CN202265565U - Single outflow rumen in-vitro continuous culture system fermentation device - Google Patents

Single outflow rumen in-vitro continuous culture system fermentation device Download PDF

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Publication number
CN202265565U
CN202265565U CN 201120231275 CN201120231275U CN202265565U CN 202265565 U CN202265565 U CN 202265565U CN 201120231275 CN201120231275 CN 201120231275 CN 201120231275 U CN201120231275 U CN 201120231275U CN 202265565 U CN202265565 U CN 202265565U
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China
Prior art keywords
fermentation
stirring
filter screen
rumen
connecting rod
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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CN 201120231275
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Chinese (zh)
Inventor
杨连玉
胡晓丽
刘庆福
李喜武
孟宪章
卢广林
车东升
陈晓霞
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Jilin Agricultural University
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Jilin Agricultural University
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Priority to CN 201120231275 priority Critical patent/CN202265565U/en
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Publication of CN202265565U publication Critical patent/CN202265565U/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means

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  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model relates to a single outflow rumen in-vitro continuous culture system fermentation device, and belongs to the field of bionic machinery. A fermentation cover of a fermentation tank is fixedly connected with a feeding device through a hollow connection shaft; the tank body and the fermentation cover are fixedly and sealedly connected through a screw, a nut and a sealing ring; a stirrer connecting rod of a stirring device 2 is rotatably connected with the fermentation cover through a stirring shaft sleeve and a stirring screw cap; a stirring impeller and a stirring sail are welded on the stirrer connecting rod; a bottom of a cylindrical filtering device and an annular groove at the tank body bottom are clamped and fixed together; a filter screen is fixed on a filter screen support by a filter screen inner sleeve and a filter screen outer sleeve. The advantages are that a single outflow rumen in-vitro continuous culture fermentation process is easy to control, and convenient for operation; and the sealing is good; the generated chyme and gas are easy to be separated and collected, which ensures that the simulation process can be completed smoothly.

Description

Single cud in-vitro cultivation system fermentation unit that outflows
Technical field
The utility model belongs to the design of simulation bionic mechanical field, especially fermentation unit, is the ideal model of realizing the simulation rumen fermentation.
Background technology
Along with people's is to the attention day by day of ruminant tumor gastric research, and the device outside design of research rumen zymosis more and more trends towards the simulation to the rumen zymosis truth.Therefore, various in vitro artificial rumen methods are arisen at the historic moment.In vitro method is divided into batch culture method and continuous culture method by its fermenting characteristic.Batch culture method, i.e. the disposable adding of microbial inoculant thing and fermentation substrate after certain hour is cultivated, finishes to cultivate in the set time.Owing to degradation reason under product inhibition, the pH, the vigor that batch cultivation has mikrobe descends, forms problem appearance such as variation; And the present invention has overcome this shortcoming, has realized the continuous discharge of tunning in the vitro culture system.Because These characteristics, make in-vitro cultivation method environment in the simulation rumen more accurately, for the digestion of quantitative examination cud provides a comparatively ideal model.
Summary of the invention
The utility model provides a kind of simulation bionic mechanical device, to solve the mechanize of present single outflow rumen zymosis device, the simulation that the environment fermenting process becomes in the cud is become a reality.The technical scheme that the utility model is taked is: the fermentation of fermentor tank 1 lid 101 is fixedly connected through hollow coupling shaft with drawing-in device 4; Tank body 105 covers 101 through screw 102, nut 103 and sealing-ring 104 fixed seal connections with fermentation; The whisking appliance connecting rod 205 of whipping appts 2 covers 101 with fermentation and is rotationally connected through stirring axle sleeve 203, stirring blind nut 204; Paddle wheel 201 is welded on the whisking appliance connecting rod 205 with stirring sail 202; The ring groove of cylindrical filtration unit 3 bottoms and tank body 105 bottoms blocks fixing, and filtering net 301 is filtered the interior cover 303 of net and is fixed on the filter screen stand 302 with filtering net overcoat 304.
This utility model is the center with fermentor tank 1, and the fermentation face of covering has six holes and is respectively whisking appliance connecting rod passage, damping fluid feeding hole and feed hole, gas input hole, fermentation gas collection hole and chyme taphole.Whipping appts 2 and filtration unit 3 are housed in the tank body.Whipping appts 2 is used for regularly stirring feed so that ferment, and filtration unit 3 is used to distinguish solid phase chyme and liquid phase chyme.The liquid phase chyme that produces during fermentation flows out from the chyme taphole, and the solid phase chyme is stayed in the filter screen, and the gas that fermentation simultaneously produces flows out from the fermentation gas collection hole.
During work, at first feed quantitatively is fed into the present invention from drawing-in device 4, feeds damping fluid and CO 2, energized and heating, stirring system regularly stirs, and the feed in the fermentation unit begins fermentation.The liquid phase chyme that produces during fermentation flows out from the chyme taphole, and the solid phase chyme is stayed in the filtration unit; The gas that fermentation simultaneously produces flows out from the fermentation gas collection hole.So just accomplish the rumen zymosis simulation process one time.
The utility model advantage is that single outflow cud in-vitro cultivation fermenting process is easy to control, is convenient to operation; And good airproof performance; The chyme that produces is easy to separate and collect with gas, and the assurance simulation process is accomplished smoothly.
Description of drawings
Fig. 1 is the axonometric exploded view of the utility model;
Fig. 2 is the principle of work front view that the utility model is connected with drawing-in device;
Fig. 3 is that vertical view, a. feed hole are covered in the utility model fermentation; B. fermentation gas collection hole c. gas input hole.D. chyme taphole; E. whisking appliance connecting rod passage; F. damping fluid feeds the hole; G. screw hole is totally 4;
Fig. 4 is the wiring layout of the utility model whipping appts;
Fig. 5 is the paddle wheel vertical view of the utility model whipping appts.
Embodiment
The fermentation of fermentor tank 1 lid 101 is fixedly connected through hollow coupling shaft with drawing-in device 4; Tank body 105 covers 101 through screw 102, nut 103 and sealing-ring 104 fixed seal connections with fermentation; The whisking appliance connecting rod 205 of whipping appts 2 covers 101 with fermentation and is rotationally connected through stirring axle sleeve 203, stirring blind nut 204; Paddle wheel 201 is welded on the whisking appliance connecting rod 205 with stirring sail 202; The ring groove of cylindrical filtration unit 3 bottoms and tank body 105 bottoms blocks fixing, and filtering net 301 is filtered the interior cover 303 of net and is fixed on the filter screen stand 302 with filtering net overcoat 304.

Claims (1)

1. single outflow cud in-vitro cultivation system fermentation unit; It is characterized in that: the fermentation lid (101) of fermentor tank (1) is fixedly connected through hollow coupling shaft with drawing-in device (4); Tank body (105) passes through screw (102), nut (103) and sealing-ring (104) fixed seal connection with fermentation lid (101); The whisking appliance connecting rod (205) of whipping appts (2) is rotationally connected through stirring axle sleeve (203), stirring blind nut (204) and fermentation lid (101); Paddle wheel (201) and stirring sail (202) are welded on the whisking appliance connecting rod (205); The ring groove of cylindrical filtration unit (3) bottom bottom tank body (105) blocks fixing, and filtering net (301) is filtered the interior cover of net (303) and filtering net overcoat (304) is fixed on the filter screen stand (302).
CN 201120231275 2011-07-04 2011-07-04 Single outflow rumen in-vitro continuous culture system fermentation device Expired - Fee Related CN202265565U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201120231275 CN202265565U (en) 2011-07-04 2011-07-04 Single outflow rumen in-vitro continuous culture system fermentation device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201120231275 CN202265565U (en) 2011-07-04 2011-07-04 Single outflow rumen in-vitro continuous culture system fermentation device

Publications (1)

Publication Number Publication Date
CN202265565U true CN202265565U (en) 2012-06-06

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN 201120231275 Expired - Fee Related CN202265565U (en) 2011-07-04 2011-07-04 Single outflow rumen in-vitro continuous culture system fermentation device

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CN (1) CN202265565U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104893957A (en) * 2015-06-15 2015-09-09 湖南农业大学 Continuous fermentation device capable of simulating rumen digestion

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104893957A (en) * 2015-06-15 2015-09-09 湖南农业大学 Continuous fermentation device capable of simulating rumen digestion

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C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20120606

Termination date: 20130704