CN103641049B - A kind of use for laboratory round end PCR pipe lid pinch device and using method thereof - Google Patents
A kind of use for laboratory round end PCR pipe lid pinch device and using method thereof Download PDFInfo
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- CN103641049B CN103641049B CN201310645637.1A CN201310645637A CN103641049B CN 103641049 B CN103641049 B CN 103641049B CN 201310645637 A CN201310645637 A CN 201310645637A CN 103641049 B CN103641049 B CN 103641049B
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- pcr pipe
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- extrusion ring
- pcr
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Abstract
The invention belongs to field of bioexperiment technology, be specifically related to a kind of use for laboratory round end PCR pipe lid pinch device.This device is locked spiral pin formed by handle, scroll wheel, extrusion ring, extrusion ring; Handle one end has U-shaped groove, and scroll wheel is fixed in U-shaped groove; Extrusion ring is made up of spaced some circular arc blocks, is arranged at the handle other end, and handle centre portion is cylindrical body; Described scroll wheel is double-deck disk connection structure, the circular protrusions tabling on the void shape between two disks and the sub-lid of PCR pipe.The present invention can be used for carrying out compression operation to round end PCR pipe in batch or single PCR pipe, can be widely used in the teaching of biology laboratory, scientific research activity, especially need the laboratory of carrying out PCR reaction in enormous quantities.The present invention is simple to operate, can guarantee that its lid all covers tightly at short notice, greatly increase work efficiency to large batch of PCR pipe.
Description
Technical field
The invention belongs to field of bioexperiment technology, be specifically related to a kind of use for laboratory round end PCR pipe lid pinch device and using method thereof.
Background technology
Round pcr is one of technology most important, the most frequently used in current biological study, and nearly all Molecular Biology Lab all needs to carry out PCR operation.In PCR operation process, the lid of PCR pipe is difficult to guarantee to cover tightly completely, and operator is not easy to discover and has the lid of which PCR pipe not cover tightly, in that case, because PCR reaction often needs to be heated to 94 degrees Celsius or higher temperature, if the lid of PCR pipe does not cover tightly, liquid in the process of PCR reaction in PCR pipe can be escaped because of evaporation, thus change PCR reaction system, cause PCR to react unsuccessfully, even all liquid is all by evaporate to dryness.And, in the Bioexperiment teaching process of university, because student is numerous, or the sample of other scientific research missions is a lot, often need to carry out large batch of PCR reaction, quantity size is tens or frequent thousands of up to a hundred, whether experimenter and clinical tutor have no time to cover tightly the lid of each PCR pipe with energy to check one by one and examine.Therefore a simple and practical instrument must be had to address this problem.
Summary of the invention
The object of the present invention is to provide a kind of use for laboratory round end PCR pipe lid pinch device and using method thereof, to solve in prior art the problem being difficult to guarantee that the sub-lid of PCR pipe all covers tightly.
Use for laboratory round end PCR pipe lid pinch device provided by the invention, comprises handle, scroll wheel, extrusion ring and extrusion ring locking spiral pin; Wherein, described handle one end has U-shaped groove, and the two-arm of U-shaped groove is fixed at the two ends of the axle of described scroll wheel, makes scroll wheel flexible rotating in U-shaped groove; Described extrusion ring is arranged at the handle other end, and this extrusion ring is made up of spaced some circular arc blocks; Described extrusion ring locking spiral pin, for locking each circular arc block of extrusion ring, makes it relatively to fix; Described handle centre portion is cylindrical body, and cylindrical body is provided with some arc concave shape grooves at interval in the upper and lower, so that finger grip.
Described scroll wheel is double-deck disk connection structure, circular protrusions tabling on the sub-lid of PCR pipe of the void shape between two disks and 0.2ml, also namely in this structure by the arc curve tabling of the projection on the indent arc curve of section at the center of two disks and the sub-lid of PCR pipe of 0.2ml.
Described extrusion ring is 2 or multi-disc circular arc block, and it can form complete annulus.
The circular arc block of described extrusion ring can integral-rotation, and the space after rotating between circular arc block becomes large or diminishes, thus the space that circular arc block is comprised becomes large or diminishes.
The diameter of the circular arc of described arc concave shape groove on handle vertical direction conforms to the finger diameter of adult or bigger.
Described scroll wheel is made up of timber, rigid plastic or metal.
Described handle, extrusion ring and extrusion ring locking spiral pin is made up of plastics or metal.
Described handle is oval column type.
The using method of use for laboratory round end PCR pipe lid pinch device provided by the invention is as follows:
For PCR pipe lid with round end projection, to build PCR pipe (or 12 PCR pipe of lid, 96 PCR pipe plates etc.) fitly lie in a horizontal plane on PCR pipe flat board or in PCR instrument, operator grasps handle 1 of the present invention, another hand steered firmly PCR pipe flat board or PCR instrument, handle 1 is made to have one end of U-shaped groove down, the round end aimed on PCR pipe lid in a row with the gap between two disks of scroll wheel 2 is protruding, make the gap between protruding two disks imbedding scroll wheel 2 of the round end on first initial PCR pipe lid, then make handle 1 vertical direction after 30 degree-an angle of 90 degrees, firmly press down handle 1, and along the slow mobile handle 1 of orientation of PCR pipe, scroll wheel 2 is made to roll across above the lid of this row's PCR pipe successively, keep round end on the sub-lid of each PCR pipe protruding successively by the gap between two disks of scroll wheel 2, pressure on two disk borders makes each lid by PCR pipe rolled across be covered tightly.
Single job can compress the lid of a row (as 12) PCR pipe, then operates until compress the sub-lid of all PCR pipe equally, guarantees that in PCR reaction, reactant liquor can not be escaped because of evaporation.Because have employed the scroll wheel 2 of double-arc spline and the design of handle handle 1, not only efficiency is higher than pure manual manipulation, and apply moment, make to act on the large of the pure hand of force rate on pipe lid, lid is easily made to cover tightly, the scroll wheel 2 of double-deck disk designs and also makes capable point of action more accurate, can directly be acted in the plane of the surrounding of round end projection on the sub-lid of PCR pipe by disk outer rim.For PCR pipe of proper alignment on PCR instrument device, because the spacing is in length and breadth all equal, also can take laterally to compress once, longitudinally compress and once come dually to guarantee compression effect.
For the sub-lid of single PCR pipe that is fragmentary or scroll wheel malcompression, the extrusion ring 3 of handle 1 other end can be adopted.During operation, first horizontal positioned PCR pipe to be operated is on PCR flat board or in PCR instrument, unclamp extrusion ring locking spiral pin 4, then forward and inverse hour hands rotary squeezing ring 3, regulate the distance between each circular arc block of extrusion ring 3, the round end that the space made it in the annulus of its composition just in time holds on the sub-lid of PCR pipe of needs operation is protruding, then tightens extrusion ring locking spiral pin 4, the diameter of the distance between each circular arc block of fixed press ring 3 and the annulus of its composition.Operator holds handle 1 equally, and the one end maintaining extrusion ring 3 down, handle 1 vertical direction is horizontal by 90 degree of right angles, the round end aiming at PCR pipe lid to be operated with the inner space that extrusion ring 3 is formed is protruding, and make extrusion ring 3 vertically push down the planar section of the protruding surrounding of round end of PCR pipe lid, hold handle 1 firmly to press down, the operation of the compression lid to this PCR pipe can be completed.This operates more laborsaving than the round end bossing pressing with finger merely PCR pipe lid and effective (cannot realize pressing the peripheral portion of the round end projection of PCR pipe lid with finger, because this area is too little, finger cannot fully contact).
beneficial effect
The present invention is used for carrying out compression operation to PCR pipe in batch or single PCR pipe, the lid of PCR pipe is made to cover compression completely, prevent the liquid in the process of PCR reaction in PCR pipe from escaping because of evaporation, ensure the integraty of PCR reaction system, PCR is reacted and carries out smoothly.The present invention can be widely used in teaching, the scientific research activity of biology laboratory, especially needs the laboratory of carrying out PCR reaction in enormous quantities.The present invention is simple to operate, efficient, and can guarantee that its lid all covers tightly at short notice to large batch of PCR pipe after outfit, substantially increase work efficiency, simultaneously laborsaving than pure manual manipulation, accuracy rate is high.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Number in the figure: 1-handle; 2-scroll wheel; 3-extrusion ring; 4-extrusion ring locking spiral pin; 5-arc concave shape groove.
Detailed description of the invention
Below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in for illustration of the present invention to limit the scope of the invention.In addition should be understood that those skilled in the art can make various changes or modifications the present invention, and these equivalent form of values fall within the application's appended claims limited range equally after the content of having read the present invention's instruction.
As shown in Figure 1, use for laboratory round end PCR pipe lid pinch device, comprise handle 1, scroll wheel 2, extrusion ring 3 and extrusion ring locking spiral pin 4, handle 1 one end has U-shaped groove, be provided with scroll wheel 2 in groove, handle 1 centre portion is distributed with some arc concave shape grooves 5, and handle 1 other end has extrusion ring 3, extrusion ring 3 is separated circular arc block composition, and each circular arc block that extrusion ring locking spiral pin 4 can lock extrusion ring 3 makes it relatively to fix.
Described scroll wheel 2 is double-deck disk connection structure, distance between the center of circle of described two disks is less than the diameter of the sub-lid of PCR pipe of 0.2ml, circular protrusions tabling on the sub-lid of PCR pipe of the void shape between two disks and 0.2ml, also namely in this structure by the arc curve tabling of the projection on the indent arc curve of section at the center of two disks and the sub-lid of PCR pipe of 0.2ml.
Described extrusion ring 3 is 2-4 sheet circular arc block, and it can form complete annulus.
The circular arc block of described extrusion ring 3 can integral-rotation, and the space after rotating between circular arc block becomes large or diminishes, thus the space that circular arc block is comprised becomes large or diminishes.
The diameter of the circular arc of described arc concave shape rib 5 on handle 1 vertical direction conforms to the finger diameter of adult or bigger.
Described scroll wheel 2 is made up of timber, rigid plastic or metal etc.
Described handle 1, extrusion ring 3 and extrusion ring locking spiral pin 4 is made up of plastics or metal etc.
Described handle 1 is oval column type.
When school duty room have collected the great many of experiments PCR pipe of student, or R&D units completes the PCR response preparation of great amount of samples, for PCR pipe lid with round end projection, to build PCR pipe (or 12 PCR pipe of lid, 96 PCR pipe plates etc.) fitly lie in a horizontal plane on PCR pipe flat board or in PCR instrument, operator grasps handle 1 of the present invention, for ensureing to grip, can finger be embedded in the inner groovy 5 on handle, another hand steered firmly PCR pipe flat board or PCR instrument, handle 1 is made to have one end of U-shaped groove down, the round end aimed on PCR pipe lid in a row with the gap between two disks of scroll wheel 2 is protruding, make the gap between protruding two disks imbedding scroll wheel 2 of the round end on first initial PCR pipe lid, then make handle 1 vertical direction after 30 degree-an angle of 90 degrees, firmly press down handle 1, keep a lower pressure degree, and along the slow mobile handle 1 of orientation of PCR pipe, scroll wheel 2 is made to roll across above the lid of this row's PCR pipe successively, and keep round end on the sub-lid of each PCR pipe protruding successively by the gap between two disks of scroll wheel 2, the direction of such scroll wheel 2 can not be departed from, pressure simultaneously on two disk borders makes each lid by PCR pipe rolled across be covered tightly.Single job can compress the lid of a row (as 12) PCR pipe, then operates until compress the sub-lid of all PCR pipe equally, guarantees that in PCR reaction, reactant liquor can not be escaped because of evaporation.Because have employed the scroll wheel 2 of double-arc spline and the design of handle handle 1, not only efficiency is higher than pure manual manipulation, and apply moment, make to act on the large of the pure hand of force rate on pipe lid, lid is easily made to cover tightly, the scroll wheel 2 of double-deck disk designs and also makes capable point of action more accurate, can directly be acted in the plane of the surrounding of round end projection on the sub-lid of PCR pipe by disk outer rim.For PCR pipe of proper alignment on PCR instrument device, because the spacing is in length and breadth all equal, also can take laterally to compress once, longitudinally compress and once come dually to guarantee compression effect.
For the sub-lid of single PCR pipe that is fragmentary or scroll wheel malcompression, the extrusion ring 3 of handle 1 other end can be adopted.During operation, first horizontal positioned PCR pipe to be operated is on PCR flat board or in PCR instrument, unclamp extrusion ring locking spiral pin 4, then forward and inverse hour hands rotary squeezing ring 3, regulate the distance between each circular arc block of extrusion ring 3, the round end that the space made it in the annulus of its composition just in time holds on the sub-lid of PCR pipe of needs operation is protruding, then tightens extrusion ring locking spiral pin 4, the diameter of the distance between each circular arc block of fixed press ring 3 and the annulus of its composition.Operator holds handle 1 equally, and the one end maintaining extrusion ring 3 down, handle 1 vertical direction is horizontal by 90 degree of right angles, the round end aiming at PCR pipe lid to be operated with the inner space that extrusion ring 3 is formed is protruding, and make extrusion ring 3 vertically push down the planar section of the protruding surrounding of round end of PCR pipe lid, hold handle 1 firmly to press down, the operation of the compression lid to this PCR pipe can be completed.This operates more laborsaving than the round end bossing pressing with finger merely PCR pipe lid and effective (cannot realize pressing the peripheral portion of the round end projection of PCR pipe lid with finger, because this area is too little, finger cannot fully contact).
Need for the similar of other non-PCR reactions the operation covering tightly pipe lid, as long as the structure of pipe and lid is applicable to, such as enzyme is lived and is reacted, and test chemical etc., all can apply the present invention.
Claims (5)
1. a use for laboratory round end PCR pipe lid pinch device, is characterized in that locking spiral pin by handle, scroll wheel, extrusion ring and extrusion ring forms; Wherein, described handle one end has U-shaped groove, and the two-arm of U-shaped groove is fixed at the two ends of the axle of described scroll wheel, makes scroll wheel flexible rotating in U-shaped groove; Described extrusion ring is arranged at the handle other end, and described extrusion ring is made up of spaced some circular arc blocks, and described extrusion ring locking spiral pin, for locking each circular arc block of extrusion ring, makes it relatively to fix; Described handle centre portion is cylindrical body, and cylindrical body is provided with some arc concave shape grooves at interval in the upper and lower, so that finger grip; Described scroll wheel is double-deck disk connection structure, the circular protrusions tabling on the sub-lid of PCR pipe of the void shape between two disks and 0.2ml.
2. use for laboratory round end PCR pipe lid pinch device according to claim 1, it is characterized in that, described extrusion ring is made up of 2-4 sheet circular arc block, and these circular arc blocks surround a complete annulus.
3. use for laboratory round end PCR pipe lid pinch device according to claim 1, is characterized in that, the diameter of the circular arc of described arc concave shape groove on handle vertical direction conforms to the finger diameter of adult or bigger.
4. use for laboratory round end PCR pipe lid pinch device according to claim 1, is characterized in that, described handle entirety is column type.
5. the using method of use for laboratory round end PCR pipe lid pinch device as described in one of claim 1-4, is characterized in that,
(1) for PCR pipe lid with round end projection, the PCR pipe building lid is fitly lain in a horizontal plane on PCR pipe flat board or in PCR instrument, operator grasps handle, another hand steered firmly PCR pipe flat board or PCR instrument, handle is made to have one end of U-shaped groove down, the round end that gap between two of scroll wheel disks is aimed on PCR pipe lid in a row is protruding, make the gap between protruding two disks imbedding scroll wheel of the round end on first initial PCR pipe lid, then make handle vertical direction after 30 degree-an angle of 90 degrees, firmly press down handle, and along the slow mobile handle of orientation of PCR pipe, scroll wheel is made to roll across above the lid of this row's PCR pipe successively, keep round end on the sub-lid of each PCR pipe protruding successively by the gap between two disks of scroll wheel, pressure on two disk borders makes each lid by PCR pipe rolled across be covered tightly,
(2) for the sub-lid of single PCR pipe that is fragmentary or scroll wheel malcompression, the extrusion ring of the handle other end is adopted; During operation, first horizontal positioned PCR pipe to be operated is on PCR flat board or in PCR instrument, unclamp extrusion ring locking spiral pin, then forward and inverse hour hands rotary squeezing ring, regulate the distance between each circular arc block of extrusion ring, the round end that the space made it in the annulus of its composition just in time holds on the sub-lid of PCR pipe of needs operation is protruding, then tightens extrusion ring locking spiral pin, the diameter of the distance between each circular arc block of fixed press ring and the annulus of its composition; Operator holds handle, and the one end maintaining extrusion ring down, handle vertical direction is horizontal by 90 degree of right angles, the round end aiming at PCR pipe lid to be operated with the inner space that extrusion ring is formed is protruding, and make extrusion ring vertically push down the planar section of the protruding surrounding of round end of PCR pipe lid, hold handle firmly to press down, namely complete the operation of the compression lid to this PCR pipe.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310645637.1A CN103641049B (en) | 2013-12-05 | 2013-12-05 | A kind of use for laboratory round end PCR pipe lid pinch device and using method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310645637.1A CN103641049B (en) | 2013-12-05 | 2013-12-05 | A kind of use for laboratory round end PCR pipe lid pinch device and using method thereof |
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| CN103641049A CN103641049A (en) | 2014-03-19 |
| CN103641049B true CN103641049B (en) | 2015-07-29 |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1082753A1 (en) * | 1982-08-09 | 1984-03-30 | Предприятие П/Я Р-6118 | Device for closing glass containers with metal lids |
| CN86200295U (en) * | 1986-02-04 | 1986-09-17 | 薛光威 | Pocket-size sealing machine for glass can |
| JPH08244888A (en) * | 1995-03-09 | 1996-09-24 | Shibuya Kogyo Co Ltd | Method and device for clamping of cap |
| CN2649565Y (en) * | 2003-07-15 | 2004-10-20 | 徐开定 | Cover-rolling machine |
| FR2865457A1 (en) * | 2004-01-23 | 2005-07-29 | Greiner Bio One Gmbh | Assembling a cap on a receiving vessel, useful for holding body fluids or tissue samples, by applying, between cap and vessel, a force that causes rotational or revolving movement |
| CN202988673U (en) * | 2012-08-31 | 2013-06-12 | 宁波松鹤文具有限公司 | Cover lading machine used for outer shell of solid gum product |
| CN203639118U (en) * | 2013-12-05 | 2014-06-11 | 复旦大学 | Round-ended polymerase chain reaction (PCR) tube cap compressing apparatus for laboratories |
-
2013
- 2013-12-05 CN CN201310645637.1A patent/CN103641049B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1082753A1 (en) * | 1982-08-09 | 1984-03-30 | Предприятие П/Я Р-6118 | Device for closing glass containers with metal lids |
| CN86200295U (en) * | 1986-02-04 | 1986-09-17 | 薛光威 | Pocket-size sealing machine for glass can |
| JPH08244888A (en) * | 1995-03-09 | 1996-09-24 | Shibuya Kogyo Co Ltd | Method and device for clamping of cap |
| CN2649565Y (en) * | 2003-07-15 | 2004-10-20 | 徐开定 | Cover-rolling machine |
| FR2865457A1 (en) * | 2004-01-23 | 2005-07-29 | Greiner Bio One Gmbh | Assembling a cap on a receiving vessel, useful for holding body fluids or tissue samples, by applying, between cap and vessel, a force that causes rotational or revolving movement |
| CN202988673U (en) * | 2012-08-31 | 2013-06-12 | 宁波松鹤文具有限公司 | Cover lading machine used for outer shell of solid gum product |
| CN203639118U (en) * | 2013-12-05 | 2014-06-11 | 复旦大学 | Round-ended polymerase chain reaction (PCR) tube cap compressing apparatus for laboratories |
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| CN103641049A (en) | 2014-03-19 |
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Granted publication date: 20150729 Termination date: 20171205 |