CN202159021U - Lighting standard board for calibration/detection of chemiluminescent analyzer - Google Patents
Lighting standard board for calibration/detection of chemiluminescent analyzer Download PDFInfo
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- CN202159021U CN202159021U CN 201120197659 CN201120197659U CN202159021U CN 202159021 U CN202159021 U CN 202159021U CN 201120197659 CN201120197659 CN 201120197659 CN 201120197659 U CN201120197659 U CN 201120197659U CN 202159021 U CN202159021 U CN 202159021U
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- emission standards
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- lightening hole
- led lamp
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Abstract
The utility model discloses a lighting standard board for calibration/detection of a chemiluminescent analyzer, which comprises a board body, wherein a plurality of test holes and a plurality of lighting holes arranged in rows are arranged on the board body, the plurality of test holes are distributed on the periphery of one of the lighting holes in circumferential mode and used for completely detecting interference among holes of the chemiluminescent analyzer from all angles, and light-emitting diode (LED) light sources with different wavelength are respectively arranged in the lighting holes. The lighting standard board can adjust lighting intensity and perform multipoint calibration on lighting with different wavelength during detection, thereby being good in use effect for calibration/detection of the chemiluminescent analyzer.
Description
Technical field
The utility model belongs to analytical instrument measurement and calibration and calibrating field, and the emission standards plate is used in particularly a kind of chemiluminescent analyzer calibration/detection.
Background technology
Chemiluminescence is a kind of optical radiation phenomenon that some material is followed in carrying out chemical reaction process.With atriphos (ATP) is example, the intracellular luminescent substance luciferin of fire fly luminescence, and under the catalysis of luciferase, can be by the ATP oxidation in the microbial cell, and discharge photon.Photon numbers (luminous intensity just) can caught and calculate to the detecting device of chemiluminescent analyzer; Because the concentration of luminous intensity and ATP is linear within the specific limits; Therefore can calculate the ATP content in the testing sample, and be converted to the microorganism total amount.
Chemiluminescence analysis is exactly a kind of through detecting the instrument that chemiluminescence intensity carries out the predetermined substance check and analysis; Be a kind of development in recent years chemical analysis method rapidly, come quantitative test reactant (atriphos), catalyzer (some metallic ion) etc. through the Sensitive Detection chemiluminescence intensity.Adopt the instrument of the analytical approach of this highly sensitive, high automation important application to be arranged, for example clinical diagnosis, food security and environmental monitoring etc. in a lot of fields.Yet, in order such analytical instrument to be carried out quality evaluation, testing result calibration, need utilize a kind of standard of measurement, come chemiluminescent analyzer is calibrated/detected.
Existing generally is to adopt the ATP kit to come calibration instrument, but the ATP kit that different company produces prescription is different, luminous intensity and disunity, and also the quality of this kit is subject to the influence of preservation condition, so result of use is unsatisfactory.Employing is also arranged with the luminous calibration plate of LED lamp,, the performance of chemiluminescent analyzer is calibrated with realization through simulating chemical luminescence process as stabilized light source.
The detecting device of chemiluminescent analyzer is photomultiplier (PMT); Because PMT is very sensitive to the wavelength of photon; The illumination of different wave length is mapped on the PMT; The response that obtains is different (blue light＞green glows＞gold-tinted＞ruddiness), and this has just determined that the use wavelength coverage of chemiluminescent analyzer is different, that is to say that the wavelength coverage when chemiluminescence signal exceeds the detectable wavelength coverage of instrument; Even light intensity can not detect light signal very greatly or can only detect very little signal, so detectable wavelength coverage is an important indicator of chemical analyzer.In addition, because the difference of material and manufacture craft, two different instruments, when the same wavelength of detection luminous, the response that obtains also can be different.Because between two PMT difference is arranged, such as, at the 470nm place, the signal value that instrument A records is 4 times of instrument B, maybe be at the 590nm place, and the signal value that instrument A records has only 3 times of instrument B.Because the use wavelength coverage of detecting device is different, this just need carry out the multiple spot calibration in certain wavelength coverage.Through using the multiple spot calibration, draw out a calibration curve.Yet the insufficiency of existing luminous calibration plate makes the luminous calibration plate of this physics can't satisfy the luminous verification requirement of carrying out the multiple spot calibration to different wave length.
The detection range of light intensities difference of different model chemiluminescent analyzer is bigger, is generally 0～10
6RLU in order to guarantee the versatility of emission standards plate, realizes the full light intensity calibration of multiple model chemiluminescent analyzer and detects, and requires the luminous intensity of emission standards plate can be 0～10
9Flexible setting in the RLU scope.But the defective of existing this luminous calibration plate is: luminous intensity can not be regulated, and this just limits on light emitting region to some extent, can not adapt to the calibration of the chemiluminescent analyzer of various different models.
The utility model content
The utility model technical matters to be solved is can not regulate for the luminous intensity that overcomes the luminous calibration plate of prior art; Can not adapt to the calibration of the chemiluminescent analyzer of different model; And can't satisfy the defective that the verification of multiple spot calibration requires is carried out in different wave length luminous, provide a kind of and can regulate and the emission standards plate is used in the luminous chemiluminescent analyzer calibration/detection that also can carry out the multiple spot calibration of different wave length luminous intensity.
The utility model solves above-mentioned technical matters through following technical proposals: a kind of chemiluminescent analyzer is calibrated/is detected and uses the emission standards plate; Its characteristics are; It comprises: a plate body; A plurality of lightening holes that said plate body is provided with a plurality of instrument connections and establishes side by side; Said a plurality of instrument connection circle distribution therein a said lightening hole around, be used between the hole of the said chemiluminescent analyzer of all angles complete detection, disturbing, the led light source with different wave length is set respectively in each said lightening hole.
Preferably; Said a plurality of lightening hole comprises spaced first lightening hole and second lightening hole at least; The quantity of said a plurality of instrument connections is 8, said 8 instrument connections be evenly distributed on said second lightening hole around, be provided with a blue LED lamp in said second lightening hole.As a kind of conversion, also can be spaced 5 lightening holes.
Preferably; Also be provided with the circuit SS on the said plate body; Said circuit SS is used for the switching between first circuit and the second circuit, and wherein, said first circuit is all luminous circuit of all said lightening holes of control; Said second circuit is that a control has only when said a plurality of instrument connections surround a lightening hole, the circuit that this lightening hole is just luminous.The light intensity of led light source is controlled by circuit voltage, can apply voltage through adjusting, changes the light intensity of lightening hole on the emission standards plate.
Preferably, also be provided with one on the said plate body and be used to regulate the voltage increase of all said led light sources or the voltage-regulation button that reduces, and the rechargeable battery of a pair of said emission standards plate energy supply.
Preferably, the voltage-regulation scope of said led light source is 0～2V.
Preferably, said plate body side is provided with a socket for said rechargeable battery charging, and one is used to indicate battery charger to make the charge prompting lamp of accomplishing.
Preferably, also be provided with a power switch and a capacity prompt lamp on the said plate body.
Preferably, also be provided with a display and a monitor switch on the said plate body.
Preferably, the quantity of said a plurality of lightening holes is 5, is respectively equipped with a purple LED lamp, a blue LED lamp, a green light LED lamp, a yellow light LED lamp and a red-light LED lamp in each said lightening hole.
Preferably, the lighting angle of said led light source is 110 degree.
In the utility model, but above-mentioned optimum condition combination in any on the basis that meets this area general knowledge promptly gets each preferred embodiment of the utility model.
The positive progressive effect of the utility model is: the wavelength that led light source is launched in the lightening hole of the utility model is different, can be used for the detection wavelength of chemiluminescent analyzer is estimated.In addition, the light intensity of led light source is controlled by the voltage that first circuit and the second circuit applied in the lightening hole, thereby can realize that (range of adjustment of light intensity is 0～10 for the adjusting of led light source light intensity
9RLU).In addition, the emission standards plate of the utility model also is provided with eight and detects the hole, can between all angles complete detection hole, disturb, and can satisfy the luminous school inspection requirement of carrying out the multiple spot calibration to different wave length.
Description of drawings
Fig. 1 is the structural representation of the utility model one preferred embodiment.
Fig. 2 is the side view of Fig. 1.
Embodiment
Provide the utility model preferred embodiment below in conjunction with accompanying drawing, to specify the technical scheme of the utility model.
The detection wavelength coverage of chemiluminescent analyzer is generally 300nm～650nm; In order chemiluminescent analyzer to be carried out the multiple spot calibration; The led light source of a plurality of different wave lengths need be installed, and the led light source wavelength distributes evenly in 300nm～650nm scope on the emission standards plate.As depicted in figs. 1 and 2, the emission standards plate of the utility model can be centered around around certain lightening hole 2 being provided with row five lightening holes 2 and 3, eight instrument connections 3 of eight instrument connections on the plate body 1, is used to detect between the hole of chemiluminescent analyzer disturb.A led light source all is set in each lightening hole 2, and the wavelength that each led light source is launched is different, and wavelength evenly distributes in 300nm～650nm scope respectively, to realize the investigation to chemiluminescent analyzer wavelength coverage parameter.
It below is the led light source of the employed different wave length of luminous calibration plate of the utility model; As shown in Figure 1; One purple LED lamp, a blue LED lamp, a green light LED lamp, a yellow light LED lamp and a red-light LED lamp from left to right are set respectively in five lightening holes that are arranged side by side successively; The led light source of different colours corresponding respectively the wavelength of different range: such as; The wavelength coverage that is positioned at the purple LED lamp of first row is 360～365～370, and the wavelength coverage of the blue LED lamp of secondary series is 465～470～475, and the wavelength coverage of tertial green light LED lamp is 520～525～535; The wavelength coverage of the yellow light LED lamp of the 4th row is that the wavelength coverage of the red-light LED lamp of the 587～590～593, the 5th row is 615～620～625.
In order to guarantee the versatility of emission standards plate, realize full light intensity calibration and detection to multiple model chemiluminescent analyzer, therefore, the luminous intensity of the utility model emission standards plate can be 0～10
9Flexible setting in the RLU scope.The led light source that the utility model is selected for use is big lighting angle lamp; And the lighting angle of this big lighting angle lamp is that (lighting angle is meant the angle of 50% luminous intensity to 110 degree; Promptly the vertical direction with lamp is a baseline, about each inclined to one side 55 when spending, the intensity that obtains be vertical direction intensity 50%).In addition, be equipped with even luminescent material, can make whole lightening hole luminous evenly, eliminate the error that causes because of detector location difference between instrument on each led light source top.
Control for the light intensity that makes led light source receives circuit voltage,, on plate body 1, also be provided with circuit SS 4, voltage-regulation button 5, display 6, monitor switch 7, power switch 8 and capacity prompt lamp 9 to realize the adjusting of light source light intensity.
Circuit SS 4 can switch between first circuit and second circuit, and it is all luminous that first circuit is used to control all lightening holes 2; Second circuit control has only that lightening hole (second lightening hole such as as shown in Figure 1 is provided with a blue LED lamp in this lightening hole) that is surrounded by said eight instrument connections 3 just luminous.When all lightening holes 2 are all luminous, carry out wavelength coverage and detect, but when the lightening hole that has only tested prospect hole to surround is luminous, carry out the detection disturbed between strong scope of instrument linearity photometry and hole.Wherein, first circuit and second circuit can adopt and well known to a person skilled in the art in the prior art that circuit realizes that this circuit part is a prior art, does not give unnecessary details at this.
Voltage-regulation button 5 is one group two, is used to regulate the voltage increase of all led light sources or reduce (voltage range 0～2V).
The side of plate body 1 is provided with and is can the energy supply rechargeable battery 10 of emission standards plate to be charged the socket 11 of battery charge and charge prompting lamp 12, and the completion of indication charging work.
Before the emission standards plate that uses the utility model carries out pertinent instruments calibration/calibrating, charge to the emission standards plate, become green until charging indicator light by redness, represent charging process to accomplish, can normally use.The regulating circuit SS at first uses first circuit, makes all lightening holes all luminous.The emission standards plate is put into chemiluminescent analyzer to be detected; And after each detection is accomplished; Regulation voltage is regulated button, through regulating the light intensity of light source works voltage change lightening hole, the full light intensity sensing range of chemiluminescent analyzer is calibrated; And utilize the light source of five kinds of different wave lengths, the detection wavelength of chemiluminescent analyzer is examined and determine.
Regulating circuit SS afterwards; Use second circuit; Have only that lightening hole of the secondary series that is surrounded by eight instrument connections luminous; The luminous intensity through detecting this secondary series lightening hole and the luminous intensity of eight instrument connections on every side, the mean value that eight instrument connections are recorded promptly get divided by the luminous value of the lightening hole of this secondary series between the hole of chemiluminescent analyzer intersects, in view of the above can the hole of analytical chemistry luminescence analyzer between cross pollution.
Below be to adopt the utility model, under basic, normal, high three kinds of different operating voltages, make luminous calibration plate send different luminous intensities, institute carries out the school inspection table of chemiluminescent analyzer repeatability detection:
The testing result of above chemiluminescent analyzer shows: standard deviation is all in 2%.
The advantage of the utility model is that the light intensity of led light source is controlled by the voltage that is applied in the lightening hole, can realize that (range of light intensities is 0～10 for the adjusting of led light source light intensity
9RLU).
Though more than described the embodiment of the utility model, it will be understood by those of skill in the art that these only illustrate, the protection domain of the utility model is limited appended claims.Those skilled in the art can make numerous variations or modification to these embodiments under the prerequisite of principle that does not deviate from the utility model and essence, but these changes and modification all fall into the protection domain of the utility model.
Claims (10)
1. a chemiluminescent analyzer is calibrated/is detected and uses the emission standards plate; It is characterized in that; It comprises: a plate body, a plurality of lightening holes that said plate body is provided with a plurality of instrument connections and establishes side by side, said a plurality of instrument connection circle distribution therein a said lightening hole around; Be used between the hole of the said chemiluminescent analyzer of all angles complete detection, disturbing, the led light source with different wave length is set respectively in each said lightening hole.
2. emission standards plate as claimed in claim 1; It is characterized in that; Said a plurality of lightening hole comprises spaced first lightening hole and second lightening hole at least; The quantity of said a plurality of instrument connections is 8, said 8 instrument connections be evenly distributed on said second lightening hole around, be provided with a blue LED lamp in said second lightening hole.
3. emission standards plate as claimed in claim 1; It is characterized in that also be provided with a circuit SS on the said plate body, said circuit SS is used for the switching between first circuit and the second circuit; Wherein, Said first circuit is all luminous circuit of all said lightening holes of control, and said second circuit is that a control has only when said a plurality of instrument connections surround a lightening hole, the circuit that this lightening hole is just luminous.
4. emission standards plate as claimed in claim 4 is characterized in that, also be provided with one on the said plate body and be used to regulate the voltage increase of all said led light sources or the voltage-regulation button that reduces, and one gives the rechargeable battery of said emission standards plate energy supply.
5. emission standards plate as claimed in claim 4 is characterized in that, the voltage-regulation scope of said led light source is 0～2V.
6. emission standards plate as claimed in claim 4 is characterized in that, said plate body side is provided with a socket for said rechargeable battery charging, and one is used to indicate battery charger to make the charge prompting lamp of accomplishing.
7. emission standards plate as claimed in claim 4 is characterized in that, also is provided with a power switch and a capacity prompt lamp on the said plate body.
8. emission standards plate as claimed in claim 1 is characterized in that, also is provided with a display and a monitor switch on the said plate body.
9. emission standards plate as claimed in claim 1 is characterized in that, the quantity of said a plurality of lightening holes is 5, is respectively equipped with a purple LED lamp, a blue LED lamp, a green light LED lamp, a yellow light LED lamp and a red-light LED lamp in each said lightening hole.
10. emission standards plate as claimed in claim 1 is characterized in that, the lighting angle of said led light source is 110 degree.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120197659 CN202159021U (en) | 2011-06-13 | 2011-06-13 | Lighting standard board for calibration/detection of chemiluminescent analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120197659 CN202159021U (en) | 2011-06-13 | 2011-06-13 | Lighting standard board for calibration/detection of chemiluminescent analyzer |
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| Publication Number | Publication Date |
|---|---|
| CN202159021U true CN202159021U (en) | 2012-03-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120197659 Expired - Fee Related CN202159021U (en) | 2011-06-13 | 2011-06-13 | Lighting standard board for calibration/detection of chemiluminescent analyzer |
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| CN (1) | CN202159021U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103775951A (en) * | 2012-10-19 | 2014-05-07 | 上海铭源数康生物芯片有限公司 | Light-emitting diode (LED) array based standard plane light source |
| CN107576638A (en) * | 2017-09-28 | 2018-01-12 | 上海瑞纺仪器有限公司 | A kind of Antiultraviolet performance measuring instrument corrector strip |
| CN114076755A (en) * | 2020-08-21 | 2022-02-22 | 湖南省医疗器械检验检测所 | Controllable reference light source for chemiluminescence immunity analyzer |
-
2011
- 2011-06-13 CN CN 201120197659 patent/CN202159021U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103775951A (en) * | 2012-10-19 | 2014-05-07 | 上海铭源数康生物芯片有限公司 | Light-emitting diode (LED) array based standard plane light source |
| CN107576638A (en) * | 2017-09-28 | 2018-01-12 | 上海瑞纺仪器有限公司 | A kind of Antiultraviolet performance measuring instrument corrector strip |
| CN114076755A (en) * | 2020-08-21 | 2022-02-22 | 湖南省医疗器械检验检测所 | Controllable reference light source for chemiluminescence immunity analyzer |
| CN114076755B (en) * | 2020-08-21 | 2024-03-12 | 湖南省医疗器械检验检测所 | Adjustable reference light source for chemiluminescent immunoassay analyzer |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120307 Termination date: 20190613 |
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| CF01 | Termination of patent right due to non-payment of annual fee |