CN203870109U - Hepatitis a virus and hepatitis b virus IgG antibody combined detection card - Google Patents
Hepatitis a virus and hepatitis b virus IgG antibody combined detection card Download PDFInfo
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- CN203870109U CN203870109U CN201420181380.9U CN201420181380U CN203870109U CN 203870109 U CN203870109 U CN 203870109U CN 201420181380 U CN201420181380 U CN 201420181380U CN 203870109 U CN203870109 U CN 203870109U
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Abstract
The utility model discloses a hepatitis a virus and hepatitis b virus IgG antibody combined detection card. The detection card comprises a liner (7), a sample pad (1), a fiberglass pad (2), a chromatography film (5) and an absorption pad (6), wherein the sample pad (1), the fiberglass pad (2), the chromatography film (5) and the absorption pad (6) are connected sequentially in the horizontal direction and fixed to the liner (7), colorful latex particles with marks are sprayed on the fiberglass pad (2), a first detection line (31), a second detection line (32) and a contrast line (4) are arranged on the chromatography film (5), the first detection line (31) is composed of hepatitis a virus antigens, and the second detection line (32) is composed of hepatitis b virus antigens. According to the detection card, the hepatitis a virus and hepatitis b virus IgG antibodies can be detected combinedly through one operation, so that the operation process is simplified, and the purpose of fast detection can be realized.
Description
Technical field
The utility model belongs to technical field of immunoassay, particularly a kind of hepatitis A, B virus IgG antibody combined detection card.
Background technology
Virus hepatitis is that the hepatitis infringement of take being caused by multiple hepatitis virus is the acute or continuation infectious disease of main general, all has popular all over the world.China most areas belongs to the high epidemic regions of hepatitis A, and annual report of infectious disease surpasses 1,000,000 examples, and the overwhelming majority is preschool child.Along with the improvement of expanding economy in recent years and sanitary condition, hepatitis A popular appearance moves on age of onset, be in a bad way, and the feature that case fatality rate increases.Hepatitis B is global public health problem, and China belongs to the high Endemic Area of hepatitis B, and Population carriage is high.The use of vaccine, has reduced the incidence of hepatitis A, B effectively.
The first and second hepatitis combined vaccines are first, hepatitis B dual specificity protective values, prevent this two kinds of diseases simultaneously.
At present, on market, the existing diagnostic kit for single hepatitis A virus IgG antibody or hepatitis type B virus IgG antibody, can diagnose respectively this two kinds of IgG antibody.But use this class monospecific antibody detection kit, detect the IgG antibody after the first and second liver combined vaccines, need to buy different kits and detect respectively, complicated operation, length consuming time, expends large.Therefore, although detect separately after hepatitis A virus IgG antibody and hepatitis type B virus IgG antibody, more comprehensively analyze testing result diagnostic result be there is no to impact, operating process is loaded down with trivial details, detects convenient and swift not.
Utility model content
Technical problem to be solved in the utility model is to provide hepatitis A, B virus IgG antibody combined detection card.This test card gets final product joint-detection hepatitis A, B virus IgG antibody by single job process, thereby has simplified operating process, has realized the object of fast detecting.
The utility model solves the problems of the technologies described above adopted technical scheme: hepatitis A, B virus IgG antibody combined detection card, comprises liner, sample pad, fiberglass packing, chromatographic film and absorption pad; Described sample pad, fiberglass packing, chromatographic film and absorption pad join with horizontal direction order and are fixed on described liner; And on described fiberglass packing, be coated with the colored latex particle of tape label; The first detection line, the second detection line and control line are set in described chromatographic film; Described the first detection line consists of hepatitis A viral antigen, and described the second detection line consists of hepatitis B virus antigen.
Described the first detection line, the second detection line and the direction of control line along sample pad to absorption pad are arranged in order.
In technique scheme, described sample pad and absorption pad all adopt the material with water absorbing properties to be prepared from, and can be commercially available filter paper and make, and preferably the stronger qualitative filter paper of water absorbing properties, is beneficial to detection like this, increase detection speed.Described chromatographic film is commercially available nitrocellulose filter, and nitrocellulose filter claims again NC film, is used as the supporting body of C/T line in colloid gold test paper, is also immunoreactive nidus simultaneously.Nitrocellulose filter model 135s, the 170s or the 180s(definition in seconds that adopt: every 4cm film, the chromatography time of water is how many seconds).Fiberglass packing is a kind of of fiberglass products.Glass fibre is a kind of Inorganic Non-metallic Materials of excellent performance.English former Glassfiber by name, composition is silicon dioxide, aluminium oxide, calcium oxide, boron oxide, magnesium oxide, sodium oxide molybdena etc.It is to take glass bead or discarded glass as raw material is through high temperature melting, wire drawing, doff, the technique such as weave cotton cloth, finally form various product, glass fiber single filament diameter is from several microns to twenties microns, the 1/20-1/50 that is equivalent to a hairline, every bundle fiber precursor all by hundreds of even thousands of monofilament form, usually used as the reinforcing material in multiple material, electrically insulating material and heat-insulating material, circuit substrate etc., are widely used in national economy every field.
The colored latex particle that is evenly coated with tape label on described fiberglass packing, colored latex particle generally adopts red latex particle, and colour developing is obviously like this; The method of the enterprising row labels of latex particle is routine techniques means in this area, and inventor does not repeat at this.The mark of colored latex particle band can be combined by the IgG antibody in human serum, is antigen on detection line.
As preferably, described fiberglass packing is for being coated with the fiberglass packing of the colored latex particle of band staphylococcal protein A (Staphylococal Protein A, SPA) mark.Staphylococcal protein A is a kind of protein from the separation of aureus cell wall.Due to SPA can with the Fc end combination of the IgG of people, mouse, rabbit etc., become a kind of extremely useful instrument in immunology.Described control line is that SPA is fixed on to the control line forming in chromatographic film.Described control line is corresponding with label.
As preferably, described fiberglass packing is the fiberglass packing being coated with the colored latex particle of mouse-anti human IgG labeling of monoclonal antibody.Mouse-anti human IgG monoclonal antibody is a kind of good label.Described control line is that sheep anti-mouse igg antibody is fixed on to the control line forming in chromatographic film.Sheep anti-mouse igg antibody claims again sheep anti-mouse igg polyclonal antibody.Sheep anti-mouse igg (immunoglobulin (Ig)) polyclonal antibody in the quick antibody diagnostic products of colloidal gold method on nitrocellulose filter, with the monoclonal antibody generation specific immune response of colloid gold label and show certain color, form control line and carry out immunodiagnosis.
Further, described hepatitis A, B virus IgG antibody combined detection card,, also comprising housing, described liner is placed in housing; The top of described housing is provided with view window corresponding to the position of chromatographic film, corresponding to the position of sample pad, is provided with well.
Described housing is rigid plastic.
Described view window is square opening or slide.
Detection principle of the present utility model is: detection of the present utility model is to complete under the promotion of chromatography effect.After blood serum sample is added in sample pad, by chromatography effect, arrive fiberglass packing, IgG antibody in blood serum sample is combined with the colored latex particle of mark and is formed in conjunction with particle, then further to chromatographic film, under the chromatography effect of chromatographic film, in conjunction with particle, move forward, (inoculate hepatitis A when contained hepatitis A virus IgG antibody and hepatitis type B virus IgG antibody in serum, after hepatitis B combined vaccine, should produce this two kinds of antibody), be combined with corresponding 2 detection lines, the color lines that present particle, unconjugated particle can continue to move forward, be combined with control line and present the color lines of particle, show detection line and control line color line, when do not contained this several antibody in serum, the antigen of particle and corresponding detection line cannot be in conjunction with, and detection line does not develop the color, and for the particle of combination can continue to move forward, is combined presents the color lines of particle with control line, only shows color line of control line.
Because rete is analysed speed, the colored latex particle of tape label is removable complete chromatographic film bar in a few minutes, at once can sentence read result, and as the first detection line and control line all develop the color, hepatitis A virus IgG antibody is positive; As the second detection line and control line all develop the color, serum hepatitis B virus IgG antibody is positive.Only have line colour developing of control line, serum antibody is negative.
In sum, the beneficial effects of the utility model are:
(1) the utility model test card gets final product joint-detection hepatitis A virus IgG antibody and hepatitis type B virus IgG antibody by single job process, thereby has simplified operating process, has realized the object of fast detecting; And high specificity, highly sensitive.
(2) when the utility model is used, only physiological saline 1:1 dilution for sample to be checked directly need be added in the sample pad at the utility model well place, operate very easy; Sample is added in the utility model sample pad, and 10 minutes is Observable testing result by view window naked eyes, judge, and approximately 15 minutes consuming time of whole detection, consuming time short.
Accompanying drawing explanation
Fig. 1 is the vertical view of embodiment 1;
Fig. 2 is the cut-open view of embodiment 1.
Fig. 3 is the structural representation of embodiment 5.
In figure, the corresponding component names of mark is: 1-sample pad, 2-fiberglass packing, 31-the first detection line, 32-the second detection line, 4-control line, 5-chromatographic film, 6-absorption pad, 7-liner, 8-housing, 9-view window, 10-well.
Embodiment
Below by specific embodiment, and by reference to the accompanying drawings, the technical solution of the utility model is described in further detail.
Embodiment 1
As illustrated in fig. 1 and 2, hepatitis A, B virus IgG antibody combined detection card, comprise liner 7, sample pad 1(filter paper, Shanghai Jin Biao Bioisystech Co., Ltd), fiberglass packing 2(Ahlstrom8964 glass, Shanghai Jie Yi Bioisystech Co., Ltd, model JY-J101), chromatographic film 5(nitrocellulose filter, Millipore company, model 135s) and absorption pad 6(filter paper, Shanghai Jin Biao Bioisystech Co., Ltd).Sample pad 1, fiberglass packing 2, chromatographic film 5 and absorption pad 6 join with horizontal direction order and are fixed on described liner 7.
Described fiberglass packing 2 is for being evenly coated with red latex particle (the red polystyrene latex particle with SPA mark, Shanghai Jie Yi Bioisystech Co., Ltd, model PSR030N) fiberglass packing, the method that latex particle carries out mark is techniques well known, therefore not to repeat here.
The first detection line 31, the second detection line 32 and control line 4 are set in described chromatographic film 5.Described the first detection line 31, the second detection line 32 and control line 4 are arranged in order setting along the direction from sample pad 1 to absorption pad 6 in chromatographic film.The first detection line 31 is that the purifying hepatitis A viral antigen of deactivation is fixed in chromatographic film 5 and is formed, and the second detection line 32 is that the purifying hepatitis B virus antigen of deactivation is fixed in chromatographic film 5 and is formed.
Control line 4 is that SPA(is commercially available, Zhengzhou Bai Ji Bioisystech Co., Ltd) be fixed in chromatographic film 5 and form.
In the utility model, described sample pad 1, fiberglass packing 2, chromatographic film 5 and absorption pad 6 join with horizontal direction order.Particularly, the mode that described sample pad 1, fiberglass packing 2, chromatographic film 5 and absorption pad 6 orders are joined is partly overlapping, that is: the tail end of sample pad 1 is overlapped on fiberglass packing 2, the tail end of fiberglass packing 2 is overlapped on the head end of chromatographic film 5, and the head end of absorption pad 6 is overlapped on the tail end of chromatographic film 5.
Embodiment 2
The present embodiment is substantially the same manner as Example 1, its different piece is only: described fiberglass packing 2 is for being evenly coated with the fiberglass packing with the colored latex particle of mouse-anti human IgG labeling of monoclonal antibody, and described control line 4 is for being fixed on sheep anti-mouse igg antibody in chromatographic film 5 and forming.
Embodiment 3
The present embodiment is substantially the same manner as Example 1, and its different piece is only: the mode that described sample pad 1, fiberglass packing 2, chromatographic film 5 and the order of absorption pad 6 are joined is that head and the tail are connected and do not have overlapping.
Embodiment 4
The present embodiment is substantially the same manner as Example 3, its different piece is only: described fiberglass packing 2 is for being coated with the fiberglass packing with the colored latex particle of mouse-anti human IgG labeling of monoclonal antibody, and described control line 4 is for being fixed on sheep anti-mouse igg antibody in chromatographic film 5 and forming.
Embodiment 5
As shown in Figure 3, the present embodiment is substantially the same manner as Example 1, its different piece is only: the utility model also comprises housing 8, described liner 7, sample pad 1, fiberglass packing 2, chromatographic film 5 and absorption pad 6 are all placed in housing 8, the top of described housing 8 is provided with view window 9 corresponding to the position of chromatographic film 5, corresponding to the position of sample pad 1, is provided with well 10.And described housing is rigid plastic, described view window is square opening or slide.During use, by well 10, sample is added in sample pad 1.
Above-described embodiment is a kind of preferably embodiment of the present utility model, not to any pro forma restriction of the utility model, also has other variant and remodeling under the prerequisite that is no more than the technical scheme that claim records.
Claims (7)
1. hepatitis A, B virus IgG antibody combined detection card, is characterized in that, comprises liner (7), sample pad (1), fiberglass packing (2), chromatographic film (5) and absorption pad (6); Described sample pad (1), fiberglass packing (2), chromatographic film (5) and absorption pad (6) join with horizontal direction order and are fixed on described liner (7); And on described fiberglass packing (2), be coated with the colored latex particle of tape label; The first detection line (31), the second detection line (32) and control line (4) are set in described chromatographic film (5); Described the first detection line (31) consists of hepatitis A viral antigen, and described the second detection line (32) consists of hepatitis B virus antigen.
2. hepatitis A, B virus IgG antibody combined detection card according to claim 1, it is characterized in that, described the first detection line (31), the second detection line (32) and the direction of control line (4) along sample pad (1) to absorption pad (6) are arranged in order.
3. hepatitis A, B virus IgG antibody combined detection card according to claim 2, it is characterized in that, described fiberglass packing (2) is for being coated with the fiberglass packing (2) with the colored latex particle of SPA mark, and described control line (4) goes up for SPA is fixed on chromatographic film (5) control line forming.
4. hepatitis A, B virus IgG antibody combined detection card according to claim 2, it is characterized in that, described fiberglass packing (2) is for being coated with the fiberglass packing (2) with the colored latex particle of mouse-anti human IgG labeling of monoclonal antibody, and described control line (4) goes up for sheep anti-mouse igg antibody is fixed on chromatographic film (5) control line (4) forming.
5. hepatitis A, B virus IgG antibody combined detection card according to claim 2, is characterized in that, also comprises housing (8), and described liner (7) is placed in housing (8); The top of described housing (8) is provided with view window (9) corresponding to the position of chromatographic film (5), corresponding to the position of sample pad (1), is provided with well (10).
6. hepatitis A, B virus IgG antibody combined detection card according to claim 5, is characterized in that, described housing (8) is rigid plastic.
7. hepatitis A, B virus IgG antibody combined detection card according to claim 6, is characterized in that, described view window (9) is square opening or slide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420181380.9U CN203870109U (en) | 2014-04-15 | 2014-04-15 | Hepatitis a virus and hepatitis b virus IgG antibody combined detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420181380.9U CN203870109U (en) | 2014-04-15 | 2014-04-15 | Hepatitis a virus and hepatitis b virus IgG antibody combined detection card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203870109U true CN203870109U (en) | 2014-10-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420181380.9U Expired - Fee Related CN203870109U (en) | 2014-04-15 | 2014-04-15 | Hepatitis a virus and hepatitis b virus IgG antibody combined detection card |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203870109U (en) |
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2014
- 2014-04-15 CN CN201420181380.9U patent/CN203870109U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141008 Termination date: 20180415 |