CN202471712U - Test strip for chloramphenicol detection - Google Patents
Test strip for chloramphenicol detection Download PDFInfo
- Publication number
- CN202471712U CN202471712U CN2012200721389U CN201220072138U CN202471712U CN 202471712 U CN202471712 U CN 202471712U CN 2012200721389 U CN2012200721389 U CN 2012200721389U CN 201220072138 U CN201220072138 U CN 201220072138U CN 202471712 U CN202471712 U CN 202471712U
- Authority
- CN
- China
- Prior art keywords
- spun glass
- carrier
- glass fibres
- nitrocellulose membrane
- chloramphenicol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
The utility model relates to a test strip for a chloramphenicol detection, is effectively used for solving problems of complex detection operation, high cost and inconvenience due to the residue of chloramphenicol, wherein water diversion glass fibres, carrier glass fibres, a nitrocellulose membrane and a water-absorbent cotton pulp plate are sequentially arranged on a base material PVC (polyvinyl chloride) plate; covering membranes are arranged on the water diversion glass fibres, the carrier glass fibres and the water-absorbent cotton pulp plate; an anti-chloramphenicol protein conjugate monoclonal antibody colloidal gold labelling substances are arranged on the carrier glass fibres; a detection line enveloped with a chloramphenicol protein conjugate and a quality control line enveloped with an anti-rat immune globulin antibody are arranged on the nitrocellulose membrane; the lower end of the nitrocellulose membrane is connected with the upper ends of the carrier glass fibres; the upper end of the nitrocellulose membrane is connected with the lower end of the water-absorbent cotton pulp plate; and the lower ends of the carrier glass fibres are connected with the upper ends of the water diversion glass fibres. The test strip disclosed by the utility model can be effectively used for detecting the content of chloramphenicol in a food, as well as is simple in structure, convenient and fast in use, and accurate in result.
Description
Technical field
The utility model relates to the detection articles for use, and particularly a kind of chloromycetin detects test-strips.
Background technology
(Chloramphenicol is a kind of broad-spectrum antibiotic CAP) to chloromycetin, has good antibiotic pharmacological property, is widely used in the control of various infectious diseases in all kinds of poultry, domestic animal, aquatic animal (fish, shrimp etc.) and the honeybee.But its residual serious threat health in food such as animal flesh, milk, egg can cause diseases such as alpastic anemia and granular white blood cells deficiency disease, and the medicament residue of low concentration also can bring out the drug resistance of pathogenic bacteria.
At present, mostly the detection mode of residual chloromycetin is that microorganism suppresses method, physical-chemical process (comprising high performance liquid chromatography, TLC, vapor-phase chromatography) and immunological detection (ELISA and radiommunoassay) etc. in the food.Yet; These detection modes need long experimental period and corresponding expensive experimental equipment; Be limited in using in the laboratory; Very inconvenient, Along with people's presses for a kind of test-strips that can easily and fast, stably detect residual chloromycetin to the pay attention to day by day of food security and the rapid growth of foreign trade.
Summary of the invention
To above-mentioned situation, for overcoming the prior art defective, the purpose of the utility model just provides a kind of chloromycetin and detects test-strips, and the detecting operation that can effectively solve residual chloromycetin is complicated, and cost is high, inconvenient problem.
The technical scheme that the utility model solves is; Comprise base material PVC plate and overlay film; Base material PVC plate upper surface is bottom-up to have diversion spun glass, carrier spun glass, nitrocellulose membrane and absorbent wool pulpboard successively; Diversion spun glass, carrier spun glass and be coated with overlay film above the absorbent wool pulpboard, the carrier spun glass upper surface of overlay film below is adsorbed with chloramphenicol resistance protein conjugate monoclonal antibody colloid gold label thing, and the nitrocellulose membrane upper surface is distributed with detection line that encapsulates the chloromycetin protein conjugate and the nature controlling line that encapsulates anti-rat immune globulin antibody from bottom to top (wherein; Lower and upper diversion direction and the suction direction of being meant respectively from bottom to top; The diversion spun glass is the lower end of the utility model, i.e. diversion direction, and the absorbent wool pulpboard is the upper end; Direction promptly absorbs water); The upper end of the lower end of nitrocellulose membrane and carrier spun glass closely links together, and the lower end of the upper end of nitrocellulose membrane and absorbent wool pulpboard closely links together, and the upper end of the lower end of carrier spun glass and diversion spun glass closely links together.
The utility model can be effective to detect chloromycetin content in the food, and simple in structure, easy to use, quick, the result is accurate.
Description of drawings
Fig. 1 is the structural perspective of the utility model.
Embodiment
Elaborate below in conjunction with the embodiment of accompanying drawing to the utility model.
Provide by Fig. 1; The utility model comprises base material PVC plate and overlay film; Base material PVC plate 1 upper surface is bottom-up to have diversion spun glass 3, carrier spun glass 4, nitrocellulose membrane 2 and absorbent wool pulpboard 5 successively; Diversion spun glass, carrier spun glass and be coated with overlay film above the absorbent wool pulpboard; The carrier spun glass upper surface of overlay film below is adsorbed with chloramphenicol resistance protein conjugate monoclonal antibody colloid gold label thing 10; The nitrocellulose membrane upper surface is distributed with detection line 8 that encapsulates the chloromycetin protein conjugate and the nature controlling line 9 that encapsulates anti-rat immune globulin antibody from bottom to top; The upper end of the lower end of nitrocellulose membrane and carrier spun glass closely links together, and the lower end of the upper end of nitrocellulose membrane and absorbent wool pulpboard closely links together, and the upper end of the lower end of carrier spun glass and diversion spun glass closely links together.
Described overlay film is made up of bottom coverlay 7 and top coverlay 6, and the bottom coverlay covers the upper surface of diversion spun glass and carrier spun glass, and the top coverlay covers the upper surface of absorbent wool pulpboard; Described overlay film is a pressure sensitive membrane.
It is to be noted; The utility model creative contribution is; With prior art through scientific and reasonable combination and configuration; Constitute novel chloromycetin and detect test-strips, be effective to detect chloromycetin in the food, the technical scheme that all that adopt structurally that equivalent replacement means have done and the utility model do not have essential distinction in essence all belongs to the protection domain of the utility model.
The utility model test-strips in use; Vertically insert diversion spun glass one end in the specimen samples; Sample under capillary action, along test-strips to the swimming of absorbent wool pulpboard direction, if contain chloromycetin in the specimen samples; To form labelled antibody-antigenic compound with the reaction of chloramphenicol resistance protein conjugate monoclonal antibody colloid gold label thing; When the specimen samples swimming does not then react when being coated with chloromycetin protein conjugate detection line, and with the nature controlling line reaction, a red line (nature controlling line) only appears in the result; If there is not chloromycetin in the specimen samples, chloromycetin protein conjugate and nature controlling line combine to form the red line band on chloramphenicol resistance protein conjugate monoclonal antibody colloid gold label thing and the coated film, and two red lines appear in the result; Red line do not occur like nature controlling line, show that then test-strips lost efficacy.Therefore, utilize this test-strips, only needed to detect chloromycetin in 5～10 minutes.This sensitivity simple in structure, easy to operate, quick, result are accurate, do not need instrument and equipment, promptly can detect a large amount of samples simultaneously.Be applicable to units such as some animal-breeding fields, food processing enterprises and food import and export inspection and quarantine.
The utility model test; Utilizing 60 parts of this test-strips and enzyme-linked immunosorbent assay kit blank determinations (positive 20 parts, negative 40 parts) milk sample, is to judge limit value with 1ng/ml; False positive and false negative do not occur, testing result conforms to ELISA method result fully.
Claims (3)
1. a chloromycetin detects test-strips; Comprise base material PVC plate and overlay film; It is characterized in that; Base material PVC plate (1) upper surface is bottom-up to have diversion spun glass (3), carrier spun glass (4), nitrocellulose membrane (2) and absorbent wool pulpboard (5) successively; Diversion spun glass, carrier spun glass and be coated with overlay film above the absorbent wool pulpboard; The carrier spun glass upper surface of overlay film below is adsorbed with chloramphenicol resistance protein conjugate monoclonal antibody colloid gold label thing (10), and the nitrocellulose membrane upper surface is distributed with detection line (8) that encapsulates the chloromycetin protein conjugate and the nature controlling line (9) that encapsulates anti-rat immune globulin antibody from bottom to top, and the upper end of the lower end of nitrocellulose membrane and carrier spun glass closely links together; The lower end of the upper end of nitrocellulose membrane and absorbent wool pulpboard closely links together, and the upper end of the lower end of carrier spun glass and diversion spun glass closely links together.
2. chloromycetin according to claim 1 detects test-strips; It is characterized in that; Described overlay film is made up of bottom coverlay (7) and top coverlay (6); The bottom coverlay covers the upper surface of diversion spun glass and carrier spun glass, and the top coverlay covers the upper surface of absorbent wool pulpboard.
3. chloromycetin according to claim 1 detects test-strips, it is characterized in that described overlay film is a pressure sensitive membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012200721389U CN202471712U (en) | 2012-03-01 | 2012-03-01 | Test strip for chloramphenicol detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012200721389U CN202471712U (en) | 2012-03-01 | 2012-03-01 | Test strip for chloramphenicol detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202471712U true CN202471712U (en) | 2012-10-03 |
Family
ID=46919864
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012200721389U Expired - Fee Related CN202471712U (en) | 2012-03-01 | 2012-03-01 | Test strip for chloramphenicol detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202471712U (en) |
-
2012
- 2012-03-01 CN CN2012200721389U patent/CN202471712U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121003 Termination date: 20140301 |