CN203639119U

CN203639119U - Flat-ended polymerase chain reaction (PCR) tube cap compressing apparatus for laboratories

Info

Publication number: CN203639119U
Application number: CN201320791853.2U
Authority: CN
Inventors: 皮妍; 乔晓京; 董志强; 张丹; 段煜竹; 乔守怡; 蒋科技
Original assignee: Fudan University
Current assignee: Fudan University
Priority date: 2013-12-05
Filing date: 2013-12-05
Publication date: 2014-06-11
Anticipated expiration: 2023-12-05

Abstract

The utility model belongs to the technical field of bioexperiment, and particularly relates to a flat-ended polymerase chain reaction (PCR) tube cap compressing apparatus for laboratories. The flat-ended PCR tube cap compressing apparatus consists of a grip, a roller, a compressing ring and a compressing ring locking screw; one end of the grip is provided with a U-shaped groove with the roller fixed inside; the compressing ring is composed of a plurality of arc blocks which are spaced from one another and is arranged at the other end of the grip; the middle of the grip is cylindrical; the roller is of a cylindrical structure with the height smaller than the diameter of a PCR tube cap, and a plurality of circular ribs parallel to the cylindrical edge are arranged on the cambered surface of the cylindrical structure. The flat-ended PCR tube cap compressing apparatus can be used for compressing batched flat-ended PCR tube caps or individual flat-ended PCR tube cap, and can be widely applied to teaching and scientific research activities in biological laboratories, and especially laboratories needing PCR in scale. The flat-ended PCR tube cap compressing apparatus is easy to operate and capable of guaranteeing to compress all PCR tube caps in batch within a short time, and accordingly work efficiency is increased greatly.

Description

A kind of use for laboratory tack PCR pipe lid pinch device

Technical field

The utility model belongs to Bioexperiment technical field, is specifically related to a kind of use for laboratory tack PCR pipe lid pinch device.

Background technology

Round pcr is one of technology most important, the most frequently used in current biological study, and nearly all Molecular Biology Lab all needs to carry out PCR operation.In PCR operation process, the lid of PCR pipe is difficult to guarantee to cover tightly completely, and operator is not easy to discover and has the lid of which PCR pipe not cover tightly, in that case, because PCR reaction often needs to be heated to 94 degrees Celsius or higher temperature, if the lid of PCR pipe does not cover tightly, liquid in the process of PCR reaction in PCR pipe can be escaped because of evaporation, thereby change PCR reaction system, cause PCR to react unsuccessfully, even all liquid is all by evaporate to dryness.And, in the Bioexperiment teaching process of university, because student is numerous, or the sample of other scientific research missions is a lot, often need to carry out large batch of PCR reaction, in quantity size, be tens or often thousands of up to a hundred, whether experimenter covers tightly one by one and checks and examine the lid of each PCR pipe with energy with band religion teacher is not free.Therefore must there is a simple and practical instrument to address this problem.

Summary of the invention

The purpose of this utility model is to provide a kind of use for laboratory tack PCR pipe lid pinch device, is difficult to solve the problem of guaranteeing that PCR pipe lid all covers tightly in prior art.

The use for laboratory tack PCR pipe lid pinch device that the utility model provides, comprises handle, scroll wheel, extrusion ring and extrusion ring locking spiral pin; Wherein, described handle one end has U-shaped groove, and two arms of U-shaped groove are fixed at the two ends of the axle of described scroll wheel, makes scroll wheel flexible rotating in U-shaped groove; Described extrusion ring is arranged at the handle other end, and this extrusion ring is made up of some circular arc pieces of space; Described extrusion ring locking spiral pin, for locking each circular arc piece of extrusion ring, makes it relatively to fix; Described handle centre portion is cylindrical body, and cylindrical body is provided with some arc inner groovies at interval in the upper and lower, so that finger grip.

Described scroll wheel is cylindrical structural, and cylinder height is less than the diameter of PCR pipe lid, has some the ring-type crest lines parallel with cylindrical edge on the arcwall face of this cylinder, and crest line can evagination, also can indent.

Described extrusion ring is made up of 2 or multi-disc circular arc piece, and these circular arc pieces surround a complete annulus.

The circular arc piece of described extrusion ring can integral-rotation, and the space after rotation between circular arc piece becomes large or diminishes, thereby makes the space that circular arc piece comprises become large or diminish.

The diameter of the circular arc of described arc inner groovy on handle vertical direction conforms to adult's finger diameter or is bigger.

Described scroll wheel is made up of timber, rigid plastic or metal.

Described handle, extrusion ring and extrusion ring locking spiral pin are made up of plastics or metal.

Described handle is oval column type.

The using method of the use for laboratory tack PCR pipe lid pinch device that the utility model provides is as follows:

When school duty room has been collected student's great many of experiments PCR pipe, or scientific experiment chamber has completed the PCR response preparation of great amount of samples, it is the PCR pipe of plane for lid, PCR pipe (or the 12 PCR pipe of lid will be built, 96 PCR tube sheets etc.) fitly lie in a horizontal plane on PCR pipe flat board or on PCR instrument, operator grasps handle 1 of the present utility model, another hand steered firmly PCR pipe flat board or PCR instrument, make one end that handle 1 has a U-shaped groove down, aim at PCR pipe lid Shang center in a row with scroll wheel 2, from first initial PCR pipe lid, then make handle 1 vertical direction after 30 degree-an angle of 90 degrees, firmly press down handle 1, and along the slow mobile handle 1 of orientation of PCR pipe, annular rib on scroll wheel 2 can make the direction of scroll wheel be difficult for departing from, roll across successively the lid top of this row PCR pipe, the pressure of scroll wheel is covered tightly the lid of each PCR pipe being rolled across.

Single job can compress the lid of a row (as 12) PCR pipe, and then same operation until compress all PCR pipe lids, guarantees that in PCR reaction, reactant liquor can not escaped because of evaporation.Because adopted scroll wheel 2 surfaces to have the design of rib and handle shape handle 1, not only efficiency is higher with manual manipulation than pure, and apply moment, make to act on pure large with hand of force rate on pipe lid, easily make lid cover tightly, the design of the scroll wheel 2 upper ribs in surface also makes the sense of motion of scroll wheel be difficult for departing from.For the PCR pipe of proper alignment on PCR instrument, because the spacing in length and breadth all equates, also can take laterally to compress once, longitudinally compress and once carry out the dual effect of guaranteeing to compress.

For single PCR pipe lid fragmentary or scroll wheel malcompression, can adopt the extrusion ring of handle 1 other end to complete.When operation, first horizontal positioned PCR pipe to be operated is on PCR flat board or on PCR instrument, unclamp extrusion ring locking spiral pin 4, then forward and inverse hour hands rotary squeezing ring 3, regulate the distance between the each circular arc piece of extrusion ring 3, the diameter that makes it the annulus of its composition is less than the diameter of the PCR pipe lid of needs operation, then tightens extrusion ring locking spiral pin 4, the diameter of the distance between the each circular arc piece of fixed press ring 3 and the annulus of its composition.Operator holds handle 1 equally, and the one end that maintains extrusion ring 3 down, handle 1 is horizontal by 90 degree right angles, aim at the plane of PCR pipe lid to be operated with the annulus that extrusion ring 3 forms, and the center of extrusion ring 3 and the center of PCR pipe lid are coincided, hold handle 1 and firmly press down, can complete the operation of the compression lid to this PCR pipe.This operation presses the plane of PCR pipe lid laborsaving than press...withing one's finger merely.

Beneficial effect

The utility model can be used for PCR pipe in batch or single PCR pipe to compress operation, make the lid of PCR pipe cover compression completely, prevent that the liquid in PCR pipe is escaped because of evaporation in the process of PCR reaction, ensure the integraty of PCR reaction system, PCR reaction is carried out smoothly.The utility model can be widely used in teaching, the scientific research activity of biology laboratory, especially needs to carry out in enormous quantities the laboratory of PCR reaction.The utility model is simple to operate, efficient, after outfit, can guarantee that at short notice its lid all covers tightly to large batch of PCR pipe, has greatly improved work efficiency, and simultaneously laborsaving than pure manual manipulation, accuracy rate is high.

Brief description of the drawings

Fig. 1 is structural representation of the present utility model.

Number in the figure: 1-handle; 2-scroll wheel; 3-extrusion ring; 4-extrusion ring locking spiral pin; 5-arc inner groovy.

Detailed description of the invention

Below in conjunction with specific embodiment, further set forth the utility model.Should be understood that these embodiment are only not used in restriction scope of the present utility model for the utility model is described.In addition should be understood that those skilled in the art can make various changes or modifications the utility model after having read the content of the utility model instruction, these equivalent form of values fall within the application's appended claims limited range equally.

As shown in Figure 1, use for laboratory PCR pipe lid pinch device, comprise handle 1, scroll wheel 2, extrusion ring 3 and extrusion ring locking spiral pin 4, handle 1 one end has U-shaped groove, scroll wheel 2 is installed in groove, and handle 1 centre portion is distributed with some arc inner groovies 5, and handle 1 other end has extrusion ring 3, extrusion ring 3 is separated circular arc piece composition, and each circular arc piece that extrusion ring locking spiral pin 4 can be locked extrusion ring 3 makes it relatively to fix.

Described scroll wheel 2 is short cylindrical shape structure, and cylinder height is slightly less than the diameter of PCR pipe lid, has some the ring-type crest lines parallel with cylindrical edge on the arcwall face of this cylinder, and crest line can evagination, also can indent.

Described extrusion ring 3 is made up of 2-4 sheet circular arc piece, and these circular arc pieces surround a complete annulus.

The circular arc piece of described extrusion ring 3 can integral-rotation, and the space after rotation between circular arc piece becomes large or diminishes, thereby makes the space that circular arc piece comprises become large or diminish.

The diameter of circular arc of described arc indent rib 5 on handle 1 vertical direction and adult's finger diameter conform to or are bigger.

Described scroll wheel 2 is made up of timber, rigid plastic or metal etc.

Described handle 1, extrusion ring 3 and extrusion ring locking spiral pin 4 are made up of plastics or metal etc.

Described handle 1 is oval column type.

When school duty room has been collected student's great many of experiments PCR pipe, or scientific experiment chamber has completed the PCR response preparation of great amount of samples, it is the PCR pipe of plane for lid, PCR pipe (or the 12 PCR pipe of lid will be built, 96 PCR tube sheets etc.) fitly lie in a horizontal plane on PCR pipe flat board or on PCR instrument, operator grasps handle 1 of the present utility model, for ensureing to grip, finger can be embedded in the indent rib 5 on handle, another hand steered firmly PCR pipe flat board or PCR instrument, make one end that handle 1 has a U-shaped groove down, aim at PCR pipe lid Shang center in a row with scroll wheel 2, from first initial PCR pipe lid, then make handle 1 vertical direction after 30 degree-an angle of 90 degrees, firmly press down handle 1, keep necessarily pressing down dynamics, and along the slow mobile handle 1 of orientation of PCR pipe, annular rib on scroll wheel 2 can make the direction of scroll wheel be difficult for departing from, roll across successively the lid top of this row PCR pipe, the pressure of scroll wheel is covered tightly the lid of each PCR pipe being rolled across.Single job can compress the lid of a row (as 12) PCR pipe, and then same operation until compress all PCR pipe lids, guarantees that in PCR reaction, reactant liquor can not escaped because of evaporation.Because adopted scroll wheel 2 surfaces to have the design of rib and handle shape handle 1, not only efficiency is higher with manual manipulation than pure, and apply moment, make to act on pure large with hand of force rate on pipe lid, easily make lid cover tightly, the design of the scroll wheel 2 upper ribs in surface also makes the sense of motion of scroll wheel be difficult for departing from.For the PCR pipe of proper alignment on PCR instrument, because the spacing in length and breadth all equates, also can take laterally to compress once, longitudinally compress and once carry out the dual effect of guaranteeing to compress.

For the operation that similarly need to cover tightly pipe lid of other non-PCR reactions, as long as the structure of pipe and lid is applicable to, for example enzyme is lived and is reacted, and test chemical etc., all can apply the utility model.

Claims

1. a use for laboratory tack PCR pipe lid pinch device, is characterized in that being made up of handle, scroll wheel, extrusion ring and extrusion ring locking spiral pin; Wherein, described handle one end has U-shaped groove, and two arms of U-shaped groove are fixed at the two ends of the axle of described scroll wheel, makes scroll wheel flexible rotating in U-shaped groove; Described extrusion ring is arranged at the handle other end, and described extrusion ring is made up of some circular arc pieces of space, and described extrusion ring locking spiral pin, for locking each circular arc piece of extrusion ring, makes it relatively to fix; Described handle centre portion is cylindrical body, and cylindrical body is provided with some arc inner groovies at interval in the upper and lower, so that finger grip; Described scroll wheel is cylindrical structural, and cylinder height is less than the diameter of PCR pipe lid, has some the ring-type crest lines parallel with cylindrical edge on the arcwall face of this cylinder, and crest line is evagination, or is indent.

2. use for laboratory tack PCR pipe lid pinch device according to claim 1, is characterized in that, described extrusion ring is made up of 2-4 sheet circular arc piece, and these circular arc pieces surround a complete annulus.

3. use for laboratory tack PCR pipe lid pinch device according to claim 1, is characterized in that, the diameter of the circular arc of described arc inner groovy on handle vertical direction conforms to adult's finger diameter or be bigger.

4. use for laboratory tack PCR pipe lid pinch device according to claim 1, is characterized in that, described handle entirety is column type.