CN203981682U - Epidemic meningitis A group, C group, Y group, the antibody combined test card of W135 group IgG - Google Patents
Epidemic meningitis A group, C group, Y group, the antibody combined test card of W135 group IgG Download PDFInfo
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- CN203981682U CN203981682U CN201420181814.5U CN201420181814U CN203981682U CN 203981682 U CN203981682 U CN 203981682U CN 201420181814 U CN201420181814 U CN 201420181814U CN 203981682 U CN203981682 U CN 203981682U
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Abstract
The open epidemic meningitis A group of the utility model, C group, Y group, the antibody combined test card of W135 group IgG, comprise liner (7), sample pad (1), fiberglass packing (2), chromatographic film (5) and absorption pad (6); Described sample pad (1), fiberglass packing (2), chromatographic film (5) and absorption pad (6) join with horizontal direction order and are fixed on described liner (7); And on described fiberglass packing (2), be coated with the colored latex particle of tape label; The first detection line (31), the second detection line (32), the 3rd detection line (33), the 4th detection line (34) and control line (4) are set in described chromatographic film (5).This test card gets final product joint-detection epidemic meningitis A group IgG antibody, epidemic meningitis C group IgG antibody, epidemic meningitis Y group IgG antibody, epidemic meningitis W135 group IgG antibody by single job process, thereby has simplified operating process, has realized the object of fast detecting.
Description
Technical field
The utility model belongs to technical field of immunoassay, particularly epidemic meningitis A group, C group, Y group, the antibody combined test card of W135 group IgG.
Background technology
Meningococal meningitis is the meningitis (abbreviation epidemic meningitis) being caused by meningococcus, is Acute respiratory infectious disease.Main clinical manifestation is petechia, ecchymosis and the meningismuses such as heating, headache, vomiting, skin and mucosa.Severe one can have septic shock and meningoencephalitis.This sick morbidity is anxious, and case fatality rate is high, and can occur in each age group, and following children's morbidity in 15 years old accounts for more than 75%.For effectively controlling the generation of meningococal meningitis, should shift to an earlier date prophylactic vaccination.
On market, have A group, C group, Y group, W135 group's polysaccharide combined vaccine now.For the detection after this combined vaccine, conventionally need to buy different kits and carry out respectively the detection of monospecific antibody, complicated operation, length consuming time, expends large.Although detect separately after A group IgG antibody, C group IgG antibody, Y group IgG antibody, W135 group IgG antibody, more comprehensively analyze testing result diagnostic result be there is no to impact, operating process is loaded down with trivial details, detects convenient and swift not.
Utility model content
Technical problem to be solved in the utility model is to provide epidemic meningitis A group, C group, Y group, the antibody combined test card of W135 group IgG.This test card gets final product joint-detection epidemic meningitis A group IgG antibody, epidemic meningitis C group IgG antibody, epidemic meningitis Y group IgG antibody, epidemic meningitis W135 group IgG antibody by single job process, thereby has simplified operating process, has realized the object of fast detecting.
The utility model solves the problems of the technologies described above adopted technical scheme: epidemic meningitis A group, C group, Y group, the antibody combined test card of W135 group IgG, comprise liner, sample pad, fiberglass packing, chromatographic film and absorption pad; Described sample pad, fiberglass packing, chromatographic film and absorption pad join with horizontal direction order and are fixed on described liner; And on described fiberglass packing, be coated with the colored latex particle of tape label; The first detection line, the second detection line, the 3rd detection line, the 4th detection line and control line are set in described chromatographic film; Described the first detection line is that epidemic meningitis A group antigen is fixed in chromatographic film and is formed, described the second detection line is that epidemic meningitis C group antigen is fixed in chromatographic film and is formed, described the 3rd detection line is that epidemic meningitis Y group antigen is fixed in chromatographic film and is formed, and described the 4th detection line is that epidemic meningitis W135 group antigen is fixed in chromatographic film and is formed.
Described the first detection line, the second detection line, the 3rd detection line, the 4th detection line and the direction of control line along sample pad to absorption pad are arranged in order.
In technique scheme, described sample pad and absorption pad all adopt the material with water absorbing properties to be prepared from, and can be commercially available filter paper and make, and preferably the stronger qualitative filter paper of water absorbing properties, is beneficial to detection like this, increase detection speed.Described chromatographic film is commercially available nitrocellulose filter, and nitrocellulose filter claims again NC film, is used as the supporting body of C/T line in colloid gold test paper, is also immunoreactive nidus simultaneously.Nitrocellulose filter model 135s, the 170s or the 180s(definition in seconds that adopt: every 4cm film, the chromatography time of water is how many seconds).Fiberglass packing is a kind of of fiberglass products.Glass fibre is a kind of Inorganic Non-metallic Materials of excellent performance.English former Glassfiber by name, composition is silicon dioxide, aluminium oxide, calcium oxide, boron oxide, magnesium oxide, sodium oxide molybdena etc.It is to take glass bead or discarded glass as raw material is through high temperature melting, wire drawing, doff, the technique such as weave cotton cloth, finally form various product, glass fiber single filament diameter is from several microns to twenties microns, the 1/20-1/50 that is equivalent to a hairline, every bundle fiber precursor all by hundreds of even thousands of monofilament form, usually used as the reinforcing material in multiple material, electrically insulating material and heat-insulating material, circuit substrate etc., are widely used in national economy every field.
The colored latex particle that is evenly coated with tape label on described fiberglass packing, colored latex particle generally adopts red latex particle, and colour developing is obviously like this; The method of the enterprising row labels of latex particle is routine techniques means in this area, and inventor does not repeat at this.The mark of colored latex particle band can be combined by the IgG antibody in human serum, is antigen on detection line.
As preferably, described fiberglass packing is for being coated with the fiberglass packing of the colored latex particle of band staphylococcal protein A (Staphylococal Protein A, SPA) mark.Staphylococcal protein A is a kind of protein from the separation of aureus cell wall.Due to SPA can with the Fc end combination of the IgG of people, mouse, rabbit etc., become a kind of extremely useful instrument in immunology.Described control line is that SPA is fixed on to the control line forming in chromatographic film.Described control line is corresponding with label.
As preferably, described fiberglass packing is the fiberglass packing being coated with the colored latex particle of mouse-anti human IgG labeling of monoclonal antibody.Mouse-anti human IgG monoclonal antibody is a kind of good label.Described control line is that sheep anti-mouse igg antibody is fixed on to the control line forming in chromatographic film.Sheep anti-mouse igg antibody claims again sheep anti-mouse igg polyclonal antibody.Sheep anti-mouse igg (immunoglobulin (Ig)) polyclonal antibody in the quick antibody diagnostic products of colloidal gold method on nitrocellulose filter, with the monoclonal antibody generation specific immune response of colloid gold label and show certain color, form control line and carry out immunodiagnosis.
Further, the main body of described liner is plastic mattress.
Described sample pad length is 20mm, and width is 4mm; Described fiberglass packing length is 10mm, and width is 4mm; Described chromatographic film length is 25mm, and width is 4mm; Described absorption pad length is 20mm, and width is 4mm; Described liner length is 30mm, and width is 4mm, and thickness is 1mm.
Detection principle of the present utility model is: detection of the present utility model is to complete under the promotion of chromatography effect.After blood serum sample is added in sample pad, by chromatography effect, arrive fiberglass packing, IgG antibody in blood serum sample is combined with the colored latex particle of mark and is formed in conjunction with particle, then further to chromatographic film, under the chromatography effect of chromatographic film, in conjunction with particle, move forward, as contained epidemic meningitis A group IgG antibody in serum, C group IgG antibody, Y group IgG antibody, W135 group IgG (inoculates epidemic meningitis A group during antibody, C group, Y group, the antibody that should produce after W135 group's combined vaccine), be combined with corresponding 4 detection lines, the color lines that present particle, unconjugated particle can continue to move forward, be combined with control line and present the color lines of particle, show detection line and control line color line, when do not contained this several antibody in serum, the antigen of particle and corresponding detection line cannot be in conjunction with, and detection line does not develop the color, and for the particle of combination can continue to move forward, is combined presents the color lines of particle with control line, only shows color line of control line.
Because rete is analysed speed, the colored latex particle of tape label is removable complete chromatographic film bar in a few minutes, at once can sentence read result, and as the first detection line and control line all develop the color, serum epidemic meningitis A group IgG antibody is positive; As the second detection line and control line all develop the color, serum epidemic meningitis C group IgG antibody is positive; As the 3rd detection line and control line all develop the color, serum epidemic meningitis Y group IgG antibody is positive; As the 4th detection line and control line all develop the color, serum epidemic meningitis W135 group IgG antibody is positive.Only have line colour developing of control line, serum antibody is negative.
In sum, the beneficial effects of the utility model are:
(1) the utility model test card gets final product joint-detection epidemic meningitis A group IgG antibody, C group IgG antibody, Y group IgG antibody and W135 group IgG antibody by single job process, thereby has simplified operating process, has realized the object of fast detecting; And high specificity, highly sensitive.
(2) when the utility model is used, only physiological saline 1:1 dilution for sample to be checked directly need be added in the sample pad at the utility model well place, operate very easy; Sample is added in the utility model sample pad, and 10 minutes is Observable testing result by view window naked eyes, judge, and approximately 15 minutes consuming time of whole detection, consuming time short.
(3) sample pad length described in the utility model is 20mm, and width is 4mm; Described fiberglass packing length is 10mm, and width is 4mm; Described chromatographic film length is 25mm, and width is 4mm; Described absorption pad length is 20mm, and width is 4mm; Described liner length is 30mm, and width is 4mm, and thickness is 1mm.This size specification, the detection efficiency while not only improving the utility model use, and facilitate the packing of product and transportation.
Accompanying drawing explanation
Fig. 1 is the vertical view of embodiment 1;
Fig. 2 is the cut-open view of embodiment 1.
In figure, the corresponding component names of mark is: 1-sample pad, 2-fiberglass packing, 31-the first detection line, 32-the second detection line, 33-the 3rd detection line, 34-the 4th detection line, 4-control line, 5-chromatographic film, 6-absorption pad, 7-liner.
Embodiment
Below by specific embodiment, and by reference to the accompanying drawings, the technical solution of the utility model is described in further detail.
Embodiment 1
As illustrated in fig. 1 and 2, hepatitis type B virus, the antibody combined test card of b type haemophilus influenzae IgG, comprise liner 7, sample pad 1(filter paper, Shanghai Jin Biao Bioisystech Co., Ltd), fiberglass packing 2(Ahlstrom8964 glass, Shanghai Jie Yi Bioisystech Co., Ltd, model JY-J101), chromatographic film 5(nitrocellulose filter, Millipore company, model 135s) and absorption pad 6(filter paper, Shanghai Jin Biao Bioisystech Co., Ltd).Sample pad 1, fiberglass packing 2, chromatographic film 5 and absorption pad 6 join with horizontal direction order and are fixed on described liner 7.
Described fiberglass packing 2 is for being evenly coated with red latex particle (the red polystyrene latex particle with SPA mark, Shanghai Jie Yi Bioisystech Co., Ltd, model PSR030N) fiberglass packing, the method that latex particle carries out mark is techniques well known, therefore not to repeat here.
The first detection line 31, the second detection line 32, the 3rd detection line 33, the 4th detection line 34 and control line 4 are set in described chromatographic film 5.Described the first detection line 31, the second detection line 32 and control line 4 are arranged in order setting along the direction from sample pad 1 to absorption pad 6 in chromatographic film.Described the first detection line 31 is that epidemic meningitis A group antigen is fixed in chromatographic film 5 and is formed, described the second detection line 32 is that epidemic meningitis C group antigen is fixed in chromatographic film 5 and is formed, described the 3rd detection line 33 is that epidemic meningitis Y group antigen is fixed in chromatographic film 5 and is formed, and described the 4th detection line 34 is that epidemic meningitis W135 group antigen is fixed in chromatographic film 5 and is formed.
Control line 4 is that SPA(is commercially available, Zhengzhou Bai Ji Bioisystech Co., Ltd) be fixed in chromatographic film 5 and form.
In the utility model, described sample pad 1, fiberglass packing 2, chromatographic film 5 and absorption pad 6 join with horizontal direction order.Particularly, the mode that described sample pad 1, fiberglass packing 2, chromatographic film 5 and absorption pad 6 orders are joined is partly overlapping, that is: the tail end of sample pad 1 is overlapped on fiberglass packing 2, the tail end of fiberglass packing 2 is overlapped on the head end of chromatographic film 5, and the head end of absorption pad 6 is overlapped on the tail end of chromatographic film 5.
Further, sample pad 1 length described in the utility model is 20mm, and width is 4mm; Described fiberglass packing 2 length are 10mm, and width is 4mm; Described chromatographic film 5 length are 25mm, and width is 4mm; Described absorption pad 6 length are 20mm, and width is 4mm; Described liner 7 length are 30mm, and width is 4mm, and thickness is 1mm.
Embodiment 2
The present embodiment is substantially the same manner as Example 1, its different piece is only: described fiberglass packing 2 is for being evenly coated with the fiberglass packing with the colored latex particle of mouse-anti human IgG labeling of monoclonal antibody, and described control line 4 is for being fixed on sheep anti-mouse igg antibody in chromatographic film 5 and forming.
Embodiment 3
The present embodiment is substantially the same manner as Example 1, and its different piece is only: the mode that described sample pad 1, fiberglass packing 2, chromatographic film 5 and the order of absorption pad 6 are joined is that head and the tail are connected and do not have overlapping.
Embodiment 4
The present embodiment is substantially the same manner as Example 3, its different piece is only: described fiberglass packing 2 is for being coated with the fiberglass packing with the colored latex particle of mouse-anti human IgG labeling of monoclonal antibody, and described control line 4 is for being fixed on sheep anti-mouse igg antibody in chromatographic film 5 and forming.
Above-described embodiment is a kind of preferably embodiment of the present utility model, not to any pro forma restriction of the utility model, also has other variant and remodeling under the prerequisite that is no more than the technical scheme that claim records.
Claims (1)
1. epidemic meningitis A group, C group, Y group, the antibody combined test card of W135 group IgG, is characterized in that, comprises liner (7), sample pad (1), fiberglass packing (2), chromatographic film (5) and absorption pad (6); Described sample pad (1), fiberglass packing (2), chromatographic film (5) and absorption pad (6) join with horizontal direction order and are fixed on described liner (7); And on described fiberglass packing (2), be coated with the colored latex particle of tape label; The first detection line (31), the second detection line (32), the 3rd detection line (33), the 4th detection line (34) and control line (4) are set in described chromatographic film (5); Described the first detection line (31) is that epidemic meningitis A group antigen is fixed on to the upper formation of chromatographic film (5), described the second detection line (32) is that epidemic meningitis C group antigen is fixed on to the upper formation of chromatographic film (5), described the 3rd detection line (33) is that epidemic meningitis Y group antigen is fixed on to the upper formation of chromatographic film (5), and described the 4th detection line (34) is that epidemic meningitis W135 group antigen is fixed on to the upper formation of chromatographic film (5);
Described the first detection line (31), the second detection line (32), the 3rd detection line (33), the 4th detection line (34) and the direction of control line (4) along sample pad (1) to absorption pad (6) are arranged in order;
Described fiberglass packing (2) is for being coated with the fiberglass packing (2) with the colored latex particle of SPA mark, and described control line (4) goes up for SPA is fixed on chromatographic film (5) control line forming;
Described fiberglass packing (2) is for being coated with the fiberglass packing (2) with the colored latex particle of mouse-anti human IgG labeling of monoclonal antibody, and described control line (4) goes up for sheep anti-mouse igg antibody is fixed on chromatographic film (5) control line (4) forming;
The main body of described liner (7) is plastic mattress;
Described sample pad (1) length is 20mm, and width is 4mm; Described fiberglass packing (2) length is 10mm, and width is 4mm; Described chromatographic film (5) length is 25mm, and width is 4mm; Described absorption pad (6) length is 20mm, and width is 4mm; Described liner (7) length is 30mm, and width is 4mm, and thickness is 1mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420181814.5U CN203981682U (en) | 2014-04-15 | 2014-04-15 | Epidemic meningitis A group, C group, Y group, the antibody combined test card of W135 group IgG |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420181814.5U CN203981682U (en) | 2014-04-15 | 2014-04-15 | Epidemic meningitis A group, C group, Y group, the antibody combined test card of W135 group IgG |
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| Publication Number | Publication Date |
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| CN203981682U true CN203981682U (en) | 2014-12-03 |
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| CN201420181814.5U Expired - Fee Related CN203981682U (en) | 2014-04-15 | 2014-04-15 | Epidemic meningitis A group, C group, Y group, the antibody combined test card of W135 group IgG |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106290848A (en) * | 2015-05-13 | 2017-01-04 | 上海凯创生物技术有限公司 | A kind of meningitis bacterium emulsion technique detection kit |
-
2014
- 2014-04-15 CN CN201420181814.5U patent/CN203981682U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106290848A (en) * | 2015-05-13 | 2017-01-04 | 上海凯创生物技术有限公司 | A kind of meningitis bacterium emulsion technique detection kit |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141203 Termination date: 20180415 |