CN202649096U

CN202649096U - Cuvette device

Info

Publication number: CN202649096U
Application number: CN 201220228103
Authority: CN
Inventors: 粟晖; 姚志湘; 葛军; 刘柳
Original assignee: Guangxi University of Science and Technology
Current assignee: Guangxi University of Science and Technology
Priority date: 2012-05-21
Filing date: 2012-05-21
Publication date: 2013-01-02
Anticipated expiration: 2022-05-21

Abstract

The utility model provides a cuvette device and relates to a device used in spectroscopic analysis. The device comprises a cuvette bracket and a cuvette, wherein the cuvette comprises a conventional cuvette capable of realizing common ultraviolet visible spectrophotometry measurement function and a flowing cuvette capable of realizing liquid chromatogram multi-channel photometric detection function; the flowing cuvette comprises a cuvette body, a flowing cuvette channel is arranged in the cuvette body, and the diameter of the flowing cuvette channel is gradually increased along the flowing direction; the top end of the cuvette body is provided with a feeding pipe and a downward bent discharging pipe; the feeding pipe is connected with a liquid chromatogram inflow pipe through a pipeline joint; the cuvette bracket comprises a base and a cylindrical bracket wall, wherein the cylindrical bracket wall is provided with optical fiber alignment in/out joints; and the optical fiber alignment in/out joints are respectively connected with fibers positioned on two sides of the cuvette device through a fiber coupling joint. According to the cuvette device, a multifunctional spectrophotometer matched with the cuvette device can be used for multiple purposes, and the structure of the cuvette device is reasonable and reliable, so that the signal to noise ratio of the instrument can be reduced.

Description

Cuvette device

Technical field

The utility model relates to the device that uses in a kind of spectral analysis, particularly a kind of can with the matching used cuvette device of multi-usage spectrophotometer as liquid chromatography multichannel array detecting device and common transmission measurement.

Background technology

UV-visible detector is the maximum detecting device of configuration in the liquid chromatography, along with technical development and application level promote, can measure simultaneously the use of the hyperchannel UV-visible detector of multi-wavelength response and also day by day popularize.Multichannel detector can detect the spectrum change that chromatogram flows out process in liquid chromatography, realize the functions such as qualitative, quantitative, peak purity check, finger-print expression by spectrum.The liquid chromatography photometric detector can be realized the spectrophotometer of continuous coverage from the principle exactly, but because the testing goal difference, spectrophotometer and liquid chromatogram detector all are to create two kinds of instruments according to not isostructure, finish different analysis task.

Spectrophotometer is the conventional analysis instrument, and along with the laboratory equipment the level rise, the liquid chromatography photometric detector also becomes the conventional analysis instrument gradually, and in the laboratory, spectrophotometric usage frequency is usually greater than the liquid chromatography photometric detector.Spectrophotometer and liquid chromatography photometric detector are as the instrument of same principle, because structural differences has caused the function repeated configuration.Multi-wavelength scanning type photometer and hyperchannel chromatogram photometric detector, because with better function, configuration and use also day by day increase, but instrument cost is higher at present, has affected popularization.

Summary of the invention

The technical problems to be solved in the utility model is: the matching used cuvette device of multi-usage spectrophotometer a kind of and as liquid chromatography multichannel array detecting device and common transmission measurement is provided.

The technical scheme that solves the problems of the technologies described above is: a kind of cuvette device, comprise the cuvette support and can realize the cuvette that the visible spectrophotometry of general ultraviolet, liquid chromatography hyperchannel luminosity detect, trace flows the colorimetric measurement function, this cuvette is installed on the cuvette support.

Further technical scheme of the present utility model is: described cuvette comprises the conventional cuvette that can realize the visible spectrophotometry function of general ultraviolet and can realize the floating cuvette that liquid chromatography hyperchannel luminosity detects, trace flows the colorimetric measurement function.

Further technical scheme of the present utility model is: described floating cuvette comprises airtight cuvette body, is provided with in the cuvette body to flow than the chrominance channel, and this mobile diameter streamwise than the chrominance channel increases gradually; The top of cuvette body is respectively equipped with and flows the sample feeding pipe that is connected than the chrominance channel and reclinately go out the sample pipe, goes out the caliber of sample pipe greater than the caliber of sample feeding pipe, and sample feeding pipe is connected with the liquid chromatography inflow pipe by pipe joint.

Sample feeding pipe in the described floating cuvette, go out the sample pipe and flow all to adopt the fine pore of diameter≤2mm than the caliber of chrominance channel, sample feeding pipe, go out the sample pipe and flow more level and smooth than the inside of chrominance channel and the junction is seamless.

Described pipe joint comprises joint body, this joint body is fixed on the porch of sample feeding pipe, joint body is provided with the connecting hole that diameter increases gradually between liquid chromatography inflow pipe and sample feeding pipe, joint body also is provided with stepped hole in the upper end, and be provided with external thread at the outer wall of stepped hole, clamp ring for clamping liquid chromatography inflow pipe is installed on the described stepped hole, and is equipped with for the pipe joint nut that compresses clamp ring and match with the external thread of joint body at the clamp ring top.

Described joint body adopts Teflon or stainless steel material to make.

Further technical scheme of the present utility model is: described cuvette support comprises base, is fixed on the cylinder-shaped bracket wall on the base, be placed with described cuvette in the cylinder-shaped bracket wall, described cylinder-shaped bracket wall is equipped with fiber optic collimator access joint at the light light incident side, at the light emitting side fiber optic collimator is installed and picks out joint, this fiber optic collimator access joint, fiber optic collimator pick out joint and are connected with the optical fiber that is positioned at cuvette device light light incident side and emitting side by the coupling fiber joint respectively.

Described fiber optic collimator access joint comprises internal thread sleeve pipe, male-pipe A and male-pipe B, the endoporus middle part of described internal thread sleeve pipe is equipped with collimation lens set, male-pipe A and male-pipe B are installed in respectively the both sides of collimation lens set, male-pipe A is connected with the optical fiber that is positioned at cuvette device light light incident side by the coupling fiber joint, and male-pipe B is connected with the cylinder-shaped bracket wall; Described fiber optic collimator picks out joint and comprises internal thread sleeve pipe, male-pipe C and male-pipe D, the endoporus middle part of described internal thread sleeve pipe is equipped with collimation lens B, male-pipe C and male-pipe D are installed in respectively the both sides of collimation lens, male-pipe C is connected with the optical fiber that is positioned at cuvette device light emitting side by the coupling fiber joint, and male-pipe D is connected with the cylinder-shaped bracket wall.

Described coupling fiber joint comprises ferrule and the flexible optical fiber jacket of fibre-optical splice nut, fiber orientation snap ring, rigidity, described ferrule, optical fiber jacket are coated on respectively outside the optical fiber, ferrule is inserted in the through hole of male-pipe A or male-pipe C, and spacing by the fiber orientation snap ring, described fibre-optical splice nut is installed in outside male-pipe A or the male-pipe C and with the fiber orientation snap ring and compresses.

Because adopt technique scheme, the cuvette device of the utility model compared with prior art has following beneficial effect:

1, can make with it supporting multi-usage spectrophotometer realize a tractor serves several purposes:

Because the utility model comprises the cuvette support and can realize the cuvette that the visible spectrophotometry of general ultraviolet, liquid chromatography hyperchannel luminosity detect, trace flows the colorimetric measurement function, namely be when described cuvette adopts conventional cuvette, can realize the function of the visible spectrophotometry of general ultraviolet, can make common spectrophotometer and use; When described cuvette adopts floating cuvette, flow cell in the alternative traditional hyperchannel liquid chromatography photometric detector of this floating cuvette, can realize the function that liquid chromatography hyperchannel luminosity detects, can be used as liquid chromatography hyperchannel luminosity detecting device and use; When described cuvette adopts floating cuvette and microsyringe sample introduction, can realize the function of the mobile colorimetric measurement of trace.Therefore, the utility model can make with it supporting multi-usage spectrophotometer realize the purpose of a tractor serves several purposes, thereby but the lowering apparatus deployment cost, improve the instrument utilization ratio, strengthen the instrument integration degree, improve the rationality of middle-size and small-size Laboratory Instruments resource distribution, promote popularizing of multi-channel detection.

2, rational in infrastructure reliable:

Because the characteristic of mobile phase of high performance liquid chromatography is continuously, trace, be plunger flow, the floating cuvette that the utility model adopts substitutes the multi-passage circulating pond and has fully taken into account these characteristics.The floating cuvette that the utility model adopts comprises airtight cuvette body, being provided with in the cuvette body flows compares the chrominance channel, should increase gradually by mobile diameter streamwise than the chrominance channel, in order to keep small pressure reduction, suppress mobile back-mixing and diffusion, the while caliber increases gradually can also offset part diffusion length, is conducive to keep separating effect; The top of cuvette body is respectively equipped with and flows the sample feeding pipe that is connected than the chrominance channel and reclinately go out the sample pipe, go out the caliber of sample pipe greater than the caliber of sample feeding pipe, suppress mobile back-mixing to form micro-pressure reduction, sample feeding pipe is connected with the liquid chromatography inflow pipe by pipe joint; In addition, all calibers in the floating cuvette all adopt fine pore, can guarantee the requirement of micro-flow; Sample feeding pipe, go out the sample pipe and flow more level and smooth than the inside of chrominance channel and the junction is seamless, can prevent that mobile phase is resident or form eddy current.Therefore, structure of the present utility model is very rationally reliable.

But the signal to noise ratio (S/N ratio) of 3 lowering apparatus:

Because the signal to noise ratio (S/N ratio) of the luminous flux direct relation instrument that absorbance is measured, the utility model proposes non-parallel light path transmission design and improve luminous flux, namely be that incident light enters from the mobile outflow direction than the chrominance channel of floating cuvette, in the fiber optic collimator access joint of cuvette support, adopt the combination of collimation lens set, focus is adjusted near mobile colorimetric feeder connection, can utilize so the larger area of colorimetric channel outlet that flows, import larger luminous flux; Be coupled into optical fiber through the collimated that fiber optic collimator picks out in the joint again by the light of dispersing after the focus, import rearmounted light splitting and array electrooptical device.Therefore, but the utility model by improving the also signal to noise ratio (S/N ratio) of lowering apparatus of luminous flux.

Below, in conjunction with the accompanying drawings and embodiments the technical characterictic of the cuvette device of the utility model is further described.

Description of drawings

Fig. 1～Fig. 2: the structural representation of floating cuvette,

Fig. 1: the main cut-open view, Fig. 2 looked: stereographic map.

Fig. 3: the structural representation of the utility model embodiment one described pipe joint,

Fig. 4: the structural representation of the utility model embodiment one described cuvette support,

Fig. 5: flow than the logical schematic diagram of light of chrominance channel,

Fig. 6: the structural representation of the utility model embodiment one described fiber optic collimator access joint,

Fig. 7: the utility model embodiment one described fiber optic collimator picks out the structural representation of joint,

Fig. 8: the structural representation of the utility model embodiment one described coupling fiber joint.

In above-mentioned accompanying drawing, the label of each element is as follows:

1-cuvette support, the 11-base, 12-cylinder-shaped bracket wall, 13-fiber optic collimator access joint,

131-internal thread sleeve pipe, 132-male-pipe A, 133-male-pipe B, the 134-collimation lens set,

The 14-fiber optic collimator picks out joint, 141-internal thread sleeve pipe, and 142-male-pipe C, 143-male-pipe D,

144-collimation lens B, 15-coupling fiber joint, 151-fibre-optical splice nut, 152-fiber orientation snap ring,

The 153-ferrule, the 154-optical fiber jacket, the 2-floating cuvette, 21-cuvette body,

22-flows than the chrominance channel, and 23-sample feeding pipe, 24-go out the sample pipe, the 25-pipe joint,

The 251-joint body, the 252-connecting hole, the 253-stepped hole, the 254-clamp ring,

255-pipe joint nut, 26-liquid chromatography inflow pipe,

The flow direction of arrow A-mobile phase, arrow B-light transmission direction, arrow C-cuvette placement direction,

O-colorimetric channel outlet the cross section of flowing, the I-colorimetric feeder connection cross section of flowing.

Embodiment

Embodiment one:

A kind of cuvette device, comprise cuvette support 1 and be installed in conventional cuvette and floating cuvette 2 on the cuvette support 1, by the switching of conventional cuvette and floating cuvette 2, can realize that the visible spectrophotometry of general ultraviolet, liquid chromatography hyperchannel luminosity detect, trace flows the functions such as colorimetric measurement function.

Described conventional cuvette is known structure, no longer its structure is given unnecessary details in detail here; Described floating cuvette 2 is used to form the ratio chrominance channel of tested mobile phase, such as Fig. 1～shown in Figure 2, this floating cuvette 2 comprises airtight cuvette body 21, be provided with in the cuvette body 21 and flow than chrominance channel 22, mobile diameter streamwise than chrominance channel 22 increases gradually, to keep small pressure reduction, suppresses back-mixing and the diffusion of flowing, the while caliber increases gradually can also offset part diffusion length, is conducive to keep separating effect; The top of cuvette body 21 is respectively equipped with and flows the sample feeding pipe 23 that is connected than chrominance channel 22 and reclinately go out sample pipe 24, go out the caliber of sample pipe 24 greater than the caliber of sample feeding pipe 23, sample feeding pipe 23 is connected with liquid chromatography inflow pipe 26 by pipe joint 25, go out sample pipe 24 and be bent downwardly the mobile phase that to avoid deriving and form step in the exit, cause liquid residue and backflow; Sample feeding pipe 23 in the described floating cuvette 2, go out sample pipe 24 and flow all to adopt the fine pore of diameter≤2mm than the caliber of chrominance channel 22, sample feeding pipe 23, go out sample pipe 24 and flow more level and smooth than the inside of chrominance channel 22 and the junction is seamless, to prevent that mobile phase is resident or to form eddy current; Described pipe joint 25 comprises joint body 251, and this joint body 251 adopts the Teflon material to make, and its material surface tension force makes mobile phase be not easy to adhere at wall greater than mobile phase; Joint body 251 is fixed on the porch of sample feeding pipe 23, joint body 251 is provided with the connecting hole 252 that diameter increases gradually between liquid chromatography inflow pipe 26 and sample feeding pipe 23, joint body 251 also is provided with stepped hole 253 in the upper end, and be provided with external thread at the outer wall of stepped hole 253, clamp ring 254 for clamping liquid chromatography inflow pipe 26 is installed on the described stepped hole, and is equipped with for the pipe joint nut 255(that compresses clamp ring 254 and match with the external thread of joint body referring to Fig. 3 at clamp ring 254 tops).

Described cuvette support 1 comprises base 11, be fixed on cylinder-shaped bracket wall 12(on the base 11 referring to Fig. 4), cylinder-shaped bracket wall 12 is placed with described conventional cuvette or floating cuvette 2, described cylinder-shaped bracket wall 12 is equipped with fiber optic collimator access joint 13 at the light light incident side, at the light emitting side fiber optic collimator is installed and picks out joint 14, this fiber optic collimator access joint 13, fiber optic collimator pick out joint 14 and are connected with the optical fiber that is positioned at cuvette device 4 light light incident sides and emitting side by coupling fiber joint 15 respectively.

Described fiber optic collimator access joint 13 comprises that internal thread sleeve pipe 131, male-pipe A132 and male-pipe B133(are referring to Fig. 6), the endoporus middle part of described internal thread sleeve pipe 131 is equipped with collimation lens set 134, this collimation lens set 134 comprises two collimation lenses, male-pipe A132 and male-pipe B133 are installed in respectively the both sides of collimation lens set 134, male-pipe A132 is connected with the optical fiber that is positioned at cuvette device light light incident side by coupling fiber joint 15, and male-pipe B133 is connected with cylinder-shaped bracket wall 12; Described fiber optic collimator picks out joint 14 and comprises that internal thread sleeve pipe 141, male-pipe C142 and male-pipe D143(are referring to Fig. 7), the endoporus middle part of described internal thread sleeve pipe 141 is equipped with collimation lens B144, male-pipe C142 and male-pipe D143 are installed in respectively the both sides of collimation lens 144, male-pipe C142 is connected with the optical fiber that is positioned at cuvette device light emitting side by coupling fiber joint 15, and male-pipe D143 is connected with cylinder-shaped bracket wall 12.

Described coupling fiber joint 15 comprises that the ferrule 153 of fibre-optical splice nut 151, fiber orientation snap ring 152, rigidity and flexible optical fiber jacket 154(are referring to Fig. 8), described ferrule 153, optical fiber jacket 154 are coated on respectively outside the optical fiber, ferrule 153 is inserted in the through hole of male-pipe A132 or male-pipe C142, and spacing by fiber orientation snap ring 152, described fibre-optical splice nut 151 is installed in outside male-pipe A132 or the male-pipe C142 and with fiber orientation snap ring 152 and compresses.

The utility model proposes non-parallel light path transmission design and improve luminous flux, incident light enters from mobile outflow direction than the chrominance channel, adopt the combination of collimation lens set, focus is adjusted near mobile colorimetric feeder connection cross section I, can utilize so the larger area of colorimetric channel outlet cross section O that flows, import larger luminous flux; Be coupled into optical fiber through collimated again by the light of dispersing after the focus, import rearmounted light splitting and array electrooptical device (referring to Fig. 5).

During use, when cuvette adopts conventional cuvette, can realize the function of the visible spectrophotometry of general ultraviolet, can make common spectrophotometer and use; When adopting floating cuvette, the flow cell in the alternative traditional hyperchannel liquid chromatography photometric detector of this floating cuvette can be realized the function that liquid chromatography hyperchannel luminosity detects, and can be used as liquid chromatography hyperchannel luminosity detecting device and uses; When adopting floating cuvette and microsyringe sample introduction, can realize the function of the mobile colorimetric measurement of trace.

As a kind of conversion of embodiment one, described joint body 251 can also adopt stainless steel material to make.

Claims

1. cuvette device, it is characterized in that: comprise cuvette support (1) and can realize the cuvette that the visible spectrophotometry of general ultraviolet, liquid chromatography hyperchannel luminosity detect, trace flows the colorimetric measurement function, this cuvette is installed on the cuvette support (1).

2. cuvette device according to claim 1 is characterized in that: described cuvette comprises the conventional cuvette that can realize the visible spectrophotometry function of general ultraviolet and can realize the floating cuvette (2) that liquid chromatography hyperchannel luminosity detects, trace flows the colorimetric measurement function.

3. cuvette device according to claim 2, it is characterized in that: described floating cuvette (2) comprises airtight cuvette body (21), be provided with in the cuvette body (21) and flow than chrominance channel (22), this mobile diameter streamwise than chrominance channel (22) increases gradually; The top of cuvette body (21) is respectively equipped with and flows the sample feeding pipe (23) that is connected than chrominance channel (22) and reclinately go out sample pipe (24), go out the caliber of sample pipe (24) greater than the caliber of sample feeding pipe (23), sample feeding pipe (23) is connected with liquid chromatography inflow pipe (26) by pipe joint (25).

4. cuvette device according to claim 3, it is characterized in that: the sample feeding pipe (23) in the described floating cuvette (2), go out sample pipe (24) and flow all to adopt the fine pore of diameter≤2mm than the caliber of chrominance channel (22), sample feeding pipe (23), go out sample pipe (24) and flow more level and smooth than the inside of chrominance channel (22) and the junction is seamless.

5. cuvette device according to claim 3, it is characterized in that: described pipe joint (25) comprises joint body (251), this joint body (251) is fixed on the porch of sample feeding pipe (23), joint body (251) is provided with the connecting hole (252) that diameter increases gradually between liquid chromatography inflow pipe (26) and sample feeding pipe (23), joint body (251) also is provided with stepped hole (253) in the upper end, and be provided with external thread at the outer wall of stepped hole (253), clamp ring (254) for clamping liquid chromatography inflow pipe (26) is installed on the described stepped hole, and is equipped with for the pipe joint nut (255) that compresses clamp ring (254) and match with the external thread of joint body at clamp ring (254) top.

6. cuvette device according to claim 5 is characterized in that: described joint body (251) adopts Teflon or stainless steel material to make.

7. cuvette device according to claim 1, it is characterized in that: described cuvette support (1) comprises base (11), be fixed on the cylinder-shaped bracket wall (12) on the base (11), the cylinder-shaped bracket wall is placed with described cuvette in (12), described cylinder-shaped bracket wall (12) is equipped with fiber optic collimator access joint (13) at the light light incident side, at the light emitting side fiber optic collimator is installed and picks out joint (14), this fiber optic collimator access joint (13), fiber optic collimator picks out joint (14) and is connected with the optical fiber that is positioned at cuvette device (4) light light incident side and emitting side by coupling fiber joint (15) respectively.

8. cuvette device according to claim 7, it is characterized in that: described fiber optic collimator access joint (13) comprises internal thread sleeve pipe (131), male-pipe A(132) and male-pipe B(133), the endoporus middle part of described internal thread sleeve pipe (131) is equipped with collimation lens set (134), male-pipe A(132) and male-pipe B(133) be installed in respectively the both sides of collimation lens set (134), male-pipe A(132) be connected male-pipe B(133 by coupling fiber joint (15) with the optical fiber that is positioned at cuvette device light light incident side) be connected with cylinder-shaped bracket wall (12); Described fiber optic collimator picks out joint (14) and comprises internal thread sleeve pipe (141), male-pipe C(142) and male-pipe D(143), the endoporus middle part of described internal thread sleeve pipe (141) is equipped with collimation lens B(144), male-pipe C(142) and male-pipe D(143) be installed in respectively collimation lens B(144) both sides, male-pipe C(142) be connected male-pipe D(143 by coupling fiber joint (15) with the optical fiber that is positioned at cuvette device light emitting side) be connected with cylinder-shaped bracket wall (12).

9. cuvette device according to claim 8, it is characterized in that: described coupling fiber joint (15) comprises fibre-optical splice nut (151), fiber orientation snap ring (152), the ferrule of rigidity (153) and flexible optical fiber jacket (154), described ferrule (153), optical fiber jacket (154) is coated on respectively outside the optical fiber, ferrule (153) is inserted into male-pipe A(132) or male-pipe C(142) through hole in, and spacing by fiber orientation snap ring (152), described fibre-optical splice nut (151) is installed in male-pipe A(132) or male-pipe C(142) compress outward and with fiber orientation snap ring (152).