CN103135632A

CN103135632A - Device for keeping temperature of samples constant while at time of microexamination

Info

Publication number: CN103135632A
Application number: CN2011103862073A
Authority: CN
Inventors: 刘美蓉; 丁丽萍
Original assignee: Institute of Chemistry CAS
Current assignee: Institute of Chemistry CAS
Priority date: 2011-11-29
Filing date: 2011-11-29
Publication date: 2013-06-05
Anticipated expiration: 2031-11-29
Also published as: CN103135632B

Abstract

The invention discloses a device for keeping temperature of samples constant while at the time of microexamination. The device comprises flexible insulation materials 2, a resistive heater3, a temperature thermocouple 4, a data acquisition module 5, a communication module 6 and a DA output module 7. Electric resistance wire covered by the flexible insulation materials are used for heating, temperature signals of the flexible insulation materials are collected through a thermocouple sensor and are sent to a control computer to form a negative feedback, computer feedback signals are sued for keeping the temperature of the samples constant. While being used, the device encircles a sample vessel; the temperature of the samples in the centre of the sample vessel is controlled by exchanging heat with the sample vessel. The device is capable of keeping any temperature constant at the temperature range of 25 DEG C-100 DEG C. The device is connected with the sample vessel through a flexible connection buckle so as to conveniently cover the sample vessel.

Description

Keep the constant device of sample temperature during microexamination

Technical field

The invention belongs to the analytical and testing instrument field, keep the constant device of sample temperature when relating in particular to a kind of microexamination, specifically, the present invention heats the resistance wire that adopts flexible insulating material to coat, and keeps the temperature constant of sample in planchet by the power of regulating resistive heater.

Background technology

In chemical production and process of scientific research, the microcosmic optical morphology in the time of often need to observing the sample different temperatures in real time, online by microscope.Although the temperature constant that traditional water-bath, electric jacket, constant temperature oven etc. can Quality controls can't realize observing in real time, online sample by microscope.The microscopical changing device that is exclusively used in that INSTEC company produces is arranged in the market, can realize temperature the continuous variation of-78 ℃-250 ℃, but its complicated operation, apparatus expensive, common laboratory are held and are dared not accept.In addition, this changing device has the deficiency of a maximum in view of the design of himself: to observe with the long reach object lens, the operating distance of general object lens is not enough to observe sample to microscope only, in view of the costliness of object lens price, common laboratory is unworthy of or only joins one or two the long reach object lens.Therefore, greatly limited being widely used of this changing device.Tokai Hit company has produced a kind of CO on the market ₂Incubating device is used for biological sample such as cell etc. being maintained 37 ℃, CO ₂Content remains on 5%, humidity 90%.This device is fit to do biological sample very much, but price is also very expensive, and complicated operation also needs CO ₂Steel cylinder, floor area is large.Another problem of this device is: this thermostat conducts heat by air, conducts heat slow, is fit to maintain some temperature-resistant, is not suitable for doing the alternating temperature experiment.For chemical example, often need to analyze be different temperatures the time sample optical signature, and for cell sample, with respect to humidity and CO ₂Content, temperature are most important, as long as temperature maintains 37 ℃, the time-to-live of cell will be than greatly increasing under 25 ℃ of room temperatures.

Summary of the invention

In order to overcome defects, the present invention has designed a kind of constant device of sample temperature of simply keeping, and during microexamination, can control in real time, online temperature.

In the bioanalysis test, cell is generally cultivated in the confocal ware, and the confocal ware also can be put chemical example certainly, is used for examining under a microscope its optical signature, and this is the planchet of indication of the present invention namely.Keep the constant device of sample temperature when the objective of the invention is to propose a kind of microexamination, it is characterized in that, described device comprises flexible insulating material 2, resistive heater 3, temperature thermocouple 4, data acquisition module 5, communication module 6, DA output module 7, wherein

Described resistive heater 3 is wrapped in described flexible insulating material 2 inside, is used for the temperature that heating improves described flexible insulating material 2;

Described temperature thermocouple 4 is placed in the center of described flexible insulating material 2, is used for measuring the temperature of described flexible insulating material 2, and produces a temperature signal;

Described data acquisition module 5 gathers described temperature signal and transfers to the control computing machine by communication module 6;

Described DA output module 7 receives controls the feedback signal that computing machine sends over by communication module 6, and controls the rate of heat addition of described resistive heater 3 according to described feedback signal.

The device that utilizes the present invention to design can realize observing in real time, online the optical morphology of sample different temperatures on the instruments such as simple microscope, fluorescent microscope, Laser Scanning Confocal Microscope and Two Photon Fluorescence.As long as object lens can bear the temperature of 25-100 ℃, just can use any object lens, irrelevant with the operating distance of object lens.And this device is simple, cheap, helps the popularization in each laboratory.

Description of drawings

Fig. 1 is structural representation and the fundamental diagram of keeping the constant device of sample temperature-flexible thermostat proposed by the invention.

Fig. 2 is flexible thermostat shown in Figure 1 and the connection diagram of planchet.

Embodiment

For making the purpose, technical solutions and advantages of the present invention clearer, below in conjunction with specific embodiment, and with reference to accompanying drawing, the present invention is described in more detail.

The invention provides a kind of constant device of sample temperature of keeping, especially keep the constant device of sample temperature during a kind of microexamination, Fig. 1 is described structural representation and the fundamental diagram of keeping the constant device of sample temperature-flexible thermostat, and in figure: 1 is whole flexible thermostat, and 2 is flexible insulating material, 3 is resistive heater, 4 is temperature thermocouple, and 5 is data acquisition module, and 6 is communication module, 7 is the DA output module, and 8 is junction button.Flexible thermostat 1 shown in figure mainly is comprised of flexible insulating material 2, resistive heater 3, temperature thermocouple 4, data acquisition module 5, communication module 6, DA output module 7 and junction button 8.

Flexible insulating material 2 internal package resistive heaters 3 are used for heating the raising temperature, temperature thermocouple 4 is housed to measure the temperature of flexible insulating material at the flexible insulating material center, it is the temperature of flexible thermostat, and producing a temperature signal, data acquisition module 5 gathers described temperature signal and transfers to the control computing machine by communication module 6; DA output module 7 receives controls the feedback signal that computing machine sends over by communication module 6, and controls the rate of heat addition of described resistive heater 3 according to described feedback signal.The effect of junction button 8 is the flexible thermostat of fastening and planchet, so that flexible thermostat is fixed on planchet.

In fact, the actual temperature t of sample in planchet _RealThe temperature t that records with flexible thermostat thermopair _ThermostatDifferentiated, sample actual temperature t _RealCan directly measure by thermometer, be the temperature t of flexible thermostat and we can control _ThermostatIn certain temperature range, determine the namely temperature t that records of thermopair of flexible thermostat temperature _ThermostatWith sample actual temperature t _RealCorresponding relation, fit to a function.Like this, to reach some temperature t when sample _RealThe time, just can determine the required temperature t that reaches of flexible thermostat according to above-mentioned function _Thermostat

Control computing machine according to the program based on the thermal conduction study design, control the required heating-up temperature of flexible thermostat according to the temperature that predefined actual sample needs reach, and provide a feedback signal, this feedback signal transfers to DA output module 7 through communication module 6, thereby the watt level of utilizing DA output module 7 to control resistive heater is controlled the rate of heat addition of resistive heater 3.

Above-mentioned functions is by computer-controlled heating schedule realization, and detailed process is as follows: make sample reach the target temperature T of requirement of experiment, need to know target temperature T and sample actual temperature t at that time _RealDifference decide the heating schedule of flexible thermostat.Actual temperature t at sample _RealIn the situation of the target temperature T that reaches lower than hope, as target temperature T and thermocouple measuring temperature t by flexible thermostat _ThermostatCorresponding sample actual temperature t _RealDifference during greater than certain Δ t, calculation procedure provides feedback signal, by controlling the power of flexible oven heated resistance wire 3, continuous heating; As actual temperature t _RealWhen being increased to the difference of target temperature T less than or equal to certain Δ t, computing machine sends the feedback signal of the power that reduces resistive heater 3, by controlling the performance number of flexible oven heated resistance wire 3, reduce the rate of heat addition, make resistive heater 3 slowly be heated to the target temperature T that sample needs; As sample actual temperature t _RealIn the target temperature T situation of wishing to reach higher than sample, and difference controls less than certain Δ t ' time the feedback signal that computing machine sends the power that reduces resistive heater 3, to reduce the rate of heat addition, resistive heater 3 is slowly heated, until target temperature T and described actual temperature t _RealDifference more than or equal to certain Δ t ' time, stopped heating.By this method, can the Quality control temperature reach the required target temperature T of user.

In actual use, described flexible thermostat is looped around around planchet, and preferably, flexible thermostat connects planchet by the button 8 that is flexible coupling, with convenient parcel planchet.Fig. 2 has provided the annexation of flexible thermostat and planchet, and in figure, 9 is planchet.Be looped around planchet 9 flexible thermostat on every side by carrying out heat exchange with planchet 9, with the control of final realization to planchet 9 center sample temperatures.By said apparatus provided by the invention, the temperature of Quality control is until reach the required target temperature T of user easily.

Proposed by the invention keep the constant device of sample temperature can realize sample in 25～100 ℃ of temperature ranges on arbitrary temperature holding temperature constant.

Above-described specific embodiment; purpose of the present invention, technical scheme and beneficial effect are further described; institute is understood that; the above is only specific embodiments of the invention; be not limited to the present invention; within the spirit and principles in the present invention all, any modification of making, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.

Claims

1. keep the constant device of sample temperature during a microexamination, it is characterized in that, described device comprises flexible insulating material 2, resistive heater 3, temperature thermocouple 4, data acquisition module 5, communication module 6, DA output module 7, wherein,

2. device according to claim 1, it is characterized in that, described device also comprises a junction button 8, described junction button 8 is used for connecting described device and planchet, so that described device is fixed on planchet, thus by with the final control that realizes planchet center sample temperature of the heat exchange of planchet.

3. device according to claim 2, is characterized in that, described junction button 8 is the button that is flexible coupling, make described device can be looped around with wrapping up planchet around.

4. device according to claim 1, is characterized in that, described DA output module 7 is controlled the rate of heat addition of described resistive heater 3 by the watt level of controlling described resistive heater 3.

5. device according to claim 2, is characterized in that, the actual temperature t of sample in described planchet _RealThe temperature t that records with described temperature thermocouple 4 _ThermostatThere is a corresponding relation.

6. device according to claim 1, is characterized in that, the temperature t that described control computing machine records according to described temperature thermocouple 4 _ThermostatAnd the predefined sample target temperature T that need to reach generates a feedback signal, controls the heating-up temperature of described device.

7. device according to claim 6, is characterized in that, described control computing machine generates a feedback signal and further comprises, the temperature t that records according to described temperature thermocouple 4 _ThermostatActual temperature t with sample _RealBetween the corresponding relation that exists, obtain the temperature t that records with described temperature thermocouple 4 _ThermostatThe actual temperature t of corresponding sample _Real, and according to described target temperature T and described actual temperature t _RealDifference generate described feedback signal.

8. device according to claim 7, is characterized in that, according to described target temperature T and described temperature t _RealDifference generate described feedback signal and further comprise:

As described actual temperature t _RealLower than target temperature T, and target temperature T and described actual temperature t _RealDifference during greater than Δ t, control computing machine and send the feedback signal that makes resistive heater 3 continuous heatings, as described actual temperature t _RealWhen being increased to difference with target temperature T less than or equal to Δ t, controlling computing machine and send the feedback signal that reduces the rate of heat addition, so that resistive heater 3 slowly is heated to target temperature T;

As described actual temperature t _RealHigher than target temperature T, and target temperature T and described actual temperature t _RealDifference less than Δ t ' time, control computing machine and send the feedback signal that reduces the rate of heat addition, so that resistive heater 3 slowly heats, until target temperature T and described actual temperature t _RealDifference more than or equal to Δ t ', then stopped heating.

9. according to claim 1 or 8 described devices, is characterized in that, described feedback signal is the feedback signal that increases/reduce the power of described resistive heater 3.

10. device according to claim 1, is characterized in that, described device can realize that sample arbitrary temperature in 25～100 ℃ of temperature ranges keeps temperature constant.