CN203534964U - Surface plasma resonance biosensing device based on metal raster - Google Patents
Surface plasma resonance biosensing device based on metal raster Download PDFInfo
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- CN203534964U CN203534964U CN201320654940.3U CN201320654940U CN203534964U CN 203534964 U CN203534964 U CN 203534964U CN 201320654940 U CN201320654940 U CN 201320654940U CN 203534964 U CN203534964 U CN 203534964U
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Abstract
The utility model discloses a surface plasma resonance biosensing device based on a metal raster, and belongs to the technical field of sensing. The device consists of a laser light source, a polarizer, a focusing lens, a fiber optic spectrometer and a computer, wherein a raster surface plasma sensor is arranged between the focusing lens and the fiber optic spectrometer; the raster surface plasma sensor consists of a platform, a raster body, a metallic membrane and a sample cell. In detection, a sample to be detected is placed on the sample cell of the raster surface plasma sensor; lights sent by the laser light source in the horizontal direction is converted into P polarized lights through the focusing lens and the polarizer; the P polarized lights enter into the fiber optic spectrometer through raster reflected lights of the metallic membrane to obtain detected surface plasma signals to be sent to the computer to be processed. Through the comparison of difference of the signals without sample or having sample, the refractive index of the detected solution and information of related concentration can be obtained. The device is simple in technology, convenient to operate and control and high in accuracy.
Description
Technical field
The utility model belongs to field of sensing technologies, is a kind of sensor detecting for material.
Background technology
Surface plasmon resonance (Surface Plasmon Resonance, SPR) by the electric density yo-yo effect on metal and dielectric interface that is present in that electromagnetic wave excited, very responsive to surrounding medium variations in refractive index, it is a kind of optical sensing technology, it is without carrying out sample mark, and the interaction between energy Real-Time Monitoring biomolecule, becomes the tool of biomolecule monitoring in recent years.And at present, the sensor construction based on SPR effect is also widely used in the fields such as biochemical and environment monitoring, nano-photon device, and makes substantial progress in fields such as genetic test, industrial humidity controls.
When incident light is in visible ray and near-infrared band, the real part of most dielectric constant of metal is negative, so metal is adjacent the specific inductive capacity contrary sign of medium, only has P type polarized light just can inspire surface plasma excimer (SPP).When P type polarized light total reflection will occur when being greater than the angle incident medium of the cirtical angle of total reflection, a part of incident light infiltrates through metal film by the form with evanescent wave.When incident angle meets certain condition, evanescent wave is parallel to the wave vector at metal/dielectric interface and the wave vector of SPP equates, there is strong coupling in these two kinds of patterns, the energy of incident light is transferred to some in SPP, cause intensity of reflected light significantly to reduce, there is minimum value in reflectivity, and then occurs SPR effect.
Generally the SPR device of application is Kretchman (Kretschmann) prism-type device now, and it is comprised of prism, metallic film, surrounding medium.The plasma resonance biosensing device that adopts Kretchman (Kretschmann) prism-type device to make for basis consists of LASER Light Source, the polarizer, condenser lens, prism-type device, fiber spectrometer and computing machine, the light of the horizontal direction that LASER Light Source is sent, through the polarizer and condenser lens, obtains P polarized light; This P polarized light enters through prism-type device the signal that fiber spectrometer obtains detecting, and delivers to computing machine and processes, thereby obtain the refractive index of measured object solution and the information of related concentrations.
Light excites the SPR effect of thin metal layer through prism, and testing medium is positioned at the opposite side of metal film, the impact of therefore having avoided the variation of determinand surrounding environment to bring to measurement.But only have the metal film of thickness in a certain particular range just can excite comparatively desirable SPR effect, therefore there is certain difficulty of processing, higher to technological requirement.And Kretschmann prism-coupled model spr sensor has mobile part, volume is large, need to accurately control angle, is not easy to integrated.
Summary of the invention
The technical problems to be solved in the utility model is to provide a kind of surface plasma resonance biosensor based on metal grating, thereby has reduced difficulty of processing, and testing result is more accurate.
The scheme of the utility model technical solution problem is to adopt LASER Light Source, the polarizer, condenser lens, fiber spectrometer and computing machine form, between condenser lens and fiber spectrometer, there is grating surface plasma sensor, to substitute prism-type device, grating surface plasma sensor is by platform, grating body, metal film and sample cell form, platform, grating body, metal film and sample cell are sheet, grating body is installed on platform, grating body upper surface metal-coated membrane, on metal film, be fixed with sample cell, wherein the grating cycle of grating body is 600nm, grating height is 100nm, it is 0.5 that grating accounts for wide ratio, metal film can be golden film or silverskin, gold film is best, its thickness is 50nm.During detection, on the sample cell of grating surface plasma sensor, place detected sample, the light of the horizontal direction that LASER Light Source is sent, through condenser lens and the polarizer, obtains P polarized light; This P polarized light through the optical grating reflection light of gold-plated film enter fiber spectrometer obtain detecting surface plasma signal, deliver to computing machine and process.By the signal difference of contrast n.s. and sample, thereby obtain the refractive index of measured object solution and the information of related concentrations.
Grating surface plasma sensor can be discoid, sample cell carries out fan-shaped two to six equal portions that are divided into from center, bearing is arranged at the bottom of platform, bearing is connected with axle, during detection, can in the sample cell that carries out equal portions, add testing sample, comprising sample cell equal portions that detect distilled water or other objects of reference, by axle and bearing, rotate grating surface plasma sensor, can complete and once automatically detect a plurality of testing samples.
The utility model has well made up the above defect of Kretschmann prism-coupled model spr sensor, it does not using prism as exciting media, the metal film that does not need specific thicknesses, can adopt the metal film of macroscopic thickness, and the optical quality of substrate do not have decisive influence to sensor performance yet.Compared with Kretschmann prism-coupled type spr sensor, manufacture craft is simple, control conveniently, and degree of accuracy is high.
Accompanying drawing explanation
Fig. 1 is the utility model schematic diagram;
Fig. 2 is grating surface plasma sensor schematic diagram;
Fig. 3 is sample cell schematic diagram.
Embodiment
The utility model is by LASER Light Source 1, the polarizer 2, condenser lens 3, fiber spectrometer 5 and 6 one-tenth of calculation mechanism, between condenser lens 3 and fiber spectrometer 5, there is grating surface plasma sensor 4, grating surface plasma sensor 4 is by platform 7, grating body 8, metal film 9 and sample cell 10 form, platform 7, grating body 8, metal film 9 and sample cell 10 are sheet, grating body 8 is installed on platform 7, grating body 8 upper surface metal-coated membranes 9, on metal film 9, be fixed with sample cell 10, wherein grating surface plasma sensor 4 is discoid, sample cell 10Cong carries out at center fan-shaped six equal portions that are divided into, bearing 12 is arranged at the bottom of platform 7, bearing 12 is connected with axle 11, the grating cycle of grating body 8 is 600nm, grating height is 100nm, it is 0.5 that grating accounts for wide ratio, metal film 9 is golden films, its thickness is 50nm.
Claims (3)
1. the surface plasma resonance biosensor based on metal grating, by LASER Light Source, the polarizer, condenser lens, fiber spectrometer and computing machine, formed, it is characterized in that: between condenser lens and fiber spectrometer, have grating surface plasma sensor, grating surface plasma sensor is by platform, grating body, metal film and sample cell form, platform, grating body, metal film and sample cell are sheet, grating body is installed on platform, grating body upper surface metal-coated membrane, is fixed with sample cell on metal film.
2. biosensing device according to claim 1, is characterized in that: the grating cycle of grating body is 600nm, and grating height is 100nm, and it is 0.5 that grating accounts for wide ratio, and metal film is golden film or silverskin, and thickness is 50nm.
3. biosensing device according to claim 1, is characterized in that: grating surface plasma sensor is discoid, and sample cell carries out fan-shaped two to six equal portions that are divided into from center, and bearing is arranged at the bottom of platform, and bearing is connected with axle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320654940.3U CN203534964U (en) | 2013-10-23 | 2013-10-23 | Surface plasma resonance biosensing device based on metal raster |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320654940.3U CN203534964U (en) | 2013-10-23 | 2013-10-23 | Surface plasma resonance biosensing device based on metal raster |
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| CN203534964U true CN203534964U (en) | 2014-04-09 |
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| CN201320654940.3U Expired - Fee Related CN203534964U (en) | 2013-10-23 | 2013-10-23 | Surface plasma resonance biosensing device based on metal raster |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107084752A (en) * | 2017-04-20 | 2017-08-22 | 电子科技大学 | Optical fiber microbubble concentration sensor and its method for sensing based on nano-gold film |
| CN109030412A (en) * | 2018-04-24 | 2018-12-18 | 江南大学 | A kind of measuring device for liquid refractive index and its method based on DVD optical grating construction |
| CN110261350A (en) * | 2019-06-04 | 2019-09-20 | 暨南大学 | A kind of optical sensor system and sensing testing method |
| CN113406017A (en) * | 2021-06-17 | 2021-09-17 | 桂林电子科技大学 | High-integration surface plasma resonance sensor system |
-
2013
- 2013-10-23 CN CN201320654940.3U patent/CN203534964U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107084752A (en) * | 2017-04-20 | 2017-08-22 | 电子科技大学 | Optical fiber microbubble concentration sensor and its method for sensing based on nano-gold film |
| CN109030412A (en) * | 2018-04-24 | 2018-12-18 | 江南大学 | A kind of measuring device for liquid refractive index and its method based on DVD optical grating construction |
| CN110261350A (en) * | 2019-06-04 | 2019-09-20 | 暨南大学 | A kind of optical sensor system and sensing testing method |
| CN113406017A (en) * | 2021-06-17 | 2021-09-17 | 桂林电子科技大学 | High-integration surface plasma resonance sensor system |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140409 Termination date: 20181023 |
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| CF01 | Termination of patent right due to non-payment of annual fee |