CN203759023U - Pertussis/diphtheria/tetanus IgG (immunoglobulin G) antibody joint detection card - Google Patents
Pertussis/diphtheria/tetanus IgG (immunoglobulin G) antibody joint detection card Download PDFInfo
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- CN203759023U CN203759023U CN201420182032.3U CN201420182032U CN203759023U CN 203759023 U CN203759023 U CN 203759023U CN 201420182032 U CN201420182032 U CN 201420182032U CN 203759023 U CN203759023 U CN 203759023U
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- chromatographic film
- detection line
- diphtheria
- pertussis
- igg
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Abstract
The utility model discloses a pertussis/diphtheria/tetanus IgG (immunoglobulin G) antibody joint detection card. The detection card is characterized by comprising a pad (7), a sample pad (1), a glass fiber pad (2), a chromatographic film (5) and an absorbent pad (6), wherein the sample pad (1), the glass fiber pad (2), the chromatographic film (5) and the absorbent pad (6) are sequentially connected in the horizontal direction and are fixed on the pad (7); color latex particles with labels are sprayed onto the glass fiber pad (2); a first detection line (31), a second detection line (32), a third detection line (33) and a control line (4) are arranged on the chromatographic film (5). The detection card can be used for jointly detecting the IgG antibodies of bordetella pertussis, corynebacterium diphtheria and clostridium tetani through a once-through operation process, thus simplifying the operation process and achieving the aim of rapid detection.
Description
Technical field
The utility model belongs to technical field of immunoassay, particularly a kind of pertussis, diphtheria, the antibody combined test card of lockjaw IgG.
Background technology
At present, on market, the diagnostic kit of the existing single pertussis IgG antibody of reagent company's production testing, diphtheria IgG antibody or lockjaw IgG antibody, can diagnose respectively three kinds of IgG antibody.But use this class kit to detect the IgG antibody after pertussis, diphtheria and lockjaw triple vaccine, need to buy different kits and detect respectively, complicated operation, length consuming time, expends large.Therefore, although detect separately after pertussis IgG antibody, diphtheria IgG antibody or lockjaw IgG antibody, more comprehensively analyze testing result diagnostic result be there is no to impact, operating process is loaded down with trivial details, detects convenient and swift not.
Utility model content
Technical problem to be solved in the utility model is to provide a kind of pertussis, diphtheria, the antibody combined test card of lockjaw IgG.This test card gets final product joint-detection pertussis, diphtheria and three kinds of IgG antibody of lockjaw by single job process, thereby has simplified operating process, has realized the object of fast detecting.
The utility model solves the problems of the technologies described above adopted technical scheme: pertussis, diphtheria, the antibody combined test card of lockjaw IgG, comprise liner, sample pad, fiberglass packing, chromatographic film and absorption pad; Described sample pad, fiberglass packing, chromatographic film and absorption pad join with horizontal direction order and are fixed on described liner; And on described fiberglass packing, be coated with the colored latex particle of tape label; The first detection line, the second detection line, the 3rd detection line and control line are set in described chromatographic film; Described the first detection line is that Bordetella pertussis toxin or Bordetella pertussis filamentous hemagglutinin are fixed in described chromatographic film and are formed; Described the second detection line is that diphtheria toxoid is fixed in described chromatographic film and is formed; Described the 3rd detection line is that tetanus toxoid is fixed in described chromatographic film and is formed.
Described the first detection line, the second detection line, the 3rd detection line and the direction of control line along sample pad to absorption pad are arranged in order.
In technique scheme, described sample pad and absorption pad all adopt the material with water absorbing properties to be prepared from, and can be commercially available filter paper and make, and preferably the stronger qualitative filter paper of water absorbing properties, is beneficial to detection like this, increase detection speed.Described chromatographic film is commercially available nitrocellulose filter, and nitrocellulose filter claims again NC film, is used as the supporting body of C/T line in colloid gold test paper, is also immunoreactive nidus simultaneously.Nitrocellulose filter model 135s, the 170s or the 180s(definition in seconds that adopt: every 4cm film, the chromatography time of water is how many seconds).Fiberglass packing is the one of fiberglass products.Glass fibre is a kind of Inorganic Non-metallic Materials of excellent performance.English former Glassfiber by name, composition is silicon dioxide, aluminium oxide, calcium oxide, boron oxide, magnesium oxide, sodium oxide molybdena etc.It is through high temperature melting, wire drawing, doff, the technique such as weave cotton cloth taking glass bead or discarded glass as raw material, finally form various product, glass fiber single filament diameter is from several microns to twenties microns, be equivalent to the 1/20-1/50 of a hairline, every bundle fiber precursor all by hundreds of even thousands of monofilament form, usually used as the reinforcing material in multiple material, electrically insulating material and heat-insulating material, circuit substrate etc., are widely used in national economy every field.
The colored latex particle that is evenly coated with tape label on described fiberglass packing, colored latex particle generally adopts red latex particle, and colour developing is obviously like this; The method of the enterprising row labels of latex particle is routine techniques means in this area, and inventor does not repeat at this.The mark of colored latex particle band can be combined by the IgG antibody in human serum, is antigen on detection line.
As preferably, described fiberglass packing is for being coated with the fiberglass packing of colored latex particle of band staphylococcal protein A (Staphylococal Protein A, SPA) mark.Staphylococcal protein A is a kind of protein separating from aureus cell wall.Because SPA can hold combination with the Fc of the IgG of people, mouse, rabbit etc., become a kind of extremely useful instrument in immunology.Described control line is that SPA is fixed on to the control line forming in chromatographic film.Described control line is corresponding with label.
As preferably, described fiberglass packing is the fiberglass packing that is coated with the colored latex particle with mouse-anti human IgG labeling of monoclonal antibody.Mouse-anti human IgG monoclonal antibody is a kind of label preferably.Described control line is that sheep anti-mouse igg antibody is fixed on to the control line forming in chromatographic film.Sheep anti-mouse igg antibody claims again sheep anti-mouse igg polyclonal antibody.Sheep anti-mouse igg (immunoglobulin (Ig)) polyclonal antibody in the quick antibody diagnostic products of colloidal gold method on nitrocellulose filter, with the monoclonal antibody generation specific immune response of colloid gold label and show certain color, form control line and carry out immunodiagnosis.
Detection principle of the present utility model is: detection of the present utility model is to complete under the promotion of chromatography effect.After blood serum sample is added in sample pad, arrive fiberglass packing by chromatography effect, IgG antibody in blood serum sample is combined with the colored latex particle of mark and is formed in conjunction with particle, then further to chromatographic film, under the chromatography effect of chromatographic film, move forward in conjunction with particle, as contained pertussis IgG antibody in serum, when diphtheria IgG antibody and lockjaw IgG antibody, (inoculate pertussis, after diphtheria and lockjaw triple vaccine, should produce this three kinds of antibody), be combined with corresponding 3 detection lines, present the color lines of particle, unconjugated particle can continue to move forward, be combined with control line and present the color lines of particle, show detection line and control line, as do not contained this several antibody in serum time, the antigen of particle and corresponding detection line cannot be in conjunction with, and detection line does not develop the color, and for the particle of combination can continue to move forward, is combined presents the color lines of particle with control line, only shows color line of control line.
Because rete is analysed speed, the colored latex particle of tape label is removable complete chromatographic film bar in a few minutes, at once can sentence read result, and as the first detection line and control line all develop the color, serum pertussis IgG antibody is positive; As the second detection line and control line all develop the color, serum diphtheria IgG antibody is positive; As the 3rd detection line and control line all develop the color, serum lockjaw IgG antibody is positive.Only have line colour developing of control line, serum antibody is negative.
In sum, the beneficial effects of the utility model are:
(1) the utility model test card gets final product joint-detection pertussis, diphtheria and lockjaw IgG antibody by single job process, thereby has simplified operating process, has realized the object of fast detecting; And high specificity, highly sensitive.
(2) when the utility model uses, only need, by physiological saline 1:1 dilution for sample to be checked, directly be added in the utility model sample pad, operate very easy; Sample is added in the utility model sample pad, and within 10 minutes, naked eyes are Observable testing result, judge, and approximately 15 minutes consuming time of whole detection, consuming time short.
Brief description of the drawings
Fig. 1 is the vertical view of embodiment 1;
Fig. 2 is the cut-open view of embodiment 1.
In figure, the corresponding component names of mark is: 1-sample pad, 2-fiberglass packing, 31-the first detection line, 32-the second detection line, 33-the 3rd detection line, 4-control line, 5-chromatographic film, 6-absorption pad, 7-liner.
Embodiment
Below by specific embodiment, and by reference to the accompanying drawings, the technical solution of the utility model is described in further detail.
Embodiment 1
As illustrated in fig. 1 and 2, pertussis, diphtheria, the antibody combined test card of lockjaw IgG, comprise liner 7, sample pad 1(filter paper, Shanghai Jin Biao Bioisystech Co., Ltd), fiberglass packing 2(Ahlstrom8964 glass, the outstanding Bioisystech Co., Ltd in Shanghai, model JY-J101), chromatographic film 5(nitrocellulose filter, Millipore company, model 135s) and absorption pad 6(filter paper, Shanghai Jin Biao Bioisystech Co., Ltd).Sample pad 1, fiberglass packing 2, chromatographic film 5 and absorption pad 6 join with horizontal direction order and are fixed on described liner 7.
Described fiberglass packing 2 is for being evenly coated with red latex particle (the red polystyrene latex particle with SPA mark, the outstanding Bioisystech Co., Ltd in Shanghai, model PSR030N) fiberglass packing, the method that latex particle carries out mark is techniques well known, therefore not to repeat here.
The first detection line 31, the second detection line 32, the 3rd detection line 33 and control line 4 are set in described chromatographic film 5.Described the first detection line 31, the second detection line 32, the 3rd detection line 33 and control line 4 in chromatographic film along being arranged in order setting from sample pad 1 to the direction of absorption pad 6.Described the first detection line 31 is that Bordetella pertussis toxin or Bordetella pertussis filamentous hemagglutinin are fixed on to formation in described chromatographic film 5; Described the second detection line 32 is that diphtheria toxoid is fixed in described chromatographic film 5 and is formed; Described the 3rd detection line 33 is that tetanus toxoid is fixed in described chromatographic film 5 and is formed.
Control line 4 is that SPA(is commercially available, Zhengzhou Bai Ji Bioisystech Co., Ltd) be fixed in chromatographic film 5 and form.
In the utility model, described sample pad 1, fiberglass packing 2, chromatographic film 5 and absorption pad 6 join with horizontal direction order.Particularly, the mode that described sample pad 1, fiberglass packing 2, chromatographic film 5 and absorption pad 6 orders are joined is partly overlapping, that is: the tail end of sample pad 1 is overlapped on fiberglass packing 2, the tail end of fiberglass packing 2 is overlapped on the head end of chromatographic film 5, and the head end of absorption pad 6 is overlapped on the tail end of chromatographic film 5.
Embodiment 2
The present embodiment is substantially the same manner as Example 1, its different piece is only: described fiberglass packing 2 is for being evenly coated with the fiberglass packing of the colored latex particle with mouse-anti human IgG labeling of monoclonal antibody, and described control line 4 is for being fixed on sheep anti-mouse igg antibody in chromatographic film 5 and forming.
Embodiment 3
The present embodiment is substantially the same manner as Example 1, and its different piece is only: the mode that described sample pad 1, fiberglass packing 2, chromatographic film 5 and the order of absorption pad 6 are joined is that head and the tail are connected and do not have overlapping.
Embodiment 4
The present embodiment is substantially the same manner as Example 3, its different piece is only: described fiberglass packing 2 is for being coated with the fiberglass packing of the colored latex particle with mouse-anti human IgG labeling of monoclonal antibody, and described control line 4 is for being fixed on sheep anti-mouse igg antibody in chromatographic film 5 and forming.
Above-described embodiment is preferably embodiment of one of the present utility model, not to any pro forma restriction of the utility model, also has other variant and remodeling under the prerequisite that is no more than the technical scheme that claim records.
Claims (4)
1. pertussis, diphtheria, the antibody combined test card of lockjaw IgG, is characterized in that, comprises liner (7), sample pad (1), fiberglass packing (2), chromatographic film (5) and absorption pad (6); Described sample pad (1), fiberglass packing (2), chromatographic film (5) and absorption pad (6) join with horizontal direction order and are fixed on described liner (7); And on described fiberglass packing (2), be coated with the colored latex particle of tape label; The first detection line (31), the second detection line (32), the 3rd detection line (33) and control line (4) are set in described chromatographic film (5);
Described the first detection line (31) is that Bordetella pertussis toxin or Bordetella pertussis filamentous hemagglutinin are fixed on to the upper formation of described chromatographic film (5); Described the second detection line (32) is that diphtheria toxoid is fixed on to the upper formation of described chromatographic film (5); Described the 3rd detection line (33) is that tetanus toxoid is fixed on to the upper formation of described chromatographic film (5).
2. pertussis according to claim 1, diphtheria, the antibody combined test card of lockjaw IgG, it is characterized in that, described the first detection line (31), the second detection line (32), the 3rd detection line (33) and control line (4) are arranged in order to the direction of absorption pad (6) along sample pad (1).
3. pertussis according to claim 2, diphtheria, the antibody combined test card of lockjaw IgG, it is characterized in that, described fiberglass packing (2) is for being coated with the fiberglass packing (2) of the colored latex particle with SPA mark, and described control line (4) goes up for SPA is fixed on chromatographic film (5) control line forming.
4. pertussis according to claim 2, diphtheria, the antibody combined test card of lockjaw IgG, it is characterized in that, described fiberglass packing (2) is for being coated with the fiberglass packing (2) of the colored latex particle with mouse-anti human IgG labeling of monoclonal antibody, and described control line (4) goes up for sheep anti-mouse igg antibody is fixed on chromatographic film (5) control line (4) forming.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420182032.3U CN203759023U (en) | 2014-04-15 | 2014-04-15 | Pertussis/diphtheria/tetanus IgG (immunoglobulin G) antibody joint detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420182032.3U CN203759023U (en) | 2014-04-15 | 2014-04-15 | Pertussis/diphtheria/tetanus IgG (immunoglobulin G) antibody joint detection card |
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| CN203759023U true CN203759023U (en) | 2014-08-06 |
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| CN201420182032.3U Expired - Fee Related CN203759023U (en) | 2014-04-15 | 2014-04-15 | Pertussis/diphtheria/tetanus IgG (immunoglobulin G) antibody joint detection card |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108254556A (en) * | 2018-03-15 | 2018-07-06 | 北京科兴中维生物技术有限公司 | A kind of pertussis toxin detection kit and its application |
-
2014
- 2014-04-15 CN CN201420182032.3U patent/CN203759023U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108254556A (en) * | 2018-03-15 | 2018-07-06 | 北京科兴中维生物技术有限公司 | A kind of pertussis toxin detection kit and its application |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140806 Termination date: 20180415 |