CN202159065U - Reagent kit used for detecting albumin in urine of mammals - Google Patents
Reagent kit used for detecting albumin in urine of mammals Download PDFInfo
- Publication number
- CN202159065U CN202159065U CN2010206266129U CN201020626612U CN202159065U CN 202159065 U CN202159065 U CN 202159065U CN 2010206266129 U CN2010206266129 U CN 2010206266129U CN 201020626612 U CN201020626612 U CN 201020626612U CN 202159065 U CN202159065 U CN 202159065U
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- kit
- urine
- albumin
- nitrocellulose filter
- lower house
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Abstract
The utility model discloses a reagent kit used for detecting albumin in urine of mammals, which comprises a urine albumin testing biological chip and a shell arranged outside the urine albumin testing biological chip which comprises a bottom plate, a cellulose nitrate film arranged in the middle of the bottom plate, a sample pad and water sucking filter paper, wherein the sample pad and the water sucking filter paper are respectively arranged at two ends of the bottom plate. The reagent kit used for detecting albumin in the urine of the mammals can be used for making auxiliary judgment of renal injury diseases of the mammals. Compared with the prior art, the reagent kit used for detecting albumin in the urine of the mammals has the advantages of being simple and convenient to use, easy to store, visual in result and the like.
Description
Technical field
The utility model relates to molecular biology, immunology and protein detection technology field.More specifically, relate to a kind of can the qualitative detection mammal urine in albuminous kit.
Background technology
(microalbunminuria MAU) is meant that (Albumin, ALB) content exceeds the healthy subjects term of reference to albumin in the urine, but can not detect the variation of this trace with the method for routine to microalbuminuria.In order to make this detection index standardization, adopt the albumin secretion rate to represent albuminous discharge rate in the urine in the world.Healthy subjects MAU is in the scope of＜20mg-30mg/24h; MAU is called albuminuria when 30-300mg/24h; Be called a large amount of albuminurias during MAU＞300mg/24h.It is the early stage change of disease that albuminuria is discharged increase, and to the early diagnosis of disease, early treatment has important value and clinical meaning.
The urine microalbumin has the panimmunity chemistry in detecting, like semiquantitative test paper bar method, ELISA albumin, radiommunoassay, immunoturbidimetry mensuration, time-resolved fluorescent immunoassay etc.The key of chemistry in detecting is the specific bond between antigen-antibody, and how with the signal of this specific bond through the amplification that gains of certain technological means.Semiquantitative test paper bar method need be by the instrument sentence read result; Though ELISA is also sensitive, special, human factor usually influences the result of mensuration; Though radiation is exempted from method and measured sensitivity, special, there is the alpha-contamination problem of how handling; Though it is pollution-free that immunoturbidimetry is measured, its requirement to instrument is high especially; Time-resolved fluorescent immunoassay needs supporting instrument and reagent more.The Array 360 that present domestic over-borrowing helps Beckman company comes the microalbumin in the urine is analyzed.But said method all can not satisfy the requirement of accurately measuring fast of present clinical great amount of samples or small number of samples on the whole.
The utility model content
The utility model provides a kind of can detect albuminous kit in the mammal urine quickly and accurately, has advantages such as easy to use, easy storage, visual result.
The utility model kit comprises mammal urinary albumin test organisms chip, and is arranged at its outer shell;
Above-mentioned urinary albumin test organisms chip comprises base plate, is arranged on the nitrocellulose filter at this base plate middle part, and the sample pad and the absorbent filter that are separately positioned on these base plate two ends.
On the above-mentioned nitrocellulose filter, from sample pad one end, the compartment of terrain is provided with collaurum pad, p-wire and control line successively; Said collaurum pad is made up of the collaurum-ALB antibody composite bed that encapsulates on nitrocellulose filter; Said p-wire is made up of the mammal albumin composite bed that is coated on the nitrocellulose filter, and said control line is made up of the sheep anti-mouse antibody composite bed that is coated on the nitrocellulose filter.
Above-mentioned shell comprise upper shell and with the lower house of the corresponding clamping of upper shell, said urinary albumin test organisms chip is arranged in the lower house.Upper shell and lower house can connect and fix through reference column and pilot hole correspondence.
Upper shell and sample pad correspondence position are provided with well; With the corresponding position of p-wire and control line, be provided with watch window; Between well and watch window, be provided with and press down the stream valve; Relevant position at the lower house corresponding with pressing down the stream valve is provided with reservoir.
Above-mentioned urinary albumin test organisms chip is arranged in the lower house, in the relevant position of the lower house corresponding with sample pad, is provided with at least one reservoir, and the upper shell position corresponding with reservoir is provided with press down corresponding with reservoir and flows valve.
Be provided with drying agent in the above-mentioned lower house.
The utility model is through pressing down the setting of stream valve and reservoir, make tracer liquid can be in test-strips even chromatography, make testing result more reliable.The utility model can be made auxiliary judgment to the mammal kidney damage disease, and is compared with prior art more intuitively easy-to-use.
Below in conjunction with accompanying drawing and embodiment the utility model is explained further details, but accompanying drawing and embodiment do not constitute any restriction to the utility model.Those skilled in the art all should be included within the utility model scope reading general change or the replacement of being done after the utility model content in this area.
Description of drawings
Fig. 1 is the structural representation of the shell of the utility model;
Fig. 2 is the inner structure of the utility model and connects synoptic diagram.
Each label implication of Fig. 1 and Fig. 2 is following:
1. upper shell 2. watch windows 3. press down stream valve 4. reference columns 5. wells 6. pilot holes 7. sample pad 8. base plates 9. reservoirs 10. collaurum pads 11. lower houses 12. nitrocellulose filters 13. p-wires 14. control lines 15. drying agent 16. absorbent filters 17. urinary albumin test organisms chips 18. shells
Embodiment
Like Fig. 1 and Fig. 2, the utility model kit comprises urinary albumin test organisms chip 17, and is arranged at its outer shell 18; Said urinary albumin test organisms chip 17 comprises base plate 8, and is arranged on the nitrocellulose filter 12 at these base plate 8 middle parts and is separately positioned on the sample pad 7 and absorbent filter 16 at these base plate 8 two ends.Base plate 8 adopts pvc material.
On the above-mentioned nitrocellulose filter 12, from sample pad 7 one ends, the compartment of terrain is provided with collaurum pad 10, p-wire 13 and control line 14 successively; Said collaurum pad 10 is encapsulated and is formed by the collaurum-ALB antibody composite bed that is adsorbed on the nitrocellulose filter 12; Said p-wire 14 is made up of the mammal albumin composite bed that is coated on the nitrocellulose filter 12, and said control line 14 is made up of the sheep anti-mouse antibody composite bed that is coated on the nitrocellulose filter 12.
Above-mentioned shell 18 comprises upper shell 1 and lower house 11, and the two connects and fixes through reference column 4 that is arranged on upper shell 1 and pilot hole 6 correspondences that are arranged on the lower house 11.Upper shell 1 and sample pad 10 correspondence positions are provided with well 5; With p-wire 13 and control line 14 correspondence positions, be provided with watch window 2; Between well 5 and watch window 2, be provided with and press down stream valve 3.
Above-mentioned urinary albumin test organisms chip 17 is arranged in the lower house 11, and the relevant position at the lower house 11 corresponding with sample pad 7 is provided with reservoir 9, and upper shell 1 position corresponding with reservoir 9 is provided with corresponding with the reservoir 9 stream valve 3 that presses down.
In the above-mentioned lower house 11, with urinary albumin test organisms chip 17 position independently mutually, be provided with drying agent 15.
Claims (7)
1. one kind is used for the kit that the mammal urine albumin detects, and it is characterized in that, comprises mammal urinary albumin test organisms chip, and is arranged at its outer shell.
2. kit as claimed in claim 1 is characterized in that, said mammal urinary albumin test organisms chip comprises base plate, and the nitrocellulose filter that is arranged on this base plate middle part, and the sample pad and the absorbent filter that are separately positioned on these base plate two ends.
3. kit as claimed in claim 1 is characterized in that, on the said nitrocellulose filter, from sample pad one end, the compartment of terrain is provided with collaurum pad, p-wire and control line successively.
4. kit as claimed in claim 1; It is characterized in that; Said collaurum pad is made up of the collaurum-ALB antibody composite bed that encapsulates on nitrocellulose filter; Said p-wire is made up of the mammal albumin composite bed that is coated on the nitrocellulose filter, and said control line is made up of the sheep anti-mouse antibody composite bed that is coated on the nitrocellulose filter.
5. kit as claimed in claim 1 is characterized in that, said shell comprise upper shell and with the lower house of the corresponding clamping of upper shell, said urinary albumin test organisms chip is arranged in the lower house.
6. kit as claimed in claim 5 is characterized in that, said upper shell and sample pad correspondence position are provided with well; With the corresponding position of p-wire and control line, be provided with watch window; Between well and watch window, be provided with and press down the stream valve; Relevant position at the lower house corresponding with pressing down the stream valve is provided with reservoir.
7. kit as claimed in claim 5 is characterized in that, is provided with drying agent in the said lower house.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010206266129U CN202159065U (en) | 2010-11-24 | 2010-11-24 | Reagent kit used for detecting albumin in urine of mammals |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010206266129U CN202159065U (en) | 2010-11-24 | 2010-11-24 | Reagent kit used for detecting albumin in urine of mammals |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202159065U true CN202159065U (en) | 2012-03-07 |
Family
ID=45766697
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010206266129U Expired - Fee Related CN202159065U (en) | 2010-11-24 | 2010-11-24 | Reagent kit used for detecting albumin in urine of mammals |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202159065U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106248920A (en) * | 2016-07-01 | 2016-12-21 | 九牧厨卫股份有限公司 | A kind of urine detector and urine examination method thereof |
-
2010
- 2010-11-24 CN CN2010206266129U patent/CN202159065U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106248920A (en) * | 2016-07-01 | 2016-12-21 | 九牧厨卫股份有限公司 | A kind of urine detector and urine examination method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: ALERE (SHANGHAI) DIAGNOSTICS CO., LTD. Free format text: FORMER NAME: INVERNESS MEDICAL SHANGHAI CO., LTD. |
|
| CP03 | Change of name, title or address |
Address after: Shanghai city 201203 libing road Zhangjiang High Tech Park of Pudong New Area No. 151 Building No. 4 floor Patentee after: Meiai Lier (Shanghai) Diagnostics Product Co.,Ltd. Address before: Shanghai city 201203 libing road Zhangjiang High Tech Park No. 151 Building No. 7 Patentee before: Shanghai Inverness Medical Co., Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120307 Termination date: 20191124 |