CN202415566U - Filtering device for microcapsules and microcarriers - Google Patents
Filtering device for microcapsules and microcarriers Download PDFInfo
- Publication number
- CN202415566U CN202415566U CN2012200090413U CN201220009041U CN202415566U CN 202415566 U CN202415566 U CN 202415566U CN 2012200090413 U CN2012200090413 U CN 2012200090413U CN 201220009041 U CN201220009041 U CN 201220009041U CN 202415566 U CN202415566 U CN 202415566U
- Authority
- CN
- China
- Prior art keywords
- stay pipe
- microcarrier
- microcapsule
- support tube
- filtration unit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model discloses a filtering device for microcapsules and microcarriers, which comprises an inner support tube (103) and an outer support tube (101) which are made of stainless steel and concentrically sleeved, wherein the outer support tube (101) is longer than the inner support tube (103), the periphery of the outer wall of the outer support tube (101) is provided with three handles (102) at equal intervals, an orifice at the inner end of the inner support tube (103) is provided with a nylon or metal filtering screen (104). The filtering device for three-dimensional cell cultivation has the following beneficial effects: cell cultivation liquid can be filtered out freely, but microcapsules and microcarriers cannot be filtered out; and the filtering device can be arranged on a cultivation plate or a centrifuge tube to collect liquid conveniently due to the arrangement of the three handles. The filtering device can be used for quickly separating microcapsules or microcarriers from the cultivation liquid and has the advantages of simple structure, low manufacturing cost, easiness in manufacture and operation and easiness in popularization and application.
Description
Technical field
The present invention relates to a kind of filtration unit, more particularly, relate to cell three-dimensional and cultivate filtration unit with microcapsule and microcarrier.
Background technology
The microcarrier cell culture method is a kind of large scale culturing technology that is used to cultivate the anchorage dependence cell.This culture technique is the particle (microcarrier) that in bio-reactor, adds the materials for support of nutrient solution and a kind of pair cell toxicological harmless effect, makes cell in microcarrier surface attachment and growth, and makes microcarrier keep suspended state through continuous stirring.A large amount of microcarriers is that cell provides great attaching surface in the nutrient solution, thereby can realize the high-density culture of cell.Its main points of microencapsulation culture technique are: under aseptic condition, will intend cultured cells, biologically active substance and growth medium and be wrapped in jointly in the thin semi-permeable membranes and form micro-capsule, and again micro-capsule put into culture systems and cultivate.Utilize microcapsule and microcarrier to cultivate three-dimensional cell, in organizational project, be used widely in aspects such as Transplanted cells, drug screening.In the research of microcapsule or microcarrier culturing cell, often need investigate different cytokines, external medicine and the metabolic influence of other factor cell growth, this just relates to the replacing cell culture fluid, operations such as sampling.Usually adopt microcapsule or microcarrier natural subsidence behind culture plate or culturing bottle bottom, sucking-off/inclining the old nutrient solution in upper strata, adds fresh medium, perhaps directly collects microcarrier or microencapsulated cell.There is following problem in this operation: 1. often cause the loss of microcapsule or microcarrier; 2. it is not thorough to change liquid, and residual old nutrient solution causes the new nutrient solution add-on of pastille etc. different relatively, and experimental error is big; 3. the running time long, be unfavorable for rapid detection.
Summary of the invention
The present invention provides a kind of simple in structure in order to solve existing in prior technology the problems referred to above, makes and the microcarrier of processing ease or the filtration unit of microcapsule culturing cell.
For realizing above-mentioned purpose, a kind of microcapsule of the present invention and microcarrier filtration unit comprise stay pipe and outer stay pipe in the inside and outside stainless steel of belled with one heart; The length of said outer stay pipe is longer than said interior stay pipe.On the said outer stay pipe outer wall circumference three handles are set uniformly-spaced; The medial extremity mouth of pipe place of stay pipe is provided with nylon or metal filter made net in said.
Microcapsule of the present invention and microcarrier filtration unit further improve being, are provided with the micron order running fit between said interior stay pipe and the outer stay pipe.Under a kind of optimal way, said outer stay pipe internal diameter is 10mm～30mm, highly is 15mm～25mm; The stay pipe height is 5mm～8mm in said; Said filtering net is selected 100 orders～200 orders for use; Said decreased food is selected 25mm～30mm for use.
A kind of microcapsule and microcarrier filtration unit comprise outer stay pipe, support three handles on the tube wall outward, interior stay pipe and filtering net.Use 100～200 order filtering nets, cell culture fluid can freely leach, and microcarrier and microcapsule can not pass through filter screen; Equidistant three handles make this device directly frame on culture plate or centrifuge tube, be convenient to collect liquid; Outer stay pipe, outward support on the tube wall three handles and in stay pipe all adopt stainless steel, filtering net is nylon wire or wire netting, whole device can autoclaving.
Advantage of the present invention and positively effect are:
1. can realize the sharp separation of microcapsule or microcarrier and nutrient solution;
2. simple in structure, cheap, be easy to processing and popularization.
Cell three-dimensional of the present invention is cultivated with microcapsule and microcarrier filtration unit, simple in structure, making and processing ease; It is more convenient to operate, feasible to use this device to make changing liquid, sampling, dosing etc. in microencapsulated cell and the microcarrier cell cultures.
Description of drawings
Fig. 1 is the plan structure synoptic diagram of microcapsule of the present invention and microcarrier filtration unit;
Fig. 2 be microcapsule of the present invention and microcarrier filtration unit partly cut open structural representation;
Fig. 3 is the user mode synoptic diagram that microcapsule of the present invention and microcarrier filtration unit are used in combination with six orifice plates;
Among the figure: the outer stay pipe of 101-; 102-supports three handles on the tube wall outward; Stay pipe in the 103-; The 104-filtering net; 301-microcapsule and microcarrier filtration unit; 302-six orifice plates.
Embodiment
As illustrated in fig. 1 and 2, microcapsule of the present invention and microcarrier filtration unit comprise stay pipe 103 and outer stay pipe 101 in the inside and outside stainless steel of belled with one heart.Among the figure, the length of outer stay pipe 101 is longer than interior stay pipe 103, and under the optimal way, outer stay pipe length is 15mm～25mm, and interior stay pipe length is 5mm～8mm; In addition, outer stay pipe 101 internal diameters are 10mm～30mm.Be provided with the micron order running fit between interior stay pipe 103 and the outer stay pipe 101.
Among Fig. 1, the medial extremity mouth of pipe place of interior stay pipe 103 is provided with nylon or metal filter made net 104.Filtering net 104 is 100 orders～200 orders.In addition, on the outer stay pipe 101 outer wall circumference three handles 102 are set uniformly-spaced, its length is 25mm～30mm.
The state that microcapsule of the present invention as shown in Figure 3 and microcarrier filtration unit are used in combination with six orifice plates.Get apparatus of the present invention 301 and be placed on the six new orifice plates 302, draw whole cultures on filtration unit 301, the liquid filter down; Microcarrier or microcapsule are trapped within on the filter screen, and with fresh nutrient solution drip washing filter screen several times, the left-hand thread strainer is on orifice plate for use; With fresh nutrient solution recoil filter screen; Whole microcarriers or microcapsule just are flushed in the hole, continue to cultivate, thereby accomplish the whole liquid process of changing.If want to get microcarrier or microcapsule, can from filter screen, directly take away.
The above; Be merely the preferable embodiment of the present invention; But protection scope of the present invention is not limited thereto; Any technician who is familiar with the present technique field is equal to replacement or change according to technical scheme of the present invention and inventive concept thereof in the technical scope that the present invention discloses, all should be encompassed within protection scope of the present invention.
Claims (5)
1. microcapsule and microcarrier filtration unit is characterized in that: comprise stay pipe (103) and outer stay pipe (101) in the inside and outside stainless steel of belled with one heart; The length of said outer stay pipe (101) is longer than said interior stay pipe (103);
On said outer stay pipe (101) the outer wall circumference three handles (102) are set uniformly-spaced; The medial extremity mouth of pipe place of stay pipe (103) is provided with nylon or metal filter made net (104) in said.
2. according to said microcapsule of claim 1 and microcarrier filtration unit, it is characterized in that: be provided with the micron order running fit between stay pipe (103) and the outer stay pipe (101) in said.
3. according to said microcapsule of claim 2 and microcarrier filtration unit, it is characterized in that: said outer stay pipe (101) internal diameter is 10mm～30mm, highly is 15mm～25mm; Stay pipe (103) highly is 5mm～8mm in said.
4. according to arbitrary said microcapsule of claim 1-3 and microcarrier filtration unit, it is characterized in that: said filtering net (104) is 100 orders～200 orders.
5. according to said microcapsule of claim 4 and microcarrier filtration unit, it is characterized in that: said handle (102) length is 25mm～30mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012200090413U CN202415566U (en) | 2012-01-10 | 2012-01-10 | Filtering device for microcapsules and microcarriers |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012200090413U CN202415566U (en) | 2012-01-10 | 2012-01-10 | Filtering device for microcapsules and microcarriers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202415566U true CN202415566U (en) | 2012-09-05 |
Family
ID=46740346
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012200090413U Expired - Fee Related CN202415566U (en) | 2012-01-10 | 2012-01-10 | Filtering device for microcapsules and microcarriers |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202415566U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468568A (en) * | 2013-09-16 | 2013-12-25 | 益善生物技术股份有限公司 | Filtering device, filtering piece and filtering method |
| CN106635769A (en) * | 2016-11-07 | 2017-05-10 | 深圳市达科为生物工程有限公司 | Device and method for separating cells |
| CN107849507A (en) * | 2015-05-29 | 2018-03-27 | 迈索布拉斯特国际有限公司 | Method and apparatus for cell to be separated with microcarrier |
-
2012
- 2012-01-10 CN CN2012200090413U patent/CN202415566U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468568A (en) * | 2013-09-16 | 2013-12-25 | 益善生物技术股份有限公司 | Filtering device, filtering piece and filtering method |
| CN107849507A (en) * | 2015-05-29 | 2018-03-27 | 迈索布拉斯特国际有限公司 | Method and apparatus for cell to be separated with microcarrier |
| US11242506B2 (en) | 2015-05-29 | 2022-02-08 | Mesoblast International Sárl | Methods and apparatus for separating cells from microcarriers |
| CN107849507B (en) * | 2015-05-29 | 2022-06-28 | 迈索布拉斯特国际有限公司 | Method and apparatus for separating cells from microcarriers |
| CN106635769A (en) * | 2016-11-07 | 2017-05-10 | 深圳市达科为生物工程有限公司 | Device and method for separating cells |
| CN106635769B (en) * | 2016-11-07 | 2019-06-28 | 深圳市达科为生物工程有限公司 | Cell separation apparatus and cell isolation method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5270207A (en) | Circulatory culture equipment | |
| CN201933090U (en) | Interception device for perfusion culture of cell microcarrier | |
| JP2014158461A (en) | Large scale cell harvesting method for pack-bed culture device | |
| US9101857B2 (en) | Gas scrubbed perfusion filter | |
| KR20210022162A (en) | Continuously controlled hollow fiber bioreactor | |
| CN202415566U (en) | Filtering device for microcapsules and microcarriers | |
| CN112457985A (en) | Perfusion culture chip and perfusion system | |
| US20240263125A1 (en) | Adherent Cell Culture Substrate Sampling Device | |
| CN107703261B (en) | A kind of experimental simulation device and experimental method of microorganism and deposit interaction | |
| CN205347445U (en) | Cell culture fibre membrane support frame, cell culture support and cell culture device | |
| JPWO2016140213A1 (en) | Cell culture method using hollow fiber module | |
| US20230174913A1 (en) | Novel high-density microcarrier retention device for perfusion culture and method of use thereof | |
| CN201924018U (en) | Cell filtration device | |
| CN210287378U (en) | High-affinity cell culture dish | |
| CN205115496U (en) | Microcapsule quick separation device | |
| CN204466635U (en) | Device for mass propagation of entomopathogenic nematodes in living bodies of laboratories | |
| CN201737948U (en) | Perfusion biological culture device | |
| JP7009957B2 (en) | Cell culture container and cell culture device | |
| CN201343538Y (en) | Liquid removing device for culturing microcarrier cells | |
| CN204860463U (en) | Can be used to add in succession tissue culture bottle of nutrient solution | |
| CN211302243U (en) | Adjustable filtration auxiliary device | |
| CN214572017U (en) | Perfusion culture chip and perfusion system | |
| JP2015167550A (en) | Cell cultivation device | |
| CN102367413B (en) | Quantitation concentrator of noctiluca scientillans living cells | |
| CN202148312U (en) | Novel alga separation culturing device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120905 Termination date: 20130110 |