CN104165875A - Support applied to testing of solid-state fluorescence samples - Google Patents
Support applied to testing of solid-state fluorescence samples Download PDFInfo
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- CN104165875A CN104165875A CN201410373730.6A CN201410373730A CN104165875A CN 104165875 A CN104165875 A CN 104165875A CN 201410373730 A CN201410373730 A CN 201410373730A CN 104165875 A CN104165875 A CN 104165875A
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- support
- gas
- fluorescence
- cuvette
- sample stage
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Abstract
The invention relates to a support applied to testing of solid-state fluorescence samples. The support comprises a support sealing cover, a support body, a cuvette clamping ring, a clamping groove, a sample stage, a gas inlet guide pipe and a gas outlet guide pipe, wherein the support sealing cover and the sample stage are positioned on the top side and the tail side of the support body, respectively; the cuvette clamping ring penetrates through the support body to be fixed under the support sealing cover; a dihedral angle alpha between the plane of the sample stage and a horizontal plane is larger than 90 degrees and smaller than 180 degrees; the gas inlet and outlet guide pipes are arranged in the support body, and openings of the gas inlet and outlet guide pipes are formed above the sample stage and in the bottom of the support body, respectively. The sample support is a general solid-state fluorescence testing accessory, can be applied to an on-line fluorescence sensing test for gas in real time, and can improve the accuracy of the gas sensing test. A negative pressure suction way is adopted to introduce gas to be tested into a gas chamber composed of the support and a general fluorescence cuvette, and the risk of toxic or harmful gas leaking in the testing process can be effectively reduced.
Description
Technical field
The present invention relates to solid fluorescence sensory field, particularly a kind of support for solid fluorescence sample test.
Background technology
The fluorescence sense method changing based on fluorescence molecule fluorescence spectrum is because its high sensitivity and high selectivity have obtained the extensive concern of scientific research institution and associated companies.The screening of fluorescence sense molecule is the Focal point and difficult point of exploitation fluorescent optical sensor.Realizing solid fluorescence material is the screening of fluorescent sensing material to the sensing Focal point and difficult point of specific gas, and the problem that first will solve is to realize the diffusion of gas to be measured on fluorescent sensing material surface.Although at present commercially available fluorospectrophotometer and spectrometer have all configured the test bracket of solid sample, all can not real-time continuous realize the monitoring to gas to be measured.According to bibliographical information, the research method generally adopting is at present sensing membrane material to be immersed in the saturated vapour of gas to be measured to certain hour transfer to and on spectrum platform, test its fluorescence spectrum.Due to when test sensing material be exposed to air, be difficult to avoid the volatilization diffusion of gas to be measured and the measuring error that causes.In order to adapt to the demand of spectrum test, publishedly can mainly be divided into two classes for the patent of spectral measurement: the one, solid fluorescence test accessories, can measure Solid fluorescene spectrum but whole annex is exposed in air and can not carries out the sensing testing (CN200953001Y for gas, CN201378142Y, CN202869978U); Another kind is the air chamber class testing annex that spectral absorption own is measured based on gas, although can pass into gas, the position (CN102103072A, CN103454222A) of placing solid fluorescence sensing material is not set.Therefore thirst for finding a kind of solid fluorescence sensing testing solution of simple general-purpose.
Summary of the invention
Carry out the fluorescence sense test for gas in order to solve solid fluorescence sample, the invention provides a kind of support for solid fluorescence sample test, can be provided in the general solid fluorescence sensing testing solution realizing on most of fluorospectrophotometers and spectrometer according to the present invention.
The present invention solves the scheme that its technical matters adopts: a kind of support for solid fluorescence sample test, solid fluorescence sample is equipped on support, again support is inserted in general fluorescence cuvette, by air induction conduit and outtake tube, gas to be measured is introduced in fluorescence cuvette solid fluorescence sample is tested.
The lower end of the stake body 2 of this support is provided with sample stage 10, the top of stake body 2 is support gland bonnet 3, cuvette snap ring 7 is embedded in below support gland bonnet 3 through stake body 2, and with support gland bonnet 3 fixing compacts, in the draw-in groove 8 of O-ring seal 6 between cuvette snap ring 7 and stake body 2; The air inlet openings 9 of air induction conduit 5 be positioned at sample stage 10 vertical directions directly over, air induction conduit 5 connects support gland bonnet 3 and stake body 2, is coupled to air inlet openings 9; The opening 1 of giving vent to anger of outtake tube 4 is positioned at stake body 2 bottoms, and outtake tube 4 connects support gland bonnet 3 and stake body 2, is coupled to the opening 1 of giving vent to anger.
Support of the present invention, is characterized in that the plane of sample stage 10 and the dihedral angle α that surface level forms are greater than 90 °, are less than 180 °.
The invention has the beneficial effects as follows, can carry out the fluorescence sense test for gas real-time continuous to be measured to solid fluorescence sample, having saved in the saturated vapour that solid fluorescence sample is immersed in gas to be measured certain hour transfers to and on spectrum platform, tests this step, reduce the volatilization diffusion of gas to be measured, improved the degree of accuracy of test.Use support gland bonnet 3 and O-ring seal 6 by tight to general fluorescence cuvette and the sealing of solid sample support, while carrying out fluorescence sense test, use aspiration pump, in the sealed gas chamber that fluorescence cuvette is become with solid sample holder combination, be negative pressure state, in test process, gas to be measured can not be revealed, and guarantees operator safety.
Brief description of the drawings
Fig. 1 is a kind of structural representation of the support for solid fluorescence sample test.
Fig. 2 is a kind of use process flow diagram of the support for solid fluorescence sample test.
1: the opening of giving vent to anger, 2: stake body, 3: support gland bonnet, 4: outtake tube, 5: air induction conduit, 6: O-ring seal, 7: cuvette snap ring, 8: draw-in groove, 9: air inlet openings, 10: sample stage, 11: fluorescence cuvette, 12: solid sample support; 13: gas outlet, 14: aspiration pump, 15: distributing instrument, 16: detection system.
Embodiment
First, the membrane material of solid fluorescence sample-Han You perylene diimides is pasted on the sample stage 10 of solid sample support 12, again stake body 2 is inserted in fluorescence cuvette 11, the open card of fluorescence cuvette 11 is combined in draw-in groove 8, and by O-ring seal 6, fluorescence cuvette 11 and stake body 2 and cuvette snap ring 7 is sealed tightly.By solid sample support 12 and 11 integral installations of fluorescence cuvette, in detection system 16, air induction conduit 5 is connected to aniline distributing instrument 15, and outtake tube 4 connects aspiration pump 14.Open aspiration pump 14, under suction function, fluorescence cuvette 11 and solid sample support 12 form sealed gas chamber, aniline gas is entered in fluorescence cuvette 11 by air inlet openings 9 by air induction conduit 5, evenly spread to the surface of sample stage 10 Shang perylene diimides, the exciting light that detection system 16 is sent enters and is radiated on sample stage 10 by general fluorescence cuvette 11 1 sides, the fluorescence sending after sample stage 10 Shang perylene diimides are stimulated and exciting light are 90 ° and get back to test macro by fluorescence cuvette 11 opposite sides, perylene diimide carries out the fluorescence sense test under excited state.Test finishes rear aniline gas and enters outtake tube 4 via the opening 1 of giving vent to anger, and is discharged by gas outlet 13.
Do not pass into the irradiation of aniline gas Shi ， perylene diimide stimulated luminescence and send stronger fluorescence; When opening aspiration pump 14, aniline gas is introduced into after fluorescence cuvette 11, there is fluorescent quenching Yu perylene diimide interacts in aniline gas, causes fluorescence intensity to decline to a great extent.The range of decrease of the different ， perylene diimide of the aniline gas concentration fluorescence intensity passing into is also different, realizes the fluorescence sense test of solid fluorescence Yang Pin perylene diimide for aniline gas.
By the use of aspiration pump 14, cuvette snap ring 7 and O-ring seal 6, guarantee in fluorescence cuvette 11 in negative pressure state, when test, do not have Leakage Gas to be measured, guaranteed operating personnel's safety, met the fluorescence sense test of solid fluorescence sample for gas.
Claims (2)
1. for a support for solid fluorescence sample test, it is characterized in that described support comprises: the opening of giving vent to anger (1), stake body (2), support gland bonnet (3), outtake tube (4), air induction conduit (5), O-ring seal (6), cuvette snap ring (7), air inlet openings (9), sample stage (10); The lower end of stake body (2) is provided with sample stage (10), the top of stake body (2) is support gland bonnet (3), cuvette snap ring (7) is embedded in support gland bonnet (3) below through stake body (2), and with support gland bonnet (3) fixing compact, O-ring seal (6) is positioned at the draw-in groove (8) between cuvette snap ring (7) and stake body (2); The air inlet openings (9) of air induction conduit (5) be positioned at sample stage (10) vertical direction directly over, air induction conduit (5) connects support gland bonnet (3) and stake body (2), is coupled to air inlet openings (9); The opening of giving vent to anger (1) of outtake tube (4) is positioned at stake body (2) bottom, outtake tube (4) connects support gland bonnet (3) and stake body (2), is coupled to the opening of giving vent to anger (1).
2. support according to claim 1, is characterized in that the plane of sample stage (10) and the dihedral angle α that surface level forms are greater than 90 °, are less than 180 °.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410373730.6A CN104165875A (en) | 2014-08-01 | 2014-08-01 | Support applied to testing of solid-state fluorescence samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410373730.6A CN104165875A (en) | 2014-08-01 | 2014-08-01 | Support applied to testing of solid-state fluorescence samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104165875A true CN104165875A (en) | 2014-11-26 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410373730.6A Pending CN104165875A (en) | 2014-08-01 | 2014-08-01 | Support applied to testing of solid-state fluorescence samples |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104165875A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106769862A (en) * | 2016-12-14 | 2017-05-31 | 大连大学 | For the device that spectrum experiment is ventilated to solution in cuvette |
| CN107063872A (en) * | 2017-06-02 | 2017-08-18 | 中国工程物理研究院核物理与化学研究所 | It is a kind of to be used for the room temperature mechanical loading unit of metallic beryllium in neutron scattering experiment |
| CN109668867A (en) * | 2019-01-24 | 2019-04-23 | 深圳供电局有限公司 | Gas detection probe |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200953001Y (en) * | 2006-05-30 | 2007-09-26 | 南昌大学 | Solid support for fluorescence measurement |
| CN100476420C (en) * | 1997-12-05 | 2009-04-08 | 罗奇诊断公司 | Improved electrochemical biosensor test strip |
| CN102590090A (en) * | 2012-02-13 | 2012-07-18 | 浙江师范大学 | In situ infrared spectrum pool for studying gas-liquid-solid three-phase boundary |
| CN202869978U (en) * | 2012-09-20 | 2013-04-10 | 北京大学 | Universal type micro solid sample holder for fluorescence spectrophotometer |
| CN103293033A (en) * | 2013-06-28 | 2013-09-11 | 陕西师范大学 | Gas sampling device applicable to fluorescence detection of micro-trace explosive |
-
2014
- 2014-08-01 CN CN201410373730.6A patent/CN104165875A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100476420C (en) * | 1997-12-05 | 2009-04-08 | 罗奇诊断公司 | Improved electrochemical biosensor test strip |
| CN200953001Y (en) * | 2006-05-30 | 2007-09-26 | 南昌大学 | Solid support for fluorescence measurement |
| CN102590090A (en) * | 2012-02-13 | 2012-07-18 | 浙江师范大学 | In situ infrared spectrum pool for studying gas-liquid-solid three-phase boundary |
| CN202869978U (en) * | 2012-09-20 | 2013-04-10 | 北京大学 | Universal type micro solid sample holder for fluorescence spectrophotometer |
| CN103293033A (en) * | 2013-06-28 | 2013-09-11 | 陕西师范大学 | Gas sampling device applicable to fluorescence detection of micro-trace explosive |
Non-Patent Citations (1)
| Title |
|---|
| JIAN YAO ZHENG: "Hydrogen Peroxide Vapor Sensing with Organic Core/Sheath Nanowire Optical Waveguides", 《ADVANCED MATERIALS》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106769862A (en) * | 2016-12-14 | 2017-05-31 | 大连大学 | For the device that spectrum experiment is ventilated to solution in cuvette |
| CN106769862B (en) * | 2016-12-14 | 2019-08-23 | 大连大学 | The device ventilated for spectrum experiment to solution in cuvette |
| CN107063872A (en) * | 2017-06-02 | 2017-08-18 | 中国工程物理研究院核物理与化学研究所 | It is a kind of to be used for the room temperature mechanical loading unit of metallic beryllium in neutron scattering experiment |
| CN109668867A (en) * | 2019-01-24 | 2019-04-23 | 深圳供电局有限公司 | Gas detection probe |
| CN109668867B (en) * | 2019-01-24 | 2024-02-06 | 深圳供电局有限公司 | Gas detection probe |
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| PB01 | Publication | ||
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| WD01 | Invention patent application deemed withdrawn after publication | ||
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Application publication date: 20141126 |