CN203123622U - Column chromatography continuous elution device used for macro-porous resin enriched type effective part - Google Patents
Column chromatography continuous elution device used for macro-porous resin enriched type effective part Download PDFInfo
- Publication number
- CN203123622U CN203123622U CN 201320036627 CN201320036627U CN203123622U CN 203123622 U CN203123622 U CN 203123622U CN 201320036627 CN201320036627 CN 201320036627 CN 201320036627 U CN201320036627 U CN 201320036627U CN 203123622 U CN203123622 U CN 203123622U
- Authority
- CN
- China
- Prior art keywords
- chromatographic column
- column chromatography
- active component
- stainless steel
- device used
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The utility model discloses a column chromatography continuous elution device used for a macro-porous resin enriched type effective part. The column chromatography continuous elution device used for the macro-porous resin enriched type effective part comprises a chromatographic column and a stainless steel rack, wherein the chromatographic column is arranged on the stainless steel rack in a connected manner through a fixing piece; the roller is characterized in that a liquid storage device used in cooperation with an opening in the upper end of the chromatographic column is arranged on the stainless steel rack; and an eluant collecting device used in cooperation with a liquid outlet of the chromatographic column is arranged below the chromatographic column. According to the column chromatography continuous elution device used for the macro-porous resin enriched type effective part provided by the utility model, the liquid storage device and the eluant collecting device are respectively arranged above and below the chromatographic column, so that continuous elution can be realized; a spiral clip is arranged on a rubber pipe, so that the flow velocity of eluant can be effectively controlled, and the velocity of eluant flowing into the chromatographic column is equivalent to the velocity of the eluant flowing out of the chromatographic column; and after the flow velocity is adjusted, the column chromatography continuous elution device can be used for continuously eluting for long time in an unmanned condition, even eluting overnight, so that not only can the labor be saved but also the working efficiency is improved.
Description
Technical field
The utility model relates to a kind of wash-out device, is specifically related to the continuous wash-out device of a kind of column chromatography for the macroporous resin enrichment active component.
Background technology
Macroporous absorbent resin has been widely used in enrichment and the purifying of active ingredient in Chinese medicine and the natural drug extract, at present, be used for the column chromatography of macroporous resin enrichment active component, applied sample amount is bigger usually, the time of gradient elution is longer, in the process of gradient elution, because the volume of pillar is less, operating personnel need keep on the pillar next door, in order in time in pillar, add eluent, unless you give your whole attention to it, the phenomenon that eluent drains off in the pillar will occur, thereby have influence on separating effect; In addition, frequently add eluent, increased operating personnel's the amount of labour, go out or the period at night if operating personnel are busy, all need before leaving, pillar be turned off, stop elution process, lower operating efficiency greatly, had influence on normally carrying out of operation simultaneously again.
Summary of the invention
At problems of the prior art, the utility model purpose is providing a kind of column chromatography for the macroporous resin enrichment active component continuous wash-out device.
The utility model is realized by the following technical programs:
The continuous wash-out device of described a kind of column chromatography for the macroporous resin enrichment active component, comprise chromatographic column, stainless steel frame, chromatographic column is arranged on the stainless steel frame by the fixture connection, it is characterized in that described stainless steel frame is provided with the device for storing liquid that is used with the chromatographic column upper end open, the below of described chromatographic column is provided with the eluent gathering-device that is used with the chromatographic column fluid hole.
Described a kind of continuous wash-out device of column chromatography for punching resin concentration active component is characterized in that described fixture is extension clamp or iron ring.
The continuous wash-out device of described a kind of column chromatography for the macroporous resin enrichment active component, it is characterized in that described device for storing liquid is narrow-mouthed bottle, the lower end of described narrow-mouthed bottle sidewall is provided with liquid outlet, peg graft on the described liquid outlet and be provided with rubber stopper with holes, peg graft in the hole of described rubber stopper and be provided with the glass tube of hollow, one end of described glass tube extend in the narrow-mouthed bottle, and socket is provided with first rubber tube on the other end, and an other end of first rubber tube extend in the chromatographic column.
The continuous wash-out device of described a kind of column chromatography for the macroporous resin enrichment active component is characterized in that described first rubber tube is provided with first screw clamp.
The continuous wash-out device of described a kind of column chromatography for the macroporous resin enrichment active component, it is characterized in that described eluent gathering-device receives bucket for collecting liquid, socket is provided with second rubber tube on the described chromatographic column fluid hole, and an other end of described second rubber tube extend into to be collected in the liquid reception bucket.
The continuous wash-out device of described a kind of column chromatography for the macroporous resin enrichment active component is characterized in that described second rubber tube is provided with second screw clamp.
The utility model arranges device for storing liquid and eluent gathering-device respectively by the above and below in chromatographic column, realize continuous wash-out, by at rubber tube screw clamp being set, can effectively control the flow velocity of eluent, eluent is flowed into and to flow out the flow velocity of chromatographic column suitable, adjust flow velocity after, this device can be under unmanned situation the long-time continuous wash-out, even wash-out all through the night, namely save manpower and improved operating efficiency again.
Description of drawings
Fig. 1 is structural representation of the present utility model;
Among the figure: the 1-stainless steel frame, the 2-narrow-mouthed bottle, the 3-liquid outlet, the 4-rubber stopper, the 5-glass tube, 6-first rubber tube, 7-first screw clamp, the 8-fixture, the 9-chromatographic column, 10-collects liquid and receives bucket, 11-second screw clamp, 12-second tube connector.
The specific embodiment
Below in conjunction with Figure of description the utility model is described in further detail, and provides the specific embodiment.
The continuous wash-out device of a kind of column chromatography for the macroporous resin enrichment active component, comprise chromatographic column 9, stainless steel frame 1, chromatographic column 9 is arranged on the stainless steel frame 1 by 8 connections of fixtures such as extension clamp or iron ring, when adopting extension clamp fixedly, one end of extension clamp and stainless steel frame 1 are fixing, and the other end is clamped the middle part of chromatographic column 9; When adopting iron ring fixedly, the connection handle that will be arranged on the iron ring with clip is fixed on the stainless steel frame 1, and the iron ring socket is arranged on the middle part of chromatographic column 9, during practical operation, no matter be extension clamp or iron ring, the position that contacts with chromatographic column 9 needs with the cloth parcel, to avoid bumping to glass column 9; Stainless steel frame 1 is provided with the device for storing liquid that is used with chromatographic column 9 upper end open, this device for storing liquid cocoa adopts narrow-mouthed bottle 2, lower end at narrow-mouthed bottle 2 sidewalls is provided with liquid outlet 3, peg graft on the liquid outlet 3 and be provided with rubber stopper 4 with holes, peg graft in the hole and be provided with the glass tube 5 of hollow, one end of glass tube 5 extend in the narrow-mouthed bottle 2, socket is provided with first rubber tube 6 on the other end, an other end of first rubber tube 6 extend in the chromatographic column 9, at first rubber tube 6 first screw clamp 7 is set, controls flow velocity by the degree of tightness of regulating first screw clamp 7.Narrow-mouthed bottle 2 capacity can be 10～20 liters, are placed on the stainless steel shelf, because its capacity is big, eluent can be prepared in advance and pour into wherein, are used for continuous wash-out.
The below of chromatographic column 9 is provided with the eluent gathering-device that is used with the chromatographic column fluid hole, this eluent gathering-device can adopt collects liquid reception bucket 10, regulate the piston of chromatographic column 9 belows, the control flow velocity, if chromatographic column 9 receives bucket 10 with collection liquid certain distance is arranged, also can on the chromatographic column fluid hole, socket be provided with second rubber tube 12, an other end of second rubber tube 12 extend into to be collected in the liquid reception bucket 10, second rubber tube 12 is provided with second screw clamp 11, the degree of tightness of regulating piston and second screw clamp 11 is controlled flow velocity, make that the flow velocity of eluent inflow and outflow chromatographic column 9 is suitable, even if operating personnel leave like this, experiment also can be proceeded, and realizes continuous wash-out.
When operation is used, according to shown in Figure 1 each parts is assembled, in chromatographic column 9, install macroporous absorbent resin filler and sample size, use the eluent wash-out, pour in the narrow-mouthed bottle eluent for preparing in advance into 2, open first screw clamp 7, piston and second screw clamp 11 and adjust flow velocity, both flow velocitys are quite got final product.This wash-out device is simple to operate, and is easy to use, and the liquid storage amount is big, particularly after up and down flow velocity regulates, can be under unmanned situation the long-time continuous wash-out, manpower is namely saved in even operation all through the night, has improved operating efficiency again.
Claims (6)
1. continuous wash-out device of column chromatography that is used for the macroporous resin enrichment active component, comprise chromatographic column, stainless steel frame, chromatographic column is arranged on the stainless steel frame by the fixture connection, it is characterized in that described stainless steel frame is provided with the device for storing liquid that is used with the chromatographic column upper end open, the below of described chromatographic column is provided with the eluent gathering-device that is used with the chromatographic column fluid hole.
2. a kind of continuous wash-out device of column chromatography for punching resin concentration active component as claimed in claim 1 is characterized in that described fixture is extension clamp or iron ring.
3. the continuous wash-out device of a kind of column chromatography for the macroporous resin enrichment active component as claimed in claim 1, it is characterized in that described device for storing liquid is narrow-mouthed bottle, the lower end of described narrow-mouthed bottle sidewall is provided with liquid outlet, peg graft on the described liquid outlet and be provided with rubber stopper with holes, peg graft in the hole of described rubber stopper and be provided with the glass tube of hollow, one end of described glass tube extend in the narrow-mouthed bottle, socket is provided with first rubber tube on the other end, and an other end of first rubber tube extend in the chromatographic column.
4. the continuous wash-out device of a kind of column chromatography for the macroporous resin enrichment active component as claimed in claim 3 is characterized in that described first rubber tube is provided with first screw clamp.
5. the continuous wash-out device of a kind of column chromatography for the macroporous resin enrichment active component as claimed in claim 1, it is characterized in that described eluent gathering-device receives bucket for collecting liquid, socket is provided with second rubber tube on the described chromatographic column fluid hole, and an other end of described second rubber tube extend into to be collected in the liquid reception bucket.
6. the continuous wash-out device of a kind of column chromatography for the macroporous resin enrichment active component as claimed in claim 5 is characterized in that described second rubber tube is provided with second screw clamp.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320036627 CN203123622U (en) | 2013-01-21 | 2013-01-21 | Column chromatography continuous elution device used for macro-porous resin enriched type effective part |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320036627 CN203123622U (en) | 2013-01-21 | 2013-01-21 | Column chromatography continuous elution device used for macro-porous resin enriched type effective part |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203123622U true CN203123622U (en) | 2013-08-14 |
Family
ID=48931652
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320036627 Expired - Fee Related CN203123622U (en) | 2013-01-21 | 2013-01-21 | Column chromatography continuous elution device used for macro-porous resin enriched type effective part |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203123622U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104155169A (en) * | 2014-07-21 | 2014-11-19 | 中国科学院深圳先进技术研究院 | Separating device |
-
2013
- 2013-01-21 CN CN 201320036627 patent/CN203123622U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104155169A (en) * | 2014-07-21 | 2014-11-19 | 中国科学院深圳先进技术研究院 | Separating device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203954732U (en) | A kind of Multi-channel solid phase extraction device | |
| CN202237381U (en) | Novel, simple and easy solid phase extract device | |
| CN203123622U (en) | Column chromatography continuous elution device used for macro-porous resin enriched type effective part | |
| CN202822863U (en) | Embedded type solid phase extraction device | |
| CN208302256U (en) | A kind of height-adjustable solid-phase extracting instrument device of outflow liquid level | |
| CN203440269U (en) | Chromatography device for purified plasma proteins | |
| CN204261380U (en) | A kind of adsorption separation device of purifying chlorogenic acid | |
| CN203954733U (en) | A kind of fluid sample organic matter extracting and purifying device | |
| CN206095760U (en) | Binary channels aquatic trace organic pollutant enrichment device | |
| CN204815785U (en) | Automatic control chromatographic device | |
| CN212467209U (en) | Automatic change chromatography device | |
| CN201033310Y (en) | Magnetic separation holder suitable for many kinds of centrifuge tube | |
| CN203447822U (en) | Chromatographic column suitable for 96-well tissue culture plate | |
| CN205287717U (en) | Be used for column chromatography elution pressurization inlet means | |
| CN205392502U (en) | Filtration stand is used in laboratory | |
| CN201993352U (en) | Continuous sample injection chromatographic column device | |
| CN203400527U (en) | Efficient chromatography system | |
| CN204373978U (en) | A kind of rapid filtering device for laboratory fluids sample | |
| CN206045570U (en) | The integrated mobile phase defecator that a kind of HPLC chromatogram is used | |
| CN210736767U (en) | Nucleic acid quantitative analysis detector | |
| CN205412434U (en) | Pressure pole filter equipment | |
| CN205516627U (en) | Solid phase extractor | |
| CN209476013U (en) | It is a kind of for automating the device of impurity screening or moisture | |
| CN202822865U (en) | Water removal device for biological fermentation liquor | |
| CN203342438U (en) | Plant extracting chromatographic purification device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130814 Termination date: 20160121 |
|
| EXPY | Termination of patent right or utility model |