of negative pressure by obtaining bottle 6; Cup body chassis 1.2 is installed refuse relief outlet 5 and motor 4, and motor 4 rotarily drives cup body chassis 1.2 and rotates, for washing, centrifugal, acceleration precipitation.
Liftable conduit 3 is by manually controlling, and edge is airtight with lubricating fluid sealing.
When use, open negative pressure device 7, subcutaneus adipose tissue is together imported cup 1 from entry to mixed solution 2 together with inflation fluid, manually make liposuction conduit 3 be positioned at liquid level top, open motor 4, make bottom of cup 1.2 rotate mix and blend and centrifugal, disable motor 4 stratification, automatic lifting is extracted conduit 3 to required liquid layer, opens negative pressure device 7, can be as required, one or many extracts required cellular constituent.
In cup, after demixing, bottom is SVF vascular stroma fragment composition, is rich in fat-derived stem cells ASCs; Layer second from the bottom is for washing physiological saline and local anaesthetics composition, useless, repeatedly sucking-off, washing; The third from the bottom layer adipocyte for wash, can sucking-off, for transplanting; Fourth from the last layer is oil layer, useless, can sucking-off.
Use this to use novel device to carry out clinical experiment 12 examples, average extraction time is 1h, (traditional method is 3h left and right), and occur without complication such as infection, transplant fat surviving rate Follow-up results half a year, on average survives 70% (after preoperative, injection, half a year Magnetic resonance imaging comparing result).
Claims (2)
1. an enrichment adipose-derived stem cell automatic extracting device, it is characterized in that this apparatus main body is a cup that can seal, liftable conduit and liposuction entry to mixed solution are installed on salable bowl cover, liftable conduit is with hand-operated lifting, cup is goed deep in one end, and one end connects negative pressure device by receiving flask; Cup body chassis is installed refuse relief outlet and motor, and motor rotarily drives cup body chassis rotation, for washing, centrifugal, acceleration precipitation.
2. a kind of enrichment adipose-derived stem cell automatic extracting device according to claim 1, is characterized in that described liftable conduit is by manually controlling, and edge is airtight with lubricating fluid sealing, in moving range to a cup top, down to cup bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420452008.7U CN203999585U (en) | 2014-08-12 | 2014-08-12 | A kind of enrichment adipose-derived stem cell automatic extracting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420452008.7U CN203999585U (en) | 2014-08-12 | 2014-08-12 | A kind of enrichment adipose-derived stem cell automatic extracting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203999585U true CN203999585U (en) | 2014-12-10 |
Family
ID=52039614
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420452008.7U Expired - Fee Related CN203999585U (en) | 2014-08-12 | 2014-08-12 | A kind of enrichment adipose-derived stem cell automatic extracting device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203999585U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520551A (en) * | 2016-10-20 | 2017-03-22 | 广东省心血管病研究所 | Stem cell extracting device |
| CN107475098A (en) * | 2017-08-15 | 2017-12-15 | 中国人民解放军第二军医大学第二附属医院 | Subpatellar fat pad is quickly homogenized packaging device and method under a kind of aseptic condition |
| CN109415675A (en) * | 2016-03-17 | 2019-03-01 | 辛诺瓦生命科学股份有限公司 | Use shock wave or the method for mechanical shock separation, dissociation and/or lytic cell |
| CN109925014A (en) * | 2019-04-03 | 2019-06-25 | 北京大学第三医院（北京大学第三临床医学院） | A kind of fat purifying plant |
| CN111269800A (en) * | 2020-02-28 | 2020-06-12 | 南京大学 | Device and method for extracting total DNA of matrix-attached microorganisms |
-
2014
- 2014-08-12 CN CN201420452008.7U patent/CN203999585U/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109415675A (en) * | 2016-03-17 | 2019-03-01 | 辛诺瓦生命科学股份有限公司 | Use shock wave or the method for mechanical shock separation, dissociation and/or lytic cell |
| CN106520551A (en) * | 2016-10-20 | 2017-03-22 | 广东省心血管病研究所 | Stem cell extracting device |
| CN107475098A (en) * | 2017-08-15 | 2017-12-15 | 中国人民解放军第二军医大学第二附属医院 | Subpatellar fat pad is quickly homogenized packaging device and method under a kind of aseptic condition |
| CN107475098B (en) * | 2017-08-15 | 2023-08-15 | 中国人民解放军第二军医大学第二附属医院 | Rapid homogenization split charging device and method for subpatellar fat pad under aseptic condition |
| CN109925014A (en) * | 2019-04-03 | 2019-06-25 | 北京大学第三医院（北京大学第三临床医学院） | A kind of fat purifying plant |
| CN109925014B (en) * | 2019-04-03 | 2024-03-22 | 北京阿拉布丁生物科技有限公司 | Fat purification device |
| CN111269800A (en) * | 2020-02-28 | 2020-06-12 | 南京大学 | Device and method for extracting total DNA of matrix-attached microorganisms |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203999585U (en) | A kind of enrichment adipose-derived stem cell automatic extracting device | |
| US11389616B2 (en) | Method for preparing tissue, particularly adipose tissue, for transplantation from lobular fat extracted by liposuction | |
| US8858518B2 (en) | Canister for autologous fat transfer | |
| JP2020093257A (en) | Fat tissue centrifugal separator and method of application thereof | |
| US20120016337A1 (en) | Collection canister apparatus for closed loop fat harvesting system and method of use thereof | |
| US20190125971A1 (en) | Small volume tissue processing devices | |
| CN107050516A (en) | A kind of fatty gel preparation method rich in fat stem cell and extracellular matrix | |
| WO2017195225A1 (en) | Method for extracting and separating stem cells derived from adipose tissue for aesthetic treatments | |
| US8936766B2 (en) | Device and method for delivering mechanically released cells from liposuction aspirates | |
| CN203724511U (en) | Thoracocentesis device operated by one person | |
| CN206607231U (en) | A kind of fat stem cell filter | |
| CN207452106U (en) | For the fat-derived primary stem cell collection piece-rate system of binary channels of human body liposuction | |
| CN206896543U (en) | Full-automatic autologous fat breast-enlarging apparatus | |
| CN206434894U (en) | The ultrasonic separation means of Chinese medicine | |
| CN212316092U (en) | Equipment for extracting adipose-derived stem cells by negative pressure separation | |
| CN204411094U (en) | A kind of ophthalmologic operation note inhales device | |
| CN204446947U (en) | The dual-purpose suction catheter of smoking imbibition under peritoneoscope | |
| CN213554153U (en) | Clinical thyroid gland postoperative drainage ware of department of general surgery | |
| CN213557478U (en) | Fat refiner and fat treatment station | |
| CN202213908U (en) | Nursing let flow collecting bottle | |
| KR20090129069A (en) | Method for isolating stem cell | |
| CN205308597U (en) | Fat splitting ware | |
| CN107475098B (en) | Rapid homogenization split charging device and method for subpatellar fat pad under aseptic condition | |
| CN202776507U (en) | Abortion suction unit collection bottle with filter screen | |
| CN204637184U (en) | A kind of syringe headkerchief filtered for fat |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141210 Termination date: 20150812 |
|
| EXPY | Termination of patent right or utility model |