CN203965325U - Optical system before pond for integrated form fluorescence detector - Google Patents
Optical system before pond for integrated form fluorescence detector Download PDFInfo
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- CN203965325U CN203965325U CN201420293683.XU CN201420293683U CN203965325U CN 203965325 U CN203965325 U CN 203965325U CN 201420293683 U CN201420293683 U CN 201420293683U CN 203965325 U CN203965325 U CN 203965325U
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Abstract
The utility model provides optical system before pond for a kind of integrated form fluorescence detector, comprises the assemblies such as the first column, the second column and firmware.By simple technical scheme, utilize coaxial design and the processing of LED assembly, optical filter and collection optical fiber container structure, having realized LED chip and optical fiber receives between end face, still can concentric alignment across optical filter, thereby ensure larger excitation light intensity to import surveyed area, guarantee sensitivity; And described product structure is processed and can be realized by standard machinery.Also be easy to use and overhaul.
Description
Technical field
The present invention relates to fluorescence detector assembly, particularly a kind of light source coupling assembly based on light emitting diode (Light Emitting Diode, LED) induced fluorescent tester.
Background technology
Fluoroscopic examination is one of the highest detection technique of current sensitivity, has very high selectivity and the stability of response simultaneously, is widely used in recent years at trace biology material and environmental contaminants context of detection.Excitation light source system is the critical component in fluorescence detection device.As gas-discharge lamp and laser etc., all there is the shortcomings such as volume is large, energy consumption is high, the life-span is short in traditional light source.The progress of LED manufacturing process in recent years, becomes the novel excitation source of fluoroscopic examination, thereby has promoted the microminiaturization of instrument entirety, and keeps the detecting device of practical sensitivity and small-sized even stock size suitable.
But, with respect to laser equal excitation light source, there is following shortcoming in LED: (1) Gaussian distribution formula excitation band, the fluorescence spectrum of long LED bands of a spectrum tail end and dye molecule of extending has often produced to a certain degree overlapping, thereby brings higher noise and background level to integral fluorescence detecting device; (2) incoherent light source, its transmitting light beam is difficult to be focused onto the degree matching with capillary inner diameter or chip split tunnel, quite a few light does not directly enter in photoelectricity testing part through detection cell, causes higher background and noise level.For above problem, the measure of generally taking is at present: (1) selects suitable exciter filter, to the spectrum of demand can fully be seen through, the spectrum of lap can effectively be ended, and bands of a spectrum intersections (crosstalk) minimize; (2) will be after filtration the revised exciting light of mating plate, by fiber optic conduction to detection zone.For ensureing that optical fiber can conduct to detection zone by the exciting light of greater strength, require fiber end face and LED central point (being luminescence chip position) necessary coaxial.There is no at present commercialization assembly and can realize above-mentioned three's coaxial coupling and fix, need design badly and can meet by simple and mechanical processing the coupling device of above-mentioned requirements.
Summary of the invention
The purpose of this utility model is for the problems of the prior art, and a kind of simple solution light source, optical filter, the coaxial technical scheme of excitation fiber are provided.Optical system before pond for a kind of integrated form fluorescence detector described in the utility model, comprise: the first column, on one bottom surface, be coaxially provided with cylindric the first cell body, relatively on bottom surface, be in succession provided with cylindric first cavity and second cavity coaxial with the first column, described the first cell body is communicated with along axis by duct with the second cavity; First firmware in the tool hole, center matching with the first cavity; Second firmware in the tool hole, center matching with the second cavity; Pass successively the excitation fiber in the first Firmware Hub through hole, the second Firmware Hub through hole and duct;
Also comprise: the second column that external diameter and the first cell body internal diameter match, coaxially arranges cylindric the second cell body, storage circular filter in it on one bottom surface; Relatively on bottom surface, cylindric the 3rd cell body is coaxially set, storage round LED assembly in it; Described the second cell body and the 3rd cell body are communicated with; And,
The firmware that shape and the second column bottom surface match, which is provided with the passage as LED lead outlet.
The technical solution of the utility model is by simple technical scheme, utilize coaxial design and the processing of LED assembly, optical filter and collection optical fiber container structure, having realized LED chip and optical fiber receives between end face, still can concentric alignment across optical filter, thereby ensure larger excitation light intensity to import surveyed area, guarantee sensitivity; And described product structure is processed and can be realized by standard machinery.
Brief description of the drawings
By the description to embodiment of carrying out below in conjunction with accompanying drawing, above-mentioned and/or other object of the present invention and advantage will become apparent.Accompanying drawing 5 width of the present invention, wherein:
Fig. 1 is optical system discrete topology schematic diagram before pond for integrated form fluorescence detector described in the utility model;
Fig. 2 is optical system assembling structural representation before pond for integrated form fluorescence detector described in the utility model;
Fig. 3 is the A-A ' view of Fig. 2;
Fig. 4 is the B-B ' view of Fig. 2;
Fig. 5 is applied to fluorescence detector by integrated form fluorescence detector described in the utility model by optical system before pond, the electrophoretic image that analysis of fluorescence element obtains.
In figure: 110, the first column, 111, the first cell body, 112, the first cavity, 113, the second cavity, 114, duct, 120, the first firmware, 130, the second firmware, 140, excitation fiber; 210, the second column, 211, the second cell body, 212, the 3rd cell body, 213, screw hole I, 220, LED assembly, 230, optical filter; 310, firmware, 311, passage, 312, screw hole II, 320, screw.
Embodiment
Now will be in detail with reference to embodiments of the invention, the example of described embodiment is shown in the drawings, and wherein, identical label refers to identical parts all the time.Below will be by described embodiment is described with reference to accompanying drawing, to explain the present invention.
Embodiment 1. structures and assembling
Integrated form fluorescence detector comprises following assembly by optical system before pond:
1. the first column 110, on one bottom surface, be coaxially provided with cylindric the first cell body 111, relatively on bottom surface, be in succession provided with cylindric first cavity 112 and second cavity 113 coaxial with the first column 110, described the first cell body 111 is communicated with along axis by duct 114 with the second cavity 113;
The first described cell body 111 is provided with internal thread;
2. the first firmware 120, profile coordinates with the first cavity 112 compact shape, is provided with along axis the through hole that excitation fiber can be passed; The first firmware is provided with external thread, and the first cavity 112 is established the internal thread matching with it, and the two is threaded;
3. the second firmware 130, is made up of resilient material silica gel, and profile and the second cavity 113 form fit, be provided with along axis the through hole that excitation fiber can be passed; The second firmware 130 is highly slightly larger than the second cavity 113, thereby so that can there is the fixing excitation fiber 140 through axis through bore of elastic deformation while being pressed on the first firmware 120;
4. excitation fiber 140, under assembled state, excitation fiber 140 is successively through the first firmware 120 central through holes, the second firmware 130 central through holes and duct 114;
5. the second column 210, its external diameter and the first cell body 111 internal diameters match, and are coaxially provided for receiving cylindric second cell body 211 of optical filter on one side bottom surface; Relatively on bottom surface, be coaxially provided for receiving cylindric the 3rd cell body 212 of LED assembly 220; Described the second cell body 211 and the 3rd cell body 212 are communicated with;
The second described column 210 is provided with external thread, matches with the set internal thread of the first cell body 111;
On the bottom surface of the 3rd cell body 212 homonymies, be also arranged with 2 blind hole screw hole I 213;
6. LED assembly 220, circle, can compactness be accommodated in the 3rd cell body 212;
7. optical filter 230, circle, can compactness be accommodated in the second cell body 211;
8. firmware 310, for 220 encapsulation of LED assembly are fixed in the 3rd cell body 212, firmware 310 is the circle matching with the second column 210 bottom surfaces, which is provided with the passage 311 as LED lead outlet, and is provided with the through hole screw hole II 312 supporting with screw hole I 213; The caliber size of described passage 311 allows LED lead-in wire pass but be enough to packaged LED assembly;
9. screw 320, thus can be used in conjunction with and realize firmware 310 and be connected with the second column 210 with screw hole I 213, screw hole II 312.
Above-mentioned the first column 110, the second column 210 and firmware 310 are made by the PVC material of black.
Said modules is assembled by the following method:
First, excitation fiber 140 is passed to the first firmware 120 central through holes, the second firmware 130 central through holes and duct 114 successively, the second firmware 130 is positioned in the second cavity 113, adjusts excitation fiber 140 insertion depths its front end face is flushed with the first cell body 111 bottom surfaces; In the first cavity 112, screw in the first firmware 120, compress the second firmware 130 distortion by the second firmware 130 to realize the fixing of excitation fiber 140.
Then, LED assembly 220 is inserted to the 3rd cell body 212, LED goes between through the passage 311 on firmware 310; Utilize screw 320 with being threaded of screw hole II 312 and screw hole I 213, firmware 310 and the second column 210 to be linked together, thereby realize the fixing of LED assembly 220; And optical filter 230 is inserted to the second cell body 211, realize the assembling of LED assembly and optical filter.
Finally, the second column 210 after assembling is revolved as the first cell body 111, complete the entirety assembling of integrated form fluorescence detector optical system before pond.
The detection of embodiment 2. fluoresceins
Use the made integrated form fluorescence detector of above-described embodiment 1 optical system before pond to build fluorescence detector, adopt Capillary Electrophoresis mode analysis of fluorescence element:
Assembling scheme: adopt the LED luminescent device of centre wavelength 470nM, band flow-through interference filter BP470nm, the 5V constant pressure source 100 Ω resistance of contacting are LED power supply; Conduct exciting light by excitation fiber and be irradiated to detection zone.Carry out phosphor collection in the direction vertical with exciting light, through fiber optic conduction, eliminate background interference light by fluorescent optical filter (BP530nm), finally enter photomultiplier and be converted to electric signal and output to workstation.
Test condition: buffer solution: Na
2b
4o
7(10mM, pH8.0); Fused quartz kapillary: 365 μ m (external diameter) × 100 μ m (internal diameter) × 55/45cm (overall length/effectively); Separation voltage: 20kV.
Test result is as shown in Figure 5: getting signal to noise ratio (S/N ratio) is 3, and this detecting device detects and is limited to 1 × 10-9mol/L the concentration of fluorescein.
Claims (6)
1. an optical system before pond for integrated form fluorescence detector, comprising:
The first column (110), on one bottom surface, be coaxially provided with cylindric the first cell body (111), relatively on bottom surface, be in succession provided with cylindric first cavity (112) coaxial with the first column (110) and the second cavity (113), described the first cell body (111) is communicated with along axis by duct (114) with the second cavity (113);
First firmware (120) in the tool hole, center matching with the first cavity (112);
Second firmware (130) in the tool hole, center matching with the second cavity (113);
Pass successively the excitation fiber (140) of the first firmware (120) central through hole, the second firmware (130) central through hole and duct (114);
Also comprise:
The second column (210) that external diameter and the first cell body (111) internal diameter match, coaxially arranges cylindric the second cell body (211) on one bottom surface, storage circular filter (230) in it;
Relatively on bottom surface, cylindric the 3rd cell body (212) is coaxially set, storage round LED assembly (220) in it;
Described the second cell body (211) and the 3rd cell body (212) are communicated with;
And,
The firmware (310) that shape and the second column (210) bottom surface match, which is provided with the passage (311) as LED lead outlet.
2. optical system before pond for integrated form fluorescence detector according to claim 1, is characterized in that: described the first cell body (111) is provided with internal thread, and the second column (210) is provided with the external thread matching with it.
3. optical system before pond for integrated form fluorescence detector according to claim 1, is characterized in that: described the second right cylinder (210) and firmware (310) are removably connected by screw (320).
4. optical system before pond for integrated form fluorescence detector according to claim 1, is characterized in that: described the first column (110), the second column (210) and firmware (310) are made up of the PVC material of black.
5. optical system before pond for integrated form fluorescence detector according to claim 1, is characterized in that: described the second firmware (130) is made up of resilient material.
6. optical system before pond for integrated form fluorescence detector according to claim 5, is characterized in that: described the first firmware (120) is established external thread, and the first cavity (112) is established the internal thread matching with it.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420293683.XU CN203965325U (en) | 2014-06-04 | 2014-06-04 | Optical system before pond for integrated form fluorescence detector |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420293683.XU CN203965325U (en) | 2014-06-04 | 2014-06-04 | Optical system before pond for integrated form fluorescence detector |
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| Publication Number | Publication Date |
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| CN203965325U true CN203965325U (en) | 2014-11-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201420293683.XU Expired - Fee Related CN203965325U (en) | 2014-06-04 | 2014-06-04 | Optical system before pond for integrated form fluorescence detector |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110006859A (en) * | 2019-03-05 | 2019-07-12 | 北京雪迪龙科技股份有限公司 | A kind of fluorescent material detection device and the method for detecting fluorescent material |
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2014
- 2014-06-04 CN CN201420293683.XU patent/CN203965325U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110006859A (en) * | 2019-03-05 | 2019-07-12 | 北京雪迪龙科技股份有限公司 | A kind of fluorescent material detection device and the method for detecting fluorescent material |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141126 Termination date: 20210604 |