CN203102052U - A device for maintaining a constant sample temperature during microscopic observation - Google Patents

Abstract

The utility model discloses a device for maintaining a constant sample temperature during microscopic observation. The device includes a flexible insulating material, a heating electric heating wire, a temperature measuring thermal resistance, a data acquisition module, and a communication module. The device is covered with a flexible insulating material. The electric heating wire is heated, and the temperature signal of the flexible insulating material is collected by the thermal resistance sensor, and the temperature signal is sent to the control computer to form a negative feedback, and the computer feedback signal is used to control and maintain the temperature of the sample at a constant level. In use, the device surrounds the sample dish and controls the temperature of the sample in the center of the sample dish by exchanging heat with the sample dish; the device can realize any temperature constant within the temperature range of 25-100°C; the device can be connected by a soft connection buckle , for easy wrapping of sample vessels.

Description

A device for maintaining a constant sample temperature during microscopic observation

technical field

The utility model belongs to the field of analysis and testing instruments, and in particular relates to a device for maintaining a constant sample temperature during microscopic observation. power to maintain a constant temperature of the sample in the sample vessel. the

Background technique

In the process of chemical and chemical production and scientific research, it is often necessary to observe the microscopic optical morphology of samples at different temperatures in real time and online through a microscope. Although traditional water baths, electric heating mantles, incubators, etc. can control the temperature of samples to be constant, they cannot realize real-time and online observation of samples through microscopes. At present, there is a temperature-changing device specially used for microscopes produced by INSTEC on the market, which can realize continuous temperature changes from -78°C to 250°C, but its operation is complicated and the equipment is expensive, which cannot be afforded by ordinary laboratories. In addition, in view of its own design, this temperature-changing device has a biggest shortcoming: the microscope can only be observed with a long working distance objective lens, and the higher the multiple of the objective lens, the shorter the working distance. Unable to find samples using this variable temperature device. Therefore, the widespread use of this temperature changing device is greatly limited. On the market, Tokai Hit Company has produced a CO ₂ incubation device, which is used to maintain biological samples such as cells at 37°C, the CO ₂ content is kept at 5%, and the humidity is 90%. This device is very suitable for making biological samples, but it is also expensive, complicated to operate, and requires a _CO2 cylinder, which occupies a large area. The most important thing is that this device needs to be matched with a suitable stage, and it is not suitable for all optical microscopes. For chemical samples, it is often necessary to analyze the optical characteristics of the sample at different temperatures, and for cell samples, temperature is the most important relative to humidity and _CO2 content, as long as the temperature is maintained at 37 ° C, the survival time of cells is very fast Will be greatly increased than at room temperature 25°C. In addition, the domestic heating device mainly heats the round tray of the sample. Although direct heating can be achieved in this way, since the circular sample tray is directly connected to the stage, and the material of the stage is generally plastic, it cannot withstand high temperature. Therefore, long-term use will damage the stage, thereby affecting the connection between the stage and the sample tray.

Therefore, the purpose of this utility model is to provide a heating device that can use common objective lenses, is safe to the stage, and can be widely used in common optical microscopes to maintain a constant sample temperature. the

Utility model content

In order to overcome the above-mentioned defects, the utility model designs a simple device for maintaining a constant temperature of the sample, which can control the temperature in real time and on-line during microscopic observation. the

The sample dish referred to in the utility model is a cylindrical carrier dish made of aluminum and a cover glass, which is similar to a confocal dish for culturing cells. The purpose of this utility model is to propose a device for maintaining a constant sample temperature during microscopic observation. The device includes a flexible insulating material 2, a heating heating wire 3, a temperature measuring thermal resistance 4, a data acquisition module 5, and a communication module 6, wherein ,

The heating wire 3 is wrapped inside the flexible insulating material 2;

The temperature-measuring thermal resistor 4 is placed at the center of the flexible insulating material 2 for measuring the temperature of the flexible insulating material 2 and generating a temperature signal;

The data collection module 5 collects the temperature signal and transmits the temperature signal to the control computer through the communication module 6 . the

The device also includes a DA output module 7, the DA output module 7 receives the feedback signal sent by the control computer through the communication module 6, and controls the heating rate of the heating wire 3 according to the feedback signal. the

The device also includes a connecting buckle 8 for connecting the device with the sample dish so as to fix the device on the sample dish, so that the temperature of the sample at the center of the sample dish can be controlled through heat exchange with the sample dish. the

The connecting button 8 is a soft connecting button. the

The DA output module 7 controls the heating rate of the heating heating wire 3 by controlling the power of the heating heating wire 3 . the

There is a corresponding relationship between the actual temperature t _real of the sample in the sample dish and the temperature t measured by the temperature measuring thermal _resistor 4 .

The control computer generates a feedback signal according to the temperature t measured by _{the temperature measuring thermal resistance 4 and the preset target temperature T to be reached by the sample} to control the heating temperature of the device.

The feedback signal is a feedback signal for increasing/decreasing the power of the heating wire 3 . the

With the device designed by the utility model, real-time and online observation of the optical morphology of samples at different temperatures can be realized on ordinary microscopes, fluorescence microscopes, confocal microscopes, two-photon microscopes and other instruments. Common objective lenses can be used, safe for the stage. Moreover, the device is simple and cheap, and is helpful for popularization in various laboratories. the

Description of drawings

Fig. 1 is a structural schematic diagram and a working principle diagram of a flexible thermostat, a device for maintaining a constant temperature of a sample proposed by the utility model. the

Fig. 2 is a schematic diagram of the connection between the flexible thermostat shown in Fig. 1 and the sample vessel. the

Detailed ways

In order to make the purpose, technical solutions and advantages of the utility model clearer, the utility model will be further described in detail below in combination with specific embodiments and with reference to the accompanying drawings. the

The utility model provides a device for maintaining a constant temperature of a sample, especially a device for maintaining a constant temperature of a sample during microscopic observation. Fig. 1 is a schematic diagram of the structure and working principle of the device for maintaining a constant temperature of a sample - a flexible thermostat , in the figure: 1 is the whole flexible thermostat, 2 is the flexible insulating material, 3 is the heating wire, 4 is the temperature measuring thermal resistance, 5 is the data acquisition module, 6 is the communication module, 7 is the DA output module, 8 is the connection buckle. The flexible thermostat 1 shown in the figure is mainly composed of a flexible insulating material 2 , a heating wire 3 , a temperature measuring thermal resistance 4 , a data acquisition module 5 , a communication module 6 , a DA output module 7 and a connecting button 8 . the

The heating wire 3 wrapped inside the flexible insulating material 2 is used to heat the sample vessel, and a temperature-measuring thermal resistor 4 is installed in the center of the flexible insulating material to measure the temperature of the flexible insulating material, that is, the temperature of the flexible thermostat, and generate a temperature signal, data The acquisition module 5 collects the temperature signal and transmits it to the control computer through the communication module 6; the DA output module 7 receives the feedback signal sent by the control computer through the communication module 6, and controls the heating of the heating heating wire 3 according to the feedback signal rate. The function of the connecting buckle 8 is to fasten the flexible thermostat and the sample vessel so as to fix the flexible thermostat on the sample vessel. the

In fact, there is a difference between the actual temperature t _real of the sample in the sample dish and the temperature t real measured by the thermal resistance of the flexible thermostat. The actual temperature t _real of the sample can be directly measured by a _thermometer , and what we can control is the flexible thermostat The temperature t _thermostat . Within a certain temperature range, determine the temperature of the flexible thermostat, that is, the corresponding relationship between the temperature t _thermostat measured by the thermal resistance and the actual temperature t _real of the sample, and fit it into a function. In this way, when the sample is about to reach a certain temperature _treal , the temperature _treal that the flexible thermostat needs to reach can be determined according to the above function.

The control computer controls the heating temperature required by the flexible thermostat according to the preset temperature that the actual sample needs to reach according to the program designed based on heat transfer, and gives a feedback signal, which is transmitted to the DA through the communication module 6 The output module 7 uses the DA output module 7 to control the power of the heating heating wire to control the heating rate of the heating heating wire 3 . the

The above functions are realized by a computer-controlled heating program. The specific process is as follows: To make the sample reach the target temperature T required by the experiment, it is necessary to know the difference between the target temperature T and the actual temperature t _real of the sample at that time to determine the heating program of the flexible thermostat. When the ambient temperature and the sample target temperature T are constant, there is a one-to-one correspondence between the temperature t measured by the thermal resistance of the flexible _thermostat and the actual temperature t _real of the sample, which can be fitted into a function. When the actual temperature t _real of the sample is lower than the desired target temperature T, when the difference between the target temperature T and the actual temperature t _real of the sample corresponding to the temperature t measured by the thermal resistance of the flexible _thermostat is greater than a certain Δt , the calculation program gives a feedback signal, and continues heating by controlling the _power of the flexible thermostat to heat the heating wire 3; The feedback signal of the power of the heating heating wire 3 is used to reduce the heating rate by controlling the power value of the heating heating wire 3 of the flexible thermostat, so that the heating heating wire 3 is slowly heated to the target temperature T required by the sample; when the actual temperature t _real of the sample is high In the case of the target temperature T that the sample wants to reach, and the difference is less than a certain Δt', the control computer sends a feedback signal to reduce the power of the heating heating wire 3 to reduce the heating rate, so that the heating heating wire 3 is slowly heated until When the difference between the target temperature T and the actual temperature t _real is greater than or equal to a certain Δt′, the heating is stopped. In this way, the sample temperature can be controlled to achieve the target temperature T required by the user.

In actual use, the flexible thermostat surrounds the sample vessel. Preferably, the flexible thermostat is connected to the sample vessel through a soft connection buckle 8 to facilitate wrapping the sample vessel. Figure 2 shows the connection relationship between the flexible thermostat and the sample dish, in which 9 is the sample dish. The flexible thermostat surrounding the sample dish 9 exchanges heat with the sample dish 9 to finally realize the control of the temperature of the sample in the center of the sample dish 9 . Through the above-mentioned device provided by the utility model, the temperature of the sample can be conveniently controlled until reaching the target temperature T required by the user. the

The device for maintaining a constant sample temperature proposed by the utility model can realize that the sample maintains a constant temperature at any temperature within the temperature range of 25-100°C. the

The specific embodiments described above have further described the purpose, technical solutions and beneficial effects of the present utility model in detail. It should be understood that the above descriptions are only specific embodiments of the present utility model and are not intended to limit the present invention. For the utility model, any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the utility model shall be included in the protection scope of the utility model. the

Claims (8)

1. keep the constant device of sample temperature during a microexamination, it is characterized in that, described device comprises flexible insulating material (2), heating heating wire (3), thermometric thermal resistance (4), data acquisition module (5), communication module (6), wherein,

Described heating heating wire (3) is wrapped in described flexible insulating material (2) inside;

Described thermometric thermal resistance (4) places the center of described flexible insulating material (2), is used to measure the temperature of described flexible insulating material (2), and produces a temperature signal;

Described data acquisition module (5) is gathered described temperature signal and by communication module (6) described temperature signal is transferred to the control computer heating wire.

2. device according to claim 1, it is characterized in that, described device also comprises DA output module (7), described DA output module (7) receives the feedback signal that described control computer sends over by described communication module (6), and controls the rate of heat addition of described heating heating wire (3) according to described feedback signal.

3. device according to claim 1, it is characterized in that described device also comprises a junction button (8), is used to connect described device and planchet, so that described device is fixed on the planchet, thus by with the final control that realizes planchet center sample temperature of the heat exchange of planchet.

4. device according to claim 3 is characterized in that, described junction button (8) is the button that is flexible coupling.

5. device according to claim 2 is characterized in that, described DA output module (7) is controlled the rate of heat addition of described heating heating wire (3) by the watt level of the described heating heating wire of control (3).

6. device according to claim 3 is characterized in that, the actual temperature t of sample in the described planchet _RealThe temperature t that records with described thermometric thermal resistance (4) _ThermostatThere is a corresponding relation.

7. device according to claim 1 is characterized in that, the temperature t that described control computer records according to described thermometric thermal resistance (4) _ThermostatAnd the target temperature T that predefined sample need reach generates a feedback signal, controls the heating-up temperature of described device.

8. according to claim 2 or 7 described devices, it is characterized in that, described feedback signal for to increase/reduce the feedback signal of the power of described heating heating wire (3).

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