CN202033515U - Microscope applicable to low-temperature environment - Google Patents
Microscope applicable to low-temperature environment Download PDFInfo
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- CN202033515U CN202033515U CN201120093510XU CN201120093510U CN202033515U CN 202033515 U CN202033515 U CN 202033515U CN 201120093510X U CN201120093510X U CN 201120093510XU CN 201120093510 U CN201120093510 U CN 201120093510U CN 202033515 U CN202033515 U CN 202033515U
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- groove milling
- hole
- low temperature
- objective table
- pipeline
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Abstract
The utility model discloses a microscope applicable to a low-temperature environment. The microscope comprises a nitrogen bottle, a pressure reducing valve, a drying device, a flow meter, a low-temperature objective table, an objective lens and a spotlight, wherein the low-temperature objective table is partitioned into four layers by upper layer glass, double-layer glass consisting of cover glass and a glass slide and lower layer glass; the flow meter is connected with a heat exchanger and a first gas distributing head through pipelines in sequence; nitrogen enters the low-temperature objective table after the temperature of the nitrogen is adjusted through a temperature adjusting chamber, is used for cooling a sample and discharged, is heated to be above a dew-point temperature of ambient air through a rewarming chamber and is discharged to one side of the upper layer glass and the lower layer glass which are contacted with air. In the microscope, dewing is prevented by using rewarming of the discharged nitrogen, so that waste nitrogen is reutilized; and the device has a compact structure, is convenient to operate and has an obvious anti-dewing and anti-frosting effect.
Description
Technical field
The utility model relates to the low-temperature biological medical domain, particularly relates to a kind of microscope that is applicable to low temperature environment.
Background technology
The microscope that is applicable to low temperature environment is widely used in the low-temperature biological medical domain, it mainly is the situation of change by various cells of the microscopic examination that is applicable to low temperature environment and biological tissue under low temperature environment, now, people have crossed the formation of the inside and outside ice crystal of cell with the microscope direct observing that is applicable to low temperature environment, the variation of cell appearance size etc., and a large amount of important information materials have been obtained, thereby can qualitative, quantitative ground analysis and research low temperature to the influence of subjects, greatly promoted the development of low-temperature biological medical domain, the obtaining of these achievements further recognized people to be applicable to the microscopical importance of low temperature environment.
The microscope that is applicable to low temperature environment of experience development decades, though brought a lot of gratifying achievements, but the microscope that is applicable to low temperature environment still exists deficiency, the anti-condensation problem of low temperature objective table particularly, in case glass sheet Mechanism of External Condensation of Vapor under observation, influenced passing through of visible light, observation will not gone down.Previous design is that this texture ratio is heavy, and takes up space very much, uses also very inconvenience with whole cover such as spotlight, low temperature objective table and object lens, and cost is than higher, and causes the waste of nitrogen.Some semi-enclosed objective table structures appear again in the later stage, be that the nitrogen that objective table is discharged is covered the glassy layer surface, but the nitrogen that temperature is lower can make airborne water vapor be liquefied as the globule to be dripped on glassly, can influence light path equally and pass through, and the outlet of cold nitrogen can produce frosting.
Summary of the invention
The purpose of this utility model is to overcome above-mentioned the deficiencies in the prior art, and a kind of microscope that is applicable to low temperature environment is provided, and solve long-time observation of cell or tissue variation under low temperature environment, and the Mechanism of External Condensation of Vapor that causes causes observing the problem that can't carry out.
Technical solution of the present utility model is as follows:
A kind of microscope that is applicable to low temperature environment, comprise nitrogen cylinder, be connected by pipeline with flowmeter through reduction valve, drying device, it is characterized in that, also comprise the low temperature objective table, be positioned at the object lens of this low temperature objective table below and be positioned at the spotlight of this low temperature objective table top;
The framework of described low temperature objective table is made of heat-barrier material, be " [" shape, be provided with the first parallel groove milling on this frame inner wall from top to bottom successively, second groove milling and the 3rd groove milling, upper strata glass embeds this first groove milling, the double glazing that cover glass and microslide constitute embeds this second groove milling, lower floor's glass embeds the 3rd groove milling, be respectively equipped with first hole and the 3rd hole on low temperature objective table front side frame between this first groove milling and second groove milling and the rear frame, be respectively equipped with second hole and the 4th hole on low temperature objective table front side frame between this second groove milling and the 3rd groove milling and the rear frame, with described the 3rd hole and and first groove milling of the 4th hole homonymy top and the 3rd groove milling below the low temperature stage frame on be respectively equipped with the 5th hole and the 6th hole, have the groove milling of vertical direction on described low temperature objective table front side frame and the rear frame inner wall outside, embed for transparent baffle;
Described flowmeter connects heat interchanger successively by pipeline, the tempering room and first gas separating port, described heat interchanger places liquid nitrogen container, this pipeline is divided into first pipeline and second pipeline through described first gas separating port, the other end of this first pipeline communicates with described first hole, the other end of this second pipeline communicates with described second hole, described the 3rd hole links to each other with the first thick gas outlet, described the 4th hole links to each other with the second thick gas outlet, the other end of this first thick gas outlet and the second thick gas outlet is respectively with after the rewarming chamber is connected, through second gas separating port, be divided into the first thin gas piping and the second thin gas piping, the other end of this first thin gas piping and the second thin gas piping communicates with described the 5th hole and the 6th hole respectively.
The thickness of the thickness of described first groove milling and described upper strata glass is suitable, and the thickness degree of described second groove milling and the thickness of described double glazing are suitable, and the thickness of the thickness of described the 3rd groove milling and described lower floor glass is suitable.
Compared with prior art, the beneficial effects of the utility model are:
(1) the rewarming chamber nitrogen of discharging is through being heated to more than the water vapor dewpoint temperature, and then from the upper and lower glass and the discharge of air contact side, that side Yin Wendu that has avoided contacting with air is lower than dewpoint temperature and the dewfall frosting is used very reliable.
(2) compare with the device that spotlight, objective table and object lens are all covered, the utility model nitrogen consumption significantly reduces, and saves a large amount of nitrogen.
(3) the utility model compact conformation is small and exquisite, and is easy to operate, and big or small laboratory is all configurable.
Description of drawings
Fig. 1 is the microscopical structural representation that the utility model is applicable to low temperature environment.
Fig. 2 is the structural representation that the utility model is applicable to low temperature objective table in the microscope of low temperature environment.
Among the figure: 1. nitrogen cylinder, 2. reduction valve, 3. drying device, 4. flowmeter, 5. heat interchanger, 6. liquid nitrogen container, 7. tempering room, 8 first gas separating ports, 9. spotlight, 101. first thin gas pipings, 102. second thin gas pipings, 11. second gas separating ports rewarming chambers 12., 13. the low temperature objective table, 14. object lens, 151. first thick gas outlets, 152. second thick gas outlets, 131. the low temperature stage frame, 132. upper strata glass, 133. cover glasses, 134. microslide, 135. lower floor's glass, 136. transparent baffles.
Embodiment
The utility model is described in further detail below in conjunction with the drawings and specific embodiments, but should not limit protection domain of the present utility model with this.
Please consult Fig. 1 earlier, Fig. 1 is the microscopical structural representation that the utility model is applicable to low temperature environment.As shown in the figure, a kind of microscope that is applicable to low temperature environment, comprise nitrogen cylinder 1, be connected by pipeline through reduction valve 2, drying device 3 and flowmeter 4, be characterized in, also comprise low temperature objective table 13, be positioned at the object lens 14 of these low temperature objective table 13 belows and be positioned at the spotlight 9 of these low temperature objective table 13 tops; Described flowmeter 4 connects heat interchanger 5 successively by pipeline, the tempering room 7 and first gas separating port 8, described heat interchanger 5 places liquid nitrogen container, this pipeline is divided into first pipeline and second pipeline through this first gas separating port 8, this first pipeline other end communicates with described first hole, the second pipeline other end communicates with described second hole, described the 3rd hole links to each other with the first thick gas outlet 151, described the 4th hole links to each other with the second thick gas outlet 152, the other end of this first thick gas outlet 151 and the second thick gas outlet 152 is respectively with after rewarming chamber 12 is connected, through second gas separating port 11, be divided into the first thin gas piping 101 and the second thin gas piping 102, the other end of this first thin gas piping 101 and the second thin gas piping 102 communicates with described the 5th hole and the 6th hole respectively.
Fig. 2 is the structural representation that the utility model is applicable to low temperature objective table in the microscope of low temperature environment, as shown in the figure, the framework 131 of described low temperature objective table is made of heat-barrier material, be [shape, be provided with the first parallel groove milling on this frame inner wall from top to bottom successively, second groove milling and the 3rd groove milling, upper strata glass embeds this first groove milling, the double glazing that cover glass 133 and microslide 134 constitute embeds this second groove milling, lower floor's glass embeds the 3rd groove milling, be respectively equipped with first hole and the 3rd hole on low temperature objective table front side frame between this first groove milling and second groove milling and the rear frame, be respectively equipped with second hole and the 4th hole on low temperature objective table front side frame between this second groove milling and the 3rd groove milling and the rear frame, with described the 3rd hole and and first groove milling of the 4th hole homonymy top and the 3rd groove milling below the low temperature stage frame on be respectively equipped with the 5th hole and the 6th hole, have the groove milling of vertical direction on described low temperature objective table front side frame and the rear frame inner wall outside, embed for transparent baffle 136; The thickness of the thickness of described first groove milling and described upper strata glass is suitable, and the thickness degree of described second groove milling and the thickness of described double glazing are suitable, and the thickness of the thickness of described the 3rd groove milling and described lower floor glass is suitable.
Specific implementation of the present utility model is that nitrogen in the nitrogen cylinder 1 is after reduction valve 2 decompression, by drying device 3, flowmeter 4, enter the heat interchanger 5 that is immersed in the liquid nitrogen bottle 6 then, normal temperature nitrogen and liquid nitrogen heat exchange wherein, be cooled to low temperature nitrogen, low temperature nitrogen needs temperature adjustment in tempering room 7 before entering low temperature objective table 13, make nitrogen reach the temperature of former setting, be divided into the two-way low temperature nitrogen through first gas separating port 8 and enter low temperature objective table 13 respectively, the cooling sample, after sample temperature is descended rapidly, discharge from the other end pipeline of low temperature objective table 13, the nitrogen of discharging from low temperature objective table 13 is heated to more than the dewpoint temperature of surrounding air through rewarming chamber 12, then, be disposed to the upper strata glass 132 that contacts with air and a side of lower floor's glass 135 respectively, form the two-layer covering nitrogen film of glass sheet up and down by 11 fens two-way of second gas separating port, because it is last, the nitrogen of lower floor's glass contact is through being heated above dewpoint temperature, so but nitrogen film anti-condensation frosting.
Through verification experimental verification, the rewarming again that nitrogen is discharged in the utility model utilization prevents dewfall, and useless nitrogen is reused, and the apparatus structure compactness, and easy to operate, anti-condensation frosting effect is obvious, and low-temperature biological laboratory etc. are all configurable.
Claims (2)
1. microscope that is applicable to low temperature environment, comprise nitrogen cylinder (1), be connected by pipeline with flowmeter (4) through reduction valve (2), drying device (3), it is characterized in that, also comprise low temperature objective table (13), be positioned at the object lens (14) of this low temperature objective table (13) below and be positioned at spotlight (9) above this low temperature objective table (13);
The framework of described low temperature objective table (131) is made of heat-barrier material, be [shape, be provided with the first parallel groove milling on this frame inner wall from top to bottom successively, second groove milling and the 3rd groove milling, upper strata glass embeds this first groove milling, the double glazing that cover glass (133) and microslide (134) constitute embeds this second groove milling, lower floor's glass embeds the 3rd groove milling, be respectively equipped with first hole and the 3rd hole on low temperature objective table front side frame between this first groove milling and second groove milling and the rear frame, be respectively equipped with second hole and the 4th hole on low temperature objective table front side frame between this second groove milling and the 3rd groove milling and the rear frame, with described the 3rd hole and and first groove milling of the 4th hole homonymy top and the 3rd groove milling below the low temperature stage frame on be respectively equipped with the 5th hole and the 6th hole, have the groove milling of vertical direction on described low temperature objective table front side frame and the rear frame inner wall outside, embed for transparent baffle (136);
Described flowmeter (4) connects heat interchanger (5) successively by pipeline, tempering room (7) and first gas separating port (8), described heat interchanger (5) places liquid nitrogen container, this pipeline is divided into first pipeline and second pipeline through this first gas separating port (8), this first pipeline other end communicates with described first hole, the second pipeline other end communicates with described second hole, described the 3rd hole links to each other with the first thick gas outlet (151), described the 4th hole links to each other with the second thick gas outlet (152), the other end of this first thick gas outlet (151) and the second thick gas outlet (152) is respectively with after rewarming chamber (12) are connected, through second gas separating port (11), be divided into the first thin gas piping (101) and the second thin gas piping (102), the other end of this first thin gas piping (101) and the second thin gas piping (102) communicates with described the 5th hole and the 6th hole respectively.
2. according to the described microscope that is applicable to low temperature environment of claim, the thickness that it is characterized in that the thickness of described first groove milling and described upper strata glass is suitable, the thickness degree of described second groove milling and the thickness of described double glazing are suitable, and the thickness of the thickness of described the 3rd groove milling and described lower floor glass is suitable.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201120093510XU CN202033515U (en) | 2011-04-01 | 2011-04-01 | Microscope applicable to low-temperature environment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201120093510XU CN202033515U (en) | 2011-04-01 | 2011-04-01 | Microscope applicable to low-temperature environment |
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| Publication Number | Publication Date |
|---|---|
| CN202033515U true CN202033515U (en) | 2011-11-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201120093510XU Expired - Lifetime CN202033515U (en) | 2011-04-01 | 2011-04-01 | Microscope applicable to low-temperature environment |
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| Country | Link |
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| CN (1) | CN202033515U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102147522A (en) * | 2011-04-01 | 2011-08-10 | 上海理工大学 | Low-temperature microscope |
| CN102692495A (en) * | 2012-06-14 | 2012-09-26 | 中国科学技术大学 | Perfusion microscope |
-
2011
- 2011-04-01 CN CN201120093510XU patent/CN202033515U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102147522A (en) * | 2011-04-01 | 2011-08-10 | 上海理工大学 | Low-temperature microscope |
| CN102692495A (en) * | 2012-06-14 | 2012-09-26 | 中国科学技术大学 | Perfusion microscope |
| CN102692495B (en) * | 2012-06-14 | 2013-12-11 | 中国科学技术大学 | Perfusion microscope |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20111109 Effective date of abandoning: 20120704 |