CN203229542U - Gas-liquid pressure loading cell culture device - Google Patents
Gas-liquid pressure loading cell culture device Download PDFInfo
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- CN203229542U CN203229542U CN 201320282620 CN201320282620U CN203229542U CN 203229542 U CN203229542 U CN 203229542U CN 201320282620 CN201320282620 CN 201320282620 CN 201320282620 U CN201320282620 U CN 201320282620U CN 203229542 U CN203229542 U CN 203229542U
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- liquid pressure
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Abstract
The utility model discloses a gas-liquid pressure loading cell culture device, aiming at solving the problems of slow amplification speed, complicated structure, and difficulty in operation of the existing culture device. The gas-liquid pressure loading cell culture device comprises a cell culture groove, a culture medium inlet arranged at the lower part of one side of the cell culture groove, a culture medium outlet arranged at the lower part of the other side of the cell culture groove, and a sealing cover arranged at the top of the cell culture groove and used for sealing the cell culture groove, wherein the sealing cover is provided with an air inlet and an air outlet. The gas-liquid pressure loading cell culture device has a simple structure and is convenient to operate, air is aerated into the cell culture groove from the top so as to press the culture medium, the exact pressure is obtained through a pressure inductor, the load is easy to transfer, the cells are uniformly pressed, and the real condition in a body can be better simulated, especially for research on regeneration of a liver with part of the liver cut off, and the gas-liquid pressure loading cell culture device can be used for well simulating the impact of the change of shearing stress on liver sinusoidal endothelial cells after hepatectomy.
Description
Technical field
The utility model relates to a kind of gas-liquid pressure and loads cell culture apparatus.
Background technology
At present by developmental biology is discovered, stem cell and somatocyte play an important role in tissue formation and regenerative process, but content is relatively low in vivo for a lot of cells, the difficulty of drawing materials, and generally be slow cycle cell, even have unlimited proliferation potential as stem cell, but be difficult to the demand that rapid amplifying reaches organizational project.Therefore, how in a large number the rapid amplifying cell becomes the focus of research.Conventional device exists that amplification rate is slow, structure is complicated, the problem of operational difficulty.
The utility model content
The purpose of this utility model is to provide a kind of gas-liquid pressure to load cell culture apparatus, and it is slower to solve existing culture apparatus amplification rate, and structure is complicated, the problem of operational difficulty.
To achieve these goals, the technical solution adopted in the utility model is as follows:
Gas-liquid pressure loads cell culture apparatus, comprise the cell cultures groove, be arranged at the substratum import of cell cultures groove one side lower part, be arranged at the substratum outlet of cell cultures groove opposite side bottom, and be arranged at cell cultures groove top for the sealing cover with the sealing of cell cultures groove; Described sealing cover is provided with gas feed and pneumatic outlet.
Further, described cell cultures groove inner bottom part central authorities also are provided with for the silica gel elastic force film of placing cell.
Again further, described cell cultures groove inner bottom part also is provided with pressure inductor near substratum ingress.
A nearlyer step ground, described cell cultures groove is cube structure, described substratum import and substratum outlet are positioned on the same sea line.
The utility model compared with prior art has the following advantages and beneficial effect:
(1) the utility model is simple in structure, easy to operate, feed gas from cell cultures groove top substratum is exerted pressure, understand concrete pressure accurately by pressure inductor simultaneously, be easy to transmitted load and the cell stand under load is even, the time of day in the analogue body that can be preferable; Especially to the research of liver regeneration behind the partially hepatectomized, the variation of shearing stress is to the influence of sinusoidal endothelial cell after the simulation hepatectomy that can be preferable.
(2) the utility model required substratum in the culturing cell process is less, and can detect relevant cell factor accurately.
(3) the utility model is provided with the outlet of substratum import and substratum, can change the substratum of cell cultures groove very easily, for the growth of cell provides enough nutrients.
Description of drawings
Fig. 1 is structural representation of the present utility model.
Wherein, the component name of mark correspondence is called in the accompanying drawing: 1-cell cultures groove, the import of 2-substratum, the outlet of 3-substratum, 4-sealing cover, 5-gas feed, 6-pneumatic outlet, 7-silica gel elastic force film, 8-pressure inductor.
Embodiment
The utility model is described in further detail below in conjunction with drawings and Examples, and embodiment of the present utility model includes but not limited to the following example.
Embodiment
As shown in Figure 1, gas-liquid pressure loads cell culture apparatus, comprise that cube structure and upper end are open cell cultures groove 1, be arranged at cell cultures groove 1 one side lower parts for the substratum import 2 that feeds substratum, be arranged at cell cultures groove 1 opposite side bottom and be used for discharging the substratum outlet 3 of substratum, and be arranged at cell cultures groove 1 top for the sealing cover 4 with 1 sealing of cell cultures groove; Described sealing cover 4 is provided with gas feed 5 and pneumatic outlet 6.Setting can be changed substratum in the cell cultures groove smoothly by the outlet of substratum import and substratum like this, for the growth of cell provides enough nutrients; Simultaneously, feed corresponding gas by gas feed substratum is exerted pressure, thus the time of day accurately in the analogue body, to improve cell amplification speed.In order better to realize at pneumatic outlet and gas ingress a corresponding valve being set all, to control the pressure in the cell cultures groove accurately by the utility model.
For suitable study condition is provided to cell, described cell cultures groove 1 inner bottom part central authorities also are provided with for the silica gel elastic force film 7 of placing cell.
Simultaneously, in order to understand the concrete pressure in the cell cultures groove accurately, reach appropriate value to guarantee pressure, described cell cultures groove 1 inner bottom part also is provided with pressure inductor 8 near substratum import 2 places.When pressure hour, only need close the valve of gas outlet, feed gas to increase the pressure in the cell cultures groove from gas feed; When pressure is excessive, only need close the valve of gas feed, discharge certain gas to reduce the pressure in the cell cultures groove from pneumatic outlet; Wherein, the particular case of cell cultures groove internal pressure can directly be learnt by pressure inductor.
In order better to realize the utility model, described substratum import 2 is positioned on the same sea line with substratum outlet 3, thereby has guaranteed to change smoothly the substratum of cell cultures groove.
According to above-described embodiment, just can realize the utility model well.What deserves to be explained is; under the prerequisite based on the said structure design; for solving same technical problem; some do not have substantial change or polishing even in that the utility model is made; the essence of the technical scheme that adopts is still the same with the utility model, so it also should be in protection domain of the present utility model.
Claims (4)
1. gas-liquid pressure loads cell culture apparatus, it is characterized in that, comprise cell cultures groove (1), be arranged at the substratum import (2) of cell cultures groove (1) one side lower part, be arranged at the substratum outlet (3) of cell cultures groove (1) opposite side bottom, and be arranged at cell cultures groove (1) top for the sealing cover (4) with cell cultures groove (1) sealing; Described sealing cover (4) is provided with gas feed (5) and pneumatic outlet (6).
2. gas-liquid pressure according to claim 1 loads cell culture apparatus, it is characterized in that, described cell cultures groove (1) inner bottom part central authorities also are provided with for the silica gel elastic force film (7) of placing cell.
3. gas-liquid pressure according to claim 2 loads cell culture apparatus, it is characterized in that, described cell cultures groove (1) inner bottom part locates also to be provided with pressure inductor (8) near substratum import (2).
4. gas-liquid pressure according to claim 3 loads cell culture apparatus, it is characterized in that described cell cultures groove (1) is cube structure, and described substratum import (2) is positioned on the same sea line with substratum outlet (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320282620 CN203229542U (en) | 2013-05-22 | 2013-05-22 | Gas-liquid pressure loading cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320282620 CN203229542U (en) | 2013-05-22 | 2013-05-22 | Gas-liquid pressure loading cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203229542U true CN203229542U (en) | 2013-10-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320282620 Expired - Fee Related CN203229542U (en) | 2013-05-22 | 2013-05-22 | Gas-liquid pressure loading cell culture device |
Country Status (1)
| Country | Link |
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| CN (1) | CN203229542U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105586249A (en) * | 2016-03-07 | 2016-05-18 | 浙江大学 | Circulating perfusion biological reactor device capable of achieving circulating perfusion of three-dimensional support |
| CN105974102A (en) * | 2016-04-28 | 2016-09-28 | 新乡医学院 | Apparatus for observing shear stress adjusted PCSK9 expression |
-
2013
- 2013-05-22 CN CN 201320282620 patent/CN203229542U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105586249A (en) * | 2016-03-07 | 2016-05-18 | 浙江大学 | Circulating perfusion biological reactor device capable of achieving circulating perfusion of three-dimensional support |
| CN105586249B (en) * | 2016-03-07 | 2021-04-06 | 浙江大学 | Circulating perfusion bioreactor device capable of realizing circulating perfusion of three-dimensional support |
| CN105974102A (en) * | 2016-04-28 | 2016-09-28 | 新乡医学院 | Apparatus for observing shear stress adjusted PCSK9 expression |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131009 Termination date: 20160522 |