CN204514856U - The special electrophoresis tank of a kind of comet - Google Patents
The special electrophoresis tank of a kind of comet Download PDFInfo
- Publication number
- CN204514856U CN204514856U CN201520206405.0U CN201520206405U CN204514856U CN 204514856 U CN204514856 U CN 204514856U CN 201520206405 U CN201520206405 U CN 201520206405U CN 204514856 U CN204514856 U CN 204514856U
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- China
- Prior art keywords
- tank
- slide rack
- overcoat
- comet
- electrophoresis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000001962 electrophoresis Methods 0.000 title claims abstract description 39
- 239000007788 liquid Substances 0.000 claims abstract description 37
- 239000012530 fluid Substances 0.000 claims abstract description 24
- 238000007599 discharging Methods 0.000 claims abstract description 10
- 238000010276 construction Methods 0.000 claims abstract description 5
- 238000001704 evaporation Methods 0.000 claims description 8
- 230000008020 evaporation Effects 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000002474 experimental method Methods 0.000 abstract description 8
- 241000196324 Embryophyta Species 0.000 description 16
- 210000004027 cell Anatomy 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000009089 cytolysis Effects 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 230000005778 DNA damage Effects 0.000 description 2
- 231100000277 DNA damage Toxicity 0.000 description 2
- 238000005336 cracking Methods 0.000 description 2
- 238000013016 damping Methods 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 238000013508 migration Methods 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 238000010079 rubber tapping Methods 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000003927 comet assay Methods 0.000 description 1
- 231100000170 comet assay Toxicity 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 229920006351 engineering plastic Polymers 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 210000000633 nuclear envelope Anatomy 0.000 description 1
- 238000000197 pyrolysis Methods 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- -1 when electrophoresis Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of special electrophoresis tank that can complete whole comet, the technical scheme adopted is: tank coat suit is in the outside of tank, cover plate is movably arranged on the top of tank, for shutting out the light, refrigerating plant is arranged between tank and tank overcoat, two electrode pairs should be arranged on the left and right sides in tank, electrophoresis apparatus is arranged on the outside of tank overcoat, two electrodes are all electrically connected with electrophoresis apparatus, fluid filling pump is arranged on the outside of tank overcoat, bottom of gullet is provided with inlet and liquid outlet, inlet is connected with the liquid-feeding tube of fluid filling pump, liquid outlet is connected with discharging tube, discharging tube is provided with valve, slide rack is movably arranged in tank, slide rack is rectangular parallelepiped frame-shaped construction, the corresponding aperture having one or more layers horizontal distribution on slide rack side plate, little hole horizontal inserts hack lever, slide rack is separated into some layers by hack lever, to place the microslide being covered with cell, the utility model can be widely used in molecular biosciences experiment field.
Description
Technical field
The special electrophoresis tank of a kind of comet of the utility model, belongs to molecular biosciences experimental technique field.
Background technology
Comet (Comet assay) is a kind of technology of Sensitive Detection individual cells DNA damage, the cell on microslide is adhered to by Ago-Gel, under the effect of lysate, cell membrane, nuclear membrane and other biological membrane are destroyed, other composition in cell except core DNA is diffused in lysate, when electrophoresis, core DNA rests on original position greatly because of its molecular weight, and the DNA part anode migration of different size, be formed in the conditions of streaking of fluorescence microscopy Microscopic observation likeness in form comet, carry out the degree of researching DNA damage according to the migration of DNA in agarose.Because comet has simple, quick, sensitive feature, be widely used in radiobiology (Gu Yuehua, 2006), biological monitoring (Collins A at present
et al., 1997), the research field such as genetoxic study mechanism (Cheng Xiaojie, 2008).Comet process need carries out under 4 DEG C of constant temperatures, avoids the electrophoretic buffer when low-voltage electrophoresis constantly to produce heat, affects experimental result; Illumination will be prevented, to avoid ultraviolet to the additional injuries of DNA simultaneously.But at present without Special experimental equipment, electrophoresis tank, splitter are positioned in refrigerator and complete by common way, but be also not quite similar due to the temperature of each position of refrigerator, different time, the repeated result that can convince cannot be obtained.In addition in whole experimentation, need in different vessels, complete the steps such as lysis, electrophoresis, washing, because hydraulic shock causes the softening and movement of Ago-Gel on microslide, finally cause coming unstuck, the failure of an experiment rate is high, and existing electrophoresis tank can to place experiment sample amount few, generally can only carry 4 microslides, experiment comparability is poor, and workload is large.
Utility model content
The utility model overcomes the deficiency that prior art exists, and provides a kind of special electrophoresis tank that can complete whole comet.
In order to solve the problems of the technologies described above, the technical solution adopted in the utility model is: the special electrophoresis tank of a kind of comet, comprise tank, electrode, slide rack, refrigerating plant, electrophoresis apparatus, fluid filling pump, cover plate and tank overcoat, described tank coat suit is in the outside of tank, described cover plate is movably arranged on the top of tank, for shutting out the light, described refrigerating plant is arranged between tank and tank overcoat, electrode pair described in two should be arranged on the left and right sides in tank, described electrophoresis apparatus is arranged on the outside of tank overcoat, two described electrodes are all electrically connected with electrophoresis apparatus, described fluid filling pump is arranged on the outside of tank overcoat, liquid feeding in described fluid filling pump water supply groove, described bottom of gullet is provided with inlet and liquid outlet, described inlet is connected with the liquid-feeding tube of fluid filling pump, described liquid outlet is connected with discharging tube, described discharging tube is provided with valve, described slide rack is movably arranged in tank, described slide rack is rectangular parallelepiped frame-shaped construction, the corresponding aperture having one or more layers horizontal distribution on described slide rack side plate, described aperture is used for level and inserts hack lever, slide rack is separated into some layers by described hack lever, to place the microslide being covered with cell.
Preferably, the structure of described refrigerating plant is: comprise evaporation tube, and described evaporation tube is around being arranged on sink outer walls surrounding.
Preferably, temperature sensor is provided with between described tank and tank overcoat.
Preferably, described tank overcoat arranged outside has shell.
Preferably, described outer side is vertically provided with liquid level tube, and the lower end of described liquid level tube is communicated with, to observe the height of liquid level in tank with below in tank.
Preferably, described slide rack is provided with handle, so that slide rack is taken out or put in tank.
The beneficial effect that the utility model compared with prior art has is: the utility model not only meets the low temperature required by comet, the conditions such as lucifuge, and in the tank closed, once can complete the cracking of comet, washing, untwist, electrophoresis, multiple links such as neutralization, simultaneously, owing to have employed fluid filling pump liquid feeding, the beginning of liquid feeding can be controlled, stop, control flow, and liquid passes in and out from tank bottom, ensure that microslide does not move, do not tilt, Ago-Gel on such microslide is with regard to difficult drop-off, improve experiment success rate, accurately can control liquid level simultaneously, experimental implementation is simplified.In addition the utility model is owing to have employed slide rack, and one time experiment sample microslide number ratio prior art improves several times, and microslide is placed, it is convenient, fast to extract, and conventional efficient promotes greatly.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the utility model is described further.
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the structural representation of refrigerating plant in the utility model.
Fig. 3 is the structural representation of slide rack in the utility model.
Fig. 4 is the vertical view of slide rack in the utility model.
In figure: 1 be tank, 2 be electrode, 3 be slide rack, 4 be refrigerating plant, 41 be evaporation tube, 5 be electrophoresis apparatus, 6 be fluid filling pump, 7 be cover plate, 8 be tank overcoat, 9 be shell, 10 for liquid level tube.
Embodiment
As shown in Figure 1 to 4, the special electrophoresis tank of a kind of comet of the utility model, comprise tank 1, electrode 2, slide rack 3, refrigerating plant 4, electrophoresis apparatus 5, fluid filling pump 6, cover plate 7 and tank overcoat 8, described tank overcoat 8 is sleeved on the outside of tank 1, described cover plate 7 is movably arranged on the top of tank 1, for shutting out the light, described refrigerating plant 4 is arranged between tank 1 and tank overcoat 8, electrode 2 correspondence described in two is arranged on the left and right sides in tank 1, described electrophoresis apparatus 5 is arranged on the outside of tank overcoat 8, two described electrodes 2 are all electrically connected with electrophoresis apparatus 5, described fluid filling pump 6 is arranged on the outside of tank overcoat 8, liquid feeding in described fluid filling pump 6 water supply groove 1, inlet and liquid outlet is provided with bottom described tank 1, described inlet is connected with the liquid-feeding tube of fluid filling pump 6, described liquid outlet is connected with discharging tube, described discharging tube is provided with valve, described slide rack 3 is movably arranged in tank 1, described slide rack 3 is rectangular parallelepiped frame-shaped construction, the corresponding aperture having one or more layers horizontal distribution on described slide rack 3 side plate, described aperture is used for level and inserts hack lever, slide rack 3 is separated into some layers by described hack lever, to place the microslide being covered with cell.
The structure of described refrigerating plant 4 is: comprise evaporation tube 41, and described evaporation tube 41 is around being arranged on tank 1 outer wall surrounding.
Temperature sensor is provided with between described tank 1 and tank overcoat 8.
Described tank overcoat 8 arranged outside has shell 9.
Described outer side is vertically provided with liquid level tube 10, and the lower end of described liquid level tube is communicated with, to observe the height of liquid level in tank 1 with below in tank 1.
Described slide rack 3 is provided with handle, so that slide rack 3 is taken out or put in tank 1.
The utility model is in the outer wall surrounding of tank 1 around the evaporation tube 41 of refrigerating plant 4, and refrigerating plant 4 is arranged in tank overcoat 8; Fill water between tank overcoat 8 and tank 1 outer wall during use, temperature sensor is arranged on wherein, and refrigerating plant 4 runs, and makes the constant temperature in setting of the fluid temperature between tank overcoat 8 and tank 1; Two electrodes 2 are fixedly mounted on the left and right sides in tank 1, and with electrophoresis apparatus 5 Electrode connection, electrophoresis apparatus 5 is arranged on outside tank overcoat 8, and refrigerating plant 4 main frame is arranged on outside tank overcoat 8; Fluid filling pump 6 is arranged on outside tank overcoat 8, and inlet is connected with liquid-feeding tube, and liquid outlet enters a side lower part in tank 1 by pipeline; In tank 1, the discharging tube of opposite side bottom tapping valve connects, and discharging tube leads to outside shell; All devices are installed in shell 9 above.Liquid level tube 10 is vertically mounted on outside shell 9, and liquid level tube 10 lower end and the interior below of tank 1 are connected, to observe the height of liquid level; Slide rack 3 is rectangular parallelepiped frame-shaped construction, and side plate has the aperture of 4 layers of horizontal distribution, and hack lever level can insert aperture, and hack lever forms some layers as required, experimentally needs above to place the microslide being covered with cell; Slide rack 3 top is provided with handle; During use, slide rack 3 is put in the middle part of tank 1; The cover plate 7 of black can cover on tank 1 top, above handle is housed, is provided with the aperture of slotting thermometer.
Use step of the present utility model: in advance the lysis liquid container that experiment uses is put into refrigerator, be chilled to 4 DEG C in advance, open the utility model refrigerating plant 4 power supply, regulate the temperature controller of refrigerating plant 4, make the water temperature in tank overcoat 8 be stabilized in 4 DEG C in whole experimentation.Slide rack 3(is put in the microslide layering being covered with cell made and is plugged hack lever as required, 4 layers can be put at most, 5 ~ 7 every layer), put in the middle part of tank 1, the external liquid feeding mouth of pipe is inserted in the lysis liquid container of precooling, open fluid filling pump 6, liquid is inhaled into tank 1, observes liquid level tube 10, liquid level is made just not had gel on microslide, close fluid filling pump 6,4 DEG C of constant temperature, cracking 2 hours; Open tapping valve, discharge cell pyrolysis liquid; Similar with above-mentioned steps, then add the distilled water of precooling, do not have microslide, washed 3 times, 5 min/ time; After washing terminates, add the alkaline electrophoresis buffer of precooling, damping fluid height was as the criterion not have the gel on microslide, unwindase 13 0 minute; Open electrophoresis apparatus 5 switch, regulation voltage is 23 V, electric current be 300 mA(can experimentally situation regulate), 4 DEG C of constant temperature, electrophoresis 40 min; Bleed off damping fluid, add distilled water, wash 1 time, about 10 min; After bleeding off distilled water, take out slide rack 3, blot the moisture on microslide with filter paper, observation of just can dyeing.In experimentation, during slide rack 3 puts into tank 1 to taking-up, the cover plate 7 of black covers all the time on tank 1, irradiates to prevent light.Thermometer cover plate 1 inserted in tank 1 can observe fluid temperature in tank 1 in time, when temperature is higher than 4 DEG C, and adjustable temperature controller temperature or manually boot refrigerating plant 4 with regulates liquid temperature.
In the utility model, refrigerating plant 4 and temperature-controlled member thereof are prior art, adopt compressor, freon refrigeration, (semiconductor cooling device also can be adopted to freeze).Electrode and install identical with existing electrophoresis tank technology, electrophoresis apparatus 5 is prior art, and fluid filling pump 6 is prior art, employing finished product miniwatt micro pump.The material of the material of tank 1, the material of slide rack 3, cover plate 7 is insulation, light-proof material, and the utility model miscellaneous part adopts black ABS engineering plastics to make.
The utility model is particularly suitable for comet, improves a little, also can be widely used in the biochemical test such as various electrophoresis, electrolysis, ionization needing constant temperature.
By reference to the accompanying drawings embodiment of the present utility model is explained in detail above, but the utility model is not limited to above-described embodiment, in the ken that those of ordinary skill in the art possess, various change can also be made under the prerequisite not departing from the utility model aim.
Claims (6)
1. the special electrophoresis tank of comet, it is characterized in that, comprise tank (1), electrode (2), slide rack (3), refrigerating plant (4), electrophoresis apparatus (5), fluid filling pump (6), cover plate (7) and tank overcoat (8), described tank overcoat (8) is sleeved on the outside of tank (1), described cover plate (7) is movably arranged on the top of tank (1), for shutting out the light, described refrigerating plant (4) is arranged between tank (1) and tank overcoat (8), electrode (2) correspondence described in two is arranged on the left and right sides in tank (1), described electrophoresis apparatus (5) is arranged on the outside of tank overcoat (8), two described electrodes (2) are all electrically connected with electrophoresis apparatus (5), described fluid filling pump (6) is arranged on the outside of tank overcoat (8), described fluid filling pump (6) water supply groove (1) interior liquid feeding, described tank (1) bottom is provided with inlet and liquid outlet, described inlet is connected with the liquid-feeding tube of fluid filling pump (6), described liquid outlet is connected with discharging tube, described discharging tube is provided with valve, described slide rack (3) is movably arranged in tank (1), described slide rack (3) is rectangular parallelepiped frame-shaped construction, the corresponding aperture having one or more layers horizontal distribution on described slide rack (3) side plate, described aperture is used for level and inserts hack lever, slide rack (3) is separated into some layers by described hack lever, to place the microslide being covered with cell.
2. the special electrophoresis tank of a kind of comet according to claim 1, is characterized in that, the structure of described refrigerating plant (4) is: comprise evaporation tube (41), and described evaporation tube (41) is around being arranged on tank (1) outer wall surrounding.
3. the special electrophoresis tank of a kind of comet according to claim 1 and 2, is characterized in that, is provided with temperature sensor between described tank (1) and tank overcoat (8).
4. the special electrophoresis tank of a kind of comet according to claim 1 and 2, is characterized in that, described tank overcoat (8) arranged outside has shell (9).
5. the special electrophoresis tank of a kind of comet according to claim 4, is characterized in that, described outer side is vertically provided with liquid level tube (10), and the lower end of described liquid level tube is communicated with, to observe the height of tank (1) interior liquid level with below in tank (1).
6. the special electrophoresis tank of a kind of comet according to claim 1, is characterized in that, (3) are provided with handle to described slide rack, so that slide rack (3) is taken out or put in tank (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520206405.0U CN204514856U (en) | 2015-04-08 | 2015-04-08 | The special electrophoresis tank of a kind of comet |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520206405.0U CN204514856U (en) | 2015-04-08 | 2015-04-08 | The special electrophoresis tank of a kind of comet |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204514856U true CN204514856U (en) | 2015-07-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520206405.0U Expired - Fee Related CN204514856U (en) | 2015-04-08 | 2015-04-08 | The special electrophoresis tank of a kind of comet |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204514856U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107034510A (en) * | 2017-05-06 | 2017-08-11 | 哈尔滨工业大学 | Carbon fiber surface depositing noble metal electrophoretic deposition set |
-
2015
- 2015-04-08 CN CN201520206405.0U patent/CN204514856U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107034510A (en) * | 2017-05-06 | 2017-08-11 | 哈尔滨工业大学 | Carbon fiber surface depositing noble metal electrophoretic deposition set |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160408 Address after: 046011 73 North East Street, Changzhi University, Shanxi, Changzhi Patentee after: Changzhi University Address before: 046011 No. 73 North East Street, Changzhi City, Shanxi Province Patentee before: Bian Gaopeng |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150729 Termination date: 20170408 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |