CN203745472U - Counter immunoelectrophoresis hole-forming device for detection of aleutian virus of mink - Google Patents
Counter immunoelectrophoresis hole-forming device for detection of aleutian virus of mink Download PDFInfo
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- CN203745472U CN203745472U CN201420152406.7U CN201420152406U CN203745472U CN 203745472 U CN203745472 U CN 203745472U CN 201420152406 U CN201420152406 U CN 201420152406U CN 203745472 U CN203745472 U CN 203745472U
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Abstract
The utility model provides a counter immunoelectrophoresis hole-forming device for detection of an aleutian virus of a mink. The counter immunoelectrophoresis hole-forming device comprises a rectangular forming bottom plate and a rectangular forming base, wherein fixing retaining sides are arranged on three edges of the forming base, and a movable retaining side is arranged on the other edge; a forming top plate is arranged right above the forming bottom plate; two grooves for mounting the forming bottom plate and the forming top plate are formed in the fixing retaining sides and the movable retaining side; a linear type anti-skid mat is arranged in each groove; an agar-agar forming cavity is formed between the forming top plate and the forming bottom plate; four groups of forming column mounting holes penetrating through the forming top plate are formed in the forming top plate, and each group includes two rows of forming column mounting holes; forming columns are arranged in the forming column mounting holes; the top ends of the forming columns are connected with one or more forming column fixing plates; an agar-agar addition hole is formed in the forming top plate. The counter immunoelectrophoresis hole-forming device has the advantages that the structure is simple, the use is convenient, prepared agar-agar plates are uniform in thickness, the degree of standardization is high, using amount of agar-agar is saved, the bottom sealing effect is good, naked fire is not applicable, and the test safety is high.
Description
Technical field
The utility model relates to virus detection techniques field, relates in particular to a kind of agar plate hole-forming device, specifically refers to that Aleutian Mink Disease Parvovirus detects the counter immunoelectrophoresis hole-forming device of use.
Background technology
Aleutian disease aleutian disease of mink claim again plasmacytosis (plasmacytosis), is take of infecting by AD poison that mink the causes chronic disease viral disease that lifelong toxaemia, whole body lymphopoiesis, serum r-globulin number increase, glomerulonephritis, arteries inflammation and hepatitis are feature.Having the genotypic mink of A Liushen even has 1/3 to die from this disease, Aleutian disease can make the nonpregnant rate of female ermine and young ermine mortality ratio significantly increase, the mating ability of public ermine declines, can also affect growth and make fur quality low, causing economic loss, is one of three large epidemic diseases of universally acknowledged foster ermine.
At present, in order to prevent the propagation of Aleutian disease in mink farming family, regularly mink to be carried out to the detection of AD poison, accomplish timely discovery, avoid the generation of epidemic situation.For the current common method of the detection of Aleutian Mink Disease Parvovirus, be that counter immunoelectrophoresis detects antiviral antibody, operation steps is as follows in detail:
Bed board: prepare the agar plate that thickness is 3mm on sheet glass plate;
Punching: then punch with punching model on the agar plate solidifying, general punching model is two hollow tubulars, by artificial hand-held punching model, on agar plate, punch, between Kong Yukong, pitch-row is that 4mm, aperture are 3mm in accordance with regulations, but Manual punching is difficult to accomplish strict unification, inadequate standard seems, after punching, to the agar in hole be chosen with pin, agar after choosing is wasted, if hole is beaten tiltedly, agar is difficult to choose, even if choose, also the integrality in hole may be destroyed, testing result will be affected;
Back cover: punch and complete glass plate is put to spirit lamp flame envelope heating, the gap between agar gel and glass plate between each hole is sealed, room temperature is cooling rear standby;
Then pass through application of sample, electrophoresis, carry out afterwards interpretation of result judgement.
In sum, current agar plate preparation process is complicated, loaded down with trivial details, and hand labor intensity is large, and the agar plate standardization level of preparation is low, also causes the waste of part agar, and back cover operation is used naked light, the defects such as risk factor height.
Summary of the invention
The technical problems to be solved in the utility model is for the existing weak point of prior art, provide a kind of simple in structure, easy to use, prepared agar plate even thickness, on agar plate, between each hole, gap is even, standardization level is high, save agar consumption, the effective and inapplicable naked light of back cover, the Aleutian Mink Disease Parvovirus that improves experimental safe coefficient detects the counter immunoelectrophoresis hole-forming device of use.
Technical solution of the present utility model is, provide following a kind of Aleutian Mink Disease Parvovirus to detect the counter immunoelectrophoresis hole-forming device of use, comprise forming bottom plate, described forming bottom plate is rectangle, also comprise shaping base, shaping base is rectangle, wherein three limits of shaping base are provided with fixedly rib, an other limit is provided with movable rib, directly over forming bottom plate, be provided with moulding top board, fixedly rib and movable rib are provided with two grooves of installation forming base plate and moulding top board, in groove, be provided with linear pattern slipmat, between moulding top board and forming bottom plate, be provided with agar forming cavity, the height of agar forming cavity is 3.0mm, moulding top board is provided with the four composing type post mounting holes that run through moulding top board, every group two row, in shaping column mounting hole, be provided with shaping column, shaping column top is connected to shaping column fixed head, moulding top board is provided with agar and adds hole.
Adopt above technical scheme, be convenient to the formation of agar plate, and the even thickness after agar plate forms, be of value to raising test accuracy, in addition, by shaping column is set, be convenient to the sample hole of the last moulding place test of agar plate use, thereby avoided the waste of agar, forming bottom plate and moulding top board and shaping base are groove type matching relationship, simple in structure, easy to use, in order to strengthen the steadiness of forming bottom plate and moulding top board, in the upper and lower surface of groove, straight line type is set, runs through the slipmat of whole recess sidewall, simple in structure, stablizing effect is good.
As preferably, described shaping column fixed head is one, two, three or four.Adopt the technical program, according to actual operation requirements, four composing type posts can be arranged on a formation of lots post fixed head, also can be arranged on two formation of lots post fixed heads, also can be arranged on three formation of lots post fixed heads, also can be arranged on four formation of lots post fixed heads, the size of shaping column fixed head is set according to actual service condition, usable range is more extensive.
As preferably, described shaping column fixed head both sides are provided with handle.Adopt the technical program, two handles are set, guaranteed stationarity when shaping column lifts, thereby guaranteed to test the regularity with sample hole on agar plate, bore edges is without phenomenons such as drawing cracks.
As preferably, described agar adds hole and is provided with funnel or is connected to pressure injector.Adopt the technical program, temperature is high, and the agar of good fluidity can be used funnel directly to pour into a mould, and the agar working pressure syringe slightly low for temperature, mobility is slightly poor is poured into a mould, and is all in order to guarantee the even thickness of agar plate, is convenient to later stage test.
As preferably, in described shaping base, be positioned at forming bottom plate below and be provided with resistive heater, shaping base is provided with power switch.Adopt the technical program, by Resistant heating, can avoid using naked light, reduced the danger coefficient in process of the test, security is more reliable.
As preferably, described shaping column is the stainless steel that material surface is smooth, and shaping column surfaceness is 0.025.Adopt the technical program, be convenient to shaping column and extract out from agar plate, and can not cause because friction force is excessive test to use the destruction in sample hole.
Adopt the beneficial effect of the technical program: prepared agar plate even thickness, be 3mm, on agar plate, between each hole, gap is even, and standardization level is high, saves agar consumption, the effective and inapplicable naked light of back cover, experimental safe coefficient is higher.
Accompanying drawing explanation
Fig. 1 is the structural representation of the utility model counter immunoelectrophoresis hole-forming device.
Fig. 2 is that Fig. 1 removes the structural representation after shaping column and moulding fixed head.
Fig. 3 is the structural representation after forming bottom plate, moulding top board and shaping column split.
Fig. 4 is counter immunoelectrophoresis hole-forming device partial cross section structural representation.
Shown in figure: 1, forming bottom plate, 2, shaping base, 3, fixing rib, 4, movable rib, 5, moulding top board, 6, groove, 7, linear pattern slipmat, 8, agar forming cavity, 9, shaping column mounting hole, 10, shaping column, 11, shaping column fixed head, 12, agar adds hole, 13, handle, 14, funnel, 15, resistive heater, 16, power switch.
Embodiment
For ease of explanation, below in conjunction with accompanying drawing, the counter immunoelectrophoresis hole-forming device of utility model is elaborated.
As shown in Figure 1 to Figure 4, a kind of Aleutian Mink Disease Parvovirus detects the counter immunoelectrophoresis hole-forming device of use, comprise forming bottom plate 1, described forming bottom plate 1 is rectangle, also comprise shaping base 2, shaping base 2 is rectangle, wherein three limits of shaping base 2 are provided with fixedly rib 3, an other limit is provided with movable rib 4, directly over forming bottom plate 1, be provided with moulding top board 5, fixedly rib 3 and movable rib 4 are provided with two grooves 6 of installation forming base plate 1 and moulding top board 5, in groove 6, be provided with linear pattern slipmat 7, between moulding top board 5 and forming bottom plate 1, be provided with agar forming cavity 8, the height of agar forming cavity 8 is 3.0mm, length is 100mm, width is 90mm, moulding top board 5 is provided with the four composing type post mounting holes 9 that run through moulding top board 5, every group two row, in shaping column mounting hole 9, be provided with shaping column 10, shaping column 10 tops are connected to shaping column fixed head 11, moulding top board 5 is provided with agar and adds hole 12, described shaping column fixed head 11 is four, described shaping column fixed head 11 both sides are provided with handle 13, described agar adds hole 12 and is provided with funnel 14, in described shaping base 2, be positioned at forming bottom plate 1 below and be provided with resistive heater 15, shaping base 2 is provided with power switch 16, described shaping column 10 is the stainless steel that material surface is smooth, and shaping column 10 surfacenesses are 0.025.
During use, first clean forming bottom plate 1 and moulding top board 5 are inserted in the groove 6 that shaping base 2 is provided with, then movable rib 4 is fastened, again the shaping column fixed head 11 with shaping column 10 is inserted in shaping column mounting hole 9, guarantee to fasten evenly, put in place, then the agar of heating and melting is injected in agar forming cavity 8 by funnel 14, wait to fill with by supercooling, last shaping column fixed head 11, shaping column 10 takes off together, while taking off, wanting both hands to hold handle 13 has together steadily vertically dragged, then turn on the power switch 16, resistive heater 15 is started working, the heat producing completes back cover, then unlatching activity rib 4, extract out and together extract forming bottom plate 1 and moulding top board 2 out, and then forming bottom plate 1 is separated with moulding top board 2, can obtain testing the agar plate of use.
Finally by crossing application of sample, electrophoretic action, check result is analyzed to judgement.
In the above-described embodiments, preferred forms of the present utility model is described, obviously, under inventive concept of the present utility model, still can make a lot of variations.At this, should illustrate, any change of making under inventive concept of the present utility model all will fall in protection domain of the present utility model.
Claims (6)
1. an Aleutian Mink Disease Parvovirus detects the counter immunoelectrophoresis hole-forming device of use, comprise forming bottom plate (1), it is characterized in that: described forming bottom plate (1) is rectangle, also comprise shaping base (2), shaping base (2) is rectangle, wherein three limits of shaping base (2) are provided with fixedly rib (3), an other limit is provided with movable rib (4), directly over forming bottom plate (1), be provided with moulding top board (5), fixedly rib (3) and movable rib (4) are provided with two grooves (6) of installation forming base plate (1) and moulding top board (5), in groove (6), be provided with linear pattern slipmat (7), between moulding top board (5) and forming bottom plate (1), be provided with agar forming cavity (8), the height of agar forming cavity (8) is 3.0mm, moulding top board (5) is provided with the four composing type post mounting holes (9) that run through moulding top board (5), every group two row, in shaping column mounting hole (9), be provided with shaping column (10), shaping column (10) top is connected to shaping column fixed head (11), moulding top board (5) is provided with agar and adds hole (12).
2. Aleutian Mink Disease Parvovirus according to claim 1 detects the counter immunoelectrophoresis hole-forming device of use, it is characterized in that: described shaping column fixed head (11) is one, two, three or four.
3. Aleutian Mink Disease Parvovirus according to claim 2 detects the counter immunoelectrophoresis hole-forming device of use, it is characterized in that: described shaping column fixed head (11) both sides are provided with handle (13).
4. Aleutian Mink Disease Parvovirus according to claim 1 detects the counter immunoelectrophoresis hole-forming device of use, it is characterized in that: described agar adds hole (12) and is provided with funnel (14) or is connected to pressure injector.
5. according to the Aleutian Mink Disease Parvovirus described in claim 3 or 4, detect the counter immunoelectrophoresis hole-forming device of use, it is characterized in that: in described shaping base (2), be positioned at forming bottom plate (1) below and be provided with resistive heater (15), shaping base (2) is provided with power switch (16).
6. Aleutian Mink Disease Parvovirus according to claim 5 detects the counter immunoelectrophoresis hole-forming device of use, it is characterized in that: described shaping column (10) is the smooth stainless steel of material surface, and shaping column (10) surfaceness is 0.025.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420152406.7U CN203745472U (en) | 2014-04-01 | 2014-04-01 | Counter immunoelectrophoresis hole-forming device for detection of aleutian virus of mink |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420152406.7U CN203745472U (en) | 2014-04-01 | 2014-04-01 | Counter immunoelectrophoresis hole-forming device for detection of aleutian virus of mink |
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| CN203745472U true CN203745472U (en) | 2014-07-30 |
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| CN201420152406.7U Expired - Fee Related CN203745472U (en) | 2014-04-01 | 2014-04-01 | Counter immunoelectrophoresis hole-forming device for detection of aleutian virus of mink |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107085115A (en) * | 2017-05-03 | 2017-08-22 | 中国农业科学院哈尔滨兽医研究所 | A kind of Aleutian Mink Disease Parvovirus double-antibody sandwich elisa antigen detection kit |
| CN110208531A (en) * | 2019-06-13 | 2019-09-06 | 河北科技师范学院 | A kind of scale Hu He ermine farm aleutian disease virus detection device device |
-
2014
- 2014-04-01 CN CN201420152406.7U patent/CN203745472U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107085115A (en) * | 2017-05-03 | 2017-08-22 | 中国农业科学院哈尔滨兽医研究所 | A kind of Aleutian Mink Disease Parvovirus double-antibody sandwich elisa antigen detection kit |
| CN107085115B (en) * | 2017-05-03 | 2018-11-16 | 中国农业科学院哈尔滨兽医研究所 | A kind of Aleutian Mink Disease Parvovirus double-antibody sandwich elisa antigen detection kit |
| CN110208531A (en) * | 2019-06-13 | 2019-09-06 | 河北科技师范学院 | A kind of scale Hu He ermine farm aleutian disease virus detection device device |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140730 Termination date: 20150401 |
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| EXPY | Termination of patent right or utility model |