CN212467209U - Automatic change chromatography device - Google Patents
Automatic change chromatography device Download PDFInfo
- Publication number
- CN212467209U CN212467209U CN202020636525.5U CN202020636525U CN212467209U CN 212467209 U CN212467209 U CN 212467209U CN 202020636525 U CN202020636525 U CN 202020636525U CN 212467209 U CN212467209 U CN 212467209U
- Authority
- CN
- China
- Prior art keywords
- controller
- feeding
- chromatography
- tank
- chromatography column
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000004587 chromatography analysis Methods 0.000 title claims abstract description 44
- 239000007788 liquid Substances 0.000 claims abstract description 32
- 238000010828 elution Methods 0.000 claims abstract description 20
- 230000008929 regeneration Effects 0.000 claims abstract description 19
- 238000011069 regeneration method Methods 0.000 claims abstract description 19
- 238000005406 washing Methods 0.000 claims abstract description 19
- 239000012535 impurity Substances 0.000 claims abstract description 18
- 238000002347 injection Methods 0.000 claims abstract description 17
- 239000007924 injection Substances 0.000 claims abstract description 17
- 239000003480 eluent Substances 0.000 claims abstract description 7
- 238000000746 purification Methods 0.000 claims description 15
- 239000002351 wastewater Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000000741 silica gel Substances 0.000 claims description 4
- 229910002027 silica gel Inorganic materials 0.000 claims description 4
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 230000002572 peristaltic effect Effects 0.000 claims description 3
- 238000005070 sampling Methods 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 238000011002 quantification Methods 0.000 claims description 2
- 239000011347 resin Substances 0.000 abstract description 6
- 229920005989 resin Polymers 0.000 abstract description 6
- 238000005457 optimization Methods 0.000 abstract description 4
- 238000011161 development Methods 0.000 abstract description 3
- 230000018109 developmental process Effects 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 24
- 239000000463 material Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 229940088710 antibiotic agent Drugs 0.000 description 3
- 238000004440 column chromatography Methods 0.000 description 2
- 239000000945 filler Substances 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000012521 purified sample Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Images
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The utility model discloses an automatic change chromatography device has introduced automatic control system on traditional chromatography device basis. Sample loading liquid, impurity washing liquid, eluent and regeneration liquid are prepared in advance, and after sample injection and outflow parameters are set through an automatic control system, sample loading, impurity washing, elution and resin regeneration can be automatically completed under unattended condition. The chromatographic experiment can be conveniently carried out by utilizing off-duty time, and the chromatographic process optimization time can be greatly shortened and the project development progress can be shortened under the condition that a plurality of groups of chromatographic systems are matched for use.
Description
Technical Field
The utility model relates to a chromatographic device, concretely relates to laboratory is with automatic change chromatographic device who realizes mixture separation purification.
Background
In the field of pharmaceutical chemical industry, column chromatography is a common technical means for separation and purification, the existing chromatography process is divided into a plurality of steps such as sample loading, impurity washing, elution, resin regeneration and the like, and the following problems exist: the steps of the whole chromatography process are complex, manual switching is needed among the steps, and if the switching is not timely, the resin column is drained, so that the whole purification unit is influenced; under the condition of limited experimenters, the simultaneous optimization of a plurality of sets of chromatography experimental schemes is difficult to realize, and the project progress is influenced.
Disclosure of Invention
The utility model aims at overcoming the weak point among the prior art, providing an automatic chromatography device, the device simple structure, can accomplish the chromatography appearance automatically, wash miscellaneous, elution, regeneration process, need not the chromatographic system of experimenter completion purification process in the field.
Specifically, the utility model adopts the following technical scheme to realize the above-mentioned purpose.
The utility model provides an automatic chromatography device, the device comprises a feeding tank group, a sample injection controller, a feeding pump, a chromatography column body, an outflow liquid controller and a collecting tank group, wherein the feeding tank group comprises a sample feeding liquid tank, a impurity washing liquid tank, an elution liquid tank, a regeneration liquid tank and a purification water tank, the collecting tank group comprises 3 tanks for respectively collecting concentrated wastewater, clear wastewater and elution liquid, the feeding tank group is respectively connected into one end of the sample injection controller through a silica gel hose, the other end of the sample injection controller is connected with one end of the feeding pump, the other end of the feeding pump is connected with one end of the chromatography column body, feed liquid is pumped into the chromatography column body through the feeding pump, the other end of the chromatography column is connected with one end of the outflow liquid controller, the outflow liquid controller is provided with a multi-position multi-way electromagnetic valve in a pipeline connected with the chromatography column body, the other end of the effluent controller is connected into the collection tank group through a hose.
The sample injection controller is a set of automatic controller, and can control the switching of pipelines in different periods such as sample loading, impurity washing, elution and regeneration according to flow in a timed and quantitative manner.
The feeding pump is selected according to different chromatographic columns and fillers and comprises but is not limited to a diaphragm pump and a peristaltic pump.
The chromatography column is made of materials including but not limited to PP, glass or stainless steel materials.
The chromatography column can be a medium-low pressure column or a high-pressure column according to different filling amounts and types of the fillers.
The effluent controller is a set of automatic controller, and can control the switching of pipelines in different periods such as sampling, impurity washing, elution and regeneration according to flow timing and quantification.
The utility model has the advantages that: the traditional column chromatography purification process needs manual switching of the processes of sample loading, impurity washing, elution, regeneration and the like. Although the workload is not great, the switching period needs to be closely attended by people, otherwise, dry columns can be caused, and the risk of influencing the whole purification process is possibly caused. Automatic chromatography system introduced automatic control system on traditional chromatography device's basis. Sample loading liquid, impurity washing liquid, eluent and regeneration liquid are prepared in advance, and after a sample injection controller and an effluent liquid controller are set, the sample loading, impurity washing, elution and resin regeneration can be automatically completed under unattended operation. The chromatographic experiment can be conveniently carried out by utilizing off-duty time, and the chromatographic process optimization time can be greatly shortened and the project development progress can be shortened under the condition that a plurality of groups of chromatographic systems are matched for use.
Drawings
Fig. 1 is a schematic structural diagram of the automatic chromatography device of the present invention. The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the principles of the invention.
In the figure: 1-a feeding tank group, 2-a sample introduction controller, 3-a feeding pump, 4-a chromatography column, 5-an effluent controller and 6-a collection tank group.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the embodiments and drawings of the present invention are combined to clearly and completely describe the technical solutions of the present invention.
Example 1
As shown in figure 1, the utility model provides an automatic chromatography device, which comprises a feeding tank group 1, a sample injection controller 2, a feeding pump 3, a chromatography column 4, an effluent controller 5 and a collecting tank group 6, wherein the feeding tank group 1 comprises a sample feeding tank, a impurity washing tank, an elution tank, a regeneration tank and a purification water tank, the collecting tank group 6 comprises 3 tanks for respectively collecting concentrated wastewater, clear wastewater and elution, the feeding tank group 1 is respectively connected into one end of the sample injection controller 2 through a silica gel hose, the other end of the sample injection controller 2 is connected with one end of the feeding pump 3, the other end of the feeding pump 3 is connected with one end of the chromatography column 4, feed liquid is pumped into the chromatography column 4 through the feeding pump 3, the other end of the chromatography column 4 is connected with one end of the effluent controller 5, the effluent controller 5 and a multi-way electromagnetic valve is arranged in a pipeline connected with the chromatography column 4, the other end of the effluent controller 5 is connected into a collection tank group 6 through a hose.
The sample injection controller 2 is a set of automatic controller, and can control the switching of pipelines in different periods such as sample loading, impurity washing, elution, regeneration and the like according to flow in a timing and quantitative manner.
The feeding pump 3 is selected according to different chromatographic columns and packing materials, and includes but is not limited to a diaphragm pump and a peristaltic pump.
The chromatography column 4 is made of materials including but not limited to PP, glass or stainless steel.
The chromatographic column 4 can be a medium-low pressure column or a high-pressure column according to different filling amounts and types of the filling materials.
The effluent controller 5 is a set of automatic controller and can control the switching of pipelines in different periods such as sampling, impurity washing, elution, regeneration and the like according to the flow in a timing and quantitative manner.
Example 2 application
The utility model provides an automatic chromatography device which is applied to purifying a certain antibiotic by macroporous resin, as shown in figure 1, a feeding tank group 1 consists of a sample feeding liquid tank, a impurity washing liquid tank, an elution liquid tank, a regeneration liquid tank and a purification water tank, the feeding tank group is respectively connected into a sample injection controller 2 through silica gel hoses, the automatic switching of each pipeline is realized by setting the switching time of each outlet of the electromagnetic valve for the matched software of the sample injection controller 2, then connecting with a feed pump 3, connecting with a chromatography column 4 filled with 1L macroporous resin, allowing the effluent passing through the chromatography column 4 to enter an effluent controller 5 through a pipeline, each outlet switching time of the electromagnetic valve is set through matched software of the effluent liquid controller 5 so as to realize automatic switching of each pipeline, effluent liquid enters the collection tank set 6 respectively after automatic switching, and the collection tank set 6 consists of a waste water tank and eluent collection tanks 1-5. The flow rate of a feed pump is set to be 1L/h, the sample loading time of the sample injection controller 2 is adjusted to be 8h, the impurity washing time is adjusted to be 4h, the elution time is adjusted to be 6h, the regeneration time is adjusted to be 4h, the purification water washing time is adjusted to be 4h, the eluent is collected to the elution collecting tank 1 by the outflow liquid controller 5, the eluent is collected to the collecting tank 2 after 1h, and the rest wastewater is collected to the wastewater tank until the elution is completed. And after 26h, automatically completing purification of the antibiotics, collecting the antibiotics into a 1-5 collection tank, and after HPLC (high performance liquid chromatography) detection, improving the purity of the purified antibiotics from 75% to 89%, wherein the yield is 80%.
In this embodiment, the laboratory technician only needs to prepare the sample loading liquid, the impurity washing liquid, the eluent and the regeneration liquid in the corresponding tank, and then the whole purification process can be automatically completed by the automatic chromatography device after the residual 26 hours, and the purified sample enters the next purification step. The idle time is utilized to the maximum extent to complete the purification process, so that the optimization time of the chromatography process can be greatly shortened, and the project development progress is shortened.
The above-mentioned embodiments are only used for explaining the inventive concept of the present invention, and do not limit the scope of the present invention. It should be understood by those skilled in the art that modifications and variations made based on the disclosure of the present invention are within the scope of the invention.
Claims (4)
1. An automatic chromatography device, which is characterized in that the device comprises a feeding tank group, a sample injection controller, a feeding pump, a chromatography column body, an effluent controller and a collecting tank group, wherein the feeding tank group consists of a sample feeding liquid tank, a impurity washing liquid tank, an elution liquid tank, a regeneration liquid tank and a purification water tank, the collecting tank group consists of 3 tanks and is used for respectively collecting concentrated wastewater, clear wastewater and eluent, the feeding tank group is respectively connected into one end of the sample injection controller through a silica gel hose, the other end of the sample injection controller is connected with one end of the feeding pump, the other end of the feeding pump is connected with one end of the chromatography column body, feed liquid is pumped into the chromatography column body through the feeding pump, the other end of the chromatography column is connected with one end of the effluent controller, and a multi-position and multi-way electromagnetic valve is arranged in a pipeline connected with the chromatography column body, the other end of the effluent controller is connected into the collection tank group through a hose,
the sample injection controller is a set of automatic controller, can control the switching of pipelines at different periods such as sample loading, impurity washing, elution, regeneration and the like in a timed and quantitative manner according to the flow,
the effluent controller is a set of automatic controller, and can control the switching of pipelines in different periods such as sampling, impurity washing, elution and regeneration according to flow timing and quantification.
2. The automated chromatography apparatus of claim 1, wherein the feed pump is a diaphragm pump or a peristaltic pump.
3. The automated chromatography device of claim 1, wherein the chromatography column is made of PP, glass or stainless steel.
4. The automated chromatography apparatus of claim 1, wherein the chromatography column is a medium-low pressure column or a high-pressure column.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020636525.5U CN212467209U (en) | 2020-04-24 | 2020-04-24 | Automatic change chromatography device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020636525.5U CN212467209U (en) | 2020-04-24 | 2020-04-24 | Automatic change chromatography device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212467209U true CN212467209U (en) | 2021-02-05 |
Family
ID=74453869
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020636525.5U Active CN212467209U (en) | 2020-04-24 | 2020-04-24 | Automatic change chromatography device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212467209U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116392854A (en) * | 2023-06-07 | 2023-07-07 | 江中药业股份有限公司 | Full-automatic chromatographic device and analysis system |
-
2020
- 2020-04-24 CN CN202020636525.5U patent/CN212467209U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116392854A (en) * | 2023-06-07 | 2023-07-07 | 江中药业股份有限公司 | Full-automatic chromatographic device and analysis system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101678244B (en) | Method and device for continuous membrane adsorption | |
| KR101152036B1 (en) | A method to improve the sorbent efficiency of protein A chromatography using switching column with continuous feeding | |
| WO2012074481A1 (en) | System and process for biopolymer chromatography | |
| CN107485891B (en) | Improved chromatographic device and method for continuous flow chromatography | |
| CN109030686B (en) | Three-dimensional liquid chromatography protein purification device and use method thereof | |
| CN212467209U (en) | Automatic change chromatography device | |
| CN109030647B (en) | Online immunoaffinity purification detection device for terbutaline, salbutamol, ractopamine and clenbuterol | |
| CN213853215U (en) | Multi-column continuous sample loading and purifying device | |
| CN215415250U (en) | Production type circulating multidimensional liquid chromatography separation system | |
| CN213357700U (en) | Continuous ion exchange device for chromatographic separation and gallium extraction of aluminum chloride solution | |
| CN215415249U (en) | Production type circulating multidimensional liquid chromatography separation system | |
| CN201589735U (en) | On-line multi-channel immediate solid-phase extraction device | |
| CN209215064U (en) | A kind of separation of close and distant water component and collection device based on on-line checking | |
| JP2011214837A (en) | Liquid chromatography apparatus | |
| CN215575173U (en) | Continuous flow chromatography system | |
| CN212159315U (en) | Device for automatically enriching red tide toxins in concentrated seawater | |
| CN203447822U (en) | Chromatographic column suitable for 96-well tissue culture plate | |
| CN106018613A (en) | Solid-phase extraction device capable of realizing multi-column series connection through online switching and extraction method of solid-phase extraction device | |
| CN203519377U (en) | Automatic sampling device for water treatment adsorption experiment | |
| CN219150153U (en) | Resin column elution device | |
| CN209797824U (en) | Automatic purification system | |
| CN216170113U (en) | Instrument for realizing automatic affinity chromatography | |
| CN110286174A (en) | A kind of online highly effective liquid phase chromatographic device of multidimensional | |
| CN220393592U (en) | Protein purification device capable of regulating flushing flow rate | |
| CN110286175A (en) | A kind of multidimensional on-line solid phase extraction liquid-chromatography apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |