CN202854142U - Pertussis IgG antibody test card - Google Patents
Pertussis IgG antibody test card Download PDFInfo
- Publication number
- CN202854142U CN202854142U CN 201220591890 CN201220591890U CN202854142U CN 202854142 U CN202854142 U CN 202854142U CN 201220591890 CN201220591890 CN 201220591890 CN 201220591890 U CN201220591890 U CN 201220591890U CN 202854142 U CN202854142 U CN 202854142U
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- pertussis
- igg antibody
- chromatographic film
- control line
- test card
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Abstract
The utility model discloses a pertussis IgG antibody test card. The pertussis IgG antibody test card comprises a gasket (7), and a sample pad (1), a glass fiber mat (2), a chromatography membrane (5) and an absorption pad (6) which are sequentially connected in horizontal direction and fixedly arranged on the gasket (7); color latex granules with marks are coated on the glass fiber mat (2); the chromatography membrane (5) is provided with a test line (3) which is formed by fixing bordetella pertussis toxin or bordetella pertussis filamentous hemagglutinin on the chromatography membrane (5). The pertussis IgG antibody test card can fast and effectively detect whether a specificity protection antibody is generated in a human body inoculated with vaccine.
Description
Technical field
The utility model belongs to technical field of immunoassay, particularly a kind of pertussis IgG antibody test card for whether producing the IgG antibody of anti-Bordetella pertussis in the human body behind the fast detecting inoculation pertussis seedling.
Background technology
Pertussis vaccine is the acellular component vaccine made from a kind of surface protein filamentous hemagglutinin (Filamentous Hemagglutinin, FHA) of Bordetella pertussis toxoid (Pertussis Toxin, PT) and Bordetella pertussis, is used for the prevention pertussis.
Adopt euzymelinked immunosorbent assay (ELISA) (commercial reagent box for detection of whether producing anti-Bordetella pertussis IgG antibody in the human body behind the inoculation pertussis vaccine at present more, pertussis IgG TPPA kit (euzymelinked immunosorbent assay (ELISA))), the method is after the human serum with the immunity inoculation pertussis vaccine dilutes, be added in the microwell plate of coated pertussis fraction antigen, 37 ℃ hatch 1 hour after, washing for several times, add anti-human IgG enzyme labeling thing, again through washing, add the substrate colour developing, the size of OD value is judged the IgG antibody that whether contains anti-Bordetella pertussis in the human serum per sample.
The technical matters that the method exists: 1, complex operation: test procedure is more.Serum to be checked or blood plasma need dilute, and the first two reactions steps need be carried out the several washing, and last microplate reader reads the OD value; 2, length consuming time: three steps (adding sample, enzyme labeling thing, substrate) all need 37 ℃ and hatch in the process of the test, add wash time, and whole test needs 3 hours consuming time.
The utility model content
Technical problem to be solved in the utility model provides a kind of pertussis IgG antibody test card, and whether its can fast detecting produces anti-pertussal IgG antibody in the human body behind inoculation pertussis vaccine, detects easy and simple to handlely, and production cost is low.
The utility model solves the problems of the technologies described above the technical scheme that adopts: a kind of pertussis IgG antibody test card comprises liner, sample pad, fiberglass packing, chromatographic film and absorption pad; Described sample pad, fiberglass packing, chromatographic film and absorption pad join with the horizontal direction order and are fixed on the described liner; And be coated with the colored latex particle of tape label on the described fiberglass packing; On the described chromatographic film test wire is set, described test wire is Bordetella pertussis toxin or Bordetella pertussis filamentous hemagglutinin to be fixed on the chromatographic film form.
As preferably, control line also is set on the described chromatographic film, and described test wire is between described sample pad and described control line.
As preferably, the main body of described liner is plastic mattress.
As preferably, described sample pad length is 20mm, and width is 5mm; Described fiberglass packing length is 10mm, and width is 5mm; Described chromatographic film length is 25mm, and width is 5mm; Described absorption pad length is 20mm, and width is 5mm; Described liner length is 30mm, and width is 5mm, and thickness is 1mm.
In the technique scheme, described sample pad and absorption pad all adopt the material with water absorbing properties to be prepared from, and can be commercially available filter paper and make, and the preferred stronger qualitative filter paper of water absorbing properties is beneficial to detection like this, increases detection speed.Described chromatographic film is commercially available nitrocellulose filter, and nitrocellulose filter claims again the NC film, is used as the supporting body of C/T line in colloid gold test paper, also is immunoreactive nidus simultaneously.Nitrocellulose filter model 135s, 170s or the definition of 180s(take second as unit adopted: every 4cm film, what seconds the chromatography time of water is).Fiberglass packing is a kind of of fiberglass products.Glass fibre is a kind of Inorganic Non-metallic Materials of excellent performance.English former Glassfiber by name, composition is silicon dioxide, aluminium oxide, calcium oxide, boron oxide, magnesium oxide, sodium oxide molybdena etc.It is through high temperature melting, wire drawing, doff, the technique such as weave cotton cloth take glass bead or discarded glass as raw material, form at last various product, the glass fiber single filament diameter is from several microns to twenties microns, the 1/20-1/50 that is equivalent to a hairline, every bundle fiber precursor all is comprised of hundreds of even thousands of monofilament, usually as reinforcing material, electrically insulating material and heat-insulating material in the multiple material, circuit substrate etc. are widely used in the national economy every field.
Evenly be coated with the colored latex particle of tape label on the described fiberglass packing, colored latex particle generally adopts red latex particle, and colour developing obviously like this; The method of the enterprising row labels of latex particle is the routine techniques means in this area, and the inventor does not do at this and gives unnecessary details.The mark of colored latex particle band can be combined by the IgG antibody in human serum, is antigen on the test wire.
As preferably, described fiberglass packing is the fiberglass packing of the colored latex particle that is coated with band staphylococcal protein A (Staphylococal Protein A, SPA) mark.Staphylococcal protein A is a kind of protein that separates from the aureus cell wall.Since SPA can with the Fc end combination of the IgG of people, mouse, rabbit etc., become a kind of extremely useful instrument on the immunology.Described control line is that SPA is fixed on the control line that forms on the chromatographic film.Described control line is corresponding with label.
As preferably, described fiberglass packing is the fiberglass packing that is coated with the colored latex particle of mouse-anti human IgG labeling of monoclonal antibody.Mouse-anti human IgG monoclonal antibody is a kind of preferably label.Described control line is that sheep anti-mouse igg antibody is fixed on the control line that forms on the chromatographic film.Sheep anti-mouse igg antibody claims again the sheep anti-mouse igg polyclonal antibody.Sheep anti-mouse igg (immunoglobulin (Ig)) polyclonal antibody in the quick antibody diagnostic products of colloidal gold method on nitrocellulose filter, with the monoclonal antibody generation specific immune response of colloid gold label and show certain color, form control line and carry out immunodiagnosis.
Detection principle of the present utility model is: detection of the present utility model is to finish under the promotion of chromatography effect.After blood serum sample is added on the sample pad, arrive fiberglass packing by the chromatography effect, IgG antibody in the blood serum sample is combined with the colored latex particle of mark and is formed in conjunction with particle, then further to chromatographic film, under the chromatography effect of chromatographic film, move forward in conjunction with particle, when containing the antibody of water resistant acne herpes zoster virus in the serum (should produce this antibody after inoculating chicken pox vaccine), be combined with corresponding test wire, the color lines that present particle, unconjugated particle can continue to move forward, and is combined the color lines that present particle with control line, namely shows two color lines of test wire and control line; When not containing the antibody of water resistant acne herpes zoster virus in the serum; the antigen of particle and corresponding test wire can't in conjunction with, test wire does not develop the color, for the particle of combination can continue to move forward; be combined the color lines that present particle with control line, namely only show color line of control line.
Because rete is analysed speed, the colored latex particle of tape label is removable complete chromatographic film bar in a few minutes, at once can sentence read result, all develop the color such as test wire and control line, and serum antibody is positive; Only have line colour developing of control line, serum antibody is negative.
In sum, the beneficial effects of the utility model are:
(1) anti-pertussis IgG antibody is the species specificity protection antibody that produces in the human body behind the inoculation pertussis vaccine.When only having this species specificity protection antibody in the human body, could be when human body touches Bordetella pertussis the toxin of Bordetella pertussis and generation thereof be neutralized and not infected.The utility model pertussis IgG antibody test card can detect whether produced this species specificity protection antibody after people's vaccine inoculation in the body fast and effectively.
When (2) the utility model uses, only need sample to be checked with physiological saline 1:1 dilution, directly be added on the utility model sample pad and get final product, operate very easy; Sample is added on the utility model sample pad, and naked eyes were the Observable testing result in 10 minutes, judge, and approximately 15 minutes consuming time of whole detection, consuming time short.
(3) sample pad length described in the utility model is 20mm, and width is 5mm; Described fiberglass packing length is 10mm, and width is 5mm; Described chromatographic film length is 25mm, and width is 5mm; Described absorption pad length is 20mm, and width is 5mm; Described liner length is 30mm, and width is 5mm, and thickness is 1mm.This size specification, the detection efficiency when not only improving the utility model use, and make things convenient for the packing of product and transportation.
Description of drawings
Fig. 1 is the vertical view of embodiment 1;
Fig. 2 is the cut-open view of embodiment 1.
The corresponding component names of mark is among the figure: 1-sample pad, 2-fiberglass packing, 3-test wire, 4-control line, 5-chromatographic film, 6-absorption pad, 7-liner.
Embodiment
Below by specific embodiment, and by reference to the accompanying drawings, the technical solution of the utility model is described in further detail.
As illustrated in fig. 1 and 2, a kind of pertussis IgG antibody test card, comprise liner 7, the main body of liner 7 is plastic mattress, the surface of plastic mattress is coated with adhesive sticker, along continuous straight runs fixedly has the sample pad 1(filter paper of water absorbing properties successively on the described liner 7, Shanghai gold mark Bioisystech Co., Ltd), fiberglass packing 2(Ahlstrom8964 glass, the outstanding Bioisystech Co., Ltd in Shanghai, model JY-J101), chromatographic film 5(nitrocellulose filter, Millipore company, model 135s) and have the absorption pad 6(filter paper of water absorbing properties, Shanghai gold mark Bioisystech Co., Ltd).
Described fiberglass packing 2 is for evenly being coated with red latex particle (the red polystyrene latex particle with the SPA mark, the outstanding Bioisystech Co., Ltd in Shanghai, model PSR030N) method that fiberglass packing, latex particle carry out mark is techniques well known, and therefore not to repeat here.
Described test wire 3 is that the Bordetella pertussis toxin (Lanzhou Institute of Biological Products Co., Ltd.) of the purifying of deactivation or Bordetella pertussis filamentous hemagglutinin (Lanzhou Institute of Biological Products Co., Ltd.) are fixed on formation on the chromatographic film 5.
Described control line 4 is that SPA(is commercially available, Zhengzhou hundred basic Bioisystech Co., Ltd) be fixed on the chromatographic film 5 and form.Purifying pertussis bacillus toxin and Bordetella pertussis filamentous hemagglutinin, SPA fixing means are techniques well known, and therefore not to repeat here.
In the utility model, described sample pad 1, fiberglass packing 2, chromatographic film 5 and absorption pad 6 join with the horizontal direction order.Particularly, the mode that described sample pad 1, fiberglass packing 2, chromatographic film 5 and absorption pad 6 orders are joined is partly overlapping, that is: the tail end of sample pad 1 is overlapped on the fiberglass packing 2, the tail end of fiberglass packing 2 is overlapped on the head end of chromatographic film 5, and the head end of absorption pad 6 is overlapped on the tail end of chromatographic film 5.
Further, sample pad 1 length described in the utility model is 20mm, and width is 5mm; Described fiberglass packing 2 length are 10mm, and width is 5mm; Described chromatographic film 5 length are 25mm, and width is 5mm; Described absorption pad 6 length are 20mm, and width is 5mm; Described liner 7 length are 30mm, and width is 5mm, and thickness is 1mm.
The present embodiment is substantially the same manner as Example 1, its different piece only is: described fiberglass packing 2 is for evenly being coated with the fiberglass packing with the colored latex particle of mouse-anti human IgG labeling of monoclonal antibody, and described control line 4 forms for sheep anti-mouse igg antibody is fixed on the chromatographic film 5.
The present embodiment is substantially the same manner as Example 1, and its different piece only is: the mode that the order that described sample pad 1, fiberglass packing 2, chromatographic film 5 are connected with absorption pad is joined is that head and the tail connect and do not have overlapping.
The present embodiment is substantially the same manner as Example 3, its different piece only is: described fiberglass packing 2 is for being coated with the fiberglass packing with the colored latex particle of mouse-anti human IgG labeling of monoclonal antibody, and described control line 4 forms for sheep anti-mouse igg antibody is fixed on the chromatographic film 5.
When the utility model uses, only need sample to be checked with physiological saline 1:1 dilution, directly be added on the sample pad of the present utility model and get final product, operate very easy; Sample is added on the utility model sample pad, and naked eyes were the Observable testing result in 10 minutes, judges, all develops the color such as test wire 3 and control line 4, and serum antibody is positive; Only have 4 one line colour developings of control line, serum antibody is negative.Approximately 15 minutes consuming time of whole detection, consuming time short.
Above-described embodiment is a kind of better embodiment of the present utility model, is not to any pro forma restriction of the utility model, also has other variant and remodeling under the prerequisite that is no more than the technical scheme that claim puts down in writing.
Claims (6)
1. a pertussis IgG antibody test card is characterized in that, comprises liner (7), sample pad (1), fiberglass packing (2), chromatographic film (5) and absorption pad (6); Described sample pad (1), fiberglass packing (2), chromatographic film (5) and absorption pad (6) join with the horizontal direction order and are fixed on the described liner (7); And be coated with the colored latex particle of tape label on the described fiberglass packing (2); Test wire (3) is set on the described chromatographic film (5), and described test wire (3) is that Bordetella pertussis toxin or Bordetella pertussis filamentous hemagglutinin are fixed on the upper formation of chromatographic film (5).
2. pertussis IgG antibody test card according to claim 1, it is characterized in that, control line (4) also is set on the described chromatographic film (5), and described test wire (3) is positioned between described sample pad (1) and the described control line (4).
3. pertussis IgG antibody test card according to claim 1 and 2 is characterized in that, the main body of described liner (7) is plastic mattress.
4. pertussis IgG antibody test card according to claim 3 is characterized in that, described sample pad (1) length is 20mm, and width is 5mm; Described fiberglass packing (2) length is 10mm, and width is 5mm; Described chromatographic film (5) length is 25mm, and width is 5mm; Described absorption pad (6) length is 20mm, and width is 5mm; Described liner (7) length is 30mm, and width is 5mm, and thickness is 1mm.
5. pertussis IgG antibody test card according to claim 3, it is characterized in that, described fiberglass packing (2) is for being coated with the fiberglass packing (2) with the colored latex particle of SPA mark, and described control line (4) is fixed on the upper control line that forms of chromatographic film (5) for SPA.
6. pertussis IgG antibody test card according to claim 3, it is characterized in that, described fiberglass packing (2) is for being coated with the fiberglass packing (2) with the colored latex particle of mouse-anti human IgG labeling of monoclonal antibody, and described control line (4) is fixed on the upper control line (4) that forms of chromatographic film (5) for sheep anti-mouse igg antibody.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220591890 CN202854142U (en) | 2012-11-09 | 2012-11-09 | Pertussis IgG antibody test card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220591890 CN202854142U (en) | 2012-11-09 | 2012-11-09 | Pertussis IgG antibody test card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202854142U true CN202854142U (en) | 2013-04-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220591890 Expired - Lifetime CN202854142U (en) | 2012-11-09 | 2012-11-09 | Pertussis IgG antibody test card |
Country Status (1)
| Country | Link |
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| CN (1) | CN202854142U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104198704A (en) * | 2014-09-02 | 2014-12-10 | 张明 | Pertussis diagnostic kit and preparation method thereof |
| CN108254556A (en) * | 2018-03-15 | 2018-07-06 | 北京科兴中维生物技术有限公司 | A kind of pertussis toxin detection kit and its application |
-
2012
- 2012-11-09 CN CN 201220591890 patent/CN202854142U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104198704A (en) * | 2014-09-02 | 2014-12-10 | 张明 | Pertussis diagnostic kit and preparation method thereof |
| CN104198704B (en) * | 2014-09-02 | 2015-11-11 | 张明 | A kind of pertussis diagnostic kit and preparation method |
| CN108254556A (en) * | 2018-03-15 | 2018-07-06 | 北京科兴中维生物技术有限公司 | A kind of pertussis toxin detection kit and its application |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20130403 |
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| CX01 | Expiry of patent term |