CN203275283U - Portable ELISA (Enzyme Linked Immunosorbent Assay) meter - Google Patents
Portable ELISA (Enzyme Linked Immunosorbent Assay) meter Download PDFInfo
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- CN203275283U CN203275283U CN 201320327043 CN201320327043U CN203275283U CN 203275283 U CN203275283 U CN 203275283U CN 201320327043 CN201320327043 CN 201320327043 CN 201320327043 U CN201320327043 U CN 201320327043U CN 203275283 U CN203275283 U CN 203275283U
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- photoelectric
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Abstract
The utility model relates to a portable ELISA (Enzyme Linked Immunosorbent Assay) meter which comprises a photoelectric detection system and a signal processing system. The meter is characterized in that the photoelectric detection system comprises a photoelectric conversion circuit and a light emitting piece and a photoelectric battery which are connected with the photoelectric conversion circuit, wherein the light emitting piece and the photoelectric battery are located in a same axis in the vertical direction, a light shield plate with a light permeable hole is formed between the light emitting piece and the photoelectric battery, and a detection space for accommodating an object to be detected is arranged between the light shield plate and the photoelectric battery. The meter has the beneficial effects that the meter adopts a vertical light path structure to avoid problems such as measuring errors caused by inaccurate position of the object to be detected in a fast detection process in a horizontal light path structure, so that the meter is higher in detection precision. In addition, a system on chip singlechip microcomputer is adopted to realize calibration in site, analysis and processing of data, storage and communication of large volume data and the like, so that the work efficiency is higher.
Description
Technical field
The utility model relates to testing tool, particularly a kind of portable microplate reader of the on-the-spot quick test for food security and public health inspection and quarantine.
Background technology
Microplate reader is enzyme-linked immunosorbent assay instrument (ELISA Reader), is the instrumentation of enzyme linked immunosorbent assay.Enzyme-linked immunologic adsorption test method is called for short enzyme linked immunosorbent assay, is a kind of in labelling technique, is the modern technologies of a kind of sensitivity of developing from fluorescent antibody technics, isotope immunological technique, special, quick and energy robotization.The ultimate principle of enzyme linked immunosorbent assay is that antigen or antibody and enzyme are combined into enzyme-labelled antigen or antibody with glue crosslinking agent, this enzyme-labelled antigen or antibody can with solid phase carrier on or corresponding antigens or antibody generation idiosyncrasy in tissue, and still keep active immune complex in conjunction with forming securely.When adding corresponding substrate, substrate is presented the respective reaction color by enzymatic.Shade is directly proportional to corresponding antigens or antibody content.Because this technology is to be based upon on the basis of efficient catalytic effect of antigen-antibody reaction and enzyme, therefore, have sensitivity and the specificity of height, be a kind of immunological testing technology that is rich in vitality.The basic functional principle of microplate reader is Lambert-Beer's law, and its primary structure and photolometer are basic identical, and in fact it be exactly covert special photoelectric tintmeter or a spectrophotometer, namely analyzes antigen or Antibody concentration with colourimetry.ELISA measures the final volume of General Requirements test fluid below 250 μ l, can't complete test with general photolometer, thus to the photoelectric colorimetry in microplate reader in respect of specific (special) requirements.Although more existing patents, as " palm type ELIASA " (CN201035009), " enzyme mark instrument optical system " (CN201281708) etc., but each is all to adopt the horizontal optical path structure, this horizontal optical path structure, the additional measuring error that the position deviation that produces due to moving of detected material causes increases, and in addition, is inconvenient to take to on-the-spot the use, complicated operation, measuring accuracy is low.
Summary of the invention
Fundamental purpose of the present utility model is, for above-mentioned deficiency of the prior art, provides a kind of easy to carry, the portable microplate reader that measuring accuracy is high.
The technical scheme that the prior art problem that solves the utility model adopts is: a kind of portable microplate reader, it comprises photodetector system and signal processing system, its improvement is: described photodetector system comprises photoelectric switching circuit and the illuminating part and the photoelectric cell that are connected with described photoelectric switching circuit, described illuminating part and described photoelectric cell are positioned on the same axis of vertical direction, be provided with the shadow shield with a light hole between described illuminating part and described photoelectric cell, have the detection space that can hold detected material between described shadow shield and described photoelectric cell.
The below is further elaborated technique scheme:
Further, also comprise a servo drive system that moves in order to drive described photodetector system along continuous straight runs.
Further, described illuminating part is positioned at described photronic below.
Further, described signal processing system comprises
Signal pre-processing circuit, the output terminal of its input end and described photoelectric switching circuit is electrically connected, and converts voltage signal in order to the current signal with described photoelectric switching circuit output;
Central processing unit, its signal output port is connected with the output terminal of described signal pre-processing circuit, in order to calculate the penetrating light intensity of described detected material according to described voltage signal;
Memory circuit is electrically connected with described central processing unit;
Display circuit is electrically connected with described central processing unit.
Further, described signal processing system also comprises one in order to realize the data communication module of telecommunication, and described data communication module and described central processing unit are electrically connected.
Further, described signal processing system also comprises one in order to realize the usb circuit of data conversion storage, and described usb circuit and described central processing unit are electrically connected.
Further, described central processing unit is system on chip.
the beneficial effects of the utility model are: one, the utility model is by adopting vertical light channel structure, be that illuminating part and photoelectric cell are positioned on the same axis of vertical direction, the light that illuminating part sends forms light beam via the light hole on shadow shield, this light beam passes detected material and is radiated on photoelectric cell, in the fast detecting process, the location to occur unpunctual when detected material, as long as deviation distance is no more than himself diameter, light beam is worn to top from the bottom of detected material equally, so, can avoid in the horizontal optical path structure, owing to locating the inaccurate problems such as measuring error that cause because of detected material in the fast detecting process, its accuracy of detection is higher, two, adopting servo drive system to drive photodetector system in the utility model moves, with respect to for mobile detected material in detection, make structure compacter on the one hand, can make on the other hand photodetector system and the detected material error in moving less, guarantee the accuracy of location, so, further improve accuracy of detection and stability, three, adopt system on chip to realize analyzing and processing, mass data storage and communication of situ calibration, data etc., its work efficiency is higher.
Description of drawings
Fig. 1 is system architecture block scheme of the present utility model;
Fig. 2 is photodetector system structural representation of the present utility model;
Fig. 3 is signal pre-processing circuit figure of the present utility model;
Fig. 4 is key circuit wiring diagram of the present utility model;
Fig. 5 is display circuit key wiring diagram of the present utility model;
Fig. 6 is the key wiring diagram of data communication module of the present utility model.
Fig. 7 is usb circuit key wiring diagram of the present utility model.
Fig. 8 is complete circuit theory wiring diagram of the present utility model.
The realization of the utility model purpose, functional characteristics and advantage are described further with reference to accompanying drawing in connection with embodiment.
Embodiment
Describe the technical solution of the utility model in detail below with reference to drawings and the specific embodiments, so as clearer, understand invention essence of the present utility model intuitively.
with reference to Figure 1 and Figure 2, the utility model provides a kind of portable microplate reader, it comprises photodetector system 10 and signal processing system 20, described photodetector system 10 comprises photoelectric switching circuit and the illuminating part 102 and the photoelectric cell 101 that are connected with described photoelectric switching circuit, described illuminating part 102 is positioned on the same axis of vertical direction with described photoelectric cell 101, it is the vertical optical path structure, better, described illuminating part 102 is positioned at the below of described photoelectric cell 101, be provided with the shadow shield 30 with a light hole 301 between described illuminating part 102 and described photoelectric cell 101, has the detection space that can hold detected material 40 between described shadow shield 30 and described photoelectric cell 101, this detection space is used for placing detected material 40, for example, by being placed on after the container splendid attire between illuminating part 102 and photoelectric cell 101, the general light emitting diode that adopts of illuminating part 102, photoelectric cell 101 can adopt selenium cell 101 etc., the light that light emitting diode sends forms light beam by the light hole 301 on shadow shield 30, light beam passes detected material 40 and exposes on photoelectric cell 101, photoelectric cell 101 produces electromotive force, and in the opto-electronic conversion electric current generation current signal, this current signal is transferred to signal processing system 20.
owing to adopting above-mentioned vertical light channel structure, be that illuminating part 102 is positioned on the same axis of vertical direction with photoelectric cell 101, the light that illuminating part 102 sends forms light beam via the light hole 301 on shadow shield 30, this light beam passes detected material 40 and is radiated on photoelectric cell 101, in the fast detecting process, the location to occur unpunctual when detected material 40, as long as deviation distance is no more than himself diameter, light beam is worn to top from the bottom of detected material 40 equally, so, can avoid in the horizontal optical path structure, owing to locating the inaccurate problems such as measuring error that cause because of detected material 40 in the fast detecting process, its accuracy of detection is higher.
in addition, in the utility model, also comprise a servo drive system that moves in order to drive described photodetector system 10 along continuous straight runs, in concrete the detection, driving photodetector system 10 by servo drive system moves horizontally, to realize that a plurality of detected materials 40 are carried out fast detecting, this detection mode and structure, with respect to for mobile detected material 40 in detection, make structure compacter on the one hand, can make on the other hand photodetector system 10 and detected material 40 error in moving less, guarantee the accuracy of location, so, further improve accuracy of detection and stability.
Or with reference to shown in 1, signal processing system 20 comprises signal pre-processing circuit 201, central processing unit 202, memory circuit 203, display circuit 204 and key circuit 207, the output terminal of signal pre-processing circuit 201 input ends and described photoelectric switching circuit is electrically connected, and converts voltage signal in the voltage range that central processing unit 202 is convenient to process and precision is high in order to the current signal with described photoelectric switching circuit output; The signal output port of central processing unit 202 is connected with the output terminal of described signal pre-processing circuit 201, in order to calculating the penetrating light intensity of described detected material 40 according to described voltage signal, analyze antigen and Antibody concentration in its detected material 40 according to penetrating light intensity; Memory circuit 203, display circuit 204 and case circuit are electrically connected with described central processing unit 202 respectively; In addition, this signal processing system 20 also comprises one in order to the data communication module 205 that realizes telecommunication and the usb circuit 206 in order to realize data conversion storage, and data communication module 205 and usb circuit 206 are electrically connected with described central processing unit 202 respectively.
with reference to shown in Figure 3, the effect of signal pre-processing circuit 201 is to make the current signal of photoelectric switching circuit convert the voltage range that single-chip microcomputer is convenient to process and precision is high to be input to central processing unit 202, central processing unit 202 adopts SOC (system on a chip) (SOC) single-chip microcomputer C8051F020, concrete, current signal by photoelectric switching circuit induction output converts voltage signal to through sample resistance R8, suppress external interference by the single order passive low ventilating filter that is consisted of by R7 and C7 again, by R9, the in-phase proportion amplifier that R10 and operational amplifier consist of is amplified to suitable voltage range, pass through R11, R12 consists of bleeder circuit and described voltage signal is exported to the data acquisition interface of single-chip microcomputer, the effect of bleeder circuit is the input resistance of coupling single-chip microcomputer, measuring accuracy is guaranteed.
By the digital signal that the single-chip data acquisition interface obtains, according to lambert-beer's law, utilize formula:
A=OD=logΙ0/Ι （1）
Calculate and obtain absorbance (transmittance).In formula (1): Ι 0 is the light intensity before detecting thing, and Ι is the light intensity from detected material 40 outgoing.In the present embodiment, Ι 0 obtains (being that output display is 0 value) by the detection to standard model, and Ι is the penetrating light intensity that detected test product (detected material 40) is obtained.
Calculate the transmittance of the detected test product that obtains according to formula (1), when adopting the vertical optical path structure, because the surface tension of the detected test product of variable concentrations is different, the data output of calculating gained OD value and antigen or antibody content (concentration) is nonlinear relationship, can adopt non-linear correction method to realize linearization.
To shown in Figure 5, Fig. 4 is the keyboard setting principle figure of microplate reader with reference to Fig. 4, and the Low level effective mode is identified keyboard operation.Fig. 5 is the schematic diagram of display circuit 204, what the display screen of display circuit 204 was selected is the C0802B module, according to the requirement of each signal pins of C0802B module to level and sequential, adopt the mode of bus-sharing to connect, 74HC573 is address latch, and the effect of 74HLS00 is to satisfy display screen to the requirement of sequential, No. four pin wherein is connected in the Y2 pin of 74HC138 code translator, kept MCU plug-in other, as data communication port, printout mouth etc., the ability of peripheral hardware.
Extremely shown in Figure 8 with reference to Fig. 6, radio communication and USB unloading output dual mode that the utility model provides data communication function to comprise data, corresponding circuit module comprises data communication module 205 and US interface circuit, wherein, data communication module as shown in Figure 6, select the nRF2401 radio transmitting and receiving chip of Nordic company to complete data communication, nRF2401 has two kinds of transceiver mode: Shock Burst pattern and Duo Ceiver pattern.Adopt the transmission of the Shock Burst Pattern completion electrocardiogram (ECG) data of nRF240l in the design.The nRF240l radio transmitting and receiving chip adopts the SPI line to be connected with C805 lF020 single-chip microcomputer hardware interface.In Fig. 8, the P2.1 of C8051F020 single-chip microcomputer, P2.2 and P2.3 pin are configured to general I/O pin, are connected with the CS pin with PWR_UP, the CE of nRF240l radio transmitting and receiving chip, control the working method of nRF2401 radio transmitting and receiving chip; The SPI(P2.4 of C8051F020 single-chip microcomputer) DRl, the CLKl of pin and nRF2401 radio transmitting and receiving chip are connected with DATA, consist of the SPI serial communication.During system initialization, at first the C805lF020 single-chip microcomputer puts the nRF2401 radio transmitting and receiving chip is configuration mode, nRF2401 radio transmitting and receiving chip status word is configured, and enters state of activation.When needs send new data, it is high level that the C8051F020 single-chip microcomputer is put CE, the receiver address of this moment and payload data are as the internal clocking of nRF2401 radio transmitting and receiving chip, (also available MCU) transfers to lMbps with speed with request protocol, and putting CE is that low level activates Shock Burst emission; Receive data adopts CE, DRl, CLKl and DATA to realize controlling: behind address and size that radio frequency bag input load correctly is set, putting CE is that high level activates reception, the nRF2401 radio transmitting and receiving chip detects input information 200 μ s, receiving effectively the nRF2401 radio transmitting and receiving chip, to put DRl be high level, after system received new data, putting DRl was low level.Usb circuit 206 selects usb bus common apparatus interface chip CH372 as the data exchange processing chip as shown in Figure 9, utilizes the underlying protocol in the built-in USB communication of CH372, makes Single Chip Microcomputer (SCM) program very succinct.At this moment, portable microplate reader is equivalent to a USB unit of plug-in PC, thereby realizes exchanges data.The effect of chip SP0503BAJT is for suppressing ESD and other transient overvoltages, to satisfy the requirement of electromagnetic compatibility.
In sum, the utility model can be avoided in the horizontal optical path structure by adopting vertical light channel structure, and owing to locating the inaccurate problems such as measuring error that cause because of detected material 40 in the fast detecting process, its accuracy of detection is higher; Simultaneously, adopting servo drive system to drive photodetector system 10 moves, with respect to for mobile detected material 40 in detection, make structure compacter on the one hand, can make on the other hand photodetector system 10 and detected material 40 error in moving less, guarantee the accuracy of location, so, further improve accuracy of detection and stability; In addition, adopt system on chip to realize analyzing and processing, mass data storage and communication of situ calibration, data etc., its work efficiency is higher.
The above is only preferred embodiment of the present utility model; not thereby limit its scope of the claims; every equivalent structure or equivalent flow process conversion that utilizes the utility model instructions and accompanying drawing content to do; directly or indirectly be used in other relevant technical fields, all in like manner be included in scope of patent protection of the present utility model.
Claims (7)
1. portable microplate reader, it comprises photodetector system and signal processing system, it is characterized in that: described photodetector system comprises photoelectric switching circuit and the illuminating part and the photoelectric cell that are connected with described photoelectric switching circuit, described illuminating part and described photoelectric cell are positioned on the same axis of vertical direction, be provided with the shadow shield with a light hole between described illuminating part and described photoelectric cell, have the detection space that can hold detected material between described shadow shield and described photoelectric cell.
2. portable microplate reader according to claim 1, is characterized in that: also comprise a servo drive system that moves in order to drive described photodetector system along continuous straight runs.
3. portable microplate reader according to claim 1, it is characterized in that: described illuminating part is positioned at described photronic below.
4. portable microplate reader according to claim 1, it is characterized in that: described signal processing system comprises
Signal pre-processing circuit, the output terminal of its input end and described photoelectric switching circuit is electrically connected, and converts voltage signal in order to the current signal with described photoelectric switching circuit output;
Central processing unit, its signal output port is connected with the output terminal of described signal pre-processing circuit, in order to calculate the penetrating light intensity of described detected material according to described voltage signal;
Memory circuit is electrically connected with described central processing unit;
Display circuit is electrically connected with described central processing unit.
5. portable microplate reader according to claim 4 is characterized in that: described signal processing system also comprises one in order to realize the data communication module of telecommunication, and described data communication module and described central processing unit are electrically connected.
6. portable microplate reader according to claim 4 is characterized in that: described signal processing system also comprises one in order to realize the usb circuit of data conversion storage, and described usb circuit and described central processing unit are electrically connected.
7. portable microplate reader according to claim 4, it is characterized in that: described central processing unit is system on chip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320327043 CN203275283U (en) | 2013-06-07 | 2013-06-07 | Portable ELISA (Enzyme Linked Immunosorbent Assay) meter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320327043 CN203275283U (en) | 2013-06-07 | 2013-06-07 | Portable ELISA (Enzyme Linked Immunosorbent Assay) meter |
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| Publication Number | Publication Date |
|---|---|
| CN203275283U true CN203275283U (en) | 2013-11-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201320327043 Expired - Fee Related CN203275283U (en) | 2013-06-07 | 2013-06-07 | Portable ELISA (Enzyme Linked Immunosorbent Assay) meter |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113588630A (en) * | 2021-07-13 | 2021-11-02 | 曲靖师范学院 | Colorimetric method detector and detection method thereof |
| CN114965991A (en) * | 2022-04-02 | 2022-08-30 | 重庆默赛乐生物科技有限公司 | Portable ELIASA with preliminary treatment structure |
-
2013
- 2013-06-07 CN CN 201320327043 patent/CN203275283U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113588630A (en) * | 2021-07-13 | 2021-11-02 | 曲靖师范学院 | Colorimetric method detector and detection method thereof |
| CN114965991A (en) * | 2022-04-02 | 2022-08-30 | 重庆默赛乐生物科技有限公司 | Portable ELIASA with preliminary treatment structure |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131106 Termination date: 20160607 |