CN103698276B - A kind of fluorescence and ultraviolet-ray visible absorbing integration flow cell - Google Patents
A kind of fluorescence and ultraviolet-ray visible absorbing integration flow cell Download PDFInfo
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- CN103698276B CN103698276B CN201310701299.9A CN201310701299A CN103698276B CN 103698276 B CN103698276 B CN 103698276B CN 201310701299 A CN201310701299 A CN 201310701299A CN 103698276 B CN103698276 B CN 103698276B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/05—Flow-through cuvettes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6482—Sample cells, cuvettes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6484—Optical fibres
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- General Health & Medical Sciences (AREA)
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
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Abstract
The invention discloses a kind of fluorescence and ultraviolet-ray visible absorbing integration flow cell, described flow cell is made up of pond body and stall, liquid flow path is established in the body of described pond, described pond body is also provided with the feed tube and drain pipe that are connected with liquid flow path, the two ends of described liquid flow path are respectively light entrance and light outlet, described pond body is also arranged and light entrance and the perpendicular fluorescence window of light outlet, and Darkening process is all done in the position of pond body except light entrance, light outlet and fluorescence window; The position described stall corresponding to the light entrance of pond body, light outlet and fluorescence window arranges the first optical fiber interface, the second optical fiber interface, the 3rd optical fiber interface respectively, and between fluorescence window and three optical fiber interface corresponding with it, condenser lens is installed, for the light of fluorescence window is focused on the 3rd optical fiber interface.Flow cell of the present invention has highly sensitive, the advantage that parasitic light is little, is suitable for use in stratographic analysis field.
Description
Technical field
The invention belongs to technical field of chromatography, particularly, the present invention relates to a kind of fluorescence and ultraviolet-ray visible absorbing integration flow cell.
Background technology
In efficient liquid phase chromatographic analysis, it is two kinds of the most frequently used detection meanss that fluorescence and ultraviolet-ray visible absorbing detect.At present, conventional liquid phase chromatogram detects and adopts dedicated test pond, and user needs to configure respectively independently fluorescence detector and UV-Vis absorption detector and detects for fluorescence and ultraviolet-ray visible absorbing, safeguards increase all corresponding with use cost.Such as China's national standard to the aflatoxin fluorescence detection in milk, melamine uv detection method, cause relevant enterprise to need simultaneously to buy detection that fluorescence and UV-detector are respectively used to aflatoxin and melamine.If adopt fluorescence and ultraviolet-ray visible absorbing to detect the detecting device of one, then greatly can save maintenance and use cost.Because fluorescence is not identical with structure with the Cleaning Principle that ultraviolet-ray visible absorbing detects, be therefore difficult to by existing technology directly by different detection cell integrator.
Have the fluorescence of report and ultraviolet-ray visible absorbing integration flow cell at present while can detecting ultraviolet-ray visible absorbing, main employing 2 kinds of modes are collected fluorescence and are detected.One is a kind of absorbance and the synchronous on-line checkingi flow cell of fluorophotometric disclosed in Chinese patent 201010120953.3, adopt the mode of fluorescence optical fiber interface holes right angle vertical with light source spectrum fiber interface hole distribution, this mode can only detect the fluorescence of fluorescence optical fiber interface part, and the receiving surface of light is less; Another kind of flow cell as dual-purpose in ultraviolet-ray visible absorbing/fluorescent disclosed in Chinese patent 200610009153.8, the light sent from light source imports flow cell from one bifilar bifurcation fiber, derive fluorescence from another strand to detect, there is the problem that fluorescence receiving surface is little equally.
Because the requirement of current China to aspects such as food safety detection is more and more stricter, require lower to objectionable impurities detectability wherein, 1 μ g/kg is limited to, AFB in milk as total aflatoxin content in national Specification liquid chromatography-fluorescence detection mensuration food detects
1be 0.002 μ g/kg, AFB in milk powder
1be 0.02 μ g/kg.Therefore very high to the sensitivity requirement of fluorescence detector, and the fluorescent intensity that the little meeting of fluorescence receiving surface of existing detecting device causes detecting device to receive is more weak, is difficult to guarantee and meets the requirements of sensitivity.
Therefore, a kind of highly sensitive, fluorescence that structure is simply portable of exploitation and the dual-purpose flow cell of ultraviolet-ray visible absorbing is needed badly.
Summary of the invention
The object of the invention is to, provide a kind of fluorescence and ultraviolet-ray visible absorbing integration flow cell, this flow cell is simple and compact for structure, parasitic light is little, highly sensitive, can be used for fluorescence and ultraviolet-ray visible absorbing detects.
For achieving the above object, present invention employs following technical scheme:
Fluorescence of the present invention and ultraviolet-ray visible absorbing integration flow cell, comprise pond body 1 and the stall 2 for fixed spool body 1, liquid flow path 16 is provided with in described pond body 1, described pond body 1 is also provided with the feed tube 11 and drain pipe 12 that are connected with liquid flow path 16, the two ends of described liquid flow path 16 are respectively light entrance 13, light outlet 14, described pond body 1 is also provided with and light entrance 13 and the perpendicular fluorescence window 15 of light outlet 14, and Darkening process is all done in the position of described pond body 1 except light entrance 13, light outlet 14 and fluorescence window 15;
The position described stall 2 corresponding to pond body light entrance 13, light outlet 14 and fluorescence window 15 arranges the first optical fiber interface 21, second optical fiber interface 22, the 3rd optical fiber interface 23 respectively, and between fluorescence window 15 and three optical fiber interface 23 corresponding with it, condenser lens 24 is installed, for the light of fluorescence window 15 is focused on the 3rd optical fiber interface 23.
Preferably, described feed tube 11 and drain pipe 12 are arranged side by side, and those skilled in the art also can select other set-up modes as required.
Preferably, the making material of described pond body 1 is glass or quartz, and those skilled in the art also can select other to make material, such as organic glass (polymethylmethacrylate) etc. as required.
Fluorescence of the present invention and ultraviolet-ray visible absorbing integration flow cell are provided with fluorescence window 15, and by condenser lens 24, fluoroscopic examination is carried out in the gathering of the fluorescence of fluorescence window 15, therefore fluorescence receptor area is comparatively large, is provided with light outlet 14 simultaneously, detects for ultraviolet-ray visible absorbing.
Darkening process is all done in fluorescence of the present invention and the position of ultraviolet-ray visible absorbing integration flow cell pond body 1 except light entrance 13, light outlet 14 and fluorescence window 15, avoid light reflect in pond body 1 and spread, form parasitic light, interference fluorescence and ultraviolet-ray visible absorbing detect.
The invention has the advantages that, the present invention devises fluorescence and ultraviolet-ray visible absorbing light path on a pond body, increase fluorescence window, and fluorescence is focused on, Darkening process is carried out to non-light path part simultaneously, therefore had highly sensitive, the advantage that parasitic light is little, be suitable for use in stratographic analysis field, be especially suitable for portable high-efficiency liquid chromatograph
Accompanying drawing explanation
Fig. 1 is fluorescence of the present invention and ultraviolet-ray visible absorbing integration flow cell schematic diagram;
Fig. 2 is fluorescence of the present invention and ultraviolet-ray visible absorbing integration flow cell pond body schematic diagram;
Fig. 3 is fluorescence of the present invention and ultraviolet-ray visible absorbing integration flow cell stall schematic diagram;
Accompanying drawing identifies:
1, Chi Ti; 11, feed tube; 12, drain pipe; 13, light entrance; 14, light outlet; 15, fluorescence
Window; 16, liquid flow path; 2, stall; 21, the first optical fiber interface; 22, the second optical fiber interface; 23,
3rd optical fiber interface; 24, condenser lens.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described in detail.
As shown in Figure 1, fluorescence of the present invention and ultraviolet-ray visible absorbing integration flow cell, be provided with pond body 1 and the stall 2 for fixed spool body 1.
As shown in Figure 2, liquid flow path 16 is provided with in described pond body 1, described pond body 1 is also provided with the feed tube 11 and drain pipe 12 that are connected with liquid flow path 16, the two ends of described liquid flow path 16 are respectively light entrance 13, light outlet 14, described pond body 1 is also arranged and light entrance 13 and the perpendicular fluorescence window 15 of light outlet 14, and Darkening process is all done in the position of described pond body 1 except light entrance 13, light outlet 14 and fluorescence window 15;
As shown in Figure 3, the position described stall 2 corresponding to pond body light entrance 13, light outlet 14 and fluorescence window 15 arranges the first optical fiber interface 21, second optical fiber interface 22, the 3rd optical fiber interface 23 respectively, and between fluorescence window 15 and three optical fiber interface 23 corresponding with it, condenser lens 24 is installed, for the light of fluorescence window 15 is focused on the 3rd optical fiber interface 23.
Described feed tube 11 and drain pipe 12 are arranged side by side, and the making material of described pond body 1 is glass or quartz.
When fluorescence of the present invention and ultraviolet-ray visible absorbing integration flow cell carry out fluoroscopic examination, by the light of optical fiber guiding out light source, the fiber entrance 13 of pond body 1 is entered by the first optical fiber interface 21 of stall 2, sample in liquid flow path 16 is excited, the fluorescence excited is launched from fluorescence window 15, and after being focused on by condenser lens 24, enters the 3rd optical fiber interface 23, after being derived by optical fiber, carry out fluoroscopic examination by detecting device.
When fluorescence of the present invention and ultraviolet-ray visible absorbing integration flow cell carry out ultraviolet-ray visible absorbing detection, by the light of optical fiber guiding out light source, the fiber entrance 13 of pond body 1 is entered by the first optical fiber interface 21 of stall 2, by the absorption of sample part luminous energy in liquid flow path 16, penetrate from light outlet 14, enter the second optical fiber interface 22, after being derived by optical fiber, carry out ultraviolet-ray visible absorbing detection by detecting device.
Fluorescence of the present invention and ultraviolet-ray visible absorbing integration flow cell can carry out fluorescence and ultraviolet-ray visible absorbing detection simultaneously, by the light of optical fiber guiding out light source, the fiber entrance 13 of pond body 1 is entered by the first optical fiber interface 21 of stall 2, sample in liquid flow path 16 is excited, the fluorescence excited is launched from fluorescence window 15, and after being focused on by condenser lens 24, enters the 3rd optical fiber interface 23, after being derived by optical fiber, carry out fluoroscopic examination by detecting device; Through the light of liquid flow path 16 by absorption of sample part luminous energy wherein, penetrate from light outlet 14, enter the second optical fiber interface 22, after being derived by optical fiber, carry out ultraviolet-ray visible absorbing detection by detecting device.
It should be noted last that, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted.Although with reference to embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, modify to technical scheme of the present invention or equivalent replacement, do not depart from the spirit and scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (3)
1. a fluorescence and ultraviolet-ray visible absorbing integration flow cell, described flow cell comprises pond body (1) and the stall (2) for fixed spool body (1), it is characterized in that, liquid flow path (16) is established in described pond body (1), described pond body (1) is also provided with the feed tube (11) and drain pipe (12) that are connected with liquid flow path (16), the two ends of described liquid flow path (16) are respectively light entrance (13) and light outlet (14), described pond body (1) is also provided with and light entrance (13) and the perpendicular fluorescence window (15) of light outlet (14), pond body (1) is except light entrance (13), Darkening process is all done in position outside light outlet (14) and fluorescence window (15),
The position of the upper light entrance (13) corresponding to pond body (1) of described stall (2), light outlet (14) and fluorescence window (15) arranges the first optical fiber interface (21), the second optical fiber interface (22), the 3rd optical fiber interface (23) respectively, and between fluorescence window (15) and three optical fiber interface (23) corresponding with it, condenser lens (24) is installed, for the light of fluorescence window (15) is focused on the 3rd optical fiber interface (23).
2. fluorescence according to claim 1 and ultraviolet-ray visible absorbing integration flow cell, it is characterized in that, described feed tube (11) and drain pipe (12) are arranged side by side.
3. fluorescence according to claim 1 and ultraviolet-ray visible absorbing integration flow cell, it is characterized in that, the making material in described pond body (1) is glass or quartz.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310701299.9A CN103698276B (en) | 2013-12-18 | 2013-12-18 | A kind of fluorescence and ultraviolet-ray visible absorbing integration flow cell |
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| CN201310701299.9A CN103698276B (en) | 2013-12-18 | 2013-12-18 | A kind of fluorescence and ultraviolet-ray visible absorbing integration flow cell |
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| CN103698276B true CN103698276B (en) | 2016-01-20 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103969187A (en) * | 2014-05-07 | 2014-08-06 | 大连理工大学 | Fluorescence spectrophotometer flow cell assembly for fluorescent online quantitative detection |
| US9518923B1 (en) | 2015-12-07 | 2016-12-13 | International Business Machines Corporation | System and methods for fluorescence detection |
| CN110006859A (en) * | 2019-03-05 | 2019-07-12 | 北京雪迪龙科技股份有限公司 | A kind of fluorescent material detection device and the method for detecting fluorescent material |
| CN109932450B (en) * | 2019-04-03 | 2022-01-25 | 安徽皖仪科技股份有限公司 | Flow cell and liquid chromatograph with same |
| CN111650123A (en) * | 2020-06-22 | 2020-09-11 | 广东省测试分析研究所（中国广州分析测试中心） | Aflatoxin in-situ derived fluorescence detection device |
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