CN106546562A - A kind of microbiological sensor and turbidity detection method based on Turbidity measurement - Google Patents
A kind of microbiological sensor and turbidity detection method based on Turbidity measurement Download PDFInfo
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- CN106546562A CN106546562A CN201611127105.9A CN201611127105A CN106546562A CN 106546562 A CN106546562 A CN 106546562A CN 201611127105 A CN201611127105 A CN 201611127105A CN 106546562 A CN106546562 A CN 106546562A
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- light
- leaded light
- turbidity measurement
- sensor based
- light device
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
- G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
Abstract
The present invention relates to a kind of microbiological sensor and turbidity detection method based on Turbidity measurement.The microbiological sensor based on Turbidity measurement of the present invention, including light source, the first leaded light device, the second leaded light device, detector and the container for placing determinand；The exciting light that light source sends after the first leaded light device is received by determinand and by the second leaded light device, is finally transferred into detector.Its advantage is：Compared with 90 degree of scattered lights in traditional Turbidity measurement are detected, using double leaded light devices detection scattered lights side by side in the present invention, concentrate with light intensity, receiving sensitivity height, and the characteristics of strong antijamming capability；Compared with traditional colony counting method, due to the optical power value that leaded light device is received, it is the combined reaction of all microorganisms in the facula area to exciting light, therefore there is quick detection；Compared with current other method for quick, the present invention is direct detection bacterium colony, therefore more directly perceived, reliable.
Description
Technical field
The present invention relates to a kind of microbiological sensor and turbidity detection method based on Turbidity measurement, can be applicable to biochemical point
The fields such as analysis, biomedical and Food Inspection.
Background technology
Turbidity measurement (detecting the content of suspended particulate substance) based on light scattering effect is the conventional side in biochemical analysis
One of method.Scattering causes the direction of propagation deflection (angle of deflection is referred to as " angle of scattering ") of light beam, generally adopts 90 degree of angle of scatterings
(i.e. vertical with incident light direction) detects scattered light, so as to scattered light is separated with transmitted light.But, angle of scattering be 0～
It is distributed between 360 degree, 90 degree are detected the scattered light that can only collect fraction, and detection efficiency is low, it is therefore desirable to extremely highly sensitive
Photoelectric detector (such as avalanche multiplication photoelectric tube, single photon detector etc.)；Also, scattered light is very faint, environment veiling glare it is dry
Disturb big.
Meanwhile, in field of food safety, the most frequently used microorganism detection method is：The sample of sampling is put in culture medium
Culture, makes single microbial grow into a bacterium colony, and the content of microorganism can be known by calculating the number of bacterium colony.But bacterium
The developmental process for falling is very slow (generally needing 24～48 hours), therefore detection process is very time-consuming.Meanwhile, counting method needs one by one
The quantity of statistics microorganism, it is also very time-consuming.And, the usual very little of microorganism, needs can just be seen under the microscope, and microscope
Observation visual field very little, it is impossible to check interior in a big way microbial profile situation, this can bring statistical error.
In order to improve the detection speed of microorganism, the method for adopting at present mainly has：ATP methods, immunization, impedance method and
Chromogenic culture medium method etc..But these methods are indirectly testing (such as the metabolins of test microbes), rather than microorganism is carried out
The shortcomings of directly observing, therefore there is big test error and false positive.
To sum up, design a kind of new turbidity detection method so as to meet the quick detection of microorganism, and have highly sensitive
Degree, high accuracy and low cost, are that the wound of the present invention grinds motivation.
The content of the invention
It is an object of the invention to overcome the deficiencies in the prior art, there is provided one kind can realize quick detection, and have height
The microbiological sensor based on Turbidity measurement of sensitivity, high accuracy and low cost and turbidity detection method.
A kind of microbiological sensor based on Turbidity measurement of the present invention, its technical scheme is：
A kind of microbiological sensor based on Turbidity measurement, including light source, the first leaded light device, the second leaded light device, inspection
Survey device and the container for placing determinand；The exciting light that light source sends after the first leaded light device incides determinand,
Two leaded light devices receive scattered light and transmission of stray light are entered detector.
Wherein, microbiological sensor is except for microorganism detection, can be additionally used in Detection of Air Quality (such as haze), thin
Detection of born of the same parents and protein etc..
Wherein, liquid determinand can be placed in container (for example pour liquid determinand in container into), the liquid of container
Culture medium, when microorganism does not grow, its optical characteristics is evenly distributed.When there is particulate matter in determinand, particulate matter can be scattered
Part exciting light simultaneously changes its transmission direction (between 0～360 degree), and the scattered light of wherein 180 degree angle of scattering is most strong.Therefore, lead to
After crossing determinand, exciting light is divided into：Change " scattered light " of transmission direction, and keep the " transmission of original transmission direction
Light ".
Wherein, the optical fiber is single-mode fiber, multimode fibre, tapered fiber or microstructured optical fibers.
Wherein, first leaded light device is metal capillary, optical fiber or optical fiber collimator, and second leaded light device is
Metal capillary, optical fiber or optical fiber collimator.
Wherein, when the first leaded light device or the second leaded light device select optical fiber, the first leaded light device and the second lightguide
Be provided with optical confinement layer between part, optical confinement layer can be flake, sleeve-shaped or directly in optical fiber surface plated film, for every
(i.e. when determinand does not have particle to be scattered, exciting light can not be guide-lighting into second for optical interference between double leaded light devices
Device), its material can be metal, ceramics or organic polymer, wherein it is preferred that metal material.
Wherein, the thickness of optical confinement layer is 50nm to 5mm.
Wherein, the first leaded light device and the second leaded light device parallel arranged, the first leaded light device and the second leaded light device
It is close to (end distance of the two is less than 5mm) in port.Between the angle of the two is in 0 degree to 60 degree, preferably 0 degree angle is (now,
Two is arranged in parallel between optical fiber or metal capillary)；It is close to the port of first and second optical fiber (or metal capillary).When
When first leaded light device and the second leaded light device have angle, the angle of the first leaded light device and the second leaded light device is 0.0001
Spend to 60 degree.
Wherein, determinand is the culture medium containing microorganism, and light source is light emitting diode, laser instrument or broad spectrum light source
(such as halogen tungsten lamp), detector are photodetector or CCD imager part.
Wherein, the preferred infrared light of exciting light or ultraviolet light, compared with visible ray, absorption of the water to infrared light (or ultraviolet light)
Loss is big, can be attenuated to relatively large extent when exciting light is transferred to container side wall, the interference of stray light produced by corresponding side wall reflection
Can reduce.
The detection method of a kind of microbiological sensor based on Turbidity measurement of the present invention, by the training containing microbiological specimens
Foster base is poured in container (optional cuvette), and the first leaded light devices of exciting light Jing that light source sends are irradiated to the determinand in container
On, the second leaded light device receives scattered light, and transmission of stray light to detector；As the microbes scattering in container is excited
Light, the detection changed to realize microorganism of the optical power value by analyzing scattered light.As the microbes in container are scattered
(growth of microorganism can cause indicative of local optical property in culture medium, such as light refractive index, absorptivity, thickness or surface to exciting light
The change of roughness etc.), the detection changed to realize microorganism of the optical power value by analyzing scattered light.
The enforcement of the present invention is with following technique effect：
Particle scattering can change the transmission of angle of light beam so that " exciting light " is divided into " scattered light " and " transmitted light ".Pass
The Turbidity measurement of system, only detects a fraction of " scattered light " (scattered lights such as 90 degree), and the scattered light of other angles then by
Waste, therefore detection efficiency is low, and easily receive environmental disturbances.The detection mode of the present invention is detection scattered light, due to adopting
Double optical fiber (or metal capillary) side by side as leaded light device, can along the propagation path collection of scattered light of exciting light, because
This scattering light collection efficiency is high；Also, due to optical confinement layer, can phase between the port of double optical fiber (or metal capillary)
Mutually near and be close to (its spacing can be less than 5mm), therefore scattered light can be collected to greatest extent, and eliminate exciting light
Impact to scattered light, it is high with sensitivity.The characteristics of strong antijamming capability.
The detection method of the microbiological sensor based on scattering loss that the present invention is provided, with traditional bacterium colony culture and meter
Counting method is compared, and what is received due to optical fiber collimator is optical power value (intensity of light is relevant with microorganism concn), is to swashing
The combined reaction (not being to count one by one) of all microorganisms in luminous facula area, therefore compared with colony counting method, have
The advantage of quick detection；With current other method for quick (such as ATP methods, immunization, impedance method and chromogenic culture medium method
Deng indirect test method) to compare, the method for the present invention is direct detection bacterium colony, therefore more directly perceived, reliable.
Description of the drawings
Fig. 1 is a kind of schematic diagram of microbiological sensor based on Turbidity measurement of the present invention.
Specific embodiment
The present invention is described in detail below in conjunction with embodiment and accompanying drawing, it should be pointed out that described reality
Apply example and be intended merely to facilitate the understanding of the present invention, and any restriction effect is not risen to which.
Embodiment 1
It is shown in Figure 1, the present embodiment provide a kind of microbiological sensor based on Turbidity measurement, including light source 1, by
The first optical fiber 2 that two optical fiber is constituted side by side and the second optical fiber 3, optical confinement layer 15, detector 9, and for placing liquid training
The cuvette 13 of foster base 14.Wherein, the exciting light 4 that light source sends is through the first optical fiber 2, the particulate matter being irradiated in culture medium 14
On 8, Jing after particulate matter 8 is scattered, scattered light 12 is received by the second optical fiber 3 and is transferred to detector 9.
Optical confinement layer 15 between optical fiber can be separated (i.e. exciting light by the light beam in the first optical fiber 2 and the second optical fiber 3
Separate with scattered light), it is to avoid interfere.Simultaneously as the exciting light of the first fibre optical transmission is diverging, in fiber port
The luminous intensity at place is maximum.As optical confinement layer very thin (thickness 50nm to 5mm), the port of the second optical fiber 3 and the first optical fiber 2 can be with
It is close to, therefore the second optical fiber 3 can receives scattered light where detection luminous intensity maximum, to the collection efficiency of scattered light most
It is high.Further, since the orientation of the first optical fiber 2 and the second optical fiber 3 be close to it is parallel, detection light whole transmission path L on,
The scattered light (i.e. the scattered light of angle of scattering about 180 degree) for being excited all may be received by the second optical fiber 3.Therefore, with it is traditional
Microbiological sensor is compared, and the present embodiment has advantages below：1. can detect scattered light in whole transmission path (by receive
The scattering light distribution path of collection is longer)；2. the 180 degree scattered light collected by is more higher than the scattered light of other angles；3. can be
Scattered light is collected at the maximum fiber port of light intensity；4. optical fiber has soft, flexible feature, can reduce volume.Therefore,
The characteristics of with high collection efficiency, high sensitivity and small volume.
It is in order to the above-mentioned microbiological sensor based on scattering loss and detection method is discussed in greater detail, following with enforcement
Example is described in detail：
As shown in figure 1, liquid culture medium 14 (culture medium contain extract from testing sample microorganism) is poured into
In bright cuvette 13.
The exciting light 4 that light source 1 sends, incides in liquid culture medium 14 through the first optical fiber 2, is cultured particle 8 in base
Scattering, scattered light 12 is into the second optical fiber 3 and is transferred into by photodetector 9.Wherein in the first optical fiber 2 and the second optical fiber 3,
Inserting thickness is the stainless steel thin layer 15 of 0.1mm as optical confinement layer.
When the microorganism in liquid culture medium 14 starts to grow into bacterium colony 8, the light refractive index in 8 region of bacterium colony occurs to become
Change, and exciting light 4 is formed scatter so that deflected by the transmission of angle of the segment beam of bacterium colony 8.Wherein, entirely propagating
On the L of path, the scattered light 12 of all about 180 degree angle of scatterings can be received by the second optical fiber 3.The changed power of scattered light 12,
It is the coefficient result of all microorganisms in the range of hot spot, therefore without the need for count to single microbial, with quick inspection
The characteristics of survey.
Embodiment 2
The present embodiment difference from Example 1 is to change optical fiber into metal capillary, the present embodiment only to enforcement
The different structure of example is illustrated, and identical part will not be described in great detail.The present embodiment adopts the first metal capillary and the second gold medal
Category capillary replaces the first optical fiber 2 and the second optical fiber 3, is transmitted detection light and collects scattered light.As light beam is in metal
Capillary internal transmission, the side wall of capillary can effective the reflected beams, therefore capillary tube side wall can isolate detection light and dissipate
Penetrate light；Now, between two capillaries, optical confinement layer need not be adopted.The enforcement of the present embodiment can be reached as embodiment 1
Technique effect.
Finally it should be noted that above example is only illustrating technical scheme, rather than to present invention guarantor
The restriction of shield scope, although having made to explain to the present invention with reference to preferred embodiment, one of ordinary skill in the art should
Work as understanding, technical scheme can be modified or equivalent, without deviating from the reality of technical solution of the present invention
Matter and scope.
Claims (11)
1. a kind of microbiological sensor based on Turbidity measurement, it is characterised in that：Lead including light source, the first leaded light device, second
Optical device, detector and the container for placing determinand；The exciting light that light source sends after the first leaded light device, by treating
Survey thing and received by the second leaded light device, be finally transferred into detector.
2. a kind of microbiological sensor based on Turbidity measurement according to claim 1, it is characterised in that：The microorganism
Sensor is used to detect microorganism, air hazardous material content, cell or protein.
3. a kind of microbiological sensor based on Turbidity measurement according to claim 1, it is characterised in that：Described first leads
Optical device and second leaded light device are metal capillary, optical fiber or optical fiber collimator.
4. a kind of microbiological sensor based on Turbidity measurement according to claim 3, it is characterised in that：When first guide-lighting
When device or the second leaded light device select optical fiber, between the first leaded light device and the second leaded light device, optical confinement layer is provided with.
5. a kind of microbiological sensor based on Turbidity measurement according to claim 4, it is characterised in that：The optical fiber is
Single-mode fiber, multimode fibre, tapered fiber or microstructured optical fibers.
6. a kind of microbiological sensor based on Turbidity measurement according to claim 4, it is characterised in that：It is described optically isolated
The thickness of layer is 50nm to 5mm.
7. according to a kind of arbitrary described microbiological sensor based on Turbidity measurement of Claims 1 to 5, it is characterised in that：The
One leaded light device and the second leaded light device parallel arranged, the first leaded light device are close to the port of the second leaded light device.
8. according to a kind of arbitrary described microbiological sensor based on Turbidity measurement of Claims 1 to 5, it is characterised in that：The
The angle of one leaded light device and the second leaded light device is 0.0001 degree to 60 degree.
9. a kind of microbiological sensor based on Turbidity measurement according to claim 6, it is characterised in that：Exciting light is selected
Infrared light or ultraviolet light, using water to infrared light or the absorption loss of ultraviolet light, interference of stray light caused by drop low sidewall reflection.
10. a kind of microbiological sensor based on Turbidity measurement according to claim 6, it is characterised in that：Determinand is
Liquid culture medium containing microorganism；Light source is light emitting diode, laser instrument or halogen tungsten lamp；Detector be photodetector or
CCD imager part.
A kind of 11. detection methods of the microbiological sensor based on Turbidity measurement, it is characterised in that：By containing microbiological specimens
Culture medium is poured in container, and the first leaded light devices of exciting light Jing that light source sends are irradiated on the determinand in container, and second leads
Optical device receives scattered light, and transmission of stray light to detector；Due to the microbes scattering exciting light in container, by dividing
The detection changed to realize microorganism of the optical power value of analysis scattered light.
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| CN201611127105.9A CN106546562A (en) | 2016-12-08 | 2016-12-08 | A kind of microbiological sensor and turbidity detection method based on Turbidity measurement |
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Cited By (2)
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| CN107356596A (en) * | 2017-07-11 | 2017-11-17 | 南京工程学院 | Underwater spotting imaging device and optical imagery method water turbidity online test method |
| CN110567902A (en) * | 2018-06-06 | 2019-12-13 | 中国科学院长春光学精密机械与物理研究所 | Mould detection device and detection method |
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Application publication date: 20170329 |