CN202099292U - Filling chamber of filling type bioreactor - Google Patents

Filling chamber of filling type bioreactor Download PDF

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Publication number
CN202099292U
CN202099292U CN 201120213191 CN201120213191U CN202099292U CN 202099292 U CN202099292 U CN 202099292U CN 201120213191 CN201120213191 CN 201120213191 CN 201120213191 U CN201120213191 U CN 201120213191U CN 202099292 U CN202099292 U CN 202099292U
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China
Prior art keywords
bone support
filling
perfusion compartment
housing
perfusion
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Expired - Fee Related
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CN 201120213191
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Chinese (zh)
Inventor
陈维灯
金文玉
姚远
胡庆夕
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University of Shanghai for Science and Technology
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University of Shanghai for Science and Technology
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/46—Means for fastening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
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  • General Engineering & Computer Science (AREA)
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Abstract

The utility model relates to a filling chamber of a filling type bioreactor, which comprises a filling chamber shell and a framework clamp. The filling chamber shell is provided with six fluid inlets and six fluid outlets so that uniformity of fluid flowing speed on cross section of the filling chamber is guaranteed. In the framework clamp, a framework is in interference fit with a framework sealing silicone gasket to be fixedly connected into a round hole at the center of a framework clamping plate, the diameter of the framework is one fourth smaller than that of the filling chamber, and thereby uniformity of flowing speed at a filling inlet of the framework is guaranteed. The framework clamp is mounted into clamping grooves of an upper filling chamber and a lower filling chamber, and sealing performance in filling is guaranteed by means of the sealing silicone gasket. The filling chamber of the filing type bioreactor effectively solves the problem that flowing speed at the section of the inlet of the framework inside the filling chamber of the filling type bioreactor is nonuniform, and is convenient for mounting and demounting of the framework. A high efficiency filling platform is provided for the filling type bioreactor in the bone tissue engineering.

Description

The perfusion compartment of filling type bioreactor
Technical field
The utility model relates to the required key equipment of a kind of filling type bioreactor, particularly a kind of perfusion compartment of filling type bioreactor.Have now in fact and be beneficial to cultivation big section tissue engineered bone.
Background technology
Along with the development of medical technology and the raising of living standards of the people, people are urgent day by day to the requirement of aspects such as damaged reparation of bone and displacement.The various bones that caused by wound, disease are damaged, and especially the reparation of key large segmental bone defect is the difficult problem that present Osteopathic Medicine faces.Solving the damaged ordinary method of bone at present is that bone is transplanted, but limited from body bone bone source, bone xenograft is prone to cause rejection and transmission of disease.The bone defect repair that develops into of bone tissue engineer provides new solution.Osteocyte and bone support carrier are the keys that tissue engineered bone makes up at external co-cultivation environment, also are the focuses of studying both at home and abroad at present.
Bone support training method in bone tissue engineer had static cultivation, revolving bottle formula and stirring-type to cultivate in the past.But in reality was cultivated, these training methods can only make the periphery of bone support grow very thin one deck osseous tissue, and its reason is that these training methods can not guarantee that metabolic waste can not be discharged smoothly to the abundant nutrition supply of bone internal stent osteocyte.The mode of filling type training method through forcing perfusion is when having guaranteed bone internal stent cell sufficient nutrition supplied with; Metabolic waste is discharged smoothly; And can provide suitable hydrodynamic shear to stimulate to cell, promote cell proliferation and differentiation, become the focus of bone tissue engineer research.
Perfusion compartment is one of core component of filling type bioreactor.The design of traditional perfusion compartment directly is placed on the bone support and has many drawbacks in the perfusion compartment of tubular: at first cooperates very difficult assurance sealing property through size between bone support and the perfusion compartment, be prone to cause the directly leakage and influence perfusion effect from the slit between bone support and the perfusion compartment of fluid; Secondly traditional perfusion compartment is very inhomogeneous at the entrance section flow velocity of bone support; Flow velocity is big at the center to be placed on the fluid of bone support at the entrance section place in the perfusion compartment; The edge flow velocity is little, is prone to cause the bone internal stent fluid shearing field of force of perfusion culture inhomogeneous, finally causes the perfusion culture failure; Last pipe perfusion compartment is unfavorable for laying and dismantling of bone support, and the bone support that a kind of perfusion compartment of size can only corresponding a kind of size, if change the size of bone support, just must make the perfusion compartment of correspondingly-sized again.In order at the bone internal stent a uniform mechanics growing environment to be provided for cell, must guarantee the leak free inflow bone of fluid internal stent through the Rational structure of perfusion compartment, guarantee the homogeneity of bone support perfusion entrance section flow velocity simultaneously.
The utility model content
The purpose of the utility model is the defective to the prior art existence, and a kind of perfusion compartment of filling type reactor drum is provided, and can realize the accessibility of bone support installation and removal, realizes the stopping property between perfusion compartment and the bone support, guarantees the perfusion effect.
For achieving the above object, the design of the utility model is: through the analysis that perfusion is required, designed a kind of fluid of realizing at inhomogeneity multiple entry passage of the ingress of bone support perfusion flow velocity and the device for casting that shrinks the perfusion inlet.
According to above-mentioned utility model design, the utility model adopts following technical proposals:
A kind of perfusion compartment of filling type bioreactor: comprise perfusion compartment housing and bone support clamper, it is characterized in that:
(1) said perfusion compartment housing is by upper housing with lower housing is involutory constitutes, and apposition position is lined with silicone gasket, and upper housing is through being bolted on the lower housing;
(2) said bone support clamper is installed in said perfusion compartment housing inner chamber middle part, chamber before and after this inner chamber is divided into;
(3) front end of said perfusion compartment housing has fluid intake, and there is fluid outlet the rear end.
Said silicone gasket is inlaid in the draw-in groove on the involutory flange face of lower housing.
Said bone support clamper is made up of bone support sealed silicon rubber gasket, bone support gripping plate and bone support gripping plate sealing-ring; The bone support of being cultivated is given as security through bone support sealed silicon rubber gasket and is put in the centre hole of bone support gripping plate, makes the sealing between bone support and the bone support gripping plate; Bone support gripping plate is plugged in the ring groove of perfusion compartment housing inner chamber middle part through bone support gripping plate sealing-ring, makes the sealing between bone support gripping plate and the perfusion compartment housing.
The external diameter of said bone support is less than 1/4 of perfusate chamber housing internal diameter.
Said fluid intake and fluid outlet are respectively six tapped fluid intakes and six tapped fluid outlets.
The utility model guarantees dabbling stopping property through the silicone gasket between bone support and perfusion compartment, and the bone support clamping device in six fluid intakes and the perfusion compartment guarantees the homogeneity of fluid at the perfusion ingress of bone support flow velocity.In addition, two parts up and down of perfusion compartment connect with bolt, are convenient to the installation and removal of bone support.
Description of drawings
Fig. 1 is the profile synoptic diagram of an embodiment of the utility model.
Fig. 2 is the explosive view of perfusion compartment housing shown in Figure 1.
Fig. 3 is the bone support clamper structural representation in Fig. 1 perfusate chamber.
Fig. 4 is the structural representation that bone support clamper is installed in down perfusion compartment.
Embodiment
The preferred embodiment of the utility model combines detailed description of the drawings following:
Embodiment 1:Referring to Fig. 1, Fig. 2, Fig. 3 and Fig. 4; The perfusion compartment of this filling type bioreactor comprises perfusion compartment housing and bone support clamper, and said perfusion compartment housing constitutes by upper housing 2 and lower housing 1 are involutory; Apposition position is lined with silicone gasket 4, and upper housing 2 is fixed on the lower housing 1 through bolt 3; Said bone support clamper is installed in said perfusion compartment housing inner chamber middle part, chamber before and after this inner chamber is divided into; The front end of said perfusion compartment housing has fluid intake 9, and there is fluid outlet 10 rear end.
Embodiment 2:Present embodiment and embodiment 1 are basic identical; Special feature is following: said bone support clamper is made up of bone support sealed silicon rubber gasket 6, bone support gripping plate 7 and bone support gripping plate sealing-ring 8; The bone support of being cultivated 5 is given as security through bone support sealed silicon rubber gasket 6 and is put in the centre hole of bone support gripping plate 7, makes the sealing between bone support 5 and the bone support gripping plate; Bone support gripping plate 7 is plugged in the ring groove of perfusion compartment housing inner chamber middle part through bone support gripping plate sealing-ring 8, makes the sealing between bone support gripping plate 7 and the perfusion compartment housing.
Embodiment 3:Present embodiment and embodiment 1 are basic identical; Special feature is following: said perfusion compartment housing is connected through 6 holding bolts 3 with lower housing 1 by upper housing 2; Perfusion compartment sealed silicon rubber gasket 4 through placing between upper housing 2 and the lower housing 1 guarantees its stopping property, forms an airtight perfusion passage.Fluid can flow into from six fluid intakes of said perfusion compartment housing left end, from six fluid outlets outflows of said perfusion compartment housing right-hand member.Six fluid intakes make the fluid uniform mixing in the perfusate chamber, guarantee the homogeneity of said perfusate chamber xsect flow velocity.
Said bone support clamper is as shown in Figure 3: be made up of bone support 5, bone support sealed silicon rubber gasket 6, bone support gripping plate 7 and bone support gripping plate sealring 8.Bone support 5 when passing through shrink-fit installation bone support sealed silicon rubber gasket 6 assurance perfusions in the said bone support clamper between bone support 5 and the bone support gripping plate 7 and the stopping property between the bone support gripping plate 7.
The installation of bone support clamper is as shown in Figure 4: through shrink-fit bone support gripping plate sealed silicon rubber gasket 8 is installed between bone support gripping plate 7 and perfusate chamber housing and is guaranteed stopping property.Runner when bone support clamper is perfusion simultaneously is separated into two parts, forces fluid perfusion to pass through the internal void of bone support 5.Bone support 5 is installed in the central authorities of bone support gripping plate 7, and the external diameter of bone support 5 has avoided the central flow velocity of traditional perfusion compartment big, the little influence to bone support entrance section flow velocity of wall flow velocity all around much smaller than the flow diameter of perfusate chamber.
The utility model pours into the homogeneity of inlet flow velocity for stopping property and the bone support of realizing perfusion compartment, and will help installing and dismounting, is divided into perfusion compartment housing and two parts of bone support clamper to perfusion compartment.The perfusion compartment housing guarantees stopping property by the shrink-fit of upper housing and lower housing two portions and perfusion compartment sealed silicon rubber gasket, and upper housing is connected through bolt with lower housing, helps the installation and removal of interior bone support.Between bone support clamper and the perfusion compartment housing through guaranteeing stopping property with bone support gripping plate sealed silicon rubber gasket shrink-fit.Sealing property when passing through shrink-fit installation bone support sealed silicon rubber gasket assurance perfusion between bone support and the bone support gripping plate can only be poured into fluid and is flow through the bone support.The bone support is installed in the central authorities of bone support gripping plate simultaneously, and the external diameter of bone support has avoided the central flow velocity of traditional perfusion compartment big, the little influence to bone support entrance section flow velocity of wall flow velocity all around less than 1/4 of perfusate chamber perfusion channel diameter.
The utility model following conspicuous advantage arranged: perfusion compartment connects through bolt and goes up lower housing, greatly facilitates the installation and removal of interior bone support.The bone support through and clamper between through shrink-fit the sealed silicon rubber gasket is installed, bone support fluid when perfusion culture can not flowed away from the slit of bone support and bone support gripping plate, guaranteed the perfusion effect, and the easy installation and removal of bone support.Six fluid intakes of perfusate chamber and bone support are installed in the central authorities of perfusion compartment, and the bone stent diameter has guaranteed the homogeneity of bone support perfusion inlet flow velocity less than 1/4 of perfusate chamber diameter.For the filling type bioreactor in the bone tissue engineer provides one to pour into platform efficiently.

Claims (5)

1. the perfusion compartment of a filling type bioreactor: comprise perfusion compartment housing and bone support clamper, it is characterized in that:
A. said perfusion compartment housing is by upper housing (2) and the involutory formation of lower housing (1), and apposition position is lined with silicone gasket (4), and upper housing (2) is fixed on the lower housing (1) through bolt (3);
B. said bone support clamper is installed in said perfusion compartment housing inner chamber middle part, chamber before and after this inner chamber is divided into;
The front end of c. said perfusion compartment housing has fluid intake (9), and there is fluid outlet (10) rear end.
2. the perfusion compartment of filling type bioreactor according to claim 1 is characterized in that said silicone gasket (4) is inlaid in the draw-in groove on the involutory flange face of lower housing (1).
3. the perfusion compartment of filling type bioreactor according to claim 1; It is characterized in that said bone support clamper is made up of bone support sealed silicon rubber gasket (6), bone support gripping plate (7) and bone support gripping plate sealing-ring (8); The bone support of being cultivated (5) is given as security through bone support sealed silicon rubber gasket (6) and is put in the centre hole of bone support gripping plate (7), makes the sealing between bone support (5) and the bone support gripping plate; Bone support gripping plate (7) is plugged in the ring groove of perfusion compartment housing inner chamber middle part through bone support gripping plate sealing-ring (8), makes the sealing between bone support gripping plate (7) and the perfusion compartment housing.
4. the perfusion compartment of filling type bioreactor according to claim 3, the external diameter that it is characterized in that said bone support (5) is less than 1/4 of perfusate chamber housing internal diameter.
5. the perfusion compartment of filling type bioreactor according to claim 1 is characterized in that said fluid intake (9) and fluid outlet (10) are respectively six tapped fluid intakes and six tapped fluid outlets.
CN 201120213191 2011-06-23 2011-06-23 Filling chamber of filling type bioreactor Expired - Fee Related CN202099292U (en)

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Application Number Priority Date Filing Date Title
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103740591A (en) * 2014-01-17 2014-04-23 康珞生物科技（武汉）有限公司 Three-dimensional perfusion type cell culture device and application thereof
US20190330582A1 (en) * 2013-12-30 2019-10-31 New York Stem Cell Foundation, Inc. Perfusion bioreactor

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190330582A1 (en) * 2013-12-30 2019-10-31 New York Stem Cell Foundation, Inc. Perfusion bioreactor
CN103740591A (en) * 2014-01-17 2014-04-23 康珞生物科技（武汉）有限公司 Three-dimensional perfusion type cell culture device and application thereof
CN103740591B (en) * 2014-01-17 2015-07-15 康珞生物科技（武汉）有限公司 Three-dimensional perfusion type cell culture device and application thereof

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C17 Cessation of patent right
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Granted publication date: 20120104

Termination date: 20120623