CN212316092U - Equipment for extracting adipose-derived stem cells by negative pressure separation - Google Patents

Equipment for extracting adipose-derived stem cells by negative pressure separation Download PDF

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Publication number
CN212316092U
CN212316092U CN202020727016.3U CN202020727016U CN212316092U CN 212316092 U CN212316092 U CN 212316092U CN 202020727016 U CN202020727016 U CN 202020727016U CN 212316092 U CN212316092 U CN 212316092U
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negative pressure
separation
derived stem
extractor
stem cells
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龙笑
王晓军
陈洁
李竹君
崇煜明
张文超
王晨羽
俞楠泽
夏泽楠
黄久佐
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Peking Union Medical College Hospital Chinese Academy of Medical Sciences
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Peking Union Medical College Hospital Chinese Academy of Medical Sciences
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Abstract

The utility model provides an utilize negative pressure separation to extract equipment of fat source stem cell, include: the separation extractor is provided with a stirring device towards the inside of the upper cover, the upper cover is also provided with a to-be-treated mixed liquid inlet, the bottom of the separation extractor extends downwards to be provided with a guide pipe, the guide pipe is communicated with the inside of the separation extractor, and one end of the guide pipe is provided with a flow speed regulator; the collector comprises an upper-layer filtering part and a lower-layer collecting part which are movably connected, the other end of the conduit is inserted into the upper-layer filtering part, the bottom of the upper-layer filtering part is provided with a filter screen, and the upper part of the lower-layer collecting part is open; and the negative pressure device comprises a first pipeline, a negative pressure storage tank, a second pipeline and a suction device, the first pipeline enables the separation extractor to be communicated with the negative pressure storage tank, and the second pipeline enables the negative pressure storage tank to be communicated with the suction device. The utility model provides an equipment structure is simple, convenient operation, and is sealed effectual, and the filter screen can the filtering remain impurity.

Description

Equipment for extracting adipose-derived stem cells by negative pressure separation
Technical Field
The utility model relates to the technical field of medical equipment, especially, relate to an utilize negative pressure separation to extract equipment of fat source stem cell.
Background
The adipose-derived stem cell is an adult stem cell with the multipotentiality separated from adipose tissue, and shows great clinical application value in tissue repair and reconstruction application. Clinically, conventional negative pressure liposuction is generally used to extract adipose-derived stem cells. The negative pressure liposuction is characterized in that a principle of negative pressure suction is applied, a professional metal suction pipe is inserted into the subcutaneous part through a small skin incision, and the fat tissue accumulated on the part is sucked out while scraping. The adipose-derived stem cells can be separated and extracted from the aspirated adipose tissues by using an extraction device. In the prior art, a chinese utility model patent with an authorization publication number of CN203999585U discloses an automatic extraction device for enriched adipose-derived stem cells. The motor of the device is arranged on the cup body base, the motor can drive the whole cup body base to rotate when being started, and the structure can not ensure the sealing property of the bottom of the cup body. In addition, in this patent, useless composition and useful composition are collected through one and are acquireed the bottle for the extraction process is more loaded down with trivial details, and this patent does not set up filter equipment, can't realize better purification effect.
In view of the defects of the prior art, there is a need in the art for an adipose-derived stem cell isolation and extraction device which has better sealing performance, simple structure and convenient operation and can filter impurities in the adipose-derived stem cells.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem such as the impurity among the adipose-derived stem cell extraction element leakproofness of the adipose-derived stem cell among the prior art poor, complex operation, the unable adipose-derived stem cell that filters, the utility model provides an utilize the negative pressure separation to extract the equipment of adipose-derived stem cell.
The utility model discloses an utilize negative pressure separation to extract equipment of fat source stem cell, include:
the device comprises a separation extractor, wherein a stirring device is arranged towards the inside of an upper cover of the separation extractor, the upper cover is also provided with a to-be-treated mixed liquid inlet, the bottom of the separation extractor extends downwards to be provided with a guide pipe, the guide pipe is communicated with the inside of the separation extractor, and one end of the guide pipe, which is close to the bottom of the separation extractor, is provided with a flow speed regulator;
the collector comprises an upper-layer filtering part and a lower-layer collecting part which are movably connected, the other end of the conduit is inserted into the upper-layer filtering part, the bottom of the upper-layer filtering part is provided with a filter screen, and the upper part of the lower-layer collecting part is open; and
and a negative pressure device including a first pipe having one end inserted into the separation extractor from the upper cover and the other end inserted into the negative pressure storage tank, a negative pressure storage tank having one end inserted into the negative pressure storage tank and the other end connected to the suction device.
According to an embodiment of the present invention, the stirring device includes a motor, a rotation shaft, and a blade, which are connected in sequence.
According to the utility model discloses an embodiment, the upper filtering part with lower floor's collection portion is threaded connection.
According to an embodiment of the present invention, the aperture of the filter net is 500 μm to 800 μm.
The utility model discloses an utilize negative pressure separation to extract equipment of fat source stem cell has following beneficial effect: the utility model discloses an utilize negative pressure separation to extract equipment of fat source stem cell adopts overhead agitating unit, can guarantee that the separation extractor has good leakproofness. The collector is used for collecting useful components such as separated adipose-derived stem cells, and the useless components can be sucked into the negative pressure storage tank by the negative pressure device, so that the extraction process is simplified. The filter screen in the collector can filter impurities in the adipose-derived stem cells, thereby achieving the purpose of further purification.
Drawings
Fig. 1 is a schematic view of an apparatus for extracting adipose-derived stem cells using negative pressure separation according to an embodiment of the present invention.
Detailed Description
The present invention is described in more detail below with reference to examples, so that those skilled in the art can implement the present invention after studying the present specification. It should be understood that the detailed description and specific examples, while indicating the scope of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
It should be noted that all expressions using "first", "second", etc. in the embodiments of the present invention are used for distinguishing two or more entities with the same name but different names or different parameters, and it can be seen that "first", "second", etc. are only used for convenience of expression and should not be understood as limitations to the embodiments of the present invention, and the following embodiments do not describe this one by one.
Fig. 1 is a schematic view of an apparatus for extracting adipose-derived stem cells using negative pressure separation according to an embodiment of the present invention. Referring to fig. 1, the apparatus for extracting adipose-derived stem cells by negative pressure separation provided in this embodiment mainly includes a separation extractor 1, a collector 2, and a negative pressure device 3, where the separation extractor 1 is respectively communicated with the collector 2 and the negative pressure device 3. The separation and extraction unit 1 is used for centrifuging useful components and useless components in a mixed solution to be treated, which contains components such as fat cells, oil and fat, subcutaneous connective tissue, vascular stromal debris (SVF), red blood cells and the like. The collector 2 is used to collect the useful components (e.g. adipocytes, SVF) separated from the mixture to be treated. The negative pressure device 3 performs liposuction by using negative pressure and sucks out useless components from the mixed liquid to be treated obtained after liposuction.
As shown in fig. 1, an agitating device 12 is installed on an upper cover 11 of the separation extractor 1 toward the inside thereof, the upper cover 11 is further provided with a to-be-treated mixed liquid inlet 13, a conduit 14 extends downward from the bottom of the separation extractor 1, the conduit 14 is communicated with the inside of the separation extractor 1, and a flow rate regulator 15 is provided at one end of the conduit 14 near the bottom of the separation extractor 1. The stirring device 12 is an upper stirring device and is detachably mounted to the upper cover 11, facilitating disassembly for cleaning. The stirring device 12 comprises a motor, a rotating shaft and blades which are connected in sequence, wherein the motor is positioned outside the separation extractor 1, and the rotating shaft and the blades are positioned in the inner cavity of the separation extractor 1. The flow rate regulator 15 is used to open and close the passage formed by the duct 14 and to regulate the flow rate of the fluid in the passage.
The collector 2 comprises an upper filtering part 21 and a lower collecting part 22 which are movably connected, the other end of the conduit 14 is inserted into the upper filtering part 21, the bottom of the upper filtering part 21 is a filter screen 211, and the upper part of the lower collecting part 22 is open. Preferably, the upper filtering portion 21 and the lower collecting portion 22 are screwed. Preferably, the aperture of the filter screen 211 is 500 μm to 800 μm.
The negative pressure means 3 includes a first pipe 31, one end of which 31 is inserted into the separation extractor 1 from the upper cover 11 and the other end is inserted into the negative pressure storage tank 32, a second pipe 33, one end of which 33 is inserted into the negative pressure storage tank 32 and the other end is connected to the suction means 34, and a suction means 34. A first conduit 31 communicates the separation extractor 1 with the negative pressure storage tank 32, and a second conduit 33 communicates the negative pressure storage tank 32 with the suction device 34.
When using the utility model discloses an utilize negative pressure separation to extract equipment of fat source stem cell, at first, start negative pressure device 3 and inhale the mixed liquid of pending in separation extractor 1, then, close negative pressure device 3, start agitating unit 12, separation extractor 1 carries out centrifugal separation with useful component and useless component in the mixed liquid of pending, makes useful component and useless component layering stand, wherein, SVF is located the bottommost layer, consequently can be earlier through pipe 14 with SVF leading-in collector 2 spare. The unwanted components can be sucked into the negative pressure reservoir 32 by the negative pressure device 3. The SVF extraction process can be repeated as needed, during which the negative pressure storage tank 32 does not have to be replaced frequently, enabling the continuation of the negative pressure state of the apparatus to be ensured.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention and are not intended to limit the present invention in any way. It should be noted that any modification made by those skilled in the art without departing from the spirit and concept of the present invention falls within the protection scope of the present invention.

Claims (4)

1. An apparatus for extracting adipose-derived stem cells by negative pressure separation, comprising:
the device comprises a separation extractor, wherein a stirring device is arranged towards the inside of an upper cover of the separation extractor, the upper cover is also provided with a to-be-treated mixed liquid inlet, the bottom of the separation extractor extends downwards to be provided with a guide pipe, the guide pipe is communicated with the inside of the separation extractor, and one end of the guide pipe, which is close to the bottom of the separation extractor, is provided with a flow speed regulator;
the collector comprises an upper-layer filtering part and a lower-layer collecting part which are movably connected, the other end of the conduit is inserted into the upper-layer filtering part, the bottom of the upper-layer filtering part is provided with a filter screen, and the upper part of the lower-layer collecting part is open; and
and a negative pressure device including a first pipe having one end inserted into the separation extractor from the upper cover and the other end inserted into the negative pressure storage tank, a negative pressure storage tank having one end inserted into the negative pressure storage tank and the other end connected to the suction device.
2. The apparatus for extracting adipose-derived stem cells using negative pressure separation according to claim 1, wherein the agitating device comprises a motor, a rotating shaft, and blades connected in sequence.
3. The apparatus for extracting adipose-derived stem cells using negative pressure isolation according to claim 1, wherein the upper filtering part and the lower collecting part are screwed.
4. The apparatus for extracting adipose-derived stem cells using negative pressure isolation according to claim 1, wherein the mesh size of the strainer is 500 μm to 800 μm.
CN202020727016.3U 2020-05-06 2020-05-06 Equipment for extracting adipose-derived stem cells by negative pressure separation Active CN212316092U (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112852624A (en) * 2021-03-15 2021-05-28 上海交通大学医学院附属第九人民医院 Full-automatic adipose-derived stem cell extraction system
CN113174361A (en) * 2021-06-10 2021-07-27 南通市第一老年病医院(上海大学附属南通医院、南通市第六人民医院、南通市肺科医院) Pathological cell separation adsorption type extraction method
CN113621474A (en) * 2020-05-06 2021-11-09 中国医学科学院北京协和医院 Equipment for extracting adipose-derived stem cells by negative pressure separation

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113621474A (en) * 2020-05-06 2021-11-09 中国医学科学院北京协和医院 Equipment for extracting adipose-derived stem cells by negative pressure separation
CN112852624A (en) * 2021-03-15 2021-05-28 上海交通大学医学院附属第九人民医院 Full-automatic adipose-derived stem cell extraction system
CN113174361A (en) * 2021-06-10 2021-07-27 南通市第一老年病医院(上海大学附属南通医院、南通市第六人民医院、南通市肺科医院) Pathological cell separation adsorption type extraction method
CN113174361B (en) * 2021-06-10 2023-11-14 南通市第一老年病医院(上海大学附属南通医院、南通市第六人民医院、南通市肺科医院) Pathological cell separation adsorption type extraction method

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