CN103663314B - A kind of use for laboratory tack PCR pipe lid pinch device and using method thereof - Google Patents

Abstract

The invention belongs to the technical field of biological experiments, and in particular relates to a flat-head PCR tube cover pressing device for laboratories. The device is composed of a handle, a rolling wheel, an extrusion ring, and a locking screw pin of the extrusion ring; one end of the handle has a U-shaped groove, and the rolling wheel is fixed in the U-shaped groove; the extrusion ring is composed of several arcs spaced apart from each other. It consists of a block, which is arranged at the other end of the handle, and the middle part of the handle is a cylindrical body; the rolling wheel is a cylindrical structure, and the height of the cylinder is smaller than the diameter of the PCR tube cover. circular ridgeline. The invention can be used for pressing batches of flat-headed PCR tubes or single flat-headed PCR tubes, and can be widely used in teaching and scientific research activities in biological laboratories, especially laboratories that need to perform PCR reactions in large quantities. The invention is easy to operate, can ensure that the covers of a large number of flat-head PCR tubes are tightly closed in a short time, and greatly improves work efficiency.

Description

A flat-head PCR tube cover compression device for laboratory use and its application method

technical field

The invention belongs to the technical field of biological experiments, and in particular relates to a flat-head PCR tube cover pressing device for a laboratory and an application method thereof. the

Background technique

PCR technology is one of the most important and commonly used technologies in biological research today, and almost all molecular biology laboratories need to perform PCR reaction operations. During the PCR reaction operation, it is difficult to ensure that the caps of the PCR tubes are completely closed, and it is not easy for the operator to detect which PCR tubes are not closed tightly. In that case, because the PCR reaction often needs to be heated to 94 degrees Celsius Or at a higher temperature, if the cap of the PCR tube is not tightly closed, the liquid in the PCR tube will escape due to evaporation during the PCR reaction, thereby changing the PCR reaction system, causing the PCR reaction to fail, and even all the liquid is evaporated to dryness. Moreover, in the process of biological experiment teaching in universities, due to the large number of students or the large number of samples for other scientific research tasks, it is often necessary to carry out a large number of PCR reactions. The teacher does not have the time and energy to check and verify whether the lid of each PCR tube is tightly closed. Therefore, there must be a simple and practical tool to solve this problem.

Contents of the invention

The object of the present invention is to provide a laboratory flat head PCR tube cover pressing device and its use method to solve the problem in the prior art that it is difficult to ensure that all PCR tube covers are closed tightly.

The flat-headed PCR tube cover compression device provided by the present invention includes a handle, a rolling wheel, an extrusion ring and an extrusion ring locking screw pin; wherein, one end of the handle has a U-shaped groove, and the rolling wheel The two ends of the shaft are fixed to the two arms of the U-shaped groove, so that the scroll wheel can rotate flexibly in the U-shaped groove; block; the locking screw pin of the extrusion ring is used to lock each arc block of the extrusion ring to make it relatively fixed; the middle part of the handle is a cylindrical body, and the cylindrical body is provided with a number of arc inner Grooves for finger grip.

The rolling wheel is a cylindrical structure, the height of which is smaller than the diameter of the PCR tube cover, and the arc surface of the cylinder has several circular ridges parallel to the sides of the cylinder, and the ridges can be convex or concave.

The extrusion ring is composed of two or more arc blocks, and these arc blocks form a complete ring.

The arc blocks of the extrusion ring can be rotated as a whole, and after rotation, the gaps between the arc blocks become larger or smaller, so that the space contained in the arc blocks becomes larger or smaller.

The diameter of the circular arc of the arc-shaped inner groove in the vertical direction of the handle is consistent with or slightly larger than the diameter of an adult's finger.

Described rolling wheel is made of wood, hard plastics or metal.

The handle, the squeeze ring and the squeeze ring locking screw are made of plastic or metal.

The handle is long cylindrical.

The using method of the laboratory PCR tube cover compression device provided by the invention is as follows:

When the teaching laboratory has collected a large number of experimental PCR tubes from students, or the scientific research laboratory has completed the PCR reaction preparation of a large number of samples, for the PCR tubes with flat covers, the PCR tubes with the covers (or 12-section PCR tubes, 96 PCR tube plate, etc.) neatly and horizontally placed on the PCR tube plate or PCR instrument, the operator holds the handle 1 of the present invention with one hand, and holds the PCR tube plate or PCR instrument with the other hand, so that the handle 1 has One end of the U-shaped groove is facing down, and use the scroll wheel 2 to align the center of the PCR tube cover in a row, starting from the first PCR tube cover at the beginning, and then make the vertical direction of the handle 1 be 30 degrees to the horizontal plane After the -90 degree angle, press the handle 1 firmly, and slowly move the handle 1 along the direction of the PCR tube arrangement. The circular edge on the rolling wheel 2 can make the direction of the rolling wheel not easy to deviate, and roll over the row of PCR tubes in turn. Above the cap, the pressure of the rolling wheel makes the cap of each rolled PCR tube tight.

One operation can compress the caps of a row (such as 12) of PCR tubes, and then do the same until the caps of all PCR tubes are compressed to ensure that the reaction solution will not escape due to evaporation during the PCR reaction. Because the surface of the rolling wheel 2 is ribbed and the design of the handle 1 is adopted, not only the efficiency is higher than that of pure hand operation, but also the torque is applied, so that the force acting on the tube cover is larger than that of pure hand operation, and it is easy to make the cover The cover is tight, and the design of the ribs on the surface of the scroll wheel 2 also makes the movement direction of the scroll wheel difficult to deviate from. For the PCR tubes neatly arranged on the PCR instrument, because the vertical and horizontal spacing are equal, it is also possible to compress once horizontally and once vertically to double ensure the compacting effect.

For sporadic or uncompressed individual PCR tube caps, the squeeze ring at the other end of the handle 1 can be used to complete. When operating, place the PCR tube to be operated horizontally on the PCR plate or PCR instrument, loosen the locking screw pin 4 of the extrusion ring, and then rotate the extrusion ring 3 forward and counterclockwise to adjust the arc blocks of the extrusion ring 3 distance between them, so that the diameter of the circular ring it forms is smaller than the diameter of the PCR tube cover that needs to be operated, and then tighten the extrusion ring locking screw pin 4 to fix the distance between each arc block of the extrusion ring 3 and its composition. The diameter of the ring. The operator also holds the handle 1, and keeps the end with the squeeze ring 3 facing down. The handle 1 is at a 90-degree right angle to the horizontal plane, and the ring formed by the squeeze ring 3 is aligned with the plane of the PCR tube cover to be operated. , and make the center of the extruding ring 3 coincide with the center of the PCR tube cover, hold the handle 1 and press down firmly to complete the operation of compressing the cover of the PCR tube. This operation is less labor-intensive than simply pressing the flat surface of the PCR tube cover with your fingers.

Beneficial effect

The present invention can be used for compressing batches of PCR tubes or individual PCR tubes, so that the covers of the PCR tubes are completely covered and compacted, preventing the liquid in the PCR tubes from escaping due to evaporation during the PCR reaction, and ensuring the PCR The integrity of the reaction system enables the PCR reaction to proceed smoothly. The invention can be widely used in teaching and scientific research activities in biological laboratories, especially in laboratories that need to perform PCR reactions in large quantities. The invention is simple and efficient in operation, and after being equipped, the caps of a large number of PCR tubes can be tightly closed in a short time, greatly improving the work efficiency, and at the same time, it saves labor and has high precision compared with pure manual operation.

Description of drawings

Fig. 1 is a structural schematic diagram of the present invention.

Symbols in the figure: 1-grip; 2-rolling wheel; 3-extrusion ring; 4-extrusion ring locking screw pin; 5-arc inner groove.

Detailed ways

Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the teachings of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

As shown in Figure 1, the flat-headed PCR tube cover compression device for laboratories includes a handle 1, a rolling wheel 2, an extrusion ring 3 and an extrusion ring locking screw pin 4, and one end of the handle 1 has a U-shaped groove, which is concave. A rolling wheel 2 is installed in the groove, a number of arc-shaped inner grooves 5 are distributed in the middle part of the handle 1, and an extrusion ring 3 is provided at the other end of the handle 1, and the extrusion ring 3 is composed of separated arc blocks. The ring locking screw pin 4 can lock each arc block of the extrusion ring 3 to make it relatively fixed.

The rolling wheel 2 is a short cylindrical structure, and the height of the cylinder is slightly smaller than the diameter of the PCR tube cover. The arc surface of the cylinder has several circular ridges parallel to the sides of the cylinder, and the ridges can be convex or inward. concave.

The extrusion ring 3 is composed of 2-4 arc blocks, and these arc blocks form a complete ring.

The arc blocks of the extrusion ring 3 can be rotated as a whole, and the gaps between the arc blocks become larger or smaller after rotation, so that the space contained in the arc blocks becomes larger or smaller.

The diameter of the circular arc in the vertical direction of the grip 1 of the arc-shaped inner concave edge 5 is consistent with or slightly larger than the diameter of an adult's finger.

Described rolling wheel 2 is made of wood, hard plastic or metal etc.

The handle 1, the extrusion ring 3 and the extrusion ring locking screw pin 4 are made of plastic or metal.

The handle 1 is long cylindrical.

When the teaching laboratory has collected a large number of experimental PCR tubes from students, or the scientific research laboratory has completed the PCR reaction preparation of a large number of samples, for the PCR tubes with flat covers, the PCR tubes with the covers (or 12-section PCR tubes, 96 PCR tube plate, etc.) neatly and horizontally placed on the PCR tube plate or on the PCR instrument, the operator holds the handle 1 of the present invention with his hand, and in order to ensure a firm grip, fingers can be embedded in the concave edge 5 on the handle Hold the PCR tube plate or PCR instrument with the other hand, make the end of the grip 1 with the U-shaped groove face down, and use the rolling wheel 2 to align the center of the PCR tube covers in a row, starting from the first Start with a PCR tube cover, and then make the vertical direction of the handle 1 form an angle of 30-90 degrees with the horizontal plane, press down on the handle 1 firmly, maintain a certain pressure, and slowly move the handle along the direction of the PCR tube arrangement 1. The annular edge on the rolling wheel 2 can make the direction of the rolling wheel not easy to deviate, and roll over the top of the cover of the row of PCR tubes in turn, and the pressure of the rolling wheel makes the cover of each rolled PCR tube tightly closed.

The above operation can compress the caps of a row (such as 12) of PCR tubes, and then perform the same operation until all the PCR tube caps are compressed to ensure that the reaction solution will not escape due to evaporation during the PCR reaction. Because the surface of the rolling wheel 2 is ribbed and the design of the handle 1 is adopted, not only the efficiency is higher than that of pure hand operation, but also the torque is applied, so that the force acting on the tube cover is larger than that of pure hand operation, and it is easy to make the cover The cover is tight, and the design of the ribs on the surface of the scroll wheel 2 also makes the movement direction of the scroll wheel difficult to deviate from. For the PCR tubes neatly arranged on the PCR instrument, because the vertical and horizontal spacing are equal, it is also possible to compress once horizontally and once vertically to double ensure the compacting effect.

For sporadic or uncompressed individual PCR tube caps, the squeeze ring at the other end of the handle 1 can be used to complete. When operating, place the PCR tube to be operated horizontally on the PCR plate or PCR instrument, loosen the locking screw pin 4 of the extrusion ring, and then rotate the extrusion ring 3 forward and counterclockwise to adjust the arc blocks of the extrusion ring 3 distance between them, so that the diameter of the circular ring it forms is smaller than the diameter of the PCR tube cover that needs to be operated, and then tighten the extrusion ring locking screw pin 4 to fix the distance between each arc block of the extrusion ring 3 and its composition. The diameter of the ring. The operator also holds the handle 1, and keeps the end with the squeeze ring 3 facing down. The handle 1 is at a 90-degree right angle to the horizontal plane, and the ring formed by the squeeze ring 3 is aligned with the plane of the PCR tube cover to be operated. , and make the center of the extruding ring 3 coincide with the center of the PCR tube cover, hold the handle 1 and press down firmly to complete the operation of compressing the cover of the PCR tube. This operation is less labor-intensive than simply pressing the flat surface of the PCR tube cover with your fingers.

For other non-PCR reactions, similar operations that need to tightly close the cap of the tube, as long as the structure of the tube and the cap is suitable, such as enzyme activity reaction, chemical test, etc., the present invention can be applied.

Claims (5)

1. use for laboratory tack PCR pipe lid pinch device, is characterized in that locking spiral pin by handle, scroll wheel, extrusion ring and extrusion ring forms; Wherein, described handle one end has U-shaped groove, and the two-arm of U-shaped groove is fixed at the two ends of the axle of described scroll wheel, makes scroll wheel flexible rotating in U-shaped groove; Described extrusion ring is arranged at the handle other end, and described extrusion ring is made up of spaced some circular arc blocks, and described extrusion ring locking spiral pin, for locking each circular arc block of extrusion ring, makes it relatively to fix; Described handle centre portion is cylindrical body, and cylindrical body is provided with some arc concave shape grooves at interval in the upper and lower, so that finger grip; Described scroll wheel is cylindrical structural, and cylinder height is less than the diameter of the sub-lid of PCR pipe, and the arcwall face of this cylinder has some ring-type crest lines parallel with cylindrical edge, crest line is evagination, or is indent.

2. use for laboratory tack PCR pipe lid pinch device according to claim 1, it is characterized in that, described extrusion ring is made up of 2-4 sheet circular arc block, and these circular arc blocks surround a complete annulus.

3. use for laboratory tack PCR pipe lid pinch device according to claim 1, is characterized in that, the diameter of the circular arc of described arc concave shape groove on handle vertical direction conforms to the finger diameter of adult or bigger.

4. use for laboratory tack PCR pipe lid pinch device according to claim 1, is characterized in that, described handle entirety is column type.

5. a using method for the use for laboratory tack PCR pipe lid pinch device as described in one of claim 1-4, is characterized in that:

(1) for lid be plane PCR pipe son, the PCR pipe building lid is fitly lain in a horizontal plane on PCR pipe flat board or in PCR instrument, operator grasps handle, another hand steered firmly PCR pipe flat board or PCR instrument, handle is made to have one end of U-shaped groove down, the center on PCR pipe lid in a row is aimed at scroll wheel, from initial first PCR pipe lid, then make handle vertical direction after 30 degree-an angle of 90 degrees, firmly press down handle, and along the slow mobile handle of orientation of PCR pipe, annular rib on scroll wheel makes the direction of scroll wheel not easily depart from, above the lid rolling across this row's PCR pipe successively, the pressure of scroll wheel makes each lid by PCR pipe rolled across be covered tightly,

(2) for the sub-lid of single PCR pipe that is fragmentary or scroll wheel malcompression, the extrusion ring of the handle other end is adopted; During operation, first horizontal positioned PCR pipe to be operated is on PCR flat board or in PCR instrument, unclamp extrusion ring locking spiral pin, then forward and inverse hour hands rotary squeezing ring, regulate the distance between each circular arc block of extrusion ring, the diameter making it the annulus of its composition is less than the diameter of the sub-lid of PCR pipe of needs operation, then tightens extrusion ring locking spiral pin, the diameter of the distance between each circular arc block of fixed press ring and the annulus of its composition; Operator holds handle, and the one end maintaining extrusion ring down, handle is horizontal by 90 degree of right angles, the plane of PCR pipe lid to be operated is aimed at the annulus that extrusion ring is formed, and the center of extruding Ring current distribution and PCR pipe lid is coincided, hold handle firmly to press down, namely complete the operation of the compression lid to this PCR pipe.