CN203007280U - Cell culturing bioreactor - Google Patents
Cell culturing bioreactor Download PDFInfo
- Publication number
- CN203007280U CN203007280U CN 201320020769 CN201320020769U CN203007280U CN 203007280 U CN203007280 U CN 203007280U CN 201320020769 CN201320020769 CN 201320020769 CN 201320020769 U CN201320020769 U CN 201320020769U CN 203007280 U CN203007280 U CN 203007280U
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- China
- Prior art keywords
- tank body
- breather
- air
- cell culture
- gas diffuser
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Abstract
The utility model discloses a cell culturing bioreactor. The cell culturing bioreactor comprises a tank body (5), an air breather and a stirrer (3). The stirrer (3) is arranged on the bottom of the tank body (5). The air breather comprises an air inlet (1), a breather pipe (6) and a gas diffuser (2), wherein the air inlet (1), the breather pipe (6) and the gas diffuser (2) are connected in sequence. The air inlet (1) is arranged on the upper end face of the tank body (5). The gas diffuser (2) is located on the lower portion of the tank body (5). The stirrer (3) is installed below the gas diffuser (2). A valve (4) is installed on the bottom of the tank body (5). Airflow can directly enter cell culturing fluid, and air bubbles can be reduced due to the fact that an air outlet of the air breather is formed in the bottom of the tank body. Due to the fact that the gas diffuser is installed on the air breather, the gas diffuser is made of a stainless steel filtering element screen, and therefore gas can be divided into a great deal of fine airflow to fuse into the liquid after the gas passes though the filtering element screen. The rate of producing the air bubbles can be greatly reduced.
Description
Technical field
The utility model relates to a kind of cell culture apparatus, is specifically related to a kind of biological reactor for cell culture.
Background technology
Cell culture technology also is the cell clone technology, and the regular noun in biology is cell culture technology.No matter for whole biotechnology, or the biological cloning technology of one of them, cell cultures is all a requisite process, cell cultures itself is exactly the extensive clone of cell.Cell cultures, both comprised the cultivation of microorganism cells, cell culture technology can be by a cell through a large amount of many cells that become simple unicellular or few differentiation of cultivating, and this is the requisite link of clone technology, and cell cultures itself is exactly the clone of cell.Obtain a large amount of cells or its meta-bolites by cell cultures.Because biological product is all to get from cell, so can say that cell culture technology is most crucial in biotechnology, most basic technology.In the prior art, ventilation is generally directly and ventilates in tank body by pipeline in the bioreactor, and this method easily produces bubble, and bubble breaks in cell cultures liquid and can cause cell injury even dead.
The utility model content
The technical problem that (one) will solve
The technical problems to be solved in the utility model is to provide a kind of biological reactor for cell culture, to solve the problem that produces bubble in venting process.
(2) technical scheme
for solving the problems of the technologies described above, the technical scheme that the utility model adopts is: a kind of biological reactor for cell culture, comprise tank body (5), breather, agitator (3), described agitator (3) is arranged at bottom described tank body (5), described breather comprises the inlet mouth (1) that connects successively, ventpipe (6) and gaseous diffuser (2), described inlet mouth (1) is arranged at described tank body (5) upper surface, described gaseous diffuser (2) is positioned at the bottom of tank body (5), described gaseous diffuser (2) below is provided with agitator (3), described tank body (5) bottom also is provided with valve (4).
Described gaseous diffuser (2) is made by stainless steel filter core net.
The outer thermal insulation layer (7) that is provided with of described tank body (5).
Also comprise an automatic control device, described automatic control device comprises housing (10) and stretches into the inductor block (8) of described tank body (5), described housing (10) is connected with the inlet mouth (1) of described breather by pipe connecting (9), described inductor block (8) is monitored the oxygen level of the reaction solution in described tank body (5), and described housing (10) is regulated gas pushing quantity automatically according to the signal of described inductor block (8) feedback.
(3) beneficial effect
The utility model is compared to prior art, has following beneficial effect: in the tank base position, make air-flow directly pass in cell culture fluid due to the air outlet of breather, reduce Bubble formation; Because breather is provided with gaseous diffuser, its gaseous diffuser is made by stainless steel filter core net, makes gas incorporate in liquid through being divided into many tiny air-flows after the filter core net, greatly reduces the probability that produces bubble.
Description of drawings
Fig. 1 is structural representation of the present utility model.
Embodiment
Below in conjunction with drawings and Examples, embodiment of the present utility model is described in further detail.Following examples are used for explanation the utility model, but are not used for limiting scope of the present utility model.
A kind of biological reactor for cell culture as shown in Figure 1, comprise tank body 5, breather, agitator 3, described agitator 3 is arranged at described tank body 5 bottoms, described breather comprises inlet mouth 1, ventpipe 6 and the gaseous diffuser 2 that connects successively, described inlet mouth 1 is arranged at described tank body 5 upper surfaces, described gaseous diffuser 2 is positioned at the bottom of tank body 5, and described gaseous diffuser 2 belows are provided with agitator 3, and described tank body 5 bottoms also are provided with valve 4.Described gaseous diffuser 2 is made by stainless steel filter core net.The outer thermal insulation layer 7 that is provided with of described tank body 5.This biological reactor for cell culture also comprises an automatic control device, described automatic control device comprises housing 10 and stretches into the inductor block 8 of described tank body 5, described housing 10 is connected with the inlet mouth 1 of described breather by pipe connecting 9, the oxygen level of the reaction solution in the described inductor block 8 described tank bodies 5 of monitoring, described housing 10 is regulated gas pushing quantity automatically according to the signal of described inductor block 8 feedbacks.
The utility model in the tank base position, makes air-flow directly pass in cell culture fluid due to the air outlet of breather, reduces Bubble formation; Because breather is provided with gaseous diffuser, its gaseous diffuser is made by stainless steel filter core net, makes gas incorporate in liquid through being divided into many tiny air-flows after the filter core net, greatly reduces the probability that produces bubble.
The above is only preferred implementation of the present utility model; should be understood that; for those skilled in the art; under the prerequisite that does not break away from the utility model know-why; can also make some improvements and modifications, these improvements and modifications also should be considered as protection domain of the present utility model.
Claims (4)
1. biological reactor for cell culture, it is characterized in that: comprise tank body (5), breather, agitator (3), described agitator (3) is arranged at bottom described tank body (5), described breather comprises inlet mouth (1), ventpipe (6) and the gaseous diffuser (2) that connects successively, described inlet mouth (1) is arranged at described tank body (5) upper surface, described gaseous diffuser (2) is positioned at the bottom of tank body (5), described gaseous diffuser (2) below is provided with agitator (3), and described tank body (5) bottom also is provided with valve (4).
2. biological reactor for cell culture according to claim 1, it is characterized in that: described gaseous diffuser (2) is made by stainless steel filter core net.
3. biological reactor for cell culture according to claim 2, is characterized in that: the outer thermal insulation layer (7) that is provided with of described tank body (5).
4. biological reactor for cell culture according to claim 3, it is characterized in that: also comprise an automatic control device, described automatic control device comprises housing (10) and stretches into the inductor block (8) of described tank body (5), described housing (10) is connected with the inlet mouth (1) of described breather by pipe connecting (9), described inductor block (8) is monitored the oxygen level of the reaction solution in described tank body (5), and described housing (10) is regulated gas pushing quantity automatically according to the signal of described inductor block (8) feedback.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320020769 CN203007280U (en) | 2013-01-15 | 2013-01-15 | Cell culturing bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320020769 CN203007280U (en) | 2013-01-15 | 2013-01-15 | Cell culturing bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203007280U true CN203007280U (en) | 2013-06-19 |
Family
ID=48598893
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320020769 Expired - Fee Related CN203007280U (en) | 2013-01-15 | 2013-01-15 | Cell culturing bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203007280U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113564049A (en) * | 2021-08-31 | 2021-10-29 | 南京海创佳生物科技有限公司 | Bioreactor |
-
2013
- 2013-01-15 CN CN 201320020769 patent/CN203007280U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113564049A (en) * | 2021-08-31 | 2021-10-29 | 南京海创佳生物科技有限公司 | Bioreactor |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130619 Termination date: 20150115 |
|
| EXPY | Termination of patent right or utility model |