CN203405366U - Simple booster for prepacked column protein purification - Google Patents
Simple booster for prepacked column protein purification Download PDFInfo
- Publication number
- CN203405366U CN203405366U CN201320551601.2U CN201320551601U CN203405366U CN 203405366 U CN203405366 U CN 203405366U CN 201320551601 U CN201320551601 U CN 201320551601U CN 203405366 U CN203405366 U CN 203405366U
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- China
- Prior art keywords
- sliding
- prepacked column
- stone roller
- booster
- sliding block
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- 238000001742 protein purification Methods 0.000 title abstract description 10
- 241001504664 Crossocheilus latius Species 0.000 claims description 42
- 108090000623 proteins and genes Proteins 0.000 claims description 24
- 102000004169 proteins and genes Human genes 0.000 claims description 24
- 230000001965 increasing effect Effects 0.000 claims description 20
- 229910001018 Cast iron Inorganic materials 0.000 claims description 6
- 229910001220 stainless steel Inorganic materials 0.000 claims description 6
- 239000010935 stainless steel Substances 0.000 claims description 6
- 239000010453 quartz Substances 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 230000005484 gravity Effects 0.000 abstract description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 18
- 239000007788 liquid Substances 0.000 description 11
- 239000002699 waste material Substances 0.000 description 10
- 229910052742 iron Inorganic materials 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 7
- 238000000746 purification Methods 0.000 description 6
- 238000012913 prioritisation Methods 0.000 description 5
- 229910000754 Wrought iron Inorganic materials 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 101710120037 Toxin CcdB Proteins 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000001042 affinity chromatography Methods 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000013016 damping Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 229910001651 emery Inorganic materials 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000010977 jade Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 description 1
- NTTIDCCSYIDANP-UHFFFAOYSA-N BCCP Chemical compound BCCP NTTIDCCSYIDANP-UHFFFAOYSA-N 0.000 description 1
- 101710201279 Biotin carboxyl carrier protein Proteins 0.000 description 1
- 101710180532 Biotin carboxyl carrier protein of acetyl-CoA carboxylase Proteins 0.000 description 1
- 239000000910 agglutinin Substances 0.000 description 1
- 238000011091 antibody purification Methods 0.000 description 1
- 230000002146 bilateral effect Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000002532 enzyme inhibitor Substances 0.000 description 1
- 229940125532 enzyme inhibitor Drugs 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
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- 229960005486 vaccine Drugs 0.000 description 1
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Images
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- Peptides Or Proteins (AREA)
Abstract
The utility model discloses a simple booster for prepacked column protein purification. The simple booster is composed of a base, sliding rods, slide knots, a sliding block, a cross rod, sliding sleeves, an increment weight loop bar and increment weights, wherein the base is placed on a smooth table top; the sliding rods are connected with the base through threaded holes; the slide knots are fixedly connected to the sliding rods, and the positions, located at the sliding rods, of the slide knots are same; the cross rod is sleeved with the sliding block, and an injector push rod round groove is formed in the lower portion of the sliding block; the sliding block and the sliding sleeves are connected through the cross rod, and the cross rod, the sliding block and the sliding sleeves are fixed together to form a sliding frame; the sliding sleeves are connected to the sliding rods; the increment weight loop bar is fixed to the sliding block, and the increment weights are arranged on the increment weight loop bar. An injector is pressurized through the gravity of the sliding block and the gravity of the increment weights to clean a prepacked column, the speed is low, overpressure is avoided, the prepacked column is protected, and damage to padding in the prepacked column is small. The simple booster is small, reasonable and convenient to move. The sliding block has the function of a handle, so that lifting is facilitated. The injector piston push rod round groove has the advantages of being safe in operation, stable and reliable.
Description
Technical field
The utility model belongs to biology experimental installation field, relates in particular to a kind of simple and easy booster for prepacked column purifying protein.
Background technology
The separation and purification of protein is to use an important operation technology very widely in biochemical research application.And the purity of protein isolate directly affects it in further investigation and the application of the every field such as clinical medicine, pharmacology, immunology and Food Chemistry.Along with the fast development of each subject, people constantly increase the demand of protein purification technology in recent years, and existing purification process is improved day by day, and novel purification process also emerges in large numbers in succession.According to ligand specificity, as: enzyme-substrate, enzyme-inhibitor, glycoprotein-agglutinin, Ag-Ab etc., people have been developed affinity chromatography (Affinity chromatography) protein isolate.The method is very effective, especially easy with the various label purification process of development in recent years, conventionally only need process and can make certain protein to be purified separate from complicated protein mixture through a step, and purity is very high.In biological experiment, New-type label and corresponding purification system mainly contain GST label, His label, Flag label, CBP label, BCCP label, MBP label and for Protein G, the Protein A etc. of monoclonal antibody purifying; Each biotech firm provides various corresponding affine resins to get final product purifying destination protein simultaneously.Commercially available affinity chromatograph filling is as of many uses in normal experiment in GST, His, Protein G etc., corresponding product also provides the prepacked column of different size, in conjunction with protein liquid chromatography system AKTA separated special target proteins fast and effeciently, be especially applicable to the micropreparation that albumen is carried out in laboratory; The work such as scientific research, bio-pharmaceuticals, vaccine, diagnostic reagent development are all had very great help.But AKTA protein purification system is expensive, most of laboratories, due to the impact of scale and funds, seldom can be equipped with supporting instrument for experiment.
Therefore operator utilizes syringe to carry out manual operations conventionally, but the outstanding problem that manual operations exists is operation inconvenience, wastes time and energy and inefficiency etc.The HiTrap Protein G HP prepacked column monoclonal antibody purification of 1mL specification of take is example, complete antibody Sample Purification on Single 1 time, from prepacked column balance, loading, foreign protein clean, antibody elution is to prepacked column balance again, completes 50～80mL liquid dropwise by hand, at least needs 1.5 hours; The general antibody amount that meets common requirement of experiment that obtains, needs repetitive operation 3～5 times.This process not only operates effort, and serious waste scientific research personnel's quality time.Moreover existing AKTA protein purification system is expensive, and syringe when manual operations operation inconvenience, waste time and energy and inefficiency.Given this, the utility model provides a kind of simple and easy booster that is suitable for prepacked column purifying protein to overcome the problem of above-mentioned difficulties and existence, to improving experimenter's work efficiency.In addition, domestic existing patent is searched in element, about the simple and easy power assisting device of protein purification, has not yet inquired similar patent or product.
Utility model content
The purpose of this utility model is to provide a kind of simple and easy booster that is suitable for prepacked column purifying protein, is intended to solve AKTA protein purification system expensive, and syringe when manual operations operation inconvenience, wastes time and energy and the problem of inefficiency.
The utility model is achieved in that a kind of simple and easy booster that is suitable for prepacked column purifying protein, and its structure is comprised of base, slide bar, back hitch, sliding shoe, cross bar, sliding sleeve, increasing stone roller loop bar and increasing stone roller.
Base is placed on smooth table top, for fixing and support simple and easy booster;
Slide bar is connected with base by threaded hole, for the slip of sliding shoe, forms two slide bar iron stands with base;
Back hitch is fixed in the isometry position place of slide bar, for supporting travelling carriage;
Sliding shoe is enclosed within on cross bar, for supporting, increases stone roller and increases stone roller loop bar;
Below sliding shoe, be provided with injector push-rod circular groove, for stable injectable device push rod;
Cross bar connects sliding shoe and sliding sleeve, with sliding shoe and the sliding sleeve formation travelling carriage that is fixed together;
Sliding sleeve is connected on slide bar, for sliding up and down of travelling carriage;
Increase stone roller loop bar and be fixed on sliding shoe, for the fixing stone roller that increases;
Increasing stone roller is added on increasing stone roller loop bar, for travelling carriage being applied to different power.
Further, base material is quartz, is shaped as rectangle; Slide bar, increasing stone roller loop bar, travelling carriage are stainless steel, and being shaped as of slide bar is cylindrical;
Further, slide bar is symmetrical 2; 1 of sliding shoe, deadweight 500g～1000g; Increase 1 of stone roller loop bar; Increase 1～4 of stone roller, 250g/ is cast iron quality;
Further, injector push-rod circular groove is circular, diameter 3.2cm.
A kind of simple and easy booster for prepacked column purifying protein that the utility model provides adopts quartz and stainless steel, cheap; And anticorrosion power is strong, durable in use, be easy to clean.By slide block and the gravity that increases stone roller, the syringe prepacked column that pressurize is cleaned, speed relaxes, and avoids overvoltage, protects prepacked column, simultaneously little to filler destructive power in prepacked column.This device is small and exquisite rationally, is convenient to move, and allows experimenter to take into account other experimental implementation, can save the protein purification working time of 70% left and right; Simple Design, sliding shoe has handle function concurrently, and weight is suitable, conveniently lifts.Syringe piston push rod circular groove is applicable to fix 1～50mL different size syringe, has handling safety, reliable and stable feature.This booster is equipped with 1～4 cast iron to increase stone roller, and 250g/, can be according to experiment demand, arbitrarily combination, coutroi velocity.Use more convenient, time saving and energy saving, the visual design is good.Booster cost is inexpensive, is easy to accept into common lab.
Accompanying drawing explanation
Fig. 1 is the structural drawing of the simple and easy booster that is suitable for prepacked column purifying protein that provides of the utility model;
Fig. 2 is that the simple and easy booster cast iron that the utility model provides increases the structural drawing of cutting or polish jade with an emery wheel;
Fig. 3 is the structural drawing of the simple and easy booster travelling carriage that provides of the utility model;
The structural drawing of the simple and easy booster specific embodiment that Fig. 4 provides for the utility model.
In figure: 1, iron stand base; 2, iron stand slide bar; 3, back hitch; 4, sliding shoe; 5, travelling carriage cross bar; 6, sliding sleeve; 7, increase stone roller loop bar; 8, increase stone roller; 9, circular groove; 10, syringe; 11,0.45 μ m filter; 12, prepacked column; 13, waste liquid bottle.
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with embodiment, the utility model is further elaborated.Should be appreciated that specific embodiment described herein is only in order to explain the utility model, and be not used in restriction the utility model.
Fig. 1 shows the simple and easy structure of actuator that is suitable for prepacked column purifying protein that the utility model embodiment provides.For convenience of explanation, only show the part relevant to the utility model.
The simple and easy booster that is suitable for prepacked column purifying protein of the utility model embodiment, its structure is comprised of base, slide bar, back hitch, sliding shoe, cross bar, sliding sleeve, increasing stone roller loop bar and increasing stone roller.
Base is placed on smooth table top, for fixing and support simple and easy booster;
Slide bar is connected with base by threaded hole, for the slip of sliding shoe, forms two slide bar iron stands with base;
Back hitch is fixed in the isometry position place of slide bar, for supporting travelling carriage;
Sliding shoe is enclosed within on cross bar, for supporting, increases stone roller and increases stone roller loop bar;
Below sliding shoe, be provided with injector push-rod circular groove, for stable injectable device push rod;
Cross bar connects sliding shoe and sliding sleeve, with sliding shoe and the sliding sleeve formation travelling carriage that is fixed together;
Sliding sleeve is connected on slide bar, for sliding up and down of travelling carriage;
Increase stone roller loop bar and be fixed on sliding shoe, for the fixing stone roller that increases;
Increasing stone roller is added on increasing stone roller loop bar, for travelling carriage being applied to different power.
As a prioritization scheme of the utility model embodiment, base material is quartz, is shaped as rectangle; Slide bar, increasing stone roller loop bar, travelling carriage are stainless steel, and being shaped as of slide bar is cylindrical;
As a prioritization scheme of the utility model embodiment, slide bar is symmetrical 2; 1 of sliding shoe, deadweight 500g～1000g; Increase 1 of stone roller loop bar; Increase 1～4 of stone roller, 250g/ is cast iron quality;
As a prioritization scheme of the utility model embodiment, injector push-rod circular groove is circular, diameter 3.2cm;
As a prioritization scheme of the utility model embodiment, specific embodiments 1 is configuration use 1mL prepacked column, 0.45 μ m filter, and increasing stone roller is 1, specification of syringe is 20mL, 1 of waste liquid bottle;
As a prioritization scheme of the utility model embodiment, 5mL prepacked column is used in specific embodiments 2 configurations, 0.45 μ m filter, and increasing stone roller is 2～4, specification of syringe is 50mL, 1 of waste liquid bottle.
First according to experiment demand, simple and easy booster is combined into and has 1～4 device that increases stone roller and different size prepacked column and syringe, this device balance is placed in to table top.The syringe that is filled with damping fluid is connected to 0.45 μ m filter and protein purification prepacked column successively by joint, under connect waste liquid bottle.Upper sliding moving frame, is fixed on injector push-rod in sliding shoe groove, places steadily, makes it with gravity, naturally decline and carry out post cleaning; Treat that liquid flows to end, take off syringe and be again filled liquid, repeatedly clean.After albumen collection and cylinder clean and finish, take out prepacked column and preserve by its instructions requirement.After clean booster, be placed on ventilation, to use next time.
Below in conjunction with drawings and Examples, the utility model is described further.
Embodiment 1:
Simple and easy booster as shown in Fig. 1～4, includes two slide bar iron stands, travelling carriage, increasing stone roller 8 and increases stone roller loop bar 7; Two slide bar iron stands are comprised of iron stand base 1 and iron stand slide bar 2; At the bottom of described iron stand
Seat 1 is rectangle, and iron stand slide bar 2 is cylindrical; Bilateral iron stand slide bar 2 arranges back hitch 3 apart from iron stand base 1 equal-height position, in order to support travelling carriage; Sliding shoe 4 is by travelling carriage cross bar 5 and the sliding sleeve 6 formation travelling carriage that is fixed together, and is connected in iron stand slide bar 2 by sliding sleeve 6, can slide by easy on and off; The sliding shoe 4 affixed increasings in top stone roller loop bars 7, cylindrical, in order to fixing cast iron, increase and cut or polish jade with an emery wheel 8; Be provided with circular groove 9 in the middle of sliding shoe bottom surface, in order to the fixing piston push rod of syringe 10.
Embodiment 2:
As shown in Figure 4, on embodiment 1 basis, 1mL prepacked column is used in configuration, 0.45 μ m filter, and increasing stone roller is 1, specification of syringe is 20mL, 1 of waste liquid bottle.
Embodiment 3:
Substantially the same manner as Example 2, difference is to use 5mL prepacked column, and increasing stone roller is 2～4, and syringe is 50mL.
Concrete implementation of the present utility model is carried out according to the following procedure: first according to experiment demand, simple and easy booster is combined into and has 1～4 device that increases stone roller and different size prepacked column and syringe, this device balance is placed in to table top.The syringe that is filled with damping fluid is connected to 0.45 μ m filter and protein purification prepacked column successively by joint, under connect waste liquid bottle.Upper sliding moving frame, is fixed on injector push-rod in sliding shoe groove, places steadily, makes it with gravity, naturally decline and carry out post cleaning; Treat that liquid flows to end, take off syringe and be again filled liquid, repeatedly clean.After albumen collection and cylinder clean and finish, take out prepacked column and preserve by its instructions requirement.After clean booster, be placed on ventilation, to use next time.
The foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; all any modifications of doing within spirit of the present utility model and principle, be equal to and replace and improvement etc., within all should being included in protection domain of the present utility model.
Claims (5)
1. a simple and easy booster that is suitable for prepacked column purifying protein, is characterized in that, described simple and easy booster comprises base, slide bar, back hitch, sliding shoe, cross bar, sliding sleeve, increasing stone roller loop bar and increases stone roller;
Base is arranged on smooth table top; Slide bar is connected with base by threaded hole, and back hitch is fixed on slide bar; Sliding shoe is enclosed within on cross bar, is provided with injector push-rod circular groove below; Cross bar connects sliding shoe and sliding sleeve, and sliding sleeve is connected on slide bar; Increasing stone roller loop bar is fixed on sliding shoe; Increasing stone roller is arranged on increasing stone roller loop bar.
2. the simple and easy booster that is suitable for prepacked column purifying protein as claimed in claim 1, is characterized in that, this simple and easy booster adopts quartz base.
3. the simple and easy booster that is suitable for prepacked column purifying protein as claimed in claim 1, is characterized in that, this simple and easy booster adopts stainless steel slide bar, stainless steel to increase stone roller loop bar, stainless steel travelling carriage.
4. the simple and easy booster that is suitable for prepacked column purifying protein as claimed in claim 1, is characterized in that, this simple and easy booster adopts cast iron to increase stone roller.
5. the simple and easy booster that is suitable for prepacked column purifying protein as claimed in claim 1, is characterized in that, injector push-rod circular groove is set to the circle of diameter 3.2cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320551601.2U CN203405366U (en) | 2013-09-06 | 2013-09-06 | Simple booster for prepacked column protein purification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320551601.2U CN203405366U (en) | 2013-09-06 | 2013-09-06 | Simple booster for prepacked column protein purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203405366U true CN203405366U (en) | 2014-01-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320551601.2U Expired - Fee Related CN203405366U (en) | 2013-09-06 | 2013-09-06 | Simple booster for prepacked column protein purification |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203405366U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107903298A (en) * | 2017-12-27 | 2018-04-13 | 湖北普罗金科技有限公司 | A kind of liftable pressurization gravity column for protein purification |
-
2013
- 2013-09-06 CN CN201320551601.2U patent/CN203405366U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107903298A (en) * | 2017-12-27 | 2018-04-13 | 湖北普罗金科技有限公司 | A kind of liftable pressurization gravity column for protein purification |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140122 |