CN203520223U - Non-uniform high-gradient isolated-cell magnetic-field mechanics loading device - Google Patents
Non-uniform high-gradient isolated-cell magnetic-field mechanics loading device Download PDFInfo
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- CN203520223U CN203520223U CN201320620751.4U CN201320620751U CN203520223U CN 203520223 U CN203520223 U CN 203520223U CN 201320620751 U CN201320620751 U CN 201320620751U CN 203520223 U CN203520223 U CN 203520223U
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Abstract
The utility model discloses a non-uniform high-gradient isolated-cell magnetic-field mechanics loading device, and relates to biomedicine experimental apparatuses. The non-uniform high-gradient isolated-cell magnetic-field mechanics loading device comprises a magnetic-field mechanics loading assembly, a loading magnetic pole, a transverse magnetism conducting rod, a first magnetism conducting sleeve and a second magnetism conducting sleeve, wherein the U-shaped magnetic-field mechanics loading assembly is composed of a first magnetic pole fixing rod and a second magnetic pole fixing rod, the loading magnetic pole is composed of a flat-end magnetic pole and a magnetic pole kit with a replaceable tip, the flat-end magnetic pole is inserted into the first magnetic pole fixing rod, the magnetic pole kit with the replaceable tip comprises a set of cylindrical one-way tip magnetic poles with different gradients alpha, non-uniform magnetic fields which are different in gradient, intensity and direction can be obtained by rotating the angles of the one-way tip magnetic poles or replacing the one-way tip magnetic poles with the different gradients alpha, and the magnetic pole kit with the replaceable tip is inserted into the second magnetic pole fixing rod, and is arranged opposite to the flat-end magnetic pole, so that a non-uniform high-gradient magnetic-field mechanics loading area is formed. The non-uniform high-gradient isolated-cell magnetic-field mechanics loading device can carry out magnetic-field mechanics loading on various cells, is applicable to isolated cells with different capacities, and can apply shear stress with different gradients, different degrees of intensity and different directions to cytoskeletons of the isolated cells.
Description
Technical field
The utility model relates to biomedicine experiment device, relates in particular to a kind of biological scientific research apparatus of realizing isolated cells magnetic field Mechanical loading for generation of non-homogeneous high-gradient magnetic field.
Background technology
Cell research is the focus of current regenerative medicine research, and research in recent years shows physical factor in microenvironment---mechanical force on cell migration, differentiation and propagation have vital role.The rationality that stimulates checking cyto-mechanics network to exist by cell being loaded to mechanical stress, observes the deformation that loads cell skeleton, and the trend of differentiation.Chinese invention patent application " a kind of pulsed magnetic field apparatus for isolated cells " (Chinese invention patent application number: 201210492992.5 application publication numbers: CN102968154A) disclose a kind of pulsed magnetic field apparatus for the research of isolated cells biological effect of magnetic field, by frequency control circuit, control circuit of duty ratio, turn-on control circuit, electromagnetic switching device and field intensity control circuit, control power supply, sample circuit, sampling device, holding circuit, select circuit and A/D conversion to form with LED display circuit, electromagnetic switching device wherein has the magnetic core that a use " mouth " type silicon steel sheet heap is made, one side of magnetic core is wound with coil, another side has one as the small gap in magnetic fields region, at small gap gap location, be provided with gap adjustment block and auxiliary fixing plate, by changing the size of coil current and the distance in magnetic core gap, realizing the field intensity of pulsed magnetic field apparatus controls.
But, existing magnetic force charger only can change by changing the size of coil current and the distance in magnetic core gap the global magnetic field intensity in magnetic fields district, pretreated isolated cells is carried out to intensity and the uniform stress loading of direction, generation constant stress stimulates, the directional migration of induction isolated cells, the different parts cytoskeleton that is not also directed to individual cells carries out the magnetic force charger of stress loading.Existing magnetic force charger all can not load different mechanical stresses to cell different parts cytoskeleton to stimulate, and can not solve the technical matters of the shear stress that produces non-homogeneous high gradient.
Utility model content
The purpose of this utility model is to provide a kind of non-homogeneous high gradient isolated cells magnetic field mechanical loading unit, the magnetic field magnetic force direction producing and size can be adjusted with the needs of experiment, magnetic field intensity can be non-homogeneous high gradient and distribute in space, and the magnetic force that can meet different cell different directions and intensity loads.
The utility model solves the problems of the technologies described above adopted technical scheme:
A kind of non-homogeneous high gradient isolated cells magnetic field mechanical loading unit, comprise magnetic field mechanics charging assembly, load magnetic pole, for generation of controllable pulse power supply and the solenoid that is connected to controllable pulse power supply in magnetic field, and for detection of the gaussmeter of magnetic field intensity, it is characterized in that:
Described magnetic field mechanics charging assembly comprises magnetic conduction cross bar, the first magnetic conduction sleeve, the second magnetic conduction sleeve, the first magnetic pole fixed bar, the second magnetic pole fixed bar; The first described magnetic conduction sleeve is connected to one end of magnetic conduction cross bar by the first magnetic pole fixed bar, the second described magnetic conduction sleeve is connected to the other end of magnetic conduction cross bar by the second magnetic pole fixed bar; On the first magnetic conduction sleeve, the second magnetic conduction sleeve and/or magnetic conduction cross bar, the described solenoid of configuration, forms U-shaped magnetic field mechanics charging assembly;
Described loading magnetic pole is comprised of flush end magnetic pole and one group of replaceable most advanced and sophisticated magnetic pole external member; Described flush end magnetic pole inserts in the connecting hole of the first magnetic pole fixed bar;
Described replaceable most advanced and sophisticated magnetic pole external member comprises one group of cylindrical unidirectional tip magnetic pole with different degree of tilt, rotates the angle of most advanced and sophisticated magnetic pole or changes the magnetic pole of different degree of tilt, can obtain the non-uniform magnetic-field of different gradients, intensity and direction;
Described replaceable most advanced and sophisticated magnetic pole external member is inserted in the connecting hole of the second magnetic pole fixed bar, relative with described flush end magnetic pole, forms non-homogeneous high-gradient magnetic field Mechanical loading district.
A kind of preferably technical scheme of non-homogeneous high gradient isolated cells of the present utility model magnetic field mechanical loading unit, it is characterized in that described flush end magnetic pole and replaceable most advanced and sophisticated magnetic pole external member, can in the connecting hole of the first magnetic pole fixed bar and the second magnetic pole fixed bar, twitch respectively, thereby adjust the distance between two magnetic poles, to adapt to the isolated cells sample of different capabilities, and change magnetic field rate of loading.
A kind of better technical scheme of non-homogeneous high gradient isolated cells of the present utility model magnetic field mechanical loading unit, described replaceable most advanced and sophisticated magnetic pole external member is the unidirectional tip magnetic pole that one group of degree of tilt is respectively 15 °, 30 °, 45 °, 60 °, 75 ° and 90 °.
A kind of improved technical scheme of non-homogeneous high gradient isolated cells of the present utility model magnetic field mechanical loading unit, characterized by further comprising the first magnetic conduction connecting link and the second magnetic conduction connecting link, the first described magnetic conduction connecting link is positioned at the bottom of the first magnetic conduction sleeve, and the second described magnetic conduction connecting link is positioned at the bottom of the second magnetic conduction sleeve; Be placed in the first magnetic pole fixed bar in the first magnetic conduction sleeve, can be along the first magnetic conduction connecting link sliding extension, be placed in the second magnetic pole fixed bar in the second magnetic conduction sleeve, can be along the second magnetic conduction connecting link sliding extension, thereby adjust described flush end magnetic pole and the center of replaceable most advanced and sophisticated magnetic pole external member, change the length in non-homogeneous high-gradient magnetic field Mechanical loading district.
A kind of further improved technical scheme of non-homogeneous high gradient isolated cells of the present utility model magnetic field mechanical loading unit, it is characterized in that the first described magnetic conduction connecting link and the second magnetic conduction connecting link are connected to one group of height regulating and height adjustment seat, by changing the connection height of height regulating and height adjustment seat, can change the height in non-homogeneous high-gradient magnetic field Mechanical loading district.
The beneficial effects of the utility model are:
1, non-homogeneous high gradient isolated cells of the present utility model magnetic field mechanical loading unit, can carry out magnetic field Mechanical loading to polytype cell (stem cell, tumour cell etc.).
2, non-homogeneous high gradient isolated cells of the present utility model magnetic field mechanical loading unit, by adjust distance between two poles from, adapt to the isolated cells of different capabilities, and change magnetic field rate of loading, the shear stress that isolated cells cytoskeleton is produced to different gradients stimulates.
3, non-homogeneous high gradient isolated cells of the present utility model magnetic field mechanical loading unit, the unidirectional magnetic pole by replacing with certain slope changes magnetic field rate of loading and direction, individual cells different parts cytoskeleton is produced to the shear stress of varying strength and direction, the rationality that stimulates checking cyto-mechanics network to exist by cell being loaded to mechanical stress, observe the deformation that loads cell skeleton, and differentiation due.
Accompanying drawing explanation
Fig. 1 is the structural representation of non-homogeneous high gradient isolated cells of the present utility model magnetic field mechanical loading unit;
Fig. 2 is the front view of non-homogeneous high gradient isolated cells of the present utility model magnetic field mechanical loading unit;
Fig. 3 is the left view of non-homogeneous high gradient isolated cells of the present utility model magnetic field mechanical loading unit.
In figure, 110-magnetic conduction cross bar, 121-the first magnetic conduction sleeve, 122-the second magnetic conduction sleeve, 131-the first magnetic pole fixed bar, 132-the second magnetic pole fixed bar, 141-flush end magnetic pole, the replaceable most advanced and sophisticated magnetic pole external member of 142-, 211-the first magnetic conduction connecting link, 212-the second magnetic conduction connecting link, 220-height regulating, 230-height adjustment seat.
Embodiment
In order to understand better technique scheme of the present utility model, below in conjunction with drawings and Examples, describe in detail further.
An embodiment of non-homogeneous high gradient isolated cells of the present utility model magnetic field mechanical loading unit as shown in Figure 1 to Figure 3, comprise magnetic field mechanics charging assembly, load magnetic pole, for generation of the controllable pulse power supply in magnetic field be connected to the solenoid of controllable pulse power supply, and for detection of the gaussmeter of magnetic field intensity; In the embodiment shown in Fig. 1 to Fig. 3, described magnetic field mechanics charging assembly comprises magnetic conduction cross bar 110, the first magnetic conduction sleeve 121, the second magnetic conduction sleeve 122, the first magnetic pole fixed bar 131, the second magnetic pole fixed bars 132; The first described magnetic conduction sleeve 121 is connected to one end of magnetic conduction cross bar 110 by the first magnetic pole fixed bar 131, the second described magnetic conduction sleeve 122 is connected to the other end of magnetic conduction cross bar 110 by the second magnetic pole fixed bar 132; On the first magnetic conduction sleeve 121, the second magnetic conduction sleeve 122 and/or magnetic conduction cross bar 110, the described solenoid of configuration, forms U-shaped magnetic field mechanics charging assembly;
Described loading magnetic pole is comprised of flush end magnetic pole 141 and one group of replaceable most advanced and sophisticated magnetic pole external member 142; Described flush end magnetic pole 141 inserts in the connecting hole of the first magnetic pole fixed bar 131;
Described replaceable most advanced and sophisticated magnetic pole external member 142 comprises one group of cylindrical unidirectional tip magnetic pole with different degree of tilt α, rotate the angle of most advanced and sophisticated magnetic pole or change the magnetic pole of different degree of tilt α, can obtain the non-uniform magnetic-field of different gradients, intensity and direction, individual cells different parts cytoskeleton be produced to the shear stress of varying strength and direction.
Described replaceable most advanced and sophisticated magnetic pole external member 142 is inserted in the connecting hole of the second magnetic pole fixed bar 132, relative with described flush end magnetic pole 141, forms non-homogeneous high-gradient magnetic field Mechanical loading district.
Described flush end magnetic pole 141 and replaceable most advanced and sophisticated magnetic pole external member 142, can in the connecting hole of the first magnetic pole fixed bar 131 and the second magnetic pole fixed bar 132, twitch respectively, thereby the distance S(between adjustment two magnetic poles is referring to Fig. 2), to adapt to the isolated cells of different capabilities, and changing magnetic field rate of loading, the shear stress that isolated cells cytoskeleton is produced to different gradients stimulates.
Described replaceable most advanced and sophisticated magnetic pole external member 142 is unidirectional tip magnetic poles that one group of degree of tilt α is respectively 15 °, 30 °, 45 °, 60 °, 75 ° and 90 °.
According to the embodiment shown in Fig. 1 and Fig. 3, non-homogeneous high gradient isolated cells of the present utility model magnetic field mechanical loading unit also comprises the first magnetic conduction connecting link 211 and the second magnetic conduction connecting link 212, the first described magnetic conduction connecting link 211 is positioned at the bottom of the first magnetic conduction sleeve 121, and the second described magnetic conduction connecting link 212 is positioned at the bottom of the second magnetic conduction sleeve 122; Be placed in the first magnetic pole fixed bar 131 in the first magnetic conduction sleeve 121, can be along the first magnetic conduction connecting link 211 sliding extensions, be placed in the second magnetic pole fixed bar 132 in the second magnetic conduction sleeve 122, can be along the second magnetic conduction connecting link 212 sliding extensions, thereby adjust described flush end magnetic pole 141 and the center of replaceable most advanced and sophisticated magnetic pole external member 142, change the length L in non-homogeneous high-gradient magnetic field Mechanical loading district, referring to Fig. 3.
According to the embodiment of the of the present utility model non-homogeneous high gradient isolated cells magnetic field mechanical loading unit shown in Fig. 1 to Fig. 3, the first described magnetic conduction connecting link 211 and the second magnetic conduction connecting link 212 are connected to one group of height regulating 220 and height adjustment seat 230, by changing the connection height of height regulating 220 and height adjustment seat 230, can change the height H in non-homogeneous high-gradient magnetic field Mechanical loading district, referring to Fig. 2.
Non-homogeneous high gradient isolated cells of the present utility model magnetic field mechanical loading unit operating process is as follows:
1, according to the model of microscope or operating platform and sample volume, regulate the magnetic field mechanics charging assembly of non-homogeneous high gradient isolated cells of the present utility model magnetic field mechanical loading unit, change length L and the height H in non-homogeneous high-gradient magnetic field Mechanical loading district;
2, non-homogeneous high gradient isolated cells of the present utility model magnetic field mechanical loading unit is connected to microscope or operating platform;
3, Tissue Culture Dish is placed between flush end magnetic pole 141 and replaceable most advanced and sophisticated magnetic pole external member 142;
4, open controllable pulse power generation magnetic field;
5, regulate distance between two poles to change magnetic field intensity from S, cell is carried out to magnetic force loading;
6, change the most advanced and sophisticated magnetic pole of suitable degree of tilt α, produce the non-homogeneous high-gradient magnetic field that meets sample requirement;
7, use gaussmeter to detect magnetic field intensity, acquisition and recording experimental data;
8, Data Collection and analysis.
Those of ordinary skill in the art will be appreciated that; above embodiment is only for the technical solution of the utility model is described; and be not used as restriction of the present utility model; any variation of the above embodiment being done based on connotation of the present utility model, modification, all will drop in the protection domain of claim of the present utility model.
Claims (5)
1. a non-homogeneous high gradient isolated cells magnetic field mechanical loading unit, comprise magnetic field mechanics charging assembly, load magnetic pole, for generation of controllable pulse power supply and the solenoid that is connected to controllable pulse power supply in magnetic field, and for detection of the gaussmeter of magnetic field intensity, it is characterized in that:
Described magnetic field mechanics charging assembly comprises magnetic conduction cross bar, the first magnetic conduction sleeve, the second magnetic conduction sleeve, the first magnetic pole fixed bar, the second magnetic pole fixed bar; The first described magnetic conduction sleeve is connected to one end of magnetic conduction cross bar by the first magnetic pole fixed bar, the second described magnetic conduction sleeve is connected to the other end of magnetic conduction cross bar by the second magnetic pole fixed bar; On the first magnetic conduction sleeve, the second magnetic conduction sleeve and/or magnetic conduction cross bar, the described solenoid of configuration, forms U-shaped magnetic field mechanics charging assembly;
Described loading magnetic pole is comprised of flush end magnetic pole and one group of replaceable most advanced and sophisticated magnetic pole external member; Described flush end magnetic pole inserts in the connecting hole of the first magnetic pole fixed bar;
Described replaceable most advanced and sophisticated magnetic pole external member comprises one group of cylindrical unidirectional tip magnetic pole with different degree of tilt, rotates the angle of most advanced and sophisticated magnetic pole or changes the magnetic pole of different degree of tilt, can obtain the non-uniform magnetic-field of different gradients, intensity and direction;
Described replaceable most advanced and sophisticated magnetic pole external member is inserted in the connecting hole of the second magnetic pole fixed bar, relative with described flush end magnetic pole, forms non-homogeneous high-gradient magnetic field Mechanical loading district.
2. non-homogeneous high gradient isolated cells according to claim 1 magnetic field mechanical loading unit, it is characterized in that described flush end magnetic pole and replaceable most advanced and sophisticated magnetic pole external member, can in the connecting hole of the first magnetic pole fixed bar and the second magnetic pole fixed bar, twitch respectively, thereby adjust the distance between two magnetic poles, to adapt to the isolated cells sample of different capabilities, and change magnetic field rate of loading.
3. non-homogeneous high gradient isolated cells according to claim 1 magnetic field mechanical loading unit, described replaceable most advanced and sophisticated magnetic pole external member is the unidirectional tip magnetic pole that one group of degree of tilt is respectively 15 °, 30 °, 45 °, 60 °, 75 ° and 90 °.
4. according to the non-homogeneous high gradient isolated cells magnetic field mechanical loading unit described in claim 1,2 or 3, characterized by further comprising the first magnetic conduction connecting link and the second magnetic conduction connecting link, the first described magnetic conduction connecting link is positioned at the bottom of the first magnetic conduction sleeve, and the second described magnetic conduction connecting link is positioned at the bottom of the second magnetic conduction sleeve; Be placed in the first magnetic pole fixed bar in the first magnetic conduction sleeve, can be along the first magnetic conduction connecting link sliding extension, be placed in the second magnetic pole fixed bar in the second magnetic conduction sleeve, can be along the second magnetic conduction connecting link sliding extension, thereby adjust described flush end magnetic pole and the center of replaceable most advanced and sophisticated magnetic pole external member, change the length in non-homogeneous high-gradient magnetic field Mechanical loading district.
5. non-homogeneous high gradient isolated cells according to claim 4 magnetic field mechanical loading unit, it is characterized in that the first described magnetic conduction connecting link and the second magnetic conduction connecting link are connected to one group of height regulating and height adjustment seat, by changing the connection height of height regulating and height adjustment seat, can change the height in non-homogeneous high-gradient magnetic field Mechanical loading district.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201320620751.4U CN203520223U (en) | 2013-10-09 | 2013-10-09 | Non-uniform high-gradient isolated-cell magnetic-field mechanics loading device |
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| CN201320620751.4U CN203520223U (en) | 2013-10-09 | 2013-10-09 | Non-uniform high-gradient isolated-cell magnetic-field mechanics loading device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104560708A (en) * | 2013-10-09 | 2015-04-29 | 上海市同济医院 | Non-uniform high-gradient isolated-cell magnetic-field mechanics loading device |
| CN108724148A (en) * | 2018-09-17 | 2018-11-02 | 湖南早晨纳米机器人有限公司 | Nanometer robot control system |
-
2013
- 2013-10-09 CN CN201320620751.4U patent/CN203520223U/en not_active Withdrawn - After Issue
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104560708A (en) * | 2013-10-09 | 2015-04-29 | 上海市同济医院 | Non-uniform high-gradient isolated-cell magnetic-field mechanics loading device |
| CN108724148A (en) * | 2018-09-17 | 2018-11-02 | 湖南早晨纳米机器人有限公司 | Nanometer robot control system |
| CN108724148B (en) * | 2018-09-17 | 2019-01-01 | 湖南早晨纳米机器人有限公司 | Nanometer robot control system |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20140402 Effective date of abandoning: 20160810 |
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| C25 | Abandonment of patent right or utility model to avoid double patenting |