CN202699644U - Recovery filtering device for blood serum or cell culture supernatant - Google Patents
Recovery filtering device for blood serum or cell culture supernatant Download PDFInfo
- Publication number
- CN202699644U CN202699644U CN201220175741XU CN201220175741U CN202699644U CN 202699644 U CN202699644 U CN 202699644U CN 201220175741X U CN201220175741X U CN 201220175741XU CN 201220175741 U CN201220175741 U CN 201220175741U CN 202699644 U CN202699644 U CN 202699644U
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- supernatant
- serum
- sleeve pipe
- cells
- centrifuge tube
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Abstract
The utility model relates to a recovery filtering device for blood serum or cell culture supernatant. The recovery filtering device for the blood serum or the cell culture supernatant is characterized by comprising a vacuum suction device (1) and a centrifuge tube (2). The vacuum suction device (1) comprises a casing tube (1.1) and a piston (1.2), wherein the piston (1.2) is arranged inside the casing tube (1.1), the bottom of the casing tube (1.1) is provided with a suction tube connector (1.4), the bottom of the suction tube connector (1.4) is connected with a suction tube (1.5), and the bottom of the casing tube (1.1) is in thread fit with the top of the centrifuge tube (2). According to the recovery filtering device for the blood serum or the cell culture supernatant, the suction device and a container are combined into a whole, the possibility of the contact between blood and the air is effectively reduced, and the possibility of bacterial pollution is reduced as well.
Description
Technical field
This utility model relates to the recovery defecator of a kind of serum or cells and supernatant, is used for osteoarthritis treatment.
Background technology
Human peripheral blood can produce the serum that contains the high concentration anti-inflammatory factors external after inducing, this antiinflammatory serum is recycled in patient's the articular cavity, can stop further developing and significantly improving symptom of the osteoarthritis state of an illness.
This process relates to syringe and extracts patient's peripheral blood; Peripheral blood is injected the container with induced material; Blood in the container makes it produce anti-inflammatory factors in external evoked cultivation; Adopt centrifugal method that serum is separated with hemocyte; Draw the serum part and installed to juxtaposition cryopreservation in the preservation pipe in rear minute.
Yet when injecting the serum keeping pipe after the serum of cultivating in container with aspirator extracts, blood can come in contact with air, causes easily germ contamination, thereby has affected the quality of antiinflammatory serum.
Summary of the invention
The purpose of this utility model structurally overcomes above-mentioned deficiency, providing a kind of is integral aspirator and container combination, the chance that contacts of blood and air be can effectively reduce, the serum of probability of germ contamination or the recovery defecator of cells and supernatant reduced.
The purpose of this utility model is achieved in that
The recovery defecator of this utility model serum or cells and supernatant, comprise vacuum suction apparatus and centrifuge tube, described vacuum suction apparatus comprises sleeve pipe and piston, described piston is located in the sleeve pipe, the bottom of described sleeve pipe is provided with suction attachment, the bottom of described suction attachment is connected to suction pipe, and described sleeve bottom cooperates with the screw top of centrifuge tube.
The bottom of described sleeve pipe is protruded and is provided with female thread, and the top end opening of described centrifuge tube also is provided with external screw thread, and female thread cooperates with external screw thread.
Described sleeve pipe lower sides has filtering holes, is respectively equipped with the air filtration filler in the filtering holes, and the aperture of air filtration filler is less than 0.22 μ m.
The outer race of described suction attachment has germ tight filter.
The bottom of described suction pipe is bent upwards into hook-shaped.
The bottom of described centrifuge tube is placed with some glass beads.
The recovery defecator of this utility model serum or cells and supernatant has the following advantages:
The recovery defecator of this serum or cells and supernatant changes vacuum suction apparatus and centrifuge tube into removable structure, the serum that the In vitro culture that vacuum suction apparatus can be extracted out from centrifuge tube goes out to contain anti-inflammatory factors is delivered directly in the serum keeping pipe, blood and contact with air have effectively been reduced, reduce the possibility of germ contamination, thereby guaranteed the quality of serum.
Description of drawings
Fig. 1 is the structural representation of the recovery defecator of this utility model serum or cells and supernatant.
Fig. 2 is the structural representation of vacuum suction apparatus among Fig. 1.
Fig. 3 is the structural representation of centrifuge tube among Fig. 1.
Among the figure: vacuum suction apparatus 1, sleeve pipe 1.1, piston 1.2, female thread 1.3, suction attachment 1.4, suction pipe 1.5, germ tight filter 1.6, filtering holes 1.7;
The specific embodiment
Referring to Fig. 1 to Fig. 3, this utility model relates to the recovery defecator of a kind of serum or cells and supernatant, and it comprises vacuum suction apparatus 1 and centrifuge tube 2;
Described vacuum suction apparatus 1 comprises sleeve pipe 1.1 and piston 1.2, described piston 1.2 is located in the sleeve pipe 1.1, and the upper end is exposed to sleeve pipe 1.1, can in sleeve pipe 1.1, move up and down, the bottom protrusion of described sleeve pipe 1.1 also is provided with female thread 1.3, the bottom of described sleeve pipe 1.1 is provided with suction attachment 1.4, the bottom of described suction attachment 1.4 is connected to suction pipe 1.5, the bottom of described suction pipe 1.5 is bent upwards into hook-shaped, the outer race of described suction attachment 1.4 has germ tight filter 1.6, this germ tight filter 1.6 can be removed hemocyte that may be residual in the serum and the antibacterial that may pollute, described sleeve pipe 1.1 bottoms have filtering holes 1.7 near on the sidewall at female thread 1.3 places, when this filtering holes 1.7 can be avoided vacuum suction apparatus 1 suction in sleeve pipe 1.1 interior formation negative pressure, be provided with the air filtration filler in the described filtering holes 1.7, the aperture of air filtration filler is passed through to allow air free, and is prevented that antibacterial from passing through less than 0.22 μ m.
The top end opening of described centrifuge tube 2 is provided with external screw thread 2.1, and the bottom of centrifuge tube 2 is placed with some glass beads 2.2, and described glass bead 2.2 can induce peripheral blood to produce the anti-inflammatory factors of high concentration.
Described sleeve pipe 1.1 bottoms can cooperate with the external screw thread 2.1 of centrifuge tube 2 by female thread 1.3.
The recovery defecator of this serum or cells and supernatant in use, by suction pipe 1.5 serum is taken out, owing to the terminal crooked of suction pipe 1.5 upwards arranges, suction pipe 1.5 just in time is positioned at the top of hemocyte face, can be to the interference of cell generation of interfaces and with the hemocyte sucking-off.After sucking, again serum is injected the serum keeping pipe 3 rearmounted low temperature of sealing (80 C °) and deposit.
Claims (6)
1. the recovery defecator of a serum or cells and supernatant, it is characterized in that: it comprises vacuum suction apparatus (1) and centrifuge tube (2), described vacuum suction apparatus (1) comprises sleeve pipe (1.1) and piston (1.2), described piston (1.2) is located in the sleeve pipe (1.1), the bottom of described sleeve pipe (1.1) is provided with suction attachment (1.4), the bottom of described suction attachment (1.4) is connected to suction pipe (1.5), and described sleeve pipe (1.1) bottom cooperates with the screw top of centrifuge tube (2).
2. the recovery defecator of a kind of serum according to claim 1 or cells and supernatant, it is characterized in that: the bottom protrusion of described sleeve pipe (1.1) also is provided with female thread (1.3), the top end opening of described centrifuge tube (2) also is provided with external screw thread (2.1), and female thread (1.3) cooperates with external screw thread (2.1).
3. the recovery defecator of a kind of serum according to claim 1 or cells and supernatant, it is characterized in that: described sleeve pipe (1.1) lower sides has filtering holes (1.7), filtering holes is respectively equipped with the air filtration filler in (3.7), and the aperture of air filtration filler is less than 0.22 μ m.
4. the recovery defecator of a kind of serum according to claim 1 or cells and supernatant, it is characterized in that: the outer race of described suction attachment (1.4) has germ tight filter (1.6).
5. the recovery defecator of a kind of serum according to claim 1 or cells and supernatant, it is characterized in that: the bottom of described suction pipe (1.5) is bent upwards into hook-shaped.
6. the recovery defecator of a kind of serum according to claim 1 or cells and supernatant, it is characterized in that: the bottom of described centrifuge tube (2) is placed with some glass beads (2.2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201220175741XU CN202699644U (en) | 2012-04-24 | 2012-04-24 | Recovery filtering device for blood serum or cell culture supernatant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201220175741XU CN202699644U (en) | 2012-04-24 | 2012-04-24 | Recovery filtering device for blood serum or cell culture supernatant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202699644U true CN202699644U (en) | 2013-01-30 |
Family
ID=47579992
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201220175741XU Expired - Fee Related CN202699644U (en) | 2012-04-24 | 2012-04-24 | Recovery filtering device for blood serum or cell culture supernatant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202699644U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018077120A1 (en) * | 2016-10-24 | 2018-05-03 | 深圳市孔雀生物科技有限公司 | Combination-type centrifugal device |
-
2012
- 2012-04-24 CN CN201220175741XU patent/CN202699644U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018077120A1 (en) * | 2016-10-24 | 2018-05-03 | 深圳市孔雀生物科技有限公司 | Combination-type centrifugal device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130130 Termination date: 20130424 |