CN203376317U - Lactam detection kit - Google Patents
Lactam detection kit Download PDFInfo
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- CN203376317U CN203376317U CN201320541330.2U CN201320541330U CN203376317U CN 203376317 U CN203376317 U CN 203376317U CN 201320541330 U CN201320541330 U CN 201320541330U CN 203376317 U CN203376317 U CN 203376317U
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Abstract
A lactam detection kit comprises an elisa plate storage groove (7) and a plastic support (8). The lactam detection kit is characterized in that the elisa plate storage groove (7) is located in the lower portion of the plastic support (8). The plastic support (8) is provided with a standard substance solution area (1), a penicillin-binding protein 6 solution hole site (2), an antibody solution hole site (3), a chromogenic substrate liquid hole site (4), a stop buffer hole site (5) and a concentration scrubbing solution hole site (6). Six standard substance solution hole sites (1-1) which are arranged in two rows and three lines are formed inside the standard substance solution area (1). The lactam detection kit has the advantages of being high in detection sensitivity, easy and convenient to operate, high in detecting speed, capable of being used for large-scale screening detection of beta-lactam antibiotics in various foods and very suitable for substratum detection.
Description
Technical field
The present invention relates to field of biological detection, be specially a kind of lactams detection kit.
Background technology
Beta-lactam antibiotic (β-Lactam antibiotics) is the microbiotic that a large class contains the beta-lactam ring structure, mainly comprise penicillins, cephalosporins, cephamycin-type, Carbapenems and monobactams etc., wherein penicillins and cephalosporins are with the fastest developing speed, are most widely used.Beta-lactam medicine is mainly by suppressing the glutinous peptide synthetase of cell membrane, and PBP (Penicillin binding proteins, PBPs) activity, stop the synthetic of whole cell peptidoglycan, makes the bacterium thalline break and produce antibacterial action.
Because such medicine effect is outstanding, also there are some problems while applying aborning, such as drug abuse, not according to off-drug period drug withdrawal, the random compatibility of different pharmaceutical etc., cause curative effect of medication to reduce, also relevant animal food safety has been caused to hidden danger.The Beta-lactam medicine problem of maximum at present is exactly to cause that part is not suitable with crowd's allergic reaction, causes the impaired even death of health etc., and such as carcinogenic, teratogenesis, mutagenesis etc. also studies have reported that in addition.
Owing to there being various potential hazards, at present in world wide all to Beta-lactam medicine the residue limits in animal derived food done strict regulations, wherein European Union, the U.S., Japan and Chinese limiting the quantity of require substantially similar, as the requirement great majority to penicillin medicine at 4 μ g/L.And the existing detection method majority of Beta-lactam medicine take instrumental method as gas chromatography and gas chromatography mass spectrometry method, high performance liquid chromatography and Liquid Chromatography/Mass Spectrometry and capillary electrophoresis etc. as main, screening technique be take immunoassay as main.By contrast, method for quick and the means that can in basic unit is detected, apply are few.
Summary of the invention
The application's purpose is to provide a kind of lactams detection kit, can not quick and precisely detect the problem of 12 kinds of beta-lactam antibiotics that comprise 7 kinds of penicillin and 5 kinds of Cephalosporins to solve the existing test strips product of kit.
In order to achieve the above object, the present invention is by the following technical solutions:
A kind of lactams detection kit, comprise ELISA Plate storage tank (7) and plastics holder (8), it is characterized in that: described ELISA Plate storage tank (7) is positioned at the below of plastics holder (8), described plastics holder (8) is provided with standard solution district (1), PBP 6 positions, solution hole (2), position, antibody-solutions hole (3), position, chromogenic substrate liquid hole (4), position, stop buffer hole (5) and position, concentrated cleaning solution hole (6), be provided with two row's three row totally six positions, standard mouth product solution hole (1-1) in described standard solution district (1), position, described concentrated cleaning solution hole (6) is positioned at the leftmost side of plastics holder (8), described PBP 6 positions, solution hole (2), position, antibody-solutions hole (3), position, chromogenic substrate liquid hole (4) and position, stop buffer hole (5) are positioned between standard solution district (1) and position, concentrated cleaning solution hole (6).
What further, in described standard solution district (1), place is the ampicillin standard items that six kinds of variable concentrations are housed, the Brown Glass Brown glass bottles and jars only that capacity is 2ml.
Further, described PBP 6 positions, solution hole (2) are the positions, hole that capacity is the 7ml plastic bottle.
Further, position, described antibody-solutions hole (3) are the positions, hole that capacity is the 7ml Brown Glass Brown glass bottles and jars only.
Further, position, described stop buffer hole (5) are the positions, hole that capacity is the 7ml plastic bottle.
What further, in position, described concentrated cleaning solution hole (6), deposit is the plastic bottle of 50ml.
Further, also be placed with batch report and kit operation instructions in described lactams detection kit, integral body is put into external packing box.
Beneficial effect of the present invention is: detection sensitivity is high, easy and simple to handle, and detection speed is fast, easy to operate, and the Large-scale Screening that can be used for the beta-lactam antibiotic in varieties of food items detects, and is very suitable for basic unit and detects use.
The accompanying drawing explanation
Fig. 1 the utility model reagent constituents and the distribution plan in box thereof
Standard solution district 1; Standard mouth product solution hole position 1-1; PBP 6 positions, solution hole 2; Position, antibody-solutions hole 3; Position, chromogenic substrate liquid hole 4; Position, stop buffer hole 5; Position, concentrated cleaning solution hole 6; ELISA Plate storage tank 7; Plastics holder 8
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the utility model is further elaborated.Should be appreciated that specific embodiment described herein is only in order to explain the utility model, and be not used in restriction the utility model.
The utility model comprises plastics holder (8), being provided with seven functional areas in plastics holder (8), is respectively standard solution district (1), PBP 6 positions, solution hole (2), position, antibody-solutions hole (3), position, chromogenic substrate liquid hole (4), position, stop buffer hole (5), position, concentrated cleaning solution hole (6) and ELISA Plate storage tank (7).
Totally six storerooms, position, two rows standard mouth product solution hole (1-1) wherein are set in standard solution district (1), be provided with PBP 6 positions, solution hole (2), position, antibody-solutions hole (3), position, chromogenic substrate liquid hole (4) and position, stop buffer hole (5) between standard solution district (1) and position, concentrated cleaning solution hole (6), position, concentrated cleaning solution hole (6) is because use amount is other reagent several times, therefore, other storerooms of Area Ratio of position, concentrated cleaning solution hole (6) all want large.
What the PBP 6 interior correspondences in position, solution hole (2) were deposited is PBP 6 solution, what in position, antibody-solutions hole (3), deposit is PBP 6 monoclonal antibody solutions of HRP mark, position, chromogenic substrate liquid hole (4) is used for settling one-component TMB chromogenic substrate liquid, the position, hole that position, stop buffer hole (5) is stop buffer, stop buffer is the sulfuric acid solution of 1M, position, concentrated cleaning solution hole (6) is 20 times of concentrated cleaning solution storage areas, ELISA Plate storage tank (7) is vacuum-packed ELISA Plate, it is rectangle, be positioned under each position, hole, ELISA Plate storage tank (7) is the vacuum bag encapsulation, be provided with dryer damp-proofing in it, after standard solution district (1) seals with vial, that in standard solution district (1), deposit is pH7.4,0.1MPBS dissolved ammonia parasiticin standard items, and dilution is gradient concentration 0,0.05,0.2,0.8,2.4 and 9.6 μ g/L to deposit in the 2ml Brown Glass Brown glass bottles and jars only standby, reduce the destruction of illumination to internal solution by using Brown Glass Brown glass bottles and jars only, extend the shelf life.Each concentration in standard solution district (1) is all placed 1 bottle in kit; PBP 6 positions, solution hole (2) are position, 7ml hole, and what in it, deposit is plastic bottle; What placed position, antibody-solutions hole (3) is the Brown Glass Brown glass bottles and jars only of 7ml; What in position, stop buffer hole (5), place is the 7ml plastic bottle; What in position, concentrated cleaning solution hole (6), deposit is the plastic bottle of 50ml.During the kit assembling, above-mentioned each component is deposited according to the method for Fig. 1, also be placed with batch report and kit operation instructions in the lactams detection kit, integral body is put into external packing box.
The foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; the material that the utility model relates to is known materials; all any modifications of doing within spirit of the present utility model and principle, be equal to and replace and improvement etc., within all should being included in protection domain of the present utility model.
Claims (7)
1. a lactams detection kit, comprise ELISA Plate storage tank (7) and plastics holder (8), it is characterized in that: described ELISA Plate storage tank (7) is positioned at the below of plastics holder (8), described plastics holder (8) is provided with standard solution district (1), PBP 6 positions, solution hole (2), position, antibody-solutions hole (3), position, chromogenic substrate liquid hole (4), position, stop buffer hole (5) and position, concentrated cleaning solution hole (6), be provided with two row's three row totally six positions, standard mouth product solution hole (1-1) in described standard solution district (1), position, described concentrated cleaning solution hole (6) is positioned at the leftmost side of plastics holder (8), described PBP 6 positions, solution hole (2), position, antibody-solutions hole (3), position, chromogenic substrate liquid hole (4) and position, stop buffer hole (5) are positioned between standard solution district (1) and position, concentrated cleaning solution hole (6).
2. a kind of lactams detection kit according to claim 1 is characterized in that: what in described standard solution district (1), place is the ampicillin standard items that six kinds of variable concentrations are housed, the Brown Glass Brown glass bottles and jars only that capacity is 2ml.
3. a kind of lactams detection kit according to claim 2, it is characterized in that: described PBP 6 positions, solution hole (2) are the positions, hole that capacity is the 7ml plastic bottle.
4. a kind of lactams detection kit according to claim 3, it is characterized in that: position, described antibody-solutions hole (3) is the position, hole that capacity is the 7ml Brown Glass Brown glass bottles and jars only.
5. a kind of lactams detection kit according to claim 4, it is characterized in that: position, described stop buffer hole (5) is the position, hole that capacity is the 7ml plastic bottle.
6. a kind of lactams detection kit according to claim 5, it is characterized in that: what in position, described concentrated cleaning solution hole (6), deposit is the plastic bottle of 50ml.
7. according to the arbitrary described a kind of lactams detection kit of claim 1-6, it is characterized in that: also be placed with batch report and kit operation instructions in described lactams detection kit, integral body is put into external packing box.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320541330.2U CN203376317U (en) | 2013-09-02 | 2013-09-02 | Lactam detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320541330.2U CN203376317U (en) | 2013-09-02 | 2013-09-02 | Lactam detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203376317U true CN203376317U (en) | 2014-01-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320541330.2U Expired - Fee Related CN203376317U (en) | 2013-09-02 | 2013-09-02 | Lactam detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203376317U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104215775A (en) * | 2014-09-04 | 2014-12-17 | 北京纳百景弈生物科技有限公司 | Method for preparing detection kit based on penicillin-binding protein PBP6 beta-lactam antibiotic receptor assay and detection method thereof |
-
2013
- 2013-09-02 CN CN201320541330.2U patent/CN203376317U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104215775A (en) * | 2014-09-04 | 2014-12-17 | 北京纳百景弈生物科技有限公司 | Method for preparing detection kit based on penicillin-binding protein PBP6 beta-lactam antibiotic receptor assay and detection method thereof |
| CN104215775B (en) * | 2014-09-04 | 2016-01-20 | 北京纳百景弈生物科技有限公司 | The beta-lactam antibiotic receptor method detection kit preparation method of PBP PBP6 and detection method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140101 Termination date: 20200902 |
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| CF01 | Termination of patent right due to non-payment of annual fee |