CN103135632B - Device for keeping temperature of samples constant while at time of microexamination - Google Patents
Device for keeping temperature of samples constant while at time of microexamination Download PDFInfo
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- CN103135632B CN103135632B CN201110386207.3A CN201110386207A CN103135632B CN 103135632 B CN103135632 B CN 103135632B CN 201110386207 A CN201110386207 A CN 201110386207A CN 103135632 B CN103135632 B CN 103135632B
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- temperature
- feedback signal
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- resistive heater
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Abstract
The invention discloses a device for keeping temperature of samples constant while at the time of microexamination. The device comprises flexible insulation materials 2, a resistive heater3, a temperature thermocouple 4, a data acquisition module 5, a communication module 6 and a DA output module 7. Electric resistance wire covered by the flexible insulation materials are used for heating, temperature signals of the flexible insulation materials are collected through a thermocouple sensor and are sent to a control computer to form a negative feedback, computer feedback signals are sued for keeping the temperature of the samples constant. While being used, the device encircles a sample vessel; the temperature of the samples in the centre of the sample vessel is controlled by exchanging heat with the sample vessel. The device is capable of keeping any temperature constant at the temperature range of 25 DEG C-100 DEG C. The device is connected with the sample vessel through a flexible connection buckle so as to conveniently cover the sample vessel.
Description
Technical field
The invention belongs to analytical and testing instrument field, while relating in particular to a kind of microexamination, maintain the constant device of sample temperature, specifically, the present invention is to adopting the coated resistance wire of flexible insulating material to heat, by regulating the power of resistive heater to maintain the temperature constant of sample in planchet.
Background technology
In chemical production and process of scientific research, the microcosmic optical morphology in the time of often need to observing sample different temperatures in real time, online by microscope.Although the temperature constant that traditional water-bath, electric jacket, constant temperature oven etc. can Quality controls, cannot realize by microscope and observe in real time, online sample.There is in the market the microscopical changing device that is exclusively used in that INSTEC company produces, can realize temperature the continuous variation of-78 ℃-250 ℃, but its complicated operation, apparatus expensive, common laboratory is held and is dared not accept.In addition, this changing device, in view of the design of himself, has a maximum deficiency: microscope is only to observe with long reach object lens, and the operating distance of general object lens is not enough to observe sample, in view of the costliness of object lens price, common laboratory is unworthy of or only joins one or two long reach object lens.Therefore, greatly limited being widely used of this changing device.Tokai Hit company has produced a kind of CO on the market
2incubating device, is used for biological sample to maintain 37 ℃, CO as cell etc.
2content remains on 5%, humidity 90%.This device is applicable to doing biological sample very much, but price is also very expensive, and complicated operation, also needs CO
2steel cylinder, floor area is large.Another problem of this device is: this thermostat conducts heat by air, conducts heat slow, is applicable to maintaining some temperature-resistant, is not suitable for doing alternating temperature experiment.For chemical example, often need to analyze be different temperatures time sample optical signature, and for cell sample, with respect to humidity and CO
2content, temperature is most important, as long as temperature maintains 37 ℃, the time-to-live of cell will be than greatly increasing at 25 ℃ of room temperatures.
Summary of the invention
In order to overcome above-mentioned defect, the present invention has designed a kind of constant device of sample temperature that simply maintains, and during microexamination, can control in real time, online temperature.
In bioanalysis test, cell is generally cultivated in confocal ware, and confocal ware also can be put chemical example certainly, and for examining under a microscope its optical signature, this is the planchet of indication of the present invention namely.While the object of the invention is to propose a kind of microexamination, maintain the constant device of sample temperature, it is characterized in that, described device comprises flexible insulating material 2, resistive heater 3, temperature thermocouple 4, data acquisition module 5, communication module 6, DA output module 7, wherein
Described resistive heater 3 is wrapped in described flexible insulating material 2 inside, for heating the temperature that improves described flexible insulating material 2;
Described temperature thermocouple 4 is placed in the center of described flexible insulating material 2, for measuring the temperature of described flexible insulating material 2, and produces a temperature signal;
Described data acquisition module 5 gathers described temperature signal and transfers to control computing machine by communication module 6;
Described DA output module 7 receives controls the feedback signal that computing machine sends over by communication module 6, and according to described feedback signal, controls the rate of heat addition of described resistive heater 3.
The device that utilizes the present invention to design can be realized and observe in real time, online the optical morphology of sample different temperatures on the instruments such as simple microscope, fluorescent microscope, Laser Scanning Confocal Microscope and Two Photon Fluorescence.As long as object lens can bear the temperature of 25-100 ℃, just can use any object lens, irrelevant with the operating distance of object lens.And this device is simple, cheap, contributes to the popularization in each laboratory.
Accompanying drawing explanation
Fig. 1 is the proposed by the invention structural representation and the fundamental diagram that maintain sample temperature constant device-flexible thermostat.
Fig. 2 is the flexible thermostat shown in Fig. 1 and the connection diagram of planchet.
Embodiment
For making the object, technical solutions and advantages of the present invention clearer, below in conjunction with specific embodiment, and with reference to accompanying drawing, the present invention is described in more detail.
The invention provides a kind of constant device of sample temperature that maintains, especially maintain the constant device of sample temperature during a kind of microexamination, Fig. 1 maintains structural representation and the fundamental diagram of sample temperature constant device-flexible thermostat described in being, in figure: 1 is whole flexible thermostat, and 2 is flexible insulating material, 3 is resistive heater, 4 is temperature thermocouple, and 5 is data acquisition module, and 6 is communication module, 7 is DA output module, and 8 is junction button.Flexible thermostat 1 shown in figure is mainly comprised of flexible insulating material 2, resistive heater 3, temperature thermocouple 4, data acquisition module 5, communication module 6, DA output module 7 and junction button 8.
Flexible insulating material 2 internal package resistive heaters 3 are used for heating raising temperature, temperature thermocouple 4 is housed to measure the temperature of flexible insulating material at flexible insulating material center, it is the temperature of flexible thermostat, and producing a temperature signal, data acquisition module 5 gathers described temperature signal and transfers to control computing machine by communication module 6; DA output module 7 receives controls the feedback signal that computing machine sends over by communication module 6, and according to described feedback signal, controls the rate of heat addition of described resistive heater 3.The effect of junction button 8 is for the flexible thermostat of fastening and planchet, so that flexible thermostat is fixed on planchet.
In fact, the actual temperature t of sample in planchet
realthe temperature t recording with flexible thermostat thermopair
thermostatdifferentiated, sample actual temperature t
realcan directly measure by thermometer, be the temperature t of flexible thermostat and we can control
thermostat.In certain temperature range, determine the flexible thermostat temperature temperature t that namely thermopair records
thermostatwith sample actual temperature t
realcorresponding relation, fit to a function.Like this, when sample will reach some temperature t
realtime, according to above-mentioned function, just can determine the required temperature t reaching of flexible thermostat
thermostat.
Control computing machine according to the program based on thermal conduction study design, the temperature that need to reach according to predefined actual sample is controlled the required heating-up temperature of flexible thermostat, and provide a feedback signal, this feedback signal transfers to DA output module 7 through communication module 6, thereby the watt level of utilizing DA output module 7 to control resistive heater is controlled the rate of heat addition of resistive heater 3.
Above-mentioned functions realizes by computer-controlled heating schedule, and detailed process is as follows: make sample reach the target temperature T of requirement of experiment, need to know target temperature T and sample actual temperature t at that time
realdifference decide the heating schedule of flexible thermostat.Actual temperature t at sample
realin the situation of the target temperature T reaching lower than hope, as target temperature T and by the thermocouple measuring temperature t of flexible thermostat
thermostatcorresponding sample actual temperature t
realdifference while being greater than certain Δ t, calculation procedure provides feedback signal, by controlling the power of flexible oven heated resistance wire 3, continuous heating; As actual temperature t
realwhen being increased to difference with target temperature T and being less than or equal to certain Δ t, computing machine sends the feedback signal of the power that reduces resistive heater 3, by controlling the performance number of flexible oven heated resistance wire 3, reduce the rate of heat addition, make resistive heater 3 slowly be heated to the target temperature T that sample needs; As sample actual temperature t
realin the target temperature T situation of wishing to reach higher than sample, and difference is less than certain Δ t ' time, control the feedback signal that computing machine sends the power that reduces resistive heater 3, to reduce the rate of heat addition, resistive heater 3 is slowly heated, until target temperature T and described actual temperature t
realdifference be more than or equal to certain Δ t ' time, stop heating.By this method, can Quality control temperature reach the required target temperature T of user.
In actual use, described flexible thermostat is looped around around planchet, and preferably, flexible thermostat connects planchet by the button 8 that is flexible coupling, to facilitate parcel planchet.Fig. 2 has provided the annexation of flexible thermostat and planchet, and in figure, 9 is planchet.Be looped around planchet 9 flexible thermostat around by carrying out heat exchange with planchet 9, finally to realize the control to planchet 9 center sample temperatures.By said apparatus provided by the invention, the temperature of Quality control is until reach the required target temperature T of user easily.
Proposed by the invention maintain the constant device of sample temperature can realize sample in 25～100 ℃ of temperature ranges in arbitrary temperature holding temperature constant.
Above-described specific embodiment; object of the present invention, technical scheme and beneficial effect are further described; institute is understood that; the foregoing is only specific embodiments of the invention; be not limited to the present invention; within the spirit and principles in the present invention all, any modification of making, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (9)
1. maintain the constant device of sample temperature during a microexamination, it is characterized in that, described device comprises flexible insulating material (2), resistive heater (3), temperature thermocouple (4), data acquisition module (5), communication module (6), DA output module (7), wherein
Described resistive heater (3) is wrapped in described flexible insulating material (2) inside, for heating the temperature that improves described flexible insulating material (2);
Described temperature thermocouple (4) is placed in the center of described flexible insulating material (2), for measuring the temperature of described flexible insulating material (2), and produces a temperature signal;
Described data acquisition module (5) gathers described temperature signal and transfers to control computing machine by communication module (6);
Described DA output module (7) receives controls the feedback signal that computing machine sends over by communication module (6), and according to described feedback signal, controls the rate of heat addition of described resistive heater (3);
The temperature t that described control computing machine records according to described temperature thermocouple (4)
thermostatand the target temperature T that predefined sample need to reach generates a feedback signal, control the heating-up temperature of described device.
2. device according to claim 1, it is characterized in that, described device also comprises a junction button (8), described junction button (8) is for connecting described device and planchet, so that described device is fixed on planchet, thereby finally realize the control to planchet center sample temperature by the heat exchange with planchet.
3. device according to claim 2, is characterized in that, described junction button (8) is the button that is flexible coupling, and described device can be wrapped up and be looped around planchet around.
4. device according to claim 1, is characterized in that, described DA output module (7) is controlled the rate of heat addition of described resistive heater (3) by controlling the watt level of described resistive heater (3).
5. device according to claim 2, is characterized in that, the actual temperature t of sample in described planchet
realthe temperature t recording with described temperature thermocouple (4)
thermostatthere is a corresponding relation.
6. device according to claim 1, is characterized in that, described control computing machine generates a feedback signal and further comprises, the temperature t recording according to described temperature thermocouple (4)
thermostatactual temperature t with sample
realbetween the corresponding relation that exists, obtain the temperature t recording with described temperature thermocouple (4)
thermostatthe actual temperature t of corresponding sample
real, and according to described target temperature T and described actual temperature t
realdifference generate described feedback signal.
7. device according to claim 6, is characterized in that, according to described target temperature T and described temperature t
realdifference generate described feedback signal and further comprise:
As described actual temperature t
reallower than target temperature T, and target temperature T and described actual temperature t
realdifference while being greater than Δ t, control computing machine and send the feedback signal that makes resistive heater (3) continuous heating, as described actual temperature t
realwhen being increased to difference with target temperature T and being less than or equal to Δ t, control computing machine and send the feedback signal that reduces the rate of heat addition, so that resistive heater (3) is slowly heated to target temperature T;
As described actual temperature t
realhigher than target temperature T, and target temperature T and described actual temperature t
realdifference while being less than Δ t ˊ, control computing machine and send the feedback signal that reduces the rate of heat addition, so that resistive heater (3) slowly heats, until target temperature T and described actual temperature t
realdifference be more than or equal to Δ t ˊ, then stop heating.
8. according to the device described in claim 1 or 7, it is characterized in that, described feedback signal is for increasing/reduce the feedback signal of the power of described resistive heater (3).
9. device according to claim 1, is characterized in that, described device can be realized sample arbitrary temperature in 25～100 ℃ of temperature ranges and keep temperature constant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110386207.3A CN103135632B (en) | 2011-11-29 | 2011-11-29 | Device for keeping temperature of samples constant while at time of microexamination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110386207.3A CN103135632B (en) | 2011-11-29 | 2011-11-29 | Device for keeping temperature of samples constant while at time of microexamination |
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| Publication Number | Publication Date |
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| CN103135632A CN103135632A (en) | 2013-06-05 |
| CN103135632B true CN103135632B (en) | 2014-11-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110386207.3A Expired - Fee Related CN103135632B (en) | 2011-11-29 | 2011-11-29 | Device for keeping temperature of samples constant while at time of microexamination |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110118618A (en) * | 2018-02-05 | 2019-08-13 | 浙江智柔科技有限公司 | Thermostatic equipment |
| CN114860003A (en) * | 2022-05-12 | 2022-08-05 | 中国科学院苏州生物医学工程技术研究所 | PCR thermal cycle system control method, device, equipment and storage medium |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2283884Y (en) * | 1996-12-27 | 1998-06-10 | 江苏省畜牧兽医总站 | Microscope electronic thermostat |
| CN2927146Y (en) * | 2006-07-17 | 2007-07-25 | 王敏康 | Multi-purpose temperature-adjusting heating platform |
| CN101187816A (en) * | 2007-11-28 | 2008-05-28 | 华中科技大学 | Precision temperature controller |
| CN101655702A (en) * | 2009-09-10 | 2010-02-24 | 浙江大学 | Remote monitoring device of polymerase chain reaction (PCR) instrument |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4849961B2 (en) * | 2006-06-05 | 2012-01-11 | エスアイアイ・ナノテクノロジー株式会社 | Thermal analyzer |
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2011
- 2011-11-29 CN CN201110386207.3A patent/CN103135632B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2283884Y (en) * | 1996-12-27 | 1998-06-10 | 江苏省畜牧兽医总站 | Microscope electronic thermostat |
| CN2927146Y (en) * | 2006-07-17 | 2007-07-25 | 王敏康 | Multi-purpose temperature-adjusting heating platform |
| CN101187816A (en) * | 2007-11-28 | 2008-05-28 | 华中科技大学 | Precision temperature controller |
| CN101655702A (en) * | 2009-09-10 | 2010-02-24 | 浙江大学 | Remote monitoring device of polymerase chain reaction (PCR) instrument |
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| CN103135632A (en) | 2013-06-05 |
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