CN201567334U - Rumen in-vitro continuous culture device - Google Patents
Rumen in-vitro continuous culture device Download PDFInfo
- Publication number
- CN201567334U CN201567334U CN 200920094956 CN200920094956U CN201567334U CN 201567334 U CN201567334 U CN 201567334U CN 200920094956 CN200920094956 CN 200920094956 CN 200920094956 U CN200920094956 U CN 200920094956U CN 201567334 U CN201567334 U CN 201567334U
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- stainless steel
- damping fluid
- fixedly connected
- fermentation culture
- culture device
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
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Abstract
The utility model relates to a rumen in-vitro continuous culture device which belongs to the field of analog bio-simulation machinery, wherein the top of a stainless steel framework is fixedly connected with a buffer solution conveying device, a gas introduction device is fixedly connected with the stainless steel framework, a peristaltic pump 1 is fixedly connected with the internal portion of the stainless steel framework, a stepping motor is fixedly connected with a stirring device, a stirrer at the lower end of the stirring device is positioned in a fermentation culture device which is located in a water-bath box 1, a chyme collecting device is positioned in a water-bath box 2, a peristaltic pump 2 is connected with chyme collecting pipe, a gas collecting device is fixedly connected with the fermentation culture device, and a feeding device is also fixedly connected with the fermentation culture device. The rumen in-vitro continuous culture device has the advantages that the structure is novel, the rumen in-vitro continuous culture process is easy to control and convenient for operation; the leakproofness is excellent; the produced chyme and gas are easy to separate and collect, and the simulation process is guaranteed to be finished successfully.
Description
Technical field
The utility model belongs to simulation bionic mechanical field, refers in particular to the design of environment in the cud, the simulator of realization ruminal digestion.
Background technology
Along with the day by day attention of people to ruminant tumor gastric research, the device outside design of research rumen zymosis more and more trends towards the simulation to the rumen zymosis truth.Therefore, various in vitro artificial rumen methods are arisen at the historic moment.In vitro method is divided into batch culture method and continuous culture method by its fermenting characteristic.Batch culture method, i.e. the disposable adding of microbial inoculant thing and fermentation substrate after certain hour is cultivated, finishes to cultivate in the set time.Owing to degradation reason under product inhibition, the pH, the vigor that batch cultivation has microorganism descends, forms problem appearance such as variation; And the in-vitro cultivation method has overcome this shortcoming, has realized the continuous discharge of tunning in the vitro culture system, can keep damping fluid continuously to flow into fermenting container simultaneously, with the effect of simulation ruminating animal saliva.Because These characteristics, in-vitro cultivation method be the interior environment of simulation rumen more accurately, for quantitative examination cud physiology, biological chemistry and microbiology etc. provide a comparatively ideal model.But present shortage is the device of the interior environment of simulation rumen exactly, and indexs such as specified rate research cud physiology, biological chemistry and microbiology are brought difficulty.
Summary of the invention
The utility model provides a kind of cud in-vitro cultivation device, and to solve the problem of the outer cud simulator of present shortage, purpose is to make the physiology of environment in the cud, the simulation that biological process becomes to become a reality.The technical scheme that the utility model is taked is: the fixedly connected damping fluid e Foerderanlage in the top of stainless steel frame, the gas gatherer is fixedlyed connected with stainless steel frame, the structure of this gas gatherer is that gas introduction tube is connected with gas distributor, this gas distributor is connected with gas service pipes, this gas service pipes is connected with the fermentation culture device with the damping fluid bottle of damping fluid e Foerderanlage respectively, the structure of this damping fluid e Foerderanlage is: damping fluid bottle bottom is connected with stainless steel frame by support, this damping fluid bottle bottom is connected with the damping fluid divider by little sealing plug, this damping fluid divider is connected with the damping fluid transfer lime, peristaltic pump one is connected with this stainless steel frame internal fixation, peristaltic pump connects the damping fluid transfer lime respectively and is fixedly connected on the fermentation culture device of stainless steel frame inside, stepper-motor is fixedly connected in the top stainless steel frame of this fermentation culture device, this stepper-motor is fixedlyed connected with whipping appts, this whipping appts lower end agitator is positioned at the fermentation culture device, stirring axle sleeve and stirrer shaft is rotationally connected, stirring blind nut is connected with the stir shaft external threading, the fermentation culture device is positioned at water bath one, heating unit is connected with the stainless steel frame internal fixation, this heating unit is connected with water bath one, the chyme collection device is positioned at water bath two, this water bath two is fixedlyed connected with the stainless steel frame bottom, refrigeration plant is connected with the stainless steel frame internal fixation, this refrigeration plant is connected with water bath two, peristaltic pump two is connected with the chyme collection tube, this chyme collection tube one end is fixedlyed connected with the fermentation culture device, the other end is fixedlyed connected with the chyme collection device, gas collector is fixedlyed connected with the fermentation culture device, and drawing-in device is fixedlyed connected with the fermentation culture device.
The structure of the utility model drawing-in device is: feed tank is fixedly connected with the hollow coupling shaft that is connected the fermentation culture device, fixedly connected with sealing cover in feed tank top, 4-8 feeding hopper arranged in the feed tank, fixedly connected with the feeding hopper pallet in this feeding hopper middle part, this feeding hopper bottom is fixed on the spout sealing pad, spout sealing pad and feed tank bottom are rotationally connected, and the feeding control lever is rotationally connected with feed tank with feeding hopper pallet, the cross-under of spout sealing pad, its lower end respectively, fixedly connected with handwheel in its top.
The structure of the utility model fermentation culture device is: fermentor tank is threaded with the fermentation cover, the fermentor tank internal fixation connects filters rack, filtering net is connected with this filtration rack, and fermentor tank is stamped connection drawing-in device hole, connection gas collector hole, connection gas input device hole, connection chyme collection device hole, connects whipping appts hole, connection damping fluid input unit hole.
The structure of the utility model gas collector is: the fixedly connected collector lid in collection tube top has the one-way exhaust valve mouth on this collection tube.
This device is the center with the fermentor tank in the fermentation culture device, whipping appts and the filter screen that is used to distinguish solid phase and liquid phase are housed in jar, whipping appts adopts stepper-motor to finish in the mode that 27r/min drives two groups of impellers in each jar, and has an electronic timing agitating function, usually stir 25min, stop 5min; The damping fluid that system uses inputs in the fermentor tank by the peristaltic pump of buffer conveyor device is a certain amount of, and feed link is crossed drawing-in device and fed at regular time and quantity and give; Liquid phase chyme effluent liquid is collected in the interior bottle of receiving flask of temperature automatically controlled 0～4 ℃ collection device, and its flow is by peristaltic pump two controls; This device has 12 fermentor tanks; The heating of fermentor tank and temperature control adopt equipment such as concentrated water bath that heats and attachment means to finish.
During work, drawing-in device of the present utility model carries out regularly, quantitative feeding, and by the stirring of stirring system, after the fermentation system fermentation, the liquid phase chyme of generation is collected in the receiving flask, and the solid phase chyme is stayed in the filtering net.So just finish the ruminal digestion simulation process one time.
The utility model advantage is novel structure, and cud in-vitro cultivation process is easy to control, is convenient to operation; And good airproof performance; The chyme that produces is easy to separate and collect with gas, and the assurance simulation process is finished smoothly.
Description of drawings
Fig. 1 is the utility model structural representation;
Fig. 2 is the utility model left view;
Fig. 3 is the utility model fermentation cover figure, mainly expresses fermentor tank and has covered 6 holes;
Fig. 4 is the structural representation of the utility model feeding, fermentation and three devices of stirring;
Fig. 5 is the utility model gas collector structural representation.
Embodiment
The fixedly connected damping fluid e Foerderanlage 3 in the top of stainless steel frame 8, gas gatherer 4 is fixedlyed connected with stainless steel frame, the structure of this gas gatherer is that gas introduction tube 403 is connected with gas distributor 401, this gas distributor is connected 402 with gas service pipes, this gas service pipes is connected with fermentation culture device 1 with the damping fluid bottle of damping fluid e Foerderanlage 3 respectively, the structure of this damping fluid e Foerderanlage is: damping fluid bottle 301 bottoms are connected with stainless steel frame by support 302, this damping fluid bottle bottom is connected with damping fluid divider 304 by little sealing plug 303, this damping fluid divider is connected with damping fluid transfer lime 305, peristaltic pump 1 is connected with this stainless steel frame internal fixation, peristaltic pump 11 connects damping fluid transfer lime 305 respectively and is fixedly connected on the fermentation culture device 1 of stainless steel frame inside, stepper-motor 12 is fixedly connected in the top stainless steel frame of this fermentation culture device, this stepper-motor is fixedlyed connected with whipping appts 2, this whipping appts lower end agitator 201 is positioned at the fermentation culture device, stirring axle sleeve 202 is rotationally connected with stirrer shaft, stirring blind nut 203 is connected with the stir shaft external threading, fermentation culture device 1 is positioned at water bath 1, heating unit 15 is connected with the stainless steel frame internal fixation, this heating unit is connected with water bath one, chyme collection device 5 is positioned at water bath 29, this water bath two is fixedlyed connected with the stainless steel frame bottom, refrigeration plant 14 is connected with the stainless steel frame internal fixation, this refrigeration plant is connected with water bath two, peristaltic pump 2 13 is connected with chyme collection tube 501, this chyme collection tube one end is fixedlyed connected with fermentation culture device 1, the other end is fixedlyed connected with chyme collection device 5, gas collector 6 is fixedlyed connected with the fermentation culture device, and drawing-in device 7 is fixedlyed connected with the fermentation culture device.
The structure of the utility model drawing-in device is: feed tank 707 is fixedly connected with the hollow coupling shaft 708 that is connected the fermentation culture device, fixedly connected with sealing cover 703 in feed tank top, 4-8 feeding hopper 705 arranged in the feed tank, fixedly connected with feeding hopper pallet 704 in this feeding hopper middle part, this feeding hopper bottom is fixed on the spout sealing pad 706, spout sealing pad and feed tank bottom are rotationally connected, and feeding control lever 702 is rotationally connected with feed tank with feeding hopper pallet, the cross-under of spout sealing pad, its lower end respectively, fixedly connected with handwheel 701 in its top.
The structure of the utility model fermentation culture device is: fermentor tank 104 is threaded with fermentation cover 101, the fermentor tank internal fixation connects filters rack 102, filtering net 103 is connected with this filtration rack, and fermentor tank is stamped connection drawing-in device hole 105, connection gas collector hole 106, connection gas input device hole 107, connection chyme collection device hole 108, connects whipping appts hole 109, connection damping fluid input unit hole 110.
The structure of the utility model gas collector 6 is: the fixedly connected collector lid 601 in collection tube 603 tops has one-way exhaust valve mouth 602 on this collection tube.
Claims (4)
1. cud in-vitro cultivation device, it is characterized in that: the fixedly connected damping fluid e Foerderanlage in the top of stainless steel frame, the gas gatherer is fixedlyed connected with stainless steel frame, the structure of this gas gatherer is that gas introduction tube is connected with gas distributor, this gas distributor is connected with gas service pipes, this gas service pipes is connected with the fermentation culture device with the damping fluid bottle of damping fluid e Foerderanlage respectively, the structure of this damping fluid e Foerderanlage is: damping fluid bottle bottom is connected with stainless steel frame by support, this damping fluid bottle bottom is connected with the damping fluid divider by little sealing plug, this damping fluid divider is connected with the damping fluid transfer lime, peristaltic pump one is connected with this stainless steel frame internal fixation, peristaltic pump connects the damping fluid transfer lime respectively and is fixedly connected on the fermentation culture device of stainless steel frame inside, stepper-motor is fixedly connected in the top stainless steel frame of this fermentation culture device, this stepper-motor is fixedlyed connected with whipping appts, this whipping appts lower end agitator is positioned at the fermentation culture device, stirring axle sleeve and stirrer shaft is rotationally connected, stirring blind nut is connected with the stir shaft external threading, the fermentation culture device is positioned at water bath one, heating unit is connected with the stainless steel frame internal fixation, this heating unit is connected with water bath one, the chyme collection device is positioned at water bath two, this water bath two is fixedlyed connected with the stainless steel frame bottom, refrigeration plant is connected with the stainless steel frame internal fixation, this refrigeration plant is connected with water bath two, peristaltic pump two is connected with the chyme collection tube, this chyme collection tube one end is fixedlyed connected with the fermentation culture device, the other end is fixedlyed connected with the chyme collection device, gas collector is fixedlyed connected with the fermentation culture device, and drawing-in device is fixedlyed connected with the fermentation culture device.
2. cud in-vitro cultivation device according to claim 1, it is characterized in that: the structure of drawing-in device is that feed tank is fixedly connected with the hollow coupling shaft that is connected the fermentation culture device, fixedly connected with sealing cover in feed tank top, 4-8 feeding hopper arranged in the feed tank, fixedly connected with the feeding hopper pallet in this feeding hopper middle part, this feeding hopper bottom is fixed on the spout sealing pad, spout sealing pad and feed tank bottom is rotationally connected, the feeding control lever respectively with the feeding hopper pallet, the cross-under of spout sealing pad, its lower end and feed tank are rotationally connected, fixedly connected with handwheel in its top.
3. cud in-vitro cultivation device according to claim 1, it is characterized in that: the structure of fermentation culture device is: fermentor tank is threaded with the fermentation cover, the fermentor tank internal fixation connects filters rack, filtering net is connected with this filtration rack, and fermentor tank is stamped connection drawing-in device hole, connection gas collector hole, connection gas input device hole, connection chyme collection device hole, connects whipping appts hole, connection damping fluid input unit hole.
4. cud in-vitro cultivation device according to claim 1, it is characterized in that: the structure of gas collector is: the fixedly connected collector lid in collection tube top has the one-way exhaust valve mouth on this collection tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200920094956 CN201567334U (en) | 2009-12-11 | 2009-12-11 | Rumen in-vitro continuous culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200920094956 CN201567334U (en) | 2009-12-11 | 2009-12-11 | Rumen in-vitro continuous culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201567334U true CN201567334U (en) | 2010-09-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200920094956 Expired - Fee Related CN201567334U (en) | 2009-12-11 | 2009-12-11 | Rumen in-vitro continuous culture device |
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| Country | Link |
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| CN (1) | CN201567334U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174367A (en) * | 2011-01-13 | 2011-09-07 | 王之盛 | Artificial rumen device used for continuous multiple determination of pH value |
| CN107705686A (en) * | 2017-11-07 | 2018-02-16 | 凯奇集团有限公司 | Ruminant digestion apparatus for demonstrating |
| CN110499242A (en) * | 2019-09-24 | 2019-11-26 | 扬州大学 | A kind of simulator and its analogy method of cud epithelial tissue in vitro culture |
-
2009
- 2009-12-11 CN CN 200920094956 patent/CN201567334U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174367A (en) * | 2011-01-13 | 2011-09-07 | 王之盛 | Artificial rumen device used for continuous multiple determination of pH value |
| CN107705686A (en) * | 2017-11-07 | 2018-02-16 | 凯奇集团有限公司 | Ruminant digestion apparatus for demonstrating |
| CN110499242A (en) * | 2019-09-24 | 2019-11-26 | 扬州大学 | A kind of simulator and its analogy method of cud epithelial tissue in vitro culture |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100901 Termination date: 20131211 |