CN103663314A

CN103663314A - Flattop PCR tube cover pressing device used in laboratories and using method thereof

Info

Publication number: CN103663314A
Application number: CN201310645666.8A
Authority: CN
Inventors: 皮妍; 乔晓京; 董志强; 张丹; 段煜竹; 乔守怡; 蒋科技
Original assignee: Fudan University
Current assignee: Fudan University
Priority date: 2013-12-05
Filing date: 2013-12-05
Publication date: 2014-03-26
Anticipated expiration: 2033-12-05
Also published as: CN103663314B

Abstract

The invention belongs to the technical field of biological experiments, and in particular relates to a flat-head PCR tube cover pressing device for laboratories. The device is composed of a handle, a rolling wheel, an extrusion ring, and a locking screw pin of the extrusion ring; one end of the handle has a U-shaped groove, and the rolling wheel is fixed in the U-shaped groove; the extrusion ring is composed of several arcs spaced apart from each other. It consists of a block, which is arranged at the other end of the handle, and the middle part of the handle is a cylindrical body; the rolling wheel is a cylindrical structure, and the height of the cylinder is smaller than the diameter of the PCR tube cover. circular ridgeline. The invention can be used for pressing batches of flat-headed PCR tubes or single flat-headed PCR tubes, and can be widely used in teaching and scientific research activities in biological laboratories, especially laboratories that need to perform PCR reactions in large quantities. The invention is easy to operate, can ensure that the covers of a large number of flat-head PCR tubes are tightly closed in a short time, and greatly improves work efficiency.

Description

A kind of use for laboratory tack PCR pipe lid pinch device and using method thereof

Technical field

The invention belongs to Bioexperiment technical field, be specifically related to a kind of use for laboratory tack PCR pipe lid pinch device and using method thereof.

Background technology

Round pcr is one of technology most important, the most frequently used in current biological study, and nearly all Molecular Biology Lab all needs to carry out PCR operation.In PCR operation process, the lid of PCR pipe is difficult to guarantee to cover tightly completely, and operator is not easy to discover and has the lid of which PCR pipe not cover tightly, in that case, because PCR reaction often needs to be heated to 94 degrees Celsius or higher temperature, if the lid of PCR pipe does not cover tightly, liquid in the process of PCR reaction in PCR pipe can be escaped because of evaporation, thereby change PCR reaction system, cause PCR to react unsuccessfully, even all liquid is all by evaporate to dryness.And, in the Bioexperiment teaching process of university, because student is numerous, or the sample of other scientific research missions is a lot, often need to carry out large batch of PCR reaction, in quantity size, be tens or often thousands of up to a hundred, whether experimenter covers tightly one by one and checks and examine the lid of each PCR pipe with energy with band religion teacher is not free.Therefore must there is a simple and practical instrument to address this problem.

Summary of the invention

The object of the present invention is to provide a kind of use for laboratory tack PCR pipe lid pinch device and using method thereof, to solve, in prior art, be difficult to the problem of guaranteeing that PCR pipe lid all covers tightly.

Use for laboratory tack PCR pipe lid pinch device provided by the invention, comprises handle, scroll wheel, extrusion ring and extrusion ring locking spiral pin; Wherein, described handle one end has U-shaped groove, and two arms of U-shaped groove are fixed at the two ends of the axle of described scroll wheel, makes scroll wheel flexible rotating in U-shaped groove; Described extrusion ring is arranged at the handle other end, and this extrusion ring is comprised of some circular arc pieces of space; Described extrusion ring locking spiral pin, for locking each circular arc piece of extrusion ring, makes it relatively to fix; Described handle centre portion is cylindrical body, and cylindrical body is provided with some arc inner groovies at interval in the upper and lower, so that finger grip.

Described scroll wheel is cylindrical structural, and cylinder height is less than the diameter of PCR pipe lid, has some the ring-type crest lines parallel with cylindrical edge on the arcwall face of this cylinder, and crest line can evagination, also can indent.

Described extrusion ring is comprised of 2 or multi-disc circular arc piece, and these circular arc pieces surround a complete annulus.

The circular arc piece of described extrusion ring can integral-rotation, and the space after rotation between circular arc piece becomes large or diminishes, thereby makes the space that circular arc piece comprises become large or diminish.

The diameter of the circular arc of described arc inner groovy on handle vertical direction conforms to adult's finger diameter or is bigger.

Described scroll wheel is made by timber, rigid plastic or metal.

Described handle, extrusion ring and extrusion ring locking spiral pin are made by plastics or metal.

Described handle is oval column type.

The using method of use for laboratory PCR pipe lid pinch device provided by the invention is as follows:

When school duty room has been collected student's great many of experiments PCR pipe, or scientific experiment chamber has completed the PCR response preparation of great amount of samples, it for lid, is the PCR pipe of plane, PCR pipe (or the 12 PCR pipe of lid will be built, 96 PCR tube sheets etc.) fitly lying in a horizontal plane in PCR manages on above dull and stereotyped or PCR instrument, operator grasps handle 1 of the present invention, another hand steered PCR of living manages flat board or PCR instrument, make one end that handle 1 has a U-shaped groove down, with scroll wheel 2, aim at PCR pipe lid Shang center in a row, from first initial PCR pipe lid, then make handle 1 vertical direction after 30 degree-an angle of 90 degrees, firmly press down handle 1, and along the slow mobile handle 1 of orientation of PCR pipe, annular rib on scroll wheel 2 can make the direction of scroll wheel be difficult for departing from, roll across successively the lid top of this row PCR pipe, the pressure of scroll wheel is covered tightly the lid of each PCR pipe being rolled across.

Single job can compress the lid of a row (as 12) PCR pipe, and then same operation until compress all PCR pipe lids, guarantees that in PCR reaction, reactant liquor can not escaped because of evaporation.Because adopted scroll wheel 2 surfaces to have the design of rib and handle shape handle 1, not only efficiency is higher with manual manipulation than pure, and applied moment, make to act on pure large with hand of force rate on pipe lid, easily make lid cover tightly, the design of the scroll wheel 2 upper ribs in surface also makes the sense of motion of scroll wheel be difficult for departing from.For the PCR pipe of proper alignment on PCR instrument, because the spacing in length and breadth all equates, also can take laterally to compress once, longitudinally compress and once carry out the dual effect of guaranteeing to compress.

For single PCR pipe lid fragmentary or scroll wheel malcompression, can adopt the extrusion ring of handle 1 other end to complete.During operation, first horizontal positioned PCR pipe to be operated is on PCR flat board or on PCR instrument, unclamp extrusion ring locking spiral pin 4, then forward and inverse hour hands rotary squeezing ring 3, regulate the distance between extrusion ring 3 each circular arc pieces, the diameter that makes it the annulus of its composition is less than the diameter of the PCR pipe lid that needs operation, then tightens extrusion ring locking spiral pin 4, the diameter of the distance between fixed press ring 3 each circular arc pieces and the annulus of its composition.Operator holds handle 1 equally, and the one end that maintains extrusion ring 3 down, handle 1 is horizontal by 90 degree right angles, with the annulus that extrusion ring 3 forms, aim at the plane of PCR pipe lid to be operated, and the center of extrusion ring 3 and the center of PCR pipe lid are coincided, hold handle 1 and firmly press down, can complete the operation to the compression lid of this PCR pipe.This operation presses the plane of PCR pipe lid laborsaving than press...withing one's finger merely.

beneficial effect

The present invention can be used for PCR pipe in batch or single PCR pipe to compress operation, make the lid of PCR pipe cover compression completely, prevent that the liquid in PCR pipe is escaped because of evaporation in the process of PCR reaction, guarantee the integraty of PCR reaction system, PCR reaction is carried out smoothly.The present invention can be widely used in teaching, the scientific research activity of biology laboratory, especially needs to carry out in enormous quantities the laboratory of PCR reaction.The present invention is simple to operate, efficient, after outfit, can guarantee that at short notice its lid all covers tightly to large batch of PCR pipe, has greatly improved work efficiency, and simultaneously laborsaving than pure manual manipulation, accuracy rate is high.

Accompanying drawing explanation

Fig. 1 is structural representation of the present invention.

Number in the figure: 1-handle; 2-scroll wheel; 3-extrusion ring; 4-extrusion ring locking spiral pin; 5-arc inner groovy.

The specific embodiment

Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment are only not used in and limit the scope of the invention for the present invention is described.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read the content of the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.

As shown in Figure 1, use for laboratory tack PCR pipe lid pinch device, comprise handle 1, scroll wheel 2, extrusion ring 3 and extrusion ring locking spiral pin 4, handle 1 one end has U-shaped groove, scroll wheel 2 is installed in groove, and handle 1 centre portion is distributed with some arc inner groovies 5, and handle 1 other end has extrusion ring 3, extrusion ring 3 is that separated circular arc piece forms, and each circular arc piece that extrusion ring locking spiral pin 4 can be locked extrusion ring 3 makes it relatively to fix.

Described scroll wheel 2 is short cylindrical shape structure, and cylinder height is slightly less than the diameter of PCR pipe lid, has some the ring-type crest lines parallel with cylindrical edge on the arcwall face of this cylinder, and crest line can evagination, also can indent.

Described extrusion ring 3 is comprised of 2-4 sheet circular arc piece, and these circular arc pieces surround a complete annulus.

The circular arc piece of described extrusion ring 3 can integral-rotation, and the space after rotation between circular arc piece becomes large or diminishes, thereby makes the space that circular arc piece comprises become large or diminish.

The diameter of circular arc of described arc indent rib 5 on handle 1 vertical direction and adult's finger diameter conform to or are bigger.

Described scroll wheel 2 is made by timber, rigid plastic or metal etc.

Described handle 1, extrusion ring 3 and extrusion ring locking spiral pin 4 are made by plastics or metal etc.

Described handle 1 is oval column type.

When school duty room has been collected student's great many of experiments PCR pipe, or scientific experiment chamber has completed the PCR response preparation of great amount of samples, it for lid, is the PCR pipe of plane, PCR pipe (or the 12 PCR pipe of lid will be built, 96 PCR tube sheets etc.) fitly lying in a horizontal plane in PCR manages on above dull and stereotyped or PCR instrument, operator grasps handle 1 of the present invention, for guaranteeing to grip, finger can be embedded in the indent rib 5 on handle, another hand steered PCR of living manages flat board or PCR instrument, make one end that handle 1 has a U-shaped groove down, with scroll wheel 2, aim at PCR pipe lid Shang center in a row, from first initial PCR pipe lid, then make handle 1 vertical direction after 30 degree-an angle of 90 degrees, firmly press down handle 1, keep necessarily pressing down dynamics, and along the slow mobile handle 1 of orientation of PCR pipe, annular rib on scroll wheel 2 can make the direction of scroll wheel be difficult for departing from, roll across successively the lid top of this row PCR pipe, the pressure of scroll wheel is covered tightly the lid of each PCR pipe being rolled across.

Above-mentioned single job can compress the lid of a row (as 12) PCR pipe, and then same operation until compress all PCR pipe lids, guarantees that in PCR reaction, reactant liquor can not escaped because of evaporation.Because adopted scroll wheel 2 surfaces to have the design of rib and handle shape handle 1, not only efficiency is higher with manual manipulation than pure, and applied moment, make to act on pure large with hand of force rate on pipe lid, easily make lid cover tightly, the design of the scroll wheel 2 upper ribs in surface also makes the sense of motion of scroll wheel be difficult for departing from.For the PCR pipe of proper alignment on PCR instrument, because the spacing in length and breadth all equates, also can take laterally to compress once, longitudinally compress and once carry out the dual effect of guaranteeing to compress.

That for other non-PCR, reacts similarly need to cover tightly the operation of pipe lid, as long as the structure of pipe and lid is applicable to, for example enzyme is lived and reacted, and test chemical etc., all can apply the present invention.

Claims

1. The flat-headed PCR tube cover compression device for laboratories is characterized in that it is composed of a handle, a rolling wheel, an extrusion ring and an extrusion ring locking screw pin; wherein, one end of the handle has a U-shaped groove, and the The two ends of the shaft of the scroll wheel are fixed to the two arms of the U-shaped groove, so that the scroll wheel can rotate flexibly in the U-shaped groove; Composed of several arc blocks, the locking screw pins of the extrusion ring are used to lock each arc block of the extrusion ring to make them relatively fixed; the middle part of the handle is a cylindrical body, and the cylindrical body is provided with several The arc-shaped inner groove is convenient for fingers to hold; the rolling wheel is a cylindrical structure, and the height of the cylinder is less than the diameter of the PCR tube cover. The line is either convex or concave.

2. laboratory according to claim 1 flat head PCR tube cover compression device, it is characterized in that, described extrusion ring is made up of 2-4 arc blocks, and these arc blocks encircle a complete circle ring.

3. The laboratory flat-headed PCR tube cover compression device according to claim 1, wherein the diameter of the arc of the arc-shaped inner groove in the vertical direction of the grip is consistent with the diameter of the finger of an adult or slightly larger.

4. laboratory according to claim 1, is characterized in that, described handle is cylindrical as a whole.

5. a kind of using method of flat head PCR tube cover compression device as described in one of claim 1-4, it is characterized in that:

(1) For the PCR tube with a flat cover, place the PCR tube with the cover neatly and horizontally on the PCR tube plate or the PCR instrument, hold the handle with one hand, and hold the PCR tube plate with the other hand Or PCR machine, make the end of the handle with U-shaped groove face down, use the scroll wheel to align the center of the PCR tube cover in a row, start from the first PCR tube cover at the beginning, and then make the handle vertical After the direction is at an angle of 30°-90° to the horizontal plane, press down on the handle firmly, and slowly move the handle along the arrangement direction of PCR tubes. The circular edge on the scroll wheel makes it difficult to deviate from the direction of the scroll wheel, and roll over the row in turn. Above the lid of the PCR tube, the pressure of the rolling wheel makes the lid of each rolled PCR tube tightly closed;

(2) For sporadic or unpressed single PCR tube caps, use the squeeze ring at the other end of the handle to complete; when operating, first place the PCR tube to be operated horizontally on the PCR plate or on the PCR machine , loosen the locking screw pin of the extrusion ring, and then rotate the extrusion ring forward and counterclockwise to adjust the distance between the arc blocks of the extrusion ring so that the diameter of the ring formed by it is smaller than that of the PCR tube cap to be operated. diameter, and then tighten the extrusion ring locking screw pin to fix the distance between the arc blocks of the extrusion ring and the diameter of the ring formed; the operator holds the handle and keeps the end with the extrusion ring facing down, holding Align the ring formed by the extrusion ring at a right angle of 90 degrees to the horizontal plane with the plane of the PCR tube cover to be operated, and make the center of the extrusion ring coincide with the center of the PCR tube cover, hold the handle and press down firmly , that is, the operation of pressing the lid of the PCR tube is completed.