CN218561444U

CN218561444U - Anti-pollution PCR reaction tube

Info

Publication number: CN218561444U
Application number: CN202222306120.7U
Authority: CN
Inventors: 骆攀; 王东风
Original assignee: Zhengzhou Meiling Biotechnology Co ltd
Current assignee: Zhengzhou Meiling Biotechnology Co ltd
Priority date: 2022-08-30
Filing date: 2022-08-30
Publication date: 2023-03-03
Anticipated expiration: 2032-08-30

Abstract

The utility model discloses an anti-pollution PCR reaction tube, its technical scheme main points are: including the PCR reaction tube, the top surface of PCR reaction tube is provided with the board that turns over, anti-pollution subassembly sets up the bottom surface of board that turns over for the inside of preventing the PCR reaction tube receives the pollution, anti-pollution subassembly includes: the connecting pipe, connecting pipe fixed mounting is in the bottom surface of turning over the board, the excircle wall of turning over the board is fixed to have cup jointed first sealing ring, the bottom surface fixed mounting of PCR reaction pipe has the second sealing ring, the interior round wall of PCR reaction pipe with the connecting pipe activity cup joints, first sealing ring with the activity of second sealing ring is cup jointed, uses through mutually supporting of PCR reaction pipe, elastic ring, gripping plate, limiting plate, joint groove, seal groove, third sealing ring, arc wall and recess, can reach the effect that prevents the pollutant from getting into the inside of PCR reaction pipe, prevents to cause the influence to the experimental result.

Description

Anti-pollution PCR reaction tube

Technical Field

The utility model relates to a PCR reaction tube technical field, concretely relates to anti-pollution PCR reaction tube.

Background

The PCR is a technology for amplifying nucleic acid in vitro developed in the last 80 th century, has the outstanding advantages of specificity, sensitivity, high yield, rapidness, simplicity, good repeatability, easy automation and the like, can amplify a target gene or a certain DNA fragment to be researched to hundreds of thousands to millions of times within a test tube, enables direct observation and judgment by naked eyes, can amplify sufficient DNA from a hair, a drop of blood and even a cell for analysis, research and detection and identification, is a revolutionary innovation and a milestone in the field of biotechnology, and particularly shows an irreplaceable side in the field of food safety detection.

Among the prior art, PCR reaction pipe is in the use, because the tube cap adopts the mode of hugging closely the contact with the mouth of pipe closed, often has the relatively poor problem of fastness and leakproofness between tube cap and the mouth of pipe, heats repeatedly when the operation and makes inside air expend with heat and contract with cold follow gap business turn over between tube cap and the mouth of pipe, and then leads to outside air to get into the formation aerosol pollution of PCR reaction pipe, often can cause the influence to the experimental result of PCR reaction pipe.

SUMMERY OF THE UTILITY MODEL

The utility model provides a not enough to prior art, the utility model provides an anti-pollution PCR reaction tube has solved PCR reaction tube in the use, because the tube cap adopts the mode closure of hugging closely the contact with the mouth of pipe, often there is the relatively poor problem of fastness and leakproofness between tube cap and the mouth of pipe, heat repeatedly when the operation makes inside air expend with heat and contract with cold follow gap business turn over between tube cap and the mouth of pipe, and then lead to outside air to get into PCR reaction tube and form aerosol pollution, often can cause the problem of influence to PCR reaction tube's experimental result.

The above technical object of the present invention can be achieved by the following technical solutions:

a contamination-resistant PCR reaction tube, comprising: PCR reaction tube, PCR reaction tube's top surface is provided with the board that turns over, anti-pollution subassembly sets up the bottom surface of turning over the board for prevent that PCR reaction tube's inside from receiving the pollution, anti-pollution subassembly includes: the connecting pipe, the connecting pipe fixed mounting be in the bottom surface of stirring board, the excircle wall of stirring board is fixed to have cup jointed first sealing ring, the bottom surface fixed mounting of PCR reaction pipe has the second sealing ring, the interior circle wall of PCR reaction pipe with the connecting pipe activity cup joints, first sealing ring with the activity of second sealing ring is cup jointed, the excircle wall fixed mounting of stirring board has the gripping plate, the excircle wall fixed mounting of stirring board has the connecting strip, the connecting strip with the excircle wall fixed mounting of PCR reaction pipe, the bottom surface fixed mounting of gripping plate has the connecting plate, one side fixed mounting of connecting plate has the limiting plate, the bottom surface of gripping plate is provided with the joint soon, the arc wall has been seted up to the fast one side of joint, the joint soon with PCR reaction pipe fixed mounting, a plurality of joint groove has been seted up to the fast one side of joint, the joint groove with the limiting plate activity cup joints.

In order to seal up the gap between connecting pipe and the PCR reaction pipe, conduct the utility model discloses an anti-pollution PCR reaction pipe, the preferred, the seal groove has been seted up to the interior circle wall of PCR reaction pipe, the third sealing ring has been cup jointed in the activity of the interior circle wall of seal groove, the third sealing ring with the outer circle wall of connecting pipe is fixed to be cup jointed.

In order to improve the friction between the fingers and the gripping plate, the utility model discloses an anti-pollution PCR reaction tube, the preferred, a plurality of recess has been seted up respectively to the top surface and the bottom surface of gripping plate.

In order to place PCR reaction tube, conduct the utility model discloses an anti-pollution PCR reaction tube, the preferred, the bottom surface of turning over the board is provided with places the piece, the supporting hole has been seted up to the top surface of placing the piece, the supporting hole with the activity of PCR reaction tube is cup jointed.

In order to carry out the fixed of certain degree to the PCR reaction tube, as the utility model discloses an anti-pollution PCR reaction tube, the preferred, the fixed elastic ring that cup joints of interior round wall in support hole.

In order to support PCR reaction tube bottom, as the utility model discloses an anti-pollution PCR reaction tube, the preferred, place one side of piece and seted up the side opening, the inside bottom surface fixed mounting of side opening has the support column.

In order to protect the bottom surface of the PCR reaction tube, the utility model discloses an anti-pollution PCR reaction tube, the preferred, the top surface fixed mounting of support column has the rubber pad.

To sum up, the utility model discloses mainly have following beneficial effect:

through mutually supporting use of PCR reaction tube, elastic ring, gripping board, limiting plate, joint groove, seal groove, third sealing ring, arc wall and recess, can reach the effect that prevents the pollutant from getting into the inside of PCR reaction tube, prevent to cause the influence to the experimental result.

Through the seal groove, the third sealing ring, mutually supporting of PCR reaction pipe and connecting pipe is used, can reach the effect of sealing up the gap between connecting pipe and the PCR reaction pipe, use through mutually supporting of recess and grip plate, can reach the effect that improves frictional force between finger and the grip plate, use through mutually supporting of brace hole and PCR reaction pipe, can reach the effect of placing PCR reaction pipe, use through mutually supporting of elastic ring and brace hole, can reach the effect of carrying out the fixed of certain degree to PCR reaction pipe, use through mutually supporting of support column and placing piece, can reach the effect of carrying out the support to PCR reaction socle portion.

Drawings

FIG. 1 is a perspective view of the present invention;

FIG. 2 is a schematic diagram of the PCR reaction tube structure of the present invention;

fig. 3 is a schematic diagram of the structure of the turning plate of the present invention;

fig. 4 is a schematic view of the snap fastener structure of the present invention.

Reference numerals: 1. a PCR reaction tube; 2. turning over the board; 3. a connecting pipe; 4. a first seal ring; 5. a second seal ring; 6. a gripping plate; 7. a connecting plate; 8. a limiting plate; 9. the clamping is quick; 10. a clamping groove; 11. a sealing groove; 12. a third seal ring; 13. an arc-shaped slot; 14. a groove; 15. a connecting strip; 16. placing the blocks; 17. a support hole; 18. an elastic ring; 19. a side hole; 20. a support pillar; 21. a rubber cushion.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1, 2, 3 and 4, a contamination-proof PCR reaction tube includes: PCR reaction tube 1, PCR reaction tube 1's top surface is provided with turns over board 2, and the bottom surface that turns over board 2 is provided with anti-pollution subassembly for prevent that PCR reaction tube 1's inside from receiving the pollution, anti-pollution subassembly includes: the connecting pipe 3, the connecting pipe 3 is fixedly arranged on the bottom surface of the turning plate 2, the excircle wall surface of the turning plate 2 is fixedly sleeved with a first sealing ring 4, the top surface diameter of the first sealing ring 4 is larger, the bottom surface diameter is smaller, the bottom surface of the PCR reaction pipe 1 is fixedly provided with a second sealing ring 5, the top surface diameter of the second sealing ring 5 is larger, the bottom surface diameter is smaller, so that the outer part of the first sealing ring 4 and the inner part of the second sealing ring 5 can be tightly attached together, the inner circle wall surface of the PCR reaction pipe 1 is movably sleeved with the connecting pipe 3, the first sealing ring 4 is movably sleeved with the second sealing ring 5, the excircle wall surface of the turning plate 2 is fixedly provided with a gripping plate 6, the gripping plate 6 has better toughness, the excircle wall surface of the turning plate 2 is fixedly provided with a connecting strip 15, the connecting strip 15 is fixedly arranged with the excircle wall surface of the PCR reaction pipe 1, the bottom surface of the gripping plate 6 is fixedly provided with a connecting plate 7, a limiting plate 8 is fixedly arranged on one side of a connecting plate 7, a clamping block 9 is arranged on the bottom surface of a grasping plate 6, an arc-shaped groove 13 is formed in one side of the clamping block 9, the clamping block 9 and a PCR reaction tube 1 are fixedly arranged, a plurality of clamping grooves 10 are formed in one side of the clamping block 9, the clamping grooves 10 are movably sleeved with the limiting plate 8, through the arranged PCR reaction tube 1, when the PCR reaction tube 1 needs to be used, the PCR reaction tube 1 is taken out from the inner part of an elastic ring 18, then the PCR reaction tube 1 is grasped by one hand, the grasping plate 6 is grasped by fingers of one hand and pulled upwards, the grasping plate 6 has good toughness, the limiting plate 8 is arranged in the clamping grooves 10, the connecting plate 7, the limiting plate 8, the clamping block 9 and the clamping grooves 10 form a clamping structure, the first sealing ring 4 is pulled to drive the connecting plate 7 and the limiting plate 8 to move, the limiting plate 8 to move leftwards and then is not clamped with the clamping grooves 10, thereby relieving the restriction to the turning plate 2, after the turning plate 2 is rotated and opened, adding the solution into the interior of the PCR reaction tube 1, poking the turning plate 2 by hand, enabling the connecting pipe 3 to enter the inner circular wall surface of the PCR reaction tube 1, pressing downwards to enable the third sealing ring 12 to enter the interior of the sealing groove 11, because the sealing groove 11 has better elasticity, thereby extruding the inner walls of the PCR reaction tube 1 and the PCR reaction tube 1 of the rubber gasket 21, thereby sealing the gap therebetween, pressing the turning plate 2 while enabling the interiors of the first sealing ring 4 and the second sealing ring 5 to be extruded, and the limiting plate 8 and the clamping block 9 are clamped to fix the turning plate 2 on the top surface of the PCR reaction tube 1, because the first sealing ring 4 and the second sealing ring 5 have better elasticity, the limiting plate 8 can be further pressed and moved to enter the clamping groove 10 at the bottom, so that the turning plate 2 and the PCR reaction tube 1 are fixed more tightly, the sealing effect between the second sealing ring 5 and the first sealing ring 4 is better, and because the clamping block 8 and the clamping block 9 are clamped to enable the interior of the limiting plate to be clamped so that the air is difficult to be pushed open, thereby preventing the PCR reaction tube 1 from being contaminated, and preventing the PCR reaction tube 1 from being unable to be discharged.

Referring to fig. 2, 3 and 4, the inner circular wall of the PCR reaction tube 1 is provided with a sealing groove 11, the inner circular wall of the sealing groove 11 is movably sleeved with a third sealing ring 12, the third sealing ring 12 is fixedly sleeved with the outer circular wall of the connecting tube 3, the top surface and the bottom surface of the grip plate 6 are respectively provided with a plurality of grooves 14, the bottom surface of the tilting plate 2 is provided with a placing block 16, the top surface of the placing block 16 is provided with a supporting hole 17, the supporting hole 17 is movably sleeved with the PCR reaction tube 1, the inner circular wall of the supporting hole 17 is fixedly sleeved with an elastic ring 18, the elasticity of the elastic ring 18 is better, the inner diameter is smaller than the outer diameter of the PCR reaction tube 1, one side of the placing block 16 is provided with a side hole 19, the inner bottom surface of the side hole 19 is fixedly provided with a supporting column 20, the top surface of the supporting column 20 is fixedly provided with a rubber pad 21, the sealing groove 11 and the third sealing ring 12 are passed through, seal groove 11 and third sealing ring 12 can seal up the gap between connecting pipe 3 and the PCR reaction pipe 1, through the recess 14 that sets up, recess 14 can improve the frictional force between finger and grip plate 6, support hole 17 through setting up, place PCR reaction pipe 1 in the inside of support hole 17, can support PCR reaction pipe 1 and place, elastic ring 18 through the setting, elastic ring 18 gets inside diameter ratio PCR reaction pipe 1's outside diameter and is little, but elastic ring 18's elasticity is better, when inserting PCR reaction pipe 1 in elastic ring 18's inside, elastic ring 18 extrudees PCR reaction pipe 1's outside and then increases the frictional force with PCR reaction pipe 1, it is fixed to carry out certain degree to PCR reaction pipe 1, support column 20 through setting up, joint groove 10 can support PCR reaction pipe 1's bottom.

The working principle is as follows: referring to fig. 1-4, with the PCR reaction tube 1, when the PCR reaction tube 1 is needed, the PCR reaction tube 1 is taken out from the interior of the elastic ring 18, then the PCR reaction tube 1 is grasped by one hand, the grasping plate 6 is grasped by fingers of one hand and pulled upwards, the grasping plate 6 has good toughness, the limiting plate 8 is located inside the clamping groove 10, so that the connecting plate 7, the limiting plate 8, the clamping block 9 and the clamping groove 10 form a clamping structure, pulling the first sealing ring 4 can drive the connecting plate 7 and the limiting plate 8 to move, so that the limiting plate 8 moves leftwards and is not clamped with the clamping groove 10, so as to release the limitation on the flip plate 2, after the flip plate 2 is rotated and opened, after a solution is added into the interior of the PCR reaction tube 1, the flip plate 2 is pulled by hand, so that the connecting tube 3 enters the inner circular wall surface of the PCR reaction tube 1, the third sealing ring 12 enters the interior of the sealing groove 11 by pressing downwards, because the sealing groove 11 has good elasticity, thereby pressing the inner walls of the PCR reaction tube 1 and the sealing ring 4 of the second sealing ring 2, and the second sealing ring 5 can be pressed tightly, and the second sealing ring 2 can be pressed tightly, and the clamping groove 2, and the second sealing ring 2 can be pressed tightly, and the sealing ring 5 tightly, and the second sealing ring 2, thereby preventing the air inside the PCR reaction tube 1 from being discharged and the external air from entering, and preventing the pollution to the inside of the PCR reaction tube 1.

Through seal groove 11, third sealing ring 12, seal groove 11 and third sealing ring 12 can seal up the gap between connecting pipe 3 and the PCR reaction pipe 1, through the recess 14 that sets up, recess 14 can improve the frictional force between finger and grip plate 6, through the supported hole 17 that sets up, place PCR reaction pipe 1 in the inside of supported hole 17, can support PCR reaction pipe 1 and place, elastic ring 18 through the setting, the inside diameter ratio of elastic ring 18 is little than the outside diameter of PCR reaction pipe 1, but the elasticity of elastic ring 18 is better, when inserting PCR reaction pipe 1 in the inside of elastic ring 18, elastic ring 18 extrudees the outside of PCR reaction pipe 1 and then increases the frictional force with PCR reaction pipe 1, carry out the fixing of certain degree to PCR reaction pipe 1, through the support column 20 that sets up, joint groove 10 can support the bottom of PCR reaction pipe 1.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. An anti-contamination PCR reaction tube, comprising:

the device comprises a PCR reaction tube (1), wherein a turning plate (2) is arranged on the top surface of the PCR reaction tube (1);

an anti-contamination assembly provided on a bottom surface of the flip plate (2) for preventing the inside of the PCR reaction tube (1) from being contaminated, the anti-contamination assembly comprising: connecting pipe (3), connecting pipe (3) fixed mounting be in the bottom surface of turning over movable plate (2), the excircle wall of turning over movable plate (2) is fixed to have cup jointed first sealing ring (4), the bottom surface fixed mounting of PCR reaction pipe (1) has second sealing ring (5), the interior circle wall of PCR reaction pipe (1) with connecting pipe (3) activity is cup jointed, first sealing ring (4) with second sealing ring (5) activity is cup jointed, the excircle wall fixed mounting of turning over movable plate (2) has grasping plate (6), the excircle wall fixed mounting of turning over movable plate (2) has connecting strip (15), connecting strip (15) with the excircle wall fixed mounting of PCR reaction pipe (1), grasping plate's (6) bottom surface fixed mounting has connecting plate (7), one side fixed mounting of connecting plate (7) has limiting plate (8), grasping plate's (6) bottom surface is provided with joint (9), arc wall (13) have been seted up to one side of joint (9), joint (9) with PCR reaction pipe (1) fixed mounting has seted up a plurality of joint groove (10).

2. The anti-contamination PCR reaction tube according to claim 1, wherein: the inner circle wall surface of the PCR reaction tube (1) is provided with a sealing groove (11), the inner circle wall surface of the sealing groove (11) is movably sleeved with a third sealing ring (12), and the third sealing ring (12) is fixedly sleeved with the outer circle wall surface of the connecting tube (3).

3. The anti-contamination PCR reaction tube according to claim 1, wherein: the top surface and the bottom surface of the gripping plate (6) are respectively provided with a plurality of grooves (14).

4. The anti-contamination PCR reaction tube according to claim 1, wherein: the bottom surface of the turning plate (2) is provided with a placing block (16), the top surface of the placing block (16) is provided with a supporting hole (17), and the supporting hole (17) is movably sleeved with the PCR reaction tube (1).

5. The anti-contamination PCR reaction tube according to claim 4, wherein: an elastic ring (18) is fixedly sleeved on the inner circle wall surface of the supporting hole (17).

6. The anti-contamination PCR reaction tube according to claim 5, wherein: a side hole (19) is formed in one side of the placing block (16), and a supporting column (20) is fixedly mounted on the bottom surface of the inside of the side hole (19).

7. The contamination-resistant PCR reaction tube of claim 6, wherein: and a rubber pad (21) is fixedly mounted on the top surface of the supporting column (20).