CN102367413B - Quantitation concentrator of noctiluca scientillans living cells - Google Patents
Quantitation concentrator of noctiluca scientillans living cells Download PDFInfo
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- CN102367413B CN102367413B CN 201110325704 CN201110325704A CN102367413B CN 102367413 B CN102367413 B CN 102367413B CN 201110325704 CN201110325704 CN 201110325704 CN 201110325704 A CN201110325704 A CN 201110325704A CN 102367413 B CN102367413 B CN 102367413B
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- noctiluca scientillans
- surge drum
- cylinder
- noctiluca
- scientillans
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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Abstract
The invention discloses a quantitation concentrator of noctiluca scientillans living cells. The quantitation concentrator comprises a quantitating cylinder with a bottom and a collecting cylinder without a bottom. A positioning sleeve is arranged in the quantitating cylinder. The positioning sleeve is provided with fixing feet fixedly connected to the bottom of the quantitating cylinder. The collecting cylinder is movably connected to the positioning sleeve. Sieving gauze with apertures of 80 to 180 micrometers is arranged between the collecting cylinder and the positioning sleeve. The cylinder port of the quantitating cylinder is higher than the upper rim of the positioning sleeve, and is lower than the port of the collecting cylinder. Therefore, a quantitated noctiluca scientillans living cell water sample is slowly poured into the collecting cylinder; most of the water content in the noctiluca scientillans living cell water sample flows into the quantitating cylinder and is over-flown from the cylinder port; noctiluca scientillans living cells are separated by the sieving gauze, and are kept in the collecting cylinder, such that the noctiluca scientillans living cells are concentrated. The density of the noctiluca scientillans living cells is high, and a uniformly distributed suspension is formed. Collapse and declination of the living cells are rarely occurred. Therefore, the quantitative concentrator provided by the invention is beneficial for the quantitative calculation of noctiluca scientillans living cells, and for the researches of crucial information substances in noctiluca scientillans living cell division, noctiluca scientillans cell life history, and noctiluca scientillans population proliferation.
Description
Technical field
The present invention relates to the algae concentrating unit, be specifically related to a kind of quantitative thickener of Noctiluca scientillans viable cell.
Background technology
Noctiluca scientillans (Noctiluca scintillans or N. miliaris) has another name called noctiluca, is a kind of unicellular study on planktonic dinoflagellates kind of heterotrophism, and it is spherical that vegetative cell is generally, and diameter is at 200 μ m to 2000 μ m.Noctiluca scientillans is a kind of harmful study on planktonic dinoflagellates kind of normal red tide, in worldwide the marine site and breeding environment, red tide report is arranged, Noctiluca scientillans red tide key issue progress is always relatively slow, and an important reason is that the research such as key message material and mechanism of action quite lacks in relevant Noctiluca scientillans cell fission, cell life history and meaning thereof, the population augment process.The monitoring method that adopts at present usually is that the Noctiluca scientillans density in the fixing water sample is recorded and screens, workload is large like this, be difficult to obtain the Noctiluca scientillans cell of capacity, directly have influence on open-air and indoor research to Noctiluca scientillans, being subject to greatly to the research of Noctiluca scientillans viable cell, research limits.Under field conditions (factors), a large amount of often obtaining of Noctiluca scientillans cell only can be realized when the high-density aggregation state, gather difficulty, namely allow to gather also missed the research aircraft meeting of assembling front cell in-situ feature.In desk research, the writing task amount is large one by one to Noctiluca scientillans in whole water samples of culture dish, efficient is low, and the floating feature of and Noctiluca scientillans low owing to density in the water sample, make water sample be difficult to form even distribution, key message material etc. is made troubles in research Noctiluca scientillans cell fission, cell life history and the population augment process.The blue-green algae concentrating unit is disclosed although publication number is the application for a patent for invention of CN102102083, in tank body, establish air supporting cylinder, surge drum and whipping appts, it allows blue-green algae float by the air supporting principle, stir with the blue-green algae of whipping appts with come-up, enter surge drum and discharged by water shoot, but the purpose of this device, it is destructive remove portion blue-green algae, alleviate compressor load, can not be applied to the concentrated research of algae metering, more can't concentrate the little algae-unicellular algae that lives with quantitative.Present also unexposed technology and the device that concentrated and quantitative Noctiluca scientillans is arranged.
Summary of the invention
Technical problem to be solved by this invention provides a kind of concentrating function that namely has, again can be quantitative, and few to the damage of Noctiluca scientillans viable cell, study the easily quantitative thickener of Noctiluca scientillans viable cell.
The present invention solves the problems of the technologies described above the technical scheme that adopts: a kind of quantitative thickener of Noctiluca scientillans viable cell, this quantitative thickener includes the quantitation tube at the end and bottomless surge drum, be provided with locating sleeve in the described quantitation tube, described locating sleeve is provided with the fixing feet that is fixedly connected with the bottom of described quantitation tube, described surge drum and described locating sleeve are flexibly connected, be provided with the mesh aperture between described surge drum and the described locating sleeve and be 80-180 microns bolting silk, the nozzle of described quantitation tube is higher than the upper limb of described locating sleeve, is lower than the nozzle of described surge drum.
Described surge drum and described locating sleeve movable connection method are that described surge drum is screwed onto in the described locating sleeve.
Described surge drum and described locating sleeve movable connection method are that described surge drum is plugged in the described locating sleeve.
Described surge drum and described locating sleeve movable connection method are that described surge drum is socketed in outside the described locating sleeve.
Described surge drum and described locating sleeve movable connection method are that described surge drum is screwed onto outside the described locating sleeve.
Compared with prior art, the invention has the advantages that a kind of quantitative thickener of Noctiluca scientillans viable cell, this quantitative thickener includes the quantitation tube at the end and bottomless surge drum, be provided with locating sleeve in the quantitation tube, locating sleeve is provided with the fixing feet that is fixedly connected with the bottom of quantitation tube, surge drum and locating sleeve are flexibly connected, and are provided with the mesh aperture between surge drum and the locating sleeve and are 80-180 microns bolting silk, and the nozzle of quantitation tube is higher than the upper limb of locating sleeve, is lower than the nozzle of surge drum; Quantitative like this Noctiluca scientillans viable cell water sample is slowly at the uniform velocity poured in the surge drum, most of moisture content of Noctiluca scientillans viable cell water sample flows to quantitation tube and overflows from nozzle, the Noctiluca scientillans viable cell is but isolated by bolting silk, stay in the surge drum and concentrated, density is higher, the Noctiluca scientillans viable cell is highly concentrated in the surge drum water sample, the Noctiluca scientillans viable cell density is high in the surge drum, form equally distributed suspended state, and the viable cell shrinkage decays seldom, be conducive to the Noctiluca scientillans viable cell quantitative counting in the surge drum, Noctiluca scientillans cell fission, the research such as key message material in cell life history and the population augment process.This apparatus structure is simple, and is easy to use, and Noctiluca scientillans viable cell damage is few, so the present invention is a kind of concentrating function that namely has, again can be quantitative, and few to the damage of Noctiluca scientillans viable cell, study the easily quantitative thickener of Noctiluca scientillans viable cell.
Description of drawings
Fig. 1 is the structure cut-away view of embodiment 1;
Fig. 2 is the structure cut-away view of embodiment 3.
Embodiment
Embodiment is described in further detail the present invention below in conjunction with accompanying drawing.
Embodiment 1
The quantitative thickener of a kind of Noctiluca scientillans viable cell as shown in Figure 1, this quantitative thickener includes the quantitation tube 1 at the end and bottomless surge drum 2, be provided with locating sleeve 3 in the quantitation tube 1, locating sleeve 3 is provided with the fixing feet 4 that is fixedly connected with the bottom of quantitation tube 1, it is 80-180 microns bolting silk 5 that locating sleeve 3 is coated with the mesh aperture, surge drum 2 is plugged in the locating sleeve 3, bolting silk 5 is just at 2 times interruption-formings of surge drum filtering net firmly like this, the nozzle 11 of quantitation tube 1 is higher than the upper limb 31 of locating sleeve 3, be lower than the nozzle 21 of surge drum 2, the height of the nozzle 11 of quantitation tube 1 can be decided according to concentrated desirability, as being lower than 1/ 2 tube port positions of surge drum 2,1/ 3 tube port positions, 2/ 5 tube port positions etc.; Using method be with the Noctiluca scientillans water sample of quantitative collection or or cultivate water body and slowly at the uniform velocity pour in the surge drum, it is 80,100,120,130,150 or 180 microns etc. silk cover filtering by the mesh aperture, the Noctiluca scientillans viable cell is still stayed in the surge drum, most water flows into quantitation tube, and overflow from the quantitation tube nozzle, Noctiluca scientillans viable cell density in the surge drum is higher, as required, can further draw moisture content in the quantitation tube, make the interior Noctiluca scientillans viable cell of surge drum further concentrated, density is higher.Concentrated Noctiluca scientillans viable cell forms equally distributed suspended state, damage is few, just can draw quantitative concentrated solution or directly move on to the microscopically quantitative counting and a large amount of concentrated viable cell are carried out observational study, such as the researchs such as key message material, original position feature in Noctiluca scientillans cell fission, cell life history and the population augment process.When this device used, the Noctiluca scientillans cell is kept suspended state all the time without doing, and lacks damage, and was easy to use.Take out surge drum after using, change bolting silk and just can continue to use, can the Noctiluca scientillans viable cell of natural water samples or indoor cultivation be concentrated at any time.
Substantially the same manner as Example 1, difference is that surge drum 2 is provided with outside screw, and locating sleeve 3 is provided with internal thread, and surge drum 2 is to be screwed onto in the locating sleeve 3.
As shown in Figure 2, substantially the same manner as Example 1, difference is that surge drum 2 is provided with internal thread, and locating sleeve 3 is provided with outside screw, and surge drum 2 is to be screwed onto outside the locating sleeve 3.
Substantially the same manner as Example 1, difference is that surge drum 2 is socketed in outside the locating sleeve 3.
Claims (5)
1. the quantitative thickener of a Noctiluca scientillans viable cell, it is characterized in that this quantitative thickener includes the quantitation tube at the end and bottomless surge drum, be provided with locating sleeve in the described quantitation tube, described locating sleeve is provided with the fixing feet that is fixedly connected with the bottom of described quantitation tube, described surge drum and described locating sleeve are flexibly connected, be provided with the mesh aperture between described surge drum and the described locating sleeve and be 80-180 microns bolting silk, the nozzle of described quantitation tube is higher than the upper limb of described locating sleeve, is lower than the nozzle of described surge drum.
2. the quantitative thickener of a kind of Noctiluca scientillans viable cell as claimed in claim 1 is characterized in that described surge drum and described locating sleeve movable connection method are that described surge drum is screwed onto in the described locating sleeve.
3. the quantitative thickener of a kind of Noctiluca scientillans viable cell as claimed in claim 1 is characterized in that described surge drum and described locating sleeve movable connection method are that described surge drum is plugged in the described locating sleeve.
4. the quantitative thickener of a kind of Noctiluca scientillans viable cell as claimed in claim 1 is characterized in that described surge drum and described locating sleeve movable connection method are that described surge drum is socketed in outside the described locating sleeve.
5. the quantitative thickener of a kind of Noctiluca scientillans viable cell as claimed in claim 1 is characterized in that described surge drum and described locating sleeve movable connection method are that described surge drum is screwed onto outside the described locating sleeve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110325704 CN102367413B (en) | 2011-10-24 | 2011-10-24 | Quantitation concentrator of noctiluca scientillans living cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110325704 CN102367413B (en) | 2011-10-24 | 2011-10-24 | Quantitation concentrator of noctiluca scientillans living cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102367413A CN102367413A (en) | 2012-03-07 |
| CN102367413B true CN102367413B (en) | 2013-04-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110325704 Expired - Fee Related CN102367413B (en) | 2011-10-24 | 2011-10-24 | Quantitation concentrator of noctiluca scientillans living cells |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114874913B (en) * | 2022-05-12 | 2023-02-07 | 江苏海洋大学 | Method for manufacturing luminous algae fluorescent ecological bottle |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2237944Y (en) * | 1994-11-02 | 1996-10-23 | 山东泰山酿酒饮料(集团)股份有限公司 | Device for extracting seaweed juice |
| CN102102083A (en) * | 2010-01-26 | 2011-06-22 | 南京清波蓝藻环保科技有限公司 | Blue-green alga concentrating tank |
-
2011
- 2011-10-24 CN CN 201110325704 patent/CN102367413B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2237944Y (en) * | 1994-11-02 | 1996-10-23 | 山东泰山酿酒饮料(集团)股份有限公司 | Device for extracting seaweed juice |
| CN102102083A (en) * | 2010-01-26 | 2011-06-22 | 南京清波蓝藻环保科技有限公司 | Blue-green alga concentrating tank |
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| Publication number | Publication date |
|---|---|
| CN102367413A (en) | 2012-03-07 |
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Granted publication date: 20130424 Termination date: 20191024 |