CN203798772U - Sepharose gel making device - Google Patents
Sepharose gel making device Download PDFInfo
- Publication number
- CN203798772U CN203798772U CN201420206301.5U CN201420206301U CN203798772U CN 203798772 U CN203798772 U CN 203798772U CN 201420206301 U CN201420206301 U CN 201420206301U CN 203798772 U CN203798772 U CN 203798772U
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- China
- Prior art keywords
- comb
- gel
- plate
- glue
- groove
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Abstract
The utility model discloses a sepharose gel making device. The sepharose gel making device comprises a comb, a comb frame, a gel making board and a gel making trough, wherein the gel making trough is a square trough, the gel making board is a groove-shaped board with an upward opening and is placed in the gel making trough, the comb frame is a u-shaped frame and comprises connection rods on the two sides and a lapping rod in the middle, the lapping rod is in lap joint with the side wall of the gel making board, the ends, far away from the lapping rod, of the two connection rods are hinged to the two side walls of the gel making board, the inner sides of the two connection rods are respectively provided with a comb groove, the openings of the two comb grooves are opposite in position and extend vertically, the ends of the two sides of the comb board of the comb are detachably connected with the two comb grooves respectively, and comb teeth of the comb face downwards and are located in the gel making board. The sepharose gel making device guarantees that the comb is evenly pulled out after sepharose is solidified; in electrophoresis and staining processes, gel is taken out of electrophoresis fluid and staining fluid conveniently; the problem that the ends of the comb teeth make contact with the bottom of the gel making board to generate comb holes (also known as gel holes) to cause leakage is solved.
Description
Technical field
The utility model relates to the fields such as life science, veterinary science, Animal Science, especially a kind ofly detects the Ago-Gel preparation facilities that nucleic acid is used.
Background technology
Agarose gel electrophoresis is to using a kind of electrophoresis method of agarose as supporting dielectric.Ago-Gel has network structure, material molecule by time can be subject to the resistance of grid, larger molecule is subject to larger resistance when springing up, therefore in gel electrophoresis, character and the quantity of net charge is not only depended in the separation of charged particle, but also depending on molecular size, this has just improved resolution characteristic greatly.But because its aperture is quite large, concerning most protein, its molecular sieving effect is insignificant, is now widely used in the research of nucleic acid.
At present, have a lot of patents about Ago-Gel preparation facilities, what most of laboratories were used is the equipment of resin making that Liuyi Instruments Plant, Beijing produces.Although these equipment of resin making have its advantage separately, still there are some shortcomings in them, or can say and in user's position, still await improved place.
In glue process, there is following shortcoming in these devices:
After agarose solidifies, need to pull out comb, and in the process of operation easily because of the uneven glue hole of damaging firmly, the two ends of comb;
In electrophoresis and dyeing course, be difficult for gel to take out from electrophoresis liquid and dyeing liquor;
In addition, liquid gel is being poured into after glue plate, the comb of some equipment of resin making easily contacts with glue plate inner bottom surface, causes the problem of leaking under glue hole.
The deficiency existing for above gel preparation facilities, the utility model solved while pulling out comb firmly uneven, be difficult for taking out the problem of leaking under gel and glue hole from liquid
Utility model content
The utility model, for the deficiencies in the prior art, proposes Ago-Gel gel maker, guarantees that agarose solidifies the rear comb of extracting uniformly; In electrophoresis and dyeing course, instant gelatine takes out from electrophoresis liquid and dyeing liquor; Avoided broach end to contact the problem of leaking under the glue hole of causing with glue plate inner bottom surface.
In order to realize above-mentioned utility model object, the utility model provides following technical scheme:
Ago-Gel gel maker, comprises comb, comb chassis, glue plate and glue groove,
Described glue groove is square cell body, and described glue plate is trough plate, and described glue plate opening upwards is placed in described glue groove,
Described comb chassis is concave shape framework, comprises the bar of taking at the connecting link of both sides and middle part, described in take on the sidewall that bar rides over described glue plate,
It is side walls hinged with two of described glue plate respectively in two described connecting links end from taking bar far away described,
A comb groove is respectively installed in the inner side of two described connecting links, and two described comb groove notch positions vertically extend relatively and all,
The pecten both side ends of described comb respectively with two described detachable connections of comb groove, the broach of comb downwards, be positioned at described glue plate.
Further, the vertical bottom of described comb groove is provided with limiting plate,
The bottom of both side ends described pecten, that be positioned at described broach and the laminating of described limiting plate.
Further, on the two side of described glue plate one end, be separately installed with two articulated slabs, on described articulated slab, coupling shaft be installed,
End at two described connecting links of described comb chassis is provided with connecting hole, and two described connecting holes coordinate with two described coupling shafts respectively.
Further, above described connecting link, be provided with the baffle plate of vertical described connecting link and plate face level with corresponding position, described connecting hole position,
Described baffle plate one lateral edges aligns with the end edge that described connecting link is provided with described connecting hole.
Further, between the end of described broach and described glue plate inner bottom surface, leave gap.
Further, two described comb grooves be separately positioned on two described connecting links near described in take bar one end.
The utility model is fixed on comb in comb chassis, like this after agarose solidifies by draw comb chassis can extract uniformly comb, guarantee that comb hole is without prejudice; In electrophoresis and dyeing course, can from electrophoresis liquid and dyeing liquor, take out gel easily by the mode of hand-held comb chassis, it is 90 degree that the restriction of baffle plate makes the maximum anglec of rotation of comb chassis, this is convenient to utilize hand-held comb chassis to get glue; In addition, the bar of taking of comb chassis is taken on glue plate, and comb is fixed in comb chassis, and spacing broach and the glue plate inner bottom surface of making between comb chassis and glue plate has certain interval, thereby can not cause the problem of leaking under glue hole.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of Ago-Gel gel maker described in the utility model;
Fig. 2 is the schematic diagram of comb described in the utility model;
Fig. 3 is the schematic diagram of comb chassis described in the utility model;
Fig. 4 is the schematic diagram of glue plate described in the utility model;
Fig. 5 is the schematic diagram of glue groove described in the utility model.
Embodiment
Below in conjunction with accompanying drawing, the utility model is described in detail, the description of this part is only exemplary and explanatory, should not have any restriction to protection domain of the present utility model.
Ago-Gel gel maker as shown in Figures 1 to 5, comprises comb 1, comb chassis 2, glue plate 3 and glue groove 4.
Glue groove 4 is square cell body, and glue plate 3 is trough plate, and the outer bottom size of the base plate 34 of glue plate 3 and area are corresponding with glue groove 4 inner bottom surface sizes and area, and glue plate 3 opening upwards are placed in glue groove 4.
Comb chassis 2 is concave shape framework, by batten, is made, and what comprise the connecting link 21 of both sides and middle part takes bar 22, and the length of connecting link 21 is corresponding with the length of glue plate 3 sidewalls, takes on the sidewall that bar 22 rides over glue plate 3.The length of taking bar 22 in the present embodiment is 6.6 centimetres.
Hinged with two articulated slabs 32 of glue plate 3 respectively away from the end of taking bar 22 at two connecting links 21.
A comb groove 23 is respectively installed in the inner side of two connecting links 21, the notch position of two comb grooves 23 vertically extends relatively and all, groove 23 is detachable is connected with two combs respectively for pecten 11 both side ends of comb 1, the broach 12 of comb 1 downwards, be positioned at glue plate 3.
The comb vertical bottom of groove 23 is provided with limiting plate 231, the bottom of both side ends pecten 11, that be positioned at broach 12 and limiting plate 231 laminatings.Pecten 11 integral body can be made convex shape, and broach 12 is arranged on the narrower one end of pecten 11, and narrower both sides, one end inwardly concave formation shoulder, and comb groove 23 is inserted when interior in pecten 11 both sides, fit with limiting plate 231 in shoulder bottom.Between the end of broach 12 and glue plate 3 inner bottom surfaces, leave gap, the present embodiment intermediate gap size is 2 millimeters.
On the two side 31 of glue plate 3 one end, be separately installed with two articulated slabs 32, on two articulated slabs 32, coupling shaft 321 be all installed,
In the end of two connecting links 21 of comb chassis 2, being provided with 211, two connecting holes 211 of connecting hole coordinates with two coupling shafts 321 respectively.
Above connecting link 21, be provided with the baffle plate 24 of vertical connecting 21 and plate face level with connecting hole 211 corresponding positions, position,
Baffle plate 24 1 lateral edges align with the end edge that connecting link 21 is provided with connecting hole 211.When comb chassis 2 is when rotate itself and glue plate 3 hinged places, baffle plate 24 can gear on the articulated slab 32 of glue plate 3, making the maximum anglec of rotation of comb chassis 2 is 90 degree, gel landing from glue plate while avoiding comb chassis 2 rotation is unrestricted making to get glue.
Two comb grooves 23 are separately positioned on two connecting links 21 near the one end of taking bar 22.The utility model can split into comb 1, comb chassis 2, glue plate 3 and glue groove 4, to clean.
The utility model concrete operations scheme is as follows:
1. clean comb 1, comb chassis 2, glue plate 3 and glue groove 4, comb chassis 2 and glue plate 3 are assembled, glue plate 3 is put into glue groove 4, comb chassis keeps level, in the comb groove 23 that comb 1 is inserted in comb chassis 2.
2. the liquid Ago-Gel heating is poured in glue plate 3.
3. after Ago-Gel solidifies, above put forward comb chassis 2 comb 1 is extracted, take off comb 1, lift comb chassis 2 and take out glue plate 3 and solid gel, gel is put into electrophoretic buffer, loading, electrophoresis.
4. after electrophoresis finishes, lift comb chassis 2 and take out glue plate 3 and solid gel, put into dyeing liquor and dye.
5. after dyeing, lift comb chassis 2 and take out glue plate 3 and solid gel, gel is put into gel imaging system and observe electrophoresis detection result.
6. throw away the gel after detection, clean comb 1, comb chassis 2, glue plate 3 and glue groove 4, standby.
The utility model is fixed on comb in comb chassis, like this after agarose solidifies by draw comb chassis can extract uniformly comb, guarantee that comb hole is without prejudice; In electrophoresis and dyeing course, can from electrophoresis liquid and dyeing liquor, take out gel easily by the mode of hand-held comb chassis, it is 90 degree that the restriction of baffle plate makes the maximum anglec of rotation of comb chassis, this is convenient to hand-held comb chassis and gets glue; In addition, the bar of taking of comb chassis is taken on glue plate, and comb is fixed in comb chassis, and certain interval is arranged at spacing broach and the glue plate bottom of making between comb chassis and glue plate, thereby can not cause the problem of leaking under glue hole.
Claims (6)
1. Ago-Gel gel maker, is characterized in that, comprises comb, comb chassis, glue plate and glue groove,
Described glue groove is square cell body, and described glue plate is trough plate, and described glue plate opening upwards is placed in described glue groove,
Described comb chassis is concave shape framework, comprises the bar of taking at the connecting link of both sides and middle part, described in take on the sidewall that bar rides over described glue plate,
It is side walls hinged with two of described glue plate respectively in two described connecting links end from taking bar far away described,
A comb groove is respectively installed in the inner side of two described connecting links, and two described comb groove notch positions vertically extend relatively and all,
The pecten both side ends of described comb respectively with two described detachable connections of comb groove, the broach of comb downwards, be positioned at described glue plate.
2. Ago-Gel gel maker as claimed in claim 1, is characterized in that, the vertical bottom of described comb groove is provided with limiting plate,
The bottom of both side ends described pecten, that be positioned at described broach and the laminating of described limiting plate.
3. Ago-Gel gel maker as claimed in claim 1, is characterized in that, is separately installed with two articulated slabs on the two side of described glue plate one end, on described articulated slab, coupling shaft is installed,
End at two described connecting links of described comb chassis is provided with connecting hole, and two described connecting holes coordinate with two described coupling shafts respectively.
4. Ago-Gel gel maker as claimed in claim 3, is characterized in that, above described connecting link, be provided with the baffle plate of vertical described connecting link and plate face level with corresponding position, described connecting hole position,
Described baffle plate one lateral edges aligns with the end edge that described connecting link is provided with described connecting hole.
5. Ago-Gel gel maker as claimed in claim 1, is characterized in that, between the end of described broach and described glue plate inner bottom surface, leaves gap.
6. Ago-Gel gel maker as claimed in claim 1, is characterized in that, two described comb grooves be separately positioned on two described connecting links near described in take bar one end.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420206301.5U CN203798772U (en) | 2014-04-25 | 2014-04-25 | Sepharose gel making device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420206301.5U CN203798772U (en) | 2014-04-25 | 2014-04-25 | Sepharose gel making device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203798772U true CN203798772U (en) | 2014-08-27 |
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ID=51380977
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420206301.5U Expired - Fee Related CN203798772U (en) | 2014-04-25 | 2014-04-25 | Sepharose gel making device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203798772U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107831207A (en) * | 2017-12-07 | 2018-03-23 | 安徽师范大学 | A kind of glue utensil and its application method |
| CN109470760A (en) * | 2018-12-26 | 2019-03-15 | 天康生物股份有限公司 | It is used to prepare the device, agarose gel electrophoresis method for detecting and application of Ago-Gel |
| CN110161108A (en) * | 2018-03-30 | 2019-08-23 | 广安职业技术学院 | A kind of electrophoresis tank convenient for fixed plastic plate |
| CN111044594A (en) * | 2020-01-09 | 2020-04-21 | 温州医科大学附属第二医院、温州医科大学附属育英儿童医院 | Pulling-up device of electrophoresis comb |
| CN112213371A (en) * | 2019-07-12 | 2021-01-12 | 华大青兰生物科技(无锡)有限公司 | High-flux agarose gel electrophoresis device |
-
2014
- 2014-04-25 CN CN201420206301.5U patent/CN203798772U/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107831207A (en) * | 2017-12-07 | 2018-03-23 | 安徽师范大学 | A kind of glue utensil and its application method |
| CN107831207B (en) * | 2017-12-07 | 2024-03-26 | 安徽师范大学 | Glue making device and use method thereof |
| CN110161108A (en) * | 2018-03-30 | 2019-08-23 | 广安职业技术学院 | A kind of electrophoresis tank convenient for fixed plastic plate |
| CN109470760A (en) * | 2018-12-26 | 2019-03-15 | 天康生物股份有限公司 | It is used to prepare the device, agarose gel electrophoresis method for detecting and application of Ago-Gel |
| CN109470760B (en) * | 2018-12-26 | 2024-05-31 | 天康生物制药有限公司 | Device for preparing agarose gel, agarose gel electrophoresis method and application |
| CN112213371A (en) * | 2019-07-12 | 2021-01-12 | 华大青兰生物科技(无锡)有限公司 | High-flux agarose gel electrophoresis device |
| CN111044594A (en) * | 2020-01-09 | 2020-04-21 | 温州医科大学附属第二医院、温州医科大学附属育英儿童医院 | Pulling-up device of electrophoresis comb |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140827 Termination date: 20160425 |