CN203102052U - Apparatus for maintaining sample temperature constancy in microscopic observation processes - Google Patents
Apparatus for maintaining sample temperature constancy in microscopic observation processes Download PDFInfo
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- CN203102052U CN203102052U CN 201320043823 CN201320043823U CN203102052U CN 203102052 U CN203102052 U CN 203102052U CN 201320043823 CN201320043823 CN 201320043823 CN 201320043823 U CN201320043823 U CN 201320043823U CN 203102052 U CN203102052 U CN 203102052U
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Abstract
The utility model discloses an apparatus for maintaining sample temperature constancy in microscopic observation processes. The apparatus comprises a flexible insulating material, an electric heating wire, a temperature measurement thermal resistor, a data acquisition module and a communication module, wherein the device is heated by using the electric heating wire coated by the flexible insulating material, temperature signals of the flexible insulating material are collected through a thermal resistance sensor, the temperature signals are transmitted to a control computer and negative feedback is formed, and sample temperature constancy can be controlled and maintained through computer feedback signals. In utilization processes, the apparatus surrounds a sample vessel. Temperature of a sample at the center of the vessel can be controlled through heat exchange between the apparatus and the sample vessel. The apparatus can realize constancy of any temperature within the temperature scope of 25 to 100 DEG C, the apparatus can be connected through a flexible connection buckle, and therefore the sample vessel can be wrapped conveniently.
Description
Technical field
The utility model belongs to the analytical and testing instrument field, keep the constant device of sample temperature when relating in particular to a kind of microexamination, specifically, the utility model heats the heating wire that adopts flexible insulating material to coat, and keeps the temperature constant of sample in the planchet by the power of regulating the heating heating wire.
Background technology
In chemical production and scientific research process, often need by microscope in real time, the microcosmic optical morphology during the observation sample different temperatures online.Though traditional water-bath, electric jacket, constant temperature oven etc. can be controlled the temperature constant of sample, can't realize by microscope in real time, observation sample online.The microscopical changing device that is exclusively used in that INSTEC company produces is arranged in the market, can realize temperature-78 ℃-250 ℃ continuous variation, but its complicated operation, apparatus expensive, common laboratory are held and are dared not accept.In addition, this changing device has the deficiency of a maximum in view of the design of himself: microscope is only to observe with the long reach object lens, the object lens multiple is high more, operating distance is short more, for 100 times object lens, even the long reach object lens also can't utilize this changing device to find sample.Therefore, greatly limited being extensive use of of this changing device.Tokai Hit company has produced a kind of CO on the market
2Hatch device, be used for biological sample such as cell etc. being maintained 37 ℃, CO
2Content remains on 5%, humidity 90%.This device is fit to do biological sample very much, but price is also very expensive, and complicated operation also needs CO
2Steel cylinder, floor area is big.The most important thing is that this device needs suitable objective table coupling, and be not suitable for all optical microscopes.For chemical example, often need to analyze be different temperatures the time sample optical signature, and for cell sample, with respect to humidity and CO
2Content, temperature are most important, as long as temperature maintenance is at 37 ℃, the time-to-live of cell will be than increasing greatly under 25 ℃ of room temperatures.In addition, homemade heating arrangement mainly is that round pallet to sample heats.Though can realize direct heating like this, owing to the circular sample pallet directly is connected with objective table, and the material of objective table generally all is plastics, can not bear high temperature.Therefore, long-time use can diminish objective table, and then influences being connected of objective table and sample tray.
Therefore, the purpose of this utility model provides and a kind ofly uses common object lens, to objective table safety, and can be widely used in ordinary optical microscope keep the constant heating arrangement of sample temperature.
The utility model content
In order to overcome above-mentioned defective, the utility model has designed a kind of constant device of sample temperature of simply keeping, and during microexamination, can control temperature in real time, online.
The planchet of the utility model indication is the cylindric loading ware made from aluminium and cover glass, is similar to the confocal ware of cultured cell.The purpose of this utility model is to keep the constant device of sample temperature when proposing a kind of microexamination, and described device comprises flexible insulating material 2, heating heating wire 3, thermometric thermal resistance 4, data acquisition module 5, communication module 6, wherein,
Described heating heating wire 3 is wrapped in described flexible insulating material 2 inside;
Described thermometric thermal resistance 4 places the center of described flexible insulating material 2, is used to measure the temperature of described flexible insulating material 2, and produces a temperature signal;
Described data acquisition module 5 is gathered described temperature signal and by communication module 6 described temperature signal is transferred to control computer.
Described device also comprises DA output module 7, and described DA output module 7 receives the feedback signal that described control computer sends over by described communication module 6, and controls the rate of heat addition of described heating heating wire 3 according to described feedback signal.
Described device also comprises a junction button 8, is used to connect described device and planchet, so that described device is fixed on the planchet, thereby by with the final control that realizes planchet center sample temperature of the heat exchange of planchet.
Described junction button 8 is the button that is flexible coupling.
Described DA output module 7 is controlled the rate of heat addition of described heating heating wire 3 by the watt level of controlling described heating heating wire 3.
The actual temperature t of sample in the described planchet
RealThe temperature t that records with described thermometric thermal resistance 4
ThermostatThere is a corresponding relation.
The temperature t that described control computer records according to described thermometric thermal resistance 4
ThermostatAnd the target temperature T that predefined sample need reach generates a feedback signal, controls the heating-up temperature of described device.
Described feedback signal is the feedback signal that increases/reduce the power of described heating heating wire 3.
Utilize the device of the utility model design, can on instruments such as simple microscope, fluorescent microscope, Laser Scanning Confocal Microscope and two-photon microscope, realize in real time, the optical morphology of observation sample different temperatures online.Can use common object lens, to objective table safety.And this device is simple, cheap, helps the popularization in each laboratory.
Description of drawings
Fig. 1 is structural representation and the fundamental diagram of keeping the constant device of sample temperature one flexible thermostat that the utility model proposes.
Fig. 2 is the flexible thermostat shown in Figure 1 and the connection diagram of planchet.
Embodiment
For making the purpose of this utility model, technical scheme and advantage clearer,, and, the utility model is further described with reference to accompanying drawing below in conjunction with specific embodiment.
The utility model provides a kind of constant device of sample temperature of keeping, keep the constant device of sample temperature during especially a kind of microexamination, Fig. 1 is described structural representation and the fundamental diagram of keeping the constant device of sample temperature-flexible thermostat, and among the figure: 1 is whole flexible thermostat, and 2 is flexible insulating material, 3 are the heating heating wire, 4 is the thermometric thermal resistance, and 5 is data acquisition module, and 6 is communication module, 7 is the DA output module, and 8 is junction button.Flexible thermostat 1 shown in the figure mainly is made up of flexible insulating material 2, heating heating wire 3, thermometric thermal resistance 4, data acquisition module 5, communication module 6, DA output module 7 and junction button 8.
Flexible insulating material 2 internal package heating heating wire 3 is used for the heated sample ware, thermometric thermal resistance 4 is housed to measure the temperature of flexible insulating material at the flexible insulating material center, it is the temperature of flexible thermostat, and producing a temperature signal, data acquisition module 5 is gathered described temperature signal and is transferred to control computer by communication module 6; DA output module 7 receives the feedback signal that control computer sends over by communication module 6, and controls the rate of heat addition of described heating heating wire 3 according to described feedback signal.The effect of junction button 8 is to be used for flexible thermostat of fastening and planchet, so that flexible thermostat is fixed on the planchet.
In fact, the actual temperature t of sample in the planchet
RealThe temperature t that records with flexible thermostat thermal resistance
ThermostatBe differentiated, sample actual temperature t
RealCan directly measure by thermometer, be the temperature t of flexible thermostat and we can control
ThermostatIn certain temperature range, determine the flexible thermostat temperature temperature t that records of thermal resistance just
ThermostatWith sample actual temperature t
RealCorresponding relation, fit to a function.Like this, to reach some temperature t when sample
RealThe time, just can determine the required temperature t that reaches of flexible thermostat according to above-mentioned function
Thermostat
Control computer is according to the program based on the thermal conduction study design, control the required heating-up temperature of flexible thermostat according to the temperature that predefined actual sample needs reach, and provide a feedback signal, this feedback signal transfers to DA output module 7 through communication module 6, thereby utilizes the rate of heat addition of the watt level control heating heating wire 3 of DA output module 7 control heating heating wire.
Above-mentioned functions realizes that by computer-controlled heating schedule detailed process is as follows: make sample reach the target temperature T of requirement of experiment, need know target temperature T and sample actual temperature t at that time
RealDifference decide the heating schedule of flexible thermostat.Under the certain situation of environment temperature, sample target temperature T, measure temperature t by the thermal resistance of flexible thermostat
ThermostatWith sample actual temperature t
RealOne-to-one relationship is arranged, can fit to function.Actual temperature t at sample
RealBe lower than under the situation of wishing the target temperature T reach, when target temperature T and thermal resistance measurement temperature t by flexible thermostat
ThermostatCorresponding sample actual temperature t
RealDifference during greater than certain Δ t, calculation procedure provides feedback signal, by controlling the power of flexible oven heated heating wire 3, continues heating; As actual temperature t
RealWhen being increased to the difference of target temperature T smaller or equal to certain Δ t, computing machine sends the feedback signal of the power that reduces to heat heating wire 3, by controlling the performance number of flexible oven heated heating wire 3, reduce the rate of heat addition, make heating heating wire 3 slowly be heated to the target temperature T that sample needs; As sample actual temperature t
RealBe higher than under the target temperature T situation that sample wishes to reach, and difference is less than certain Δ t ' time, and control computer is sent the feedback signal of the power that reduces to heat heating wire 3, to reduce the rate of heat addition, heating heating wire 3 is slowly heated, until target temperature T and described actual temperature t
RealDifference more than or equal to certain Δ t ' time, stop the heating.By this method, can control sample temperature and reach the required target temperature T of user.
In actual use, described flexible thermostat is looped around around the planchet, and preferably, flexible thermostat connects planchet by the button 8 that is flexible coupling, with convenient parcel planchet.Fig. 2 has provided the annexation of flexible thermostat and planchet, and among the figure, 9 is planchet.Be looped around planchet 9 flexible thermostat on every side by carrying out heat exchange, with the control of final realization to planchet 9 center sample temperatures with planchet 9.By the said apparatus that the utility model provides, the temperature that can control sample easily is until reaching the required target temperature T of user.
The utility model proposed keep the constant device of sample temperature can realize sample in 25～100 ℃ of temperature ranges on arbitrary temperature holding temperature constant.
Above-described specific embodiment; the purpose of this utility model, technical scheme and beneficial effect are further described; institute is understood that; the above only is a specific embodiment of the utility model; be not limited to the utility model; all within spirit of the present utility model and principle, any modification of being made, be equal to replacement, improvement etc., all should be included within the protection domain of the present utility model.
Claims (8)
1. keep the constant device of sample temperature during a microexamination, it is characterized in that, described device comprises flexible insulating material (2), heating heating wire (3), thermometric thermal resistance (4), data acquisition module (5), communication module (6), wherein,
Described heating heating wire (3) is wrapped in described flexible insulating material (2) inside;
Described thermometric thermal resistance (4) places the center of described flexible insulating material (2), is used to measure the temperature of described flexible insulating material (2), and produces a temperature signal;
Described data acquisition module (5) is gathered described temperature signal and by communication module (6) described temperature signal is transferred to the control computer heating wire.
2. device according to claim 1, it is characterized in that, described device also comprises DA output module (7), described DA output module (7) receives the feedback signal that described control computer sends over by described communication module (6), and controls the rate of heat addition of described heating heating wire (3) according to described feedback signal.
3. device according to claim 1, it is characterized in that described device also comprises a junction button (8), is used to connect described device and planchet, so that described device is fixed on the planchet, thus by with the final control that realizes planchet center sample temperature of the heat exchange of planchet.
4. device according to claim 3 is characterized in that, described junction button (8) is the button that is flexible coupling.
5. device according to claim 2 is characterized in that, described DA output module (7) is controlled the rate of heat addition of described heating heating wire (3) by the watt level of the described heating heating wire of control (3).
6. device according to claim 3 is characterized in that, the actual temperature t of sample in the described planchet
RealThe temperature t that records with described thermometric thermal resistance (4)
ThermostatThere is a corresponding relation.
7. device according to claim 1 is characterized in that, the temperature t that described control computer records according to described thermometric thermal resistance (4)
ThermostatAnd the target temperature T that predefined sample need reach generates a feedback signal, controls the heating-up temperature of described device.
8. according to claim 2 or 7 described devices, it is characterized in that, described feedback signal for to increase/reduce the feedback signal of the power of described heating heating wire (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320043823 CN203102052U (en) | 2013-01-25 | 2013-01-25 | Apparatus for maintaining sample temperature constancy in microscopic observation processes |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201320043823 CN203102052U (en) | 2013-01-25 | 2013-01-25 | Apparatus for maintaining sample temperature constancy in microscopic observation processes |
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| CN203102052U true CN203102052U (en) | 2013-07-31 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106367344A (en) * | 2016-08-27 | 2017-02-01 | 上海交通大学 | Cell micro-scale heating culture control device |
| CN109582059A (en) * | 2018-11-30 | 2019-04-05 | 中国航空工业集团公司洛阳电光设备研究所 | A kind of optical element temprature control method and optical system |
| CN110118618A (en) * | 2018-02-05 | 2019-08-13 | 浙江智柔科技有限公司 | Thermostatic equipment |
-
2013
- 2013-01-25 CN CN 201320043823 patent/CN203102052U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106367344A (en) * | 2016-08-27 | 2017-02-01 | 上海交通大学 | Cell micro-scale heating culture control device |
| CN106367344B (en) * | 2016-08-27 | 2019-07-05 | 上海交通大学 | A kind of cell minute yardstick heating culture control device |
| CN110118618A (en) * | 2018-02-05 | 2019-08-13 | 浙江智柔科技有限公司 | Thermostatic equipment |
| CN109582059A (en) * | 2018-11-30 | 2019-04-05 | 中国航空工业集团公司洛阳电光设备研究所 | A kind of optical element temprature control method and optical system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130731 Termination date: 20150125 |
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| EXPY | Termination of patent right or utility model |