CN202237801U - Filtering device for separating radioactively-labeled substances - Google Patents
Filtering device for separating radioactively-labeled substances Download PDFInfo
- Publication number
- CN202237801U CN202237801U CN2011203687722U CN201120368772U CN202237801U CN 202237801 U CN202237801 U CN 202237801U CN 2011203687722 U CN2011203687722 U CN 2011203687722U CN 201120368772 U CN201120368772 U CN 201120368772U CN 202237801 U CN202237801 U CN 202237801U
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- filter
- bottle
- receiving flask
- liquid discharge
- hole
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Abstract
The utility model discloses a filtering device for separating radioactively-labeled substances. The filtering device comprises a filter and is characterized in that: a collection bottle is arranged below the filter; a bottle plug is arranged at the bottle opening of the collection bottle; two liquid discharge tubes are connected at the lower part of the filter; the liquid discharge tubes pass through the bottle plug and extend into the collection bottle; a control valve is arranged on each liquid discharge tube above the bottle plug; a separation board arranged between the two liquid discharge tubes in the collection bottle is used for dividing a space inside the bottle into two cavities; the bottoms of the two cavities are not communicated with each other; a liquid discharge hole is arranged at the bottom of each cavity of the liquid collection bottle; a through hole is arranged at the position of the collection bottle, which is close to the bottle opening; and a vacuumizing safety device is connected with the through hole. By arranging the two liquid discharge tubes below the filter disclosed by the utility model, labeled substances and non-labeled substances are respectively discharged into the cavities in the collection bottle so as to separate the labeled substances from the non-labeled substances. The entire filtering device has the advantages of simple structure, convenience for use and lower cost.
Description
Technical field
The utility model relates to a kind of filter, is specifically related to the filter that a kind of radioactive marker substance of being used for separates.
Background technology
In the prior art; Radioactive substance and labeled compound thereof all play an important role in military, industry, agricultural, medical science and each scientific research field; In many research work of life science, the radio-labelled compound research tool that is absolutely necessary.For example, the mechanism of action of enzyme and analysis, cytology, molecular biology, receptors bind research, radiommunoassay, pharmacokinetics, and the diagnosis of cancer and treatment etc. all be unable to do without radio-labelled compound.
In experiment; Sometimes need radioactive substance on the mark and material on unmarked be separated; Conventional device can only be collected the sample on these microtest plates like 12 holes, 24 holes, 96 porocyte gatherers etc. in the laboratory; And can not disposable a large amount of collection culture dishes, more sample in the blake bottle, test tube, more can not be used for the collection of radioactivity deposition; In addition, the easy plug-hole of these cell harvestors, the difficult cleaning brought bigger experimental error; Fraction collector is that the radioactive liquid waste after filtering is not added differentiation, all is collected in together, causes increasing of radwaste, has increased the difficulty and the expense of processing of waste.
From the above, design a kind of easy, reasonable, multi-functional filter, become to be solved having asked of urgency in the industry so that disposable radioactive substance with a large amount of marks is separated.
Summary of the invention
The goal of the invention of the utility model provides a kind of filter of simple in structure, easy to use and lower-cost radioactive marker substance of being used for and unmarked separating substances.
For reaching the foregoing invention purpose; The technical scheme that the utility model adopts is: the filter that a kind of radioactive marker substance of being used for separates, comprise a filter, and said filter below is provided with receiving flask; Said receiving flask bottle mouth position is provided with bottle stopper; Said filter bottom is connected with two discharging tubes, and said discharging tube runs through said bottle stopper and extends in the said receiving flask, is positioned at place, bottle stopper top on the said discharging tube and is provided with control valve; Be positioned in the said receiving flask and be provided with dividing plate between two discharging tubes bottle is separated into the cavity volume that two bottoms do not communicate; Said receiving flask bottom is positioned at two cavity volume places and offers outage respectively, and the nearly bottleneck of said receiving flask offers through hole, is connected with said through hole and vacuumizes safety device.
Further technical scheme; The said safety device that vacuumizes comprises vavuum pump and safety flack, and said safety flack bottle mouth position is provided with bottle stopper, is penetrated with two tube connectors on the said bottle stopper; One of them tube connector in the said tube connector is connected with said vavuum pump, and another tube connector is connected with said through hole.
Further technical scheme, said filter outside is provided with fixed support.
In the technique scheme; This device is made up of filter, receiving flask, fixed support, safety flack, vavuum pump, and the characteristics of this filter are that the bottom has two discharging tubes to run through bottle stopper to stretch in the receiving flask, is positioned at the bottle stopper top on the discharging tube and is provided with control valve; The receiving flask middle part is divided into two cavity volumes that the bottom does not communicate; Receive radioactive marker substance and unmarked material respectively, the receiving flask bottom is positioned at two cavity volume places respectively has two outages, is beneficial to waste liquid and discharges; Receiving flask, safety flack and vavuum pump are linked to each other by tube connector, and vavuum pump is used for safety flack and receiving flask inside are evacuated.This device in use; Utilize vavuum pump that safety flack and receiving flask are vacuumized; Open the control valve on one of them discharging tube; When the filter membrane of macromolecular substances such as the nucleic acid of radioactivity deposition or mark radioactive substance, protein through certain pore size on the filter, above-mentioned macromolecular substances is retained on the filter membrane, and unconjugated free little molecule radioactive substance flow in one of them cavity volume in the receiving flask through the discharging tube of opening; The waste liquid of having collected can be discharged through the outage of bottom easily; Macromolecular substances such as the radioactivity deposition that remains or the nucleic acid of mark radioactive substance, protein continue to serve as the usefulness of later experiment, and receiving flask is connected with safety flack through tube connector, can effectively prevent the waste liquid suck-back in the receiving flask; This device is mainly used in the material (like tumour cell, lymphocyte, DNA, RNA, protein, acceptor etc.) of a large amount of collection radioisotope labelings, and the deposition that contains radionuclide.
Because the technique scheme utilization, the utility model compared with prior art has advantage:
1. the utility model is through stretching in the receiving flask respectively through two discharging tubes below filter; Be separated into the cavity volume that two bottoms do not communicate through dividing plate between inherent two discharging tubes of bottle; Two cavity volume bottoms are respectively equipped with outage; Also be connected with safety flack and vavuum pump with receiving flask, filter through filter, through two discharging tubes respectively with mark and unmarked separating substances and enter in the cavity volume of receiving flask; The realization mark separates with unmarked material, and structure is simpler as compared with the past, use is also more convenient;
2. the employed equipment of the utility model device is common on the market equipment, and is cheap, thereby cost is lower, is fit to promote the use of.
Description of drawings
Fig. 1 is the structural representation of the utility model embodiment one.
Wherein: 1, filter; 2, receiving flask; 3, discharging tube; 4, control valve; 5, dividing plate; 6, outage; 7, vavuum pump; 8, safety flack; 9, tube connector; 10, fixed support.
The specific embodiment
Below in conjunction with accompanying drawing and embodiment the utility model is further described:
Embodiment one: referring to shown in Figure 1, a kind of filter that is used for radioactive marker substance's separation comprises a filter 1; Said filter 1 outside is provided with fixed support 10, and said filter 1 below is provided with receiving flask 2, and said receiving flask 2 bottle mouth positions are provided with bottle stopper; Said filter 1 bottom is connected with two discharging tubes 3; Said discharging tube 3 runs through said bottle stopper and extends in the said receiving flask 2, is positioned at bottle stopper top place on the said discharging tube 3 and is provided with control valve 4, is positioned in the said receiving flask 2 to be provided with dividing plate 5 between two discharge opeings, 3 pipes bottle is separated into the cavity volume that two bottoms do not communicate; Said receiving flask 2 bottoms are positioned at two cavity volume places and offer outage 6 respectively; Said receiving flask 2 nearly bottlenecks offer through hole, are connected with said through hole and vacuumize safety device, and the said safety device that vacuumizes comprises vavuum pump 7 and safety flack 8; Said safety flack 8 bottle mouth positions are provided with bottle stopper; Be penetrated with two tube connectors 9 on the said bottle stopper, one of them tube connector in the said tube connector is connected with said vavuum pump, and another tube connector is connected with said through hole.
This device in use; Side cavity volume in the receiving flask can be used for collecting the high radioactivity waste liquid, and the opposite side cavity volume is used to collect the low-activity waste liquid, when macromolecular substances such as the DNA that collects radioisotope labeling, RNA, protein; Vavuum pump vacuumizes safety flack and receiving flask; Open the control valve on the discharging tube of corresponding cavity volume top of collecting the high radioactivity waste liquid simultaneously, reactant liquor contains radioactive macromolecular substances and is retained on the filter membrane through the filter membrane of certain pore size on the filter; Free unmarked radionuclide sees through the filter membrane aperture owing to molecular weight is little; Be collected into the cavity volume that is used to collect the high radioactivity waste liquid in the receiving flask, initial a small amount of cleaning solution also enters the cavity volume of collecting the high radioactivity waste liquid, opens the control valve on the discharging tube of corresponding cavity volume top of collecting the low-activity waste liquid at last; Close the control valve of the discharging tube of corresponding high radioactivity waste liquid cavity volume simultaneously, large batch of cleaning solution is collected the cavity volume that is used for the low-activity waste liquid in the receiving flask.When separating radioactive substance with coprecipitation; Open the control valve of discharging tube of the cavity volume top of corresponding low-activity waste liquid; Reactant liquor is through the filter membrane on the filter; Contain radioactive deposition and be retained on the filter membrane, remaining reaction liquid and cleaning solution are collected into the cavity volume that is used for the low-activity waste liquid in the receiving flask.
Claims (3)
1. one kind is used for the filter that the radioactive marker substance separates; Comprise a filter (1); It is characterized in that: said filter (1) below is provided with receiving flask (2), and said receiving flask (2) bottle mouth position is provided with bottle stopper, and said filter (1) bottom is connected with two discharging tubes (3); Said discharging tube (3) runs through said bottle stopper and extends in the said receiving flask (2); Be positioned at bottle stopper top place on the said discharging tube (3) and be provided with control valve (4), be positioned in the said receiving flask (2) and be provided with dividing plate (5) between two discharging tubes (3) bottle is separated into the cavity volume that two bottoms do not communicate, said receiving flask (2) bottom is positioned at two cavity volume places and offers outage (6) respectively; The nearly bottleneck of said receiving flask (2) offers through hole, is connected with said through hole and vacuumizes safety device.
2. a kind of filter that the radioactive marker substance separates that is used for according to claim 1; It is characterized in that: the said safety device that vacuumizes comprises vavuum pump (7) and safety flack (8); Said safety flack (8) bottle mouth position is provided with bottle stopper; Be penetrated with two tube connectors (9) on the said bottle stopper, one of them tube connector in the said tube connector (9) is connected with said vavuum pump (7), and another tube connector is connected with said through hole.
3. a kind of filter that the radioactive marker substance separates that is used for according to claim 1, it is characterized in that: said filter (1) outside is provided with fixed support (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203687722U CN202237801U (en) | 2011-09-30 | 2011-09-30 | Filtering device for separating radioactively-labeled substances |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203687722U CN202237801U (en) | 2011-09-30 | 2011-09-30 | Filtering device for separating radioactively-labeled substances |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202237801U true CN202237801U (en) | 2012-05-30 |
Family
ID=46099106
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011203687722U Expired - Fee Related CN202237801U (en) | 2011-09-30 | 2011-09-30 | Filtering device for separating radioactively-labeled substances |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202237801U (en) |
-
2011
- 2011-09-30 CN CN2011203687722U patent/CN202237801U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120530 Termination date: 20140930 |
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| EXPY | Termination of patent right or utility model |