CN203360445U

CN203360445U - Ultrasound microbubble mediated transfection auxiliary device

Info

Publication number: CN203360445U
Application number: CN 201320378886
Authority: CN
Inventors: 李东野; 蒋学友; 陈丹; 王晓萍; 朱红; 刘洋; 陈军红; 夏勇
Original assignee: Xuzhou Medical College
Current assignee: Xuzhou Medical College
Priority date: 2013-06-27
Filing date: 2013-06-27
Publication date: 2013-12-25
Anticipated expiration: 2023-06-27

Abstract

The utility model discloses an ultrasound microbubble mediated transfection auxiliary device, which includes an opened big bottle engraved with scale grooves at the upper end, a pair of adjusting iron wires, a pair of bearing iron wires and a culture dish placed on the bearing iron wires, wherein the bearing iron wires are placed inside the big bottle in an overlapping manner; the adjusting iron wires are positioned under the bearing iron wires and placed on the scale grooves in an overlapping manner; the scale grooves are spaced at an interval of 0.5 cm; the pair of adjusting iron wires and the pair of bearing iron wires are the same in shape, are u-shaped and extend outwards, and the extending parts can be placed on the bottleneck in an overlapping manner. According to the utility model, as common articles in daily life are selected, material can be easily obtained, and the manufacture is easy; the distance between an ultrasonic probe and the bottom of a culture dish can be adjusted as per experiment requirements; the device is simple and convenient to operate, and facilitates general popularization of the advanced technology.

Description

The transfected by ultrasound mediated destruction of microbubbles supplementary unit

Technical field

The utility model has related to a kind of medical experimental equipment, special design a kind of ultrasound microbubble mediated gene transfection supplementary unit.

Background technology

In recent years, along with the appearance of the targeted contrast agent with orthoselection effect, edify people and set up a kind of novel therapeutic genes target transmission system by means of the directional transmissions of ultrasonic non-invasive and collaborative microvesicle.Research shows, carry as carrier the activity that gene can keep these therapeutic substances with microbubble contrast agent, avoid them to be eliminated from circulation of blood, especially when gene delivery, because the liver that endogenous endonuclease is relevant with electric charge is known system, make naked DNA and other carriers at endovascular poor stability, very soon by metabolism; And under ul-trasonic irradiation, " cavitation effect " that ultrasonic microbubble produces acts on cytolemma, produce " outflow hole ", permeability of cell membrane increased, thus make the gene that discharges after microbubble destruction can the intravasation wall even tissue space promote the transfection of gene cross-film.But equipment complexity used when ultrasound microbubble mediated gene transfection is carried out in current most of laboratory, concrete operation method is also comparatively loaded down with trivial details.This just causes modern technique generally to promote.

Summary of the invention

The utility model, for existing the problems referred to above, has proposed a kind of ultrasound microbubble mediated gene transfection supplementary unit, and this supplementary unit equipment is simple, easy to operate.

The utility model is realized by following scheme: a kind of transfected by ultrasound mediated destruction of microbubbles supplementary unit, comprise uncovered large bottle, top at uncovered large bottle has a plurality of scale grooves that are arranged above and below, a pair of adjusting iron wire, a pair of carrying iron wire and be placed on the culture dish of carrying on iron wire, described carrying iron wire is fixed in large bottle, and described adjusting iron wire is positioned under the carrying iron wire and takes and is placed on the scale groove.

It is further: described scale groove interval 0.5cm.

Described a pair of adjusting iron wire is identical with a pair of carrying iron wire shape, is the u shape and extends an end to both sides, and extension can be taken and is placed on bottleneck.

Using method of the present utility model is as follows: will make full ultrasonic coupling agent in supplementary unit by oneself, and make the couplant level slightly surpass the culture dish bottom, ultrasound probe covers one deck preservative film, is fixed on and regulates on iron wire, is positioned at 1-2cm place, culture dish below.Adjust the distance of ultrasound probe and culture dish according to the experiment demand.

Of the present utility model having the following advantages: draw materials conveniently, select article common in daily life, make easily.Ultrasound probe and culture dish bottom spacing be easy to according to the experiment need to be adjusted.Device is simple, easy to operate.

The accompanying drawing explanation

Fig. 1 is the front view of the large bottle of the utility model.

Fig. 2 is sectional view of the present utility model;

In figure: 1, large bottle; 2, ultrasound probe; 3, regulate iron wire; 4, culture dish; 5, carrying iron wire; 6, scale groove.

Embodiment

Be below the utility model specific embodiment, and by reference to the accompanying drawings the technical solution of the utility model be further described, but the utility model is not limited to these embodiment.

As depicted in figs. 1 and 2, this transfected by ultrasound mediated destruction of microbubbles supplementary unit comprises that upper end has uncovered large bottle 1, a pair of adjusting iron wire 3, a pair of carrying iron wire 5 of scale groove 6 and is placed on the culture dish 4 on carrying iron wire 5, described carrying iron wire 5 is taken and is placed in large bottle 1, and described adjusting iron wire 3 is positioned at carrying iron wire 5 times and takes and is placed on scale groove 6.

Described scale groove 6 interval 0.5cm.The scale interval of this scale groove facilitates the distance between accurate adjustment ultrasound probe and culture dish bottom.

Described a pair of adjusting iron wire 3 is identical with a pair of carrying iron wire 5 shapes, is the u shape and extends an end to both sides, and extension can be taken and is placed on bottleneck.As shown in FIG., on large bottle, can symmetry establish 4 each grooves, be easy to stable carrying iron wire 5.

Concrete using method: will make full ultrasonic coupling agent in supplementary unit by oneself, and make the couplant level surpass the culture dish bottom.Cover one deck preservative film at ultrasound probe, with the soft iron silk, be fixed in and regulate on iron wire, make to be positioned at 1-2cm place, culture dish below.According to the experiment demand, regulate iron wire by handle and be placed on different scale grooves, can adjust the distance of ultrasound probe and culture dish.

Claims

1. a transfected by ultrasound mediated destruction of microbubbles supplementary unit, it is characterized in that: comprise uncovered large bottle (1), top at uncovered large bottle (1) has a plurality of scale grooves (6) that are arranged above and below, a pair of adjusting iron wire (3), a pair of carrying iron wire (5) and be placed on the culture dish (4) of (5) on the carrying iron wire, described carrying iron wire (5) is taken and is placed in large bottle (1), and described adjusting iron wire (3) is positioned at carrying iron wire (5) and descends and take to be placed on scale groove (6).

2. transfected by ultrasound mediated destruction of microbubbles supplementary unit according to claim 1, is characterized in that: the spacing 0.5cm between described scale groove (6).

3. transfected by ultrasound mediated destruction of microbubbles supplementary unit according to claim 1, it is characterized in that: described a pair of adjusting iron wire (3) is identical with a pair of carrying iron wire (5) shape, be the u shape and extend to both sides, extension can be taken on the bottleneck that is placed on uncovered large bottle (1).