CN202066862U - Dual-channel detection card capable of simultaneously detecting glibenclamide and gliclazide - Google Patents
Dual-channel detection card capable of simultaneously detecting glibenclamide and gliclazide Download PDFInfo
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- CN202066862U CN202066862U CN2010206765860U CN201020676586U CN202066862U CN 202066862 U CN202066862 U CN 202066862U CN 2010206765860 U CN2010206765860 U CN 2010206765860U CN 201020676586 U CN201020676586 U CN 201020676586U CN 202066862 U CN202066862 U CN 202066862U
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Abstract
The utility model relates to a dual-channel detection card capable of simultaneously detecting glibenclamide and gliclazide, which belongs to the technical field of detection of western medicine constituents illegally added in traditional Chinese medicine. The dual-channel detection card capable of simultaneously detecting glibenclamide and gliclazide is characterized in that: a detection window and a sampling hole are arranged on the surface of a shell of the detection card, and a detecting strip is arranged in the shell; a cellulose nitrate membrane is adhered to the middle of a supporting back plate, a water absorbent membrane is adhered to one end of the supporting back plate, and a sample pad is adhered to the other end of the supporting back plate; two sections of colloidal gold membranes are sandwiched between the cellulose nitrate membrane and the sample pad in an adhering way, the colloidal gold membranes are respectively glass fiber membranes which contain anti-glibenclamide and anti-gliclazide monoclonal antibody colloidal gold markers, and three blot displaying tapes are transversely arranged on the cellulose nitrate membrane at intervals: one tape is a detection tape containing glibenclamide protein conjugates, another tape is a detection tape containing gliclazide protein conjugates, and the third tape is a quality control tape containing anti-rabbit antibodies or anti-mouse antibodies; and the sampling tape is over against the sampling hole, and the cellulose nitrate membrane is over against the detection window. The detection card can simultaneously detect two western medicine constituents illegally added in a hypoglycemic traditional Chinese medicine sample, and has the advantages of detection cost saving, convenience in use, rapid detection, high sensitivity and accurate results.
Description
Technical field
The utility model relates to a kind of illegal utensil that adds western medicine composition in the blood-sugar lowering tcm drug that detects, and particularly relates to a kind of two-channel detection card that detects glibenclamide and gliclazide simultaneously, belongs to Chinese medicine and illegally adds western medicine composition detection technique field.
Background technology
Glibenclamide and gliclazide all belong to the sulfonylurea hypoglycemic agent thing, mainly by promoting that insulin secretion plays a role, in addition, also can strengthen insulin and receptors bind, remove acceptor by the effect of insulin resistance, and insulin action is strengthened.Determined curative effect and cheap, clinical practice is extensive, but also has certain toxic and side effect.Glibenclamide can cause hypoglycemia and other a series of as serious adverse reactions such as hypoadrenocorticism, teratogenesis.Yet some illegal producers mix it in blood-sugar lowering type health-care food in order to strengthen hypoglycemic effect, and the patient is taken under unwitting situation in a large number, cause serious consequence.Therefore set up a kind of analytical approach accurately and reliably, these chemical substances of illegal interpolation are detected be very important.
At present, prior art mainly relies on chromatogram and mass-spectrometric technique for the detection of glibenclamide and gliclazide, and these methods require high to experimental instrument and equipment, and complex operation is high to operating personnel's professional knowledge requirement, wastes time and energy.Therefore setting up a kind of analytical approach that detects Chinese medicine Chinese and Western medicine composition accurately and reliably again quickly and easily is very important.
Summary of the invention
The purpose of this utility model is to provide a kind of utensil that can detect glibenclamide and gliclazide simultaneously, overcomes prior art complex operation, professional demanding defective, is used to investigate and prosecute low-quality goods, guarantees drug safety.
The technical solution of the utility model: a kind of two-channel detection card that detects glibenclamide and gliclazide simultaneously, in the test card shell 1 of rectangular flat shell shape, test-strips 2 is set, the test card case surface has the fenestra 3 of detection and well 4, test-strips is formed by pasting five kinds of elements on the supporting backboard 5: nitrocellulose filter 6 is pasted at supporting backboard middle part, stacked stickup absorbing membrane 10 on supporting backboard one end, stacked stickup sample pad 9 on the other end, absorbing membrane is inner to overlap with nitrocellulose filter one end respectively separately with sample pad the inner, clinch at sample pad and nitrocellulose filter, insert and put between the two and paste two sections collaurum films 7,8, collaurum film 7,8 is the glass fibre membrane that contains anti-glibenclamide monoclonal antibody colloid gold label thing and anti-gliclazide monoclonal antibody colloid gold label thing successively; Three isolated demonstration trace bands are laterally arranged on the nitrocellulose filter: one is that the detection that contains the glibenclamide protein conjugate is with 11, another is that the detection that contains the gliclazide protein conjugate is with 12, the three to be the quality control band 13 that contains anti-rabbit antibody or anti-mouse antibody; Test-strips is inserted in the test card shell, and sample pad is over against well, and nitrocellulose filter is over against detecting fenestra.
The content of glibenclamide and gliclazide in the direct test sample of the method for applied immunology, test sample is blood-sugar lowering tcm drug samples such as capsule, tablet, pill, disposal route is as follows:
(1) a certain amount of sample to be tested of random acquisition is pulverized evenly.
(2) take by weighing sample after 5g pulverizes, add 10mL sample extract, fully mix more than the 5min concussion reaction 30min.
The extract configuration is as follows: add 30mL distilled water in 70mL methyl alcohol.
(3) step (2) gained solution is moved into the 10mL centrifuge tube, the centrifugal 10min of 4000r/min gets the supernatant after centrifugal; If the supernatant clarification is the dilution proportion of 1 ︰ 3 by Shang Qing Ye ︰ sample diluting liquid then, promptly get supernatant 100 μ L and add 300 μ L sample diluting liquids, mix the back and measure; If the supernatant muddiness, then use filter paper filtering, get supernatant after the filtration and add 300 μ L sample diluting liquids by supernatant 100 μ L and dilute, mix the back and measure.
Sample diluting liquid is pH7.6,10mmoL PBST solution.
Detection be with 11,12 and quality control band 13 manifest the brownish red trace simultaneously, the expression testing result is negative, illustrates that glibenclamide and gliclazide all do not exceed standard in the detected sample.
11,12 Show Colors not are with in detection, have only quality control band 13 to manifest the brownish red trace, and testing result is positive, show that glibenclamide and gliclazide all exceed standard in the detected sample.
11 Show Colors not are with in detection, detect be with 12 and quality control band 13 manifest the brownish red trace simultaneously, testing result is positive, show that glibenclamide exceeds standard in the detected sample, and gliclazide does not exceed standard.
12 Show Colors not are with in detection, detect be with 11 and quality control band 13 manifest the brownish red trace simultaneously, testing result is positive, show that gliclazide exceeds standard in the detected sample, and glibenclamide does not exceed standard.
Detection be with 11,12 and quality control band 13 all do not develop the color, show that then detecting strip lost efficacy.
The utility model has the advantages that the glibenclamide and the gliclazide that can detect simultaneously in the blood-sugar lowering tcm drug sample, be easy to preparation and save testing cost, easy to use, detect fast, highly sensitive, the result is accurate.
Description of drawings
The two-channel detection card of Fig. 1 glibenclamide and gliclazide and test-strips arrangement figure in the enclosure.1, shell, 2, test-strips, 3, detect fenestra, 4, well.
The detection strip structural representation of the two-channel detection card of Fig. 2 glibenclamide and gliclazide.5, supporting backboard, 6, nitrocellulose filter, 7, collaurum film (glass fibre membrane that contains anti-glibenclamide monoclonal antibody colloid gold label thing), 8, collaurum film (glass fibre membrane that contains anti-gliclazide monoclonal antibody colloid gold label thing), sample pad 9, absorbing membrane 10.
Fig. 3 detects and shows the trace synoptic diagram on the nitrate glass tunica fibrosa of strip.6, nitrocellulose filter, 11, detect band (containing the glibenclamide protein conjugate), 12, detect band (containing the gliclazide protein conjugate), 13, quality control band.
Embodiment
The utility model further specifies as follows in conjunction with specific embodiments referring to accompanying drawing:
The two-channel detection card of a kind of glibenclamide and gliclazide, it is that test-strips 2 is set in test card shell 1, the setting of the structure of test card shell 1 and test-strips 2 is referring to accompanying drawing 1 and accompanying drawing 2, test card shell 1 is rectangular flat shell shape housing, long 7cm, wide 2cm, thick 0.5cm, shell wall thickness 0.1cm is made by engineering plastics.Test card shell 1 is connected with the shell block two halves by cap and constitutes, and the fenestra 3 of detection and well 4 are arranged on test card shell 1 cap.Test-strips 2 is placed in the shell block of test card shell 1.
Test-strips 2 is fillet thin slices of sandwich construction, and sheet is about 6.0cm, the wide about 0.4cm of sheet, and thickness is less than 2.5mm.Test-strips 2 is sandwich constructions: bottom is a supporting backboard 5, is the igelite thin slice, thick about 0.5mm, long 6.0cm, wide 0.4cm; The stacked stickup nitrocellulose filter 6 in supporting backboard 5 middle parts, cellulose nitrate thickness 0.5mm, long 2-2.5cm, wide 0.4cm.Three isolated horizontal demonstration trace bands are arranged on the nitrocellulose filter 6, referring to accompanying drawing 3, article three, show in the trace band, article one, be to detect to be with 11, contain the glibenclamide protein conjugate, another is to detect to be with 12, contains the gliclazide protein conjugate, article three, be quality control band 13, contain anti-rabbit antibody or anti-mouse antibody.Stacked stickup absorbing membrane 10 on supporting backboard 5 one ends, stacked stickup sample pad 9 on supporting backboard 5 the other end, absorbing membrane 10 the inners overlap with nitrocellulose filter 6 one ends respectively separately with sample pad 9 the inners, at the clinch of sample pad 9 with nitrocellulose filter 6, insert and put between the two and paste two sections collaurum films 7,8, collaurum film 7 is the glass fibre membranes that contain anti-glibenclamide antibody colloidal gold label, and collaurum film 8 is the glass fibre membranes that contain anti-gliclazide antibody colloidal gold label.Collaurum film 7 and collaurum film 8 thick 0.5-0.8mm, long 0.5-0.6cm, wide 0.4cm.Sample pad 9 thick 0.5-0.8mm, long 2cm, wide 0.4cm.Absorbing membrane 10 thick 0.5-0.8mm, long 1.6-2cm, wide 0.4cm.Collaurum film 7,8, sample pad 9 and absorbing membrane 10 are 0.1-0.2cm with the nitrocellulose filter lap of splice.Absorbing membrane 10 materials are thieving paper, and sample pad 9 materials are water adsorption glass tunica fibrosas.
Test-strips 2 is inserted in the shell 1, and sample pad 9 is over against well 4, and nitrocellulose filter 6 is over against detecting fenestra 3.
Test sample is blood-sugar lowering tcm drug samples such as capsule, tablet, pill, and disposal route is as follows:
(1) a certain amount of sample to be tested of random acquisition is pulverized evenly;
(2) take by weighing sample after 5g pulverizes, add 10mL sample extract, fully mix more than the 5min concussion reaction 30min;
The extract configuration is as follows: add 30mL distilled water in 70mL methyl alcohol;
(3) step (2) gained solution is moved into the 10mL centrifuge tube, the centrifugal 10min of 4000r/min gets the supernatant after centrifugal; If the supernatant clarification is the dilution proportion of 1 ︰ 3 by Shang Qing Ye ︰ sample diluting liquid then, promptly get supernatant 100 μ L and add 300 μ L sample diluting liquids, mix the back and measure; If the supernatant muddiness, then use filter paper filtering, get supernatant after the filtration and add 300 μ L sample diluting liquids by supernatant 100 μ L and dilute, mix the back and measure;
Sample diluting liquid is pH7.6,10mmoL PBST solution;
The key reaction of test card is immunologic antigen and antibody response, the antibody of the colloid gold label that on nitrocellulose filter, moves, on calibration tape respectively with contain glibenclamide protein conjugate, gliclazide protein conjugate, and anti-rabbit antibody of quality control band or anti-mouse antibody reaction, form the brownish red band.If have corresponding glibenclamide to be checked and gliclazide all to be higher than permissible value in the sample, after adding, sample promptly reacts with corresponding antibody, and not can with the contained glibenclamide protein conjugate of relevant detection band, the reaction of gliclazide protein conjugate, thereby do not develop the color.The result of key reaction has following 5 kinds of situations:
Detection be with 11,12 and quality control band 13 manifest the brownish red trace simultaneously, the expression testing result is negative, illustrates that glibenclamide and gliclazide all do not exceed standard in the detected sample;
11,12 Show Colors not are with in detection, have only quality control band 13 to manifest the brownish red trace, and testing result is positive, show that glibenclamide and gliclazide all exceed standard in the detected sample;
11 Show Colors not are with in detection, detect be with 12 and quality control band 13 manifest the brownish red trace simultaneously, testing result is positive, show that glibenclamide exceeds standard in the detected sample, and gliclazide does not exceed standard;
12 Show Colors not are with in detection, detect be with 11 and quality control band 13 manifest the brownish red trace simultaneously, testing result is positive, show that gliclazide exceeds standard in the detected sample, and glibenclamide does not exceed standard;
Detection be with 11,12 and quality control band 13 all do not develop the color, show that then detecting strip lost efficacy.
Claims (1)
1. a two-channel detection card that detects glibenclamide and gliclazide simultaneously is characterized in that in the test card shell (1) of rectangular flat shell shape test-strips (2) being set, and the test card case surface has detection fenestra (3) and well (4); Test-strips goes up five kinds of elements of stickup by supporting backboard (5) and forms: nitrocellulose filter (6) is pasted at supporting backboard middle part, stacked stickup absorbing membrane (10) on supporting backboard one end, stacked stickup sample pad (9) on the other end, absorbing membrane is inner to overlap with nitrocellulose filter one end respectively separately with sample pad the inner, clinch at sample pad and nitrocellulose filter, insert and put between the two and paste two sections collaurum films (7), (8), collaurum film (7), (8) be to contain anti-glibenclamide monoclonal antibody colloid gold label thing successively, the glass fibre membrane of anti-gliclazide monoclonal antibody colloid gold label thing; Three isolated demonstration trace bands are laterally arranged on the nitrocellulose filter: one is the detection band (11) that contains the glibenclamide protein conjugate, another is the detection band (12) that contains the gliclazide protein conjugate, and the 3rd is the quality control band (13) that contains anti-rabbit antibody or anti-mouse antibody; Test-strips is inserted in the test card shell, and sample pad is over against well, and nitrocellulose filter is over against detecting fenestra.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010206765860U CN202066862U (en) | 2010-12-23 | 2010-12-23 | Dual-channel detection card capable of simultaneously detecting glibenclamide and gliclazide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010206765860U CN202066862U (en) | 2010-12-23 | 2010-12-23 | Dual-channel detection card capable of simultaneously detecting glibenclamide and gliclazide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202066862U true CN202066862U (en) | 2011-12-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010206765860U Expired - Fee Related CN202066862U (en) | 2010-12-23 | 2010-12-23 | Dual-channel detection card capable of simultaneously detecting glibenclamide and gliclazide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202066862U (en) |
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2010
- 2010-12-23 CN CN2010206765860U patent/CN202066862U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111207 Termination date: 20171223 |