CN103969187A - Fluorescence spectrophotometer flow cell assembly for fluorescent online quantitative detection - Google Patents
Fluorescence spectrophotometer flow cell assembly for fluorescent online quantitative detection Download PDFInfo
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- CN103969187A CN103969187A CN201410189160.5A CN201410189160A CN103969187A CN 103969187 A CN103969187 A CN 103969187A CN 201410189160 A CN201410189160 A CN 201410189160A CN 103969187 A CN103969187 A CN 103969187A
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Abstract
The invention belongs to the field of equipment for fluorescence spectrum test analysis, and relates to a fluorescence spectrophotometer flow cell assembly for fluorescent online quantitative detection. The fluorescence spectrophotometer flow cell assembly is 5-10 mm in sum of length and width, not less than 40 mm in height and 1 mm in wall thickness; a liquid inlet is formed in a side surface of a flow cell, a liquid outlet is formed in the top end of the flow cell, a fluorescence excitation light inlet and a fluorescence emission light outlet are formed in the middle of the adjacent side surface of the liquid inlet and the middle of the opposite side surface of the liquid inlet respectively, and the fluorescence excitation light inlet is higher than the upper edge of the liquid inlet by not less than 10 mm; the light shading treatment is carried out; a flow meter is connected to a liquid inlet pipe and used for controlling the flow speed of liquid entering the flow cell. The fluorescence spectrophotometer flow cell assembly is convenient to mount, can achieve real-time online quantitative detection within the liquid flow speed range of 0-100 mL/min, and is stable and reliable in detection result, good in repeatability and suitable for real-time online detection of a fluorescence intensity change process.
Description
Technical field
The invention belongs to fluorescence spectrum test analysis apparatus field, relate to a kind of for the online fluorospectrophotometer flow cell assembly quantitatively detecting of fluorescence.
Background technology
Fluorospectrophotometer is a kind of instrument for scanning the fluorescence spectrum that liquid phase fluorescent marker sends, belong to the one in spectrophotometer, there is the advantages such as highly sensitive, selectivity good, few by sample amount, method is easy, be widely used in the fields such as material, chemical industry, environmental protection, medicine and life science, can have carried out to protein, medicine, agricultural chemicals and heavy metal etc. the mensuration of quantitative test and fluorescence spectrum.Existing fluorescent sub-photometer is desk-top instrument, when measurement, fluid sample need to be transferred in sample cell and measured, need to detect online for many application, or require the research work of Real-Time Monitoring change in fluorescence process, can not provide quickly testing result.
The disclosed a kind of fluorospectrophotometer for online or remote measurement of Chinese patent 200710058310.9, comprise light source, exciting light monochromator, fluorescence monochromator and fiber transmission device, this fluorospectrophotometer uses for independent, can not be connected on existing commercially available fluorospectrophotometer, be unfavorable for applying of the online measuring ability of fluorescence.
Summary of the invention
For the problem of above-mentioned existence, in order to develop the online quantitatively measuring ability of fluorospectrophotometer, and can support the use with existing commercially available fluorospectrophotometer, the present invention proposes the online fluorospectrophotometer flow cell assembly quantitatively detecting of a kind of fluorescence.
In order to achieve the above object, the present invention has adopted following technical scheme:
The invention provides a kind of fluorospectrophotometer flow cell assembly for the online quantitative detection of fluorescence, mainly comprise feed tube 1, inlet 2, flow cell 3, liquid outlet 4, flowmeter 6, fluorescence excitation light entrance 8 and fluorescent emission light exit 9; The length of flow cell 3 and the wide 5-10mm of being, be highly not less than 40mm, wall thickness is 1mm, inlet 2 is positioned at a side of flow cell 3, liquid outlet 4 is on the top of flow cell 3, fluorescence excitation light entrance 8 and fluorescent emission light exit 9 lay respectively at the middle part of inlet 2 adjacent side and the middle part of inlet 2 opposite flanks, and fluorescence excitation light entrance 8 is not less than 10mm with the coboundary distance of inlet 2; The pond body of described flow cell 3 is except fluorescence excitation light entrance 8 and fluorescent emission light exit 9, and other parts are carried out lucifuge processing.Connection traffic meter 6 on feed tube 1, to control the flow velocity of the pond liquid that circulates.
Preferably, adopt metal support 7 and supporting shell 10 to be fixed and lucifuge the pond body of flow cell 3, those skilled in the art also can adopt the fixing flow cell body of other modes lucifuge as required.
Preferably, on supporting shell 10 of the present invention, adopt metal two logical 11 to connect feed tube 1/ drain pipe 5 and testing liquid pipeline, so that pipeline dismounting and cleaning.Those skilled in the art also can adopt other modes to connect feed tube/drain pipe and testing liquid pipeline as required.
Preferably, the material of flow cell 3 of the present invention is quartz or glass, and those skilled in the art also can select other to make material as required, as organic glass (polymethylmethacrylate) etc.
The invention has the beneficial effects as follows the side of inlet at flow cell, and fluorescence excitation light entrance and fluorescent emission light exit are respectively at the middle part of inlet adjacent side and opposite flank, and with inlet coboundary distance >=10mm, fully to reduce the impact of flow of solution on measurement result.This assembly can be connected with any flow-type experimental provision the online quantitatively detection for fluid sample fluorescence intensity, at fluid sample concentration 100-1000 μ g/L, testing result and fluorospectrophotometer Static Analysis Method there was no significant difference within the scope of flow rate of liquid 0-100mL/min, there is measurement result reliable and stable, the feature of favorable reproducibility, the fluorescence real-time online that can be widely used in the fields such as chemical-biological detects.
Brief description of the drawings
Fig. 1 is primary structure schematic diagram of the present invention.
Fig. 2 is primary structure sectional view of the present invention.
In figure: 1 feed tube; 2 inlets; 3 flow cells; 4 liquid outlets; 5 drain pipes; 6 flowmeters; 7 metal supports; 8 fluorescence excitation light entrances; 9 fluorescent emission light exits; 10 supporting shells; 11 metals two are logical.
Embodiment
Further illustrate the specific embodiment of the invention below with reference to accompanying drawing and technical scheme content.
Embodiment 1
Online structure and the working method that quantitatively detects flow cell assembly of fluorescence
The online flow cell assembly that quantitatively detects of described fluorescence, is mainly made up of feed tube 1, inlet 2, flow cell 3, liquid outlet 4, drain pipe 5, flowmeter 6, metal support 7, fluorescence excitation light entrance 8, fluorescent emission light exit 9, supporting shell 10 and metal two logical 11.Preferably, the specification of flow cell 3 is 10 × 10 × 45mm (internal diameter), wall thickness 1mm, inlet 2 diameter 5mm, its center is apart from the about 8mm in sidewall bottom, excitation source centre distance bottom 20mm, liquid outlet 4 is at flow cell top, diameter 5mm, adopt metal support flow cell is fixed and be connected with feed tube, drain pipe, adopt peristaltic pump that testing liquid is passed through to flow cell with certain flow rate, adopt flowmeter 6 to control flow, and carry out as required fluorescence and detect online.Wherein, the size shape of metal support 7 and supporting shell 10 is not limited to the structure shown in accompanying drawing 1 of the present invention, can determine according to the specification of supporting fluorospectrophotometer.
Embodiment 2
The reliability of the online quantitative detecting method of fluorescence
Taking antibiotic medicine Ciprofloxacin as example, the reliability of fluorescence on-line quantitative analysis method is described.The maximum excitation wavelength (Ex) of Ciprofloxacin is 280nm, and optimum transmit wavelength (Em) is 445nm, prepares respectively the determinand solution 2L of 100,250,500,750,1000 μ g/L, carries out quantitative test under spectral scan pattern.
First group of experiment, from above-mentioned solution, taking out respectively 2mL is placed in cuvette and measures its fluorescence intensity under maximum excitation wavelength and optimum transmit wavelength, second group of experiment, under peristaltic pump effect, make the testing liquid online flow cell assembly that quantitatively detects of fluorescence of flowing through, and measure its fluorescence intensity under maximum excitation wavelength and optimum transmit wavelength, by two groups of experimental data contrasts, found that, within the scope of concentration 100-1000 μ g/L, two groups of experimental result differences are in ± 5% scope, illustrate that the online quantitative detecting method of fluorescence has reliability in this concentration range.
Embodiment 3
The stability of the online quantitative detecting method of fluorescence
Taking antibiotic medicine Ciprofloxacin as example (Ex=280nm, Em=445nm), concentration is measured the fluorescence intensity under different in flow rate within the scope of 100-1000 μ g/L, measure respectively flow rate of liquid 0,10,25,50 and 100mL/min condition under fluorescence intensity, measurement result difference under discovery different in flow rate condition, in ± 5% scope, illustrates that the measurement result of the online quantitative detecting method of fluorescence has certain stability.
Claims (5)
1. for the online fluorospectrophotometer flow cell assembly quantitatively detecting of fluorescence, comprise feed tube, inlet, flow cell, liquid outlet, flowmeter, fluorescence excitation light entrance and fluorescent emission light exit; It is characterized in that, the length of described flow cell and the wide 5-10mm of being, be highly not less than 40mm, wall thickness is 1mm; Inlet is positioned at a side of flow cell, and liquid outlet is positioned at the top of flow cell, and fluorescence excitation light entrance and fluorescent emission light exit lay respectively at the middle part of inlet adjacent side and the middle part of inlet opposite flank; Fluorescence excitation light entrance is not less than 10mm higher than the coboundary of inlet; The pond body of described flow cell is except fluorescence excitation light entrance and fluorescent emission light exit, and other parts are carried out lucifuge processing; Connection traffic meter on feed tube, to control the flow velocity of the pond liquid that circulates.
2. a kind of fluorospectrophotometer flow cell assembly for the online quantitative detection of fluorescence according to claim 1, is characterized in that, described lucifuge is processed and adopted metal support and supporting shell to be fixed and lucifuge the pond body of flow cell.
3. a kind of fluorospectrophotometer flow cell assembly for the online quantitative detection of fluorescence according to claim 1 and 2, is characterized in that, adopts metal two to lead to and connect feed tube/drain pipe and testing liquid pipeline on described supporting shell.
4. a kind of fluorospectrophotometer flow cell assembly for the online quantitative detection of fluorescence according to claim 1 and 2, is characterized in that, the material of described flow cell is quartz, glass or organic glass.
5. a kind of fluorospectrophotometer flow cell assembly for the online quantitative detection of fluorescence according to claim 3, is characterized in that, the material of described flow cell is quartz, glass or organic glass.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410189160.5A CN103969187A (en) | 2014-05-07 | 2014-05-07 | Fluorescence spectrophotometer flow cell assembly for fluorescent online quantitative detection |
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| CN201410189160.5A CN103969187A (en) | 2014-05-07 | 2014-05-07 | Fluorescence spectrophotometer flow cell assembly for fluorescent online quantitative detection |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106979936A (en) * | 2016-01-18 | 2017-07-25 | 南开大学 | It is a kind of for water quality detection without tube wall flow cell device |
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| CN1815218A (en) * | 2006-02-15 | 2006-08-09 | 厦门大学 | Ultraviolet-ray visible absorbing/fluorescent dual-purpose flow cell |
| CN2833580Y (en) * | 2005-07-07 | 2006-11-01 | 福州大学 | Sensing film fluorescence analysis flow cell |
| CN101135641A (en) * | 2007-07-19 | 2008-03-05 | 天津大学 | Fluorescence spectrophotometer on-line or remote measurement |
| CN101776574A (en) * | 2010-02-09 | 2010-07-14 | 新疆医科大学 | Flow cell device suitable for synchronous on-line detection of absorption spectrum and fluorescence spectrum |
| CN201637663U (en) * | 2010-02-09 | 2010-11-17 | 新疆医科大学 | Flow-through cell |
| WO2012144955A1 (en) * | 2011-04-21 | 2012-10-26 | Massachusetts Institute Of Technology | Highly compact multi-optical-junction optical flowcell and flexibly deployable optical sensing assemblies and systems for in-situ real-time spectroscopic measurements |
| CN103698276A (en) * | 2013-12-18 | 2014-04-02 | 中国科学院过程工程研究所 | Fluorescence and ultraviolet-visible absorption integrated flow cell |
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Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2833580Y (en) * | 2005-07-07 | 2006-11-01 | 福州大学 | Sensing film fluorescence analysis flow cell |
| CN1815218A (en) * | 2006-02-15 | 2006-08-09 | 厦门大学 | Ultraviolet-ray visible absorbing/fluorescent dual-purpose flow cell |
| CN101135641A (en) * | 2007-07-19 | 2008-03-05 | 天津大学 | Fluorescence spectrophotometer on-line or remote measurement |
| CN101776574A (en) * | 2010-02-09 | 2010-07-14 | 新疆医科大学 | Flow cell device suitable for synchronous on-line detection of absorption spectrum and fluorescence spectrum |
| CN201637663U (en) * | 2010-02-09 | 2010-11-17 | 新疆医科大学 | Flow-through cell |
| WO2012144955A1 (en) * | 2011-04-21 | 2012-10-26 | Massachusetts Institute Of Technology | Highly compact multi-optical-junction optical flowcell and flexibly deployable optical sensing assemblies and systems for in-situ real-time spectroscopic measurements |
| CN103698276A (en) * | 2013-12-18 | 2014-04-02 | 中国科学院过程工程研究所 | Fluorescence and ultraviolet-visible absorption integrated flow cell |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106979936A (en) * | 2016-01-18 | 2017-07-25 | 南开大学 | It is a kind of for water quality detection without tube wall flow cell device |
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Application publication date: 20140806 |