CN202002827U - Air microbial sampling system - Google Patents
Air microbial sampling system Download PDFInfo
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- CN202002827U CN202002827U CN201020678465XU CN201020678465U CN202002827U CN 202002827 U CN202002827 U CN 202002827U CN 201020678465X U CN201020678465X U CN 201020678465XU CN 201020678465 U CN201020678465 U CN 201020678465U CN 202002827 U CN202002827 U CN 202002827U
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- nozzle
- escape pipe
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Abstract
The utility model relates to an air microbial sampling system, which adopts an inhaled vortex water filtration principle to conduct enrichment for microorganisms in a certain volume of air in a certain period of time through a liquid filtration way, the bottom end of a nozzle is provided with an impact hole so as to form more smaller air bubbles of gas through the utilizing of jet stream, a diversion part is provided with a cyclone hole, so that high-speed flow of air can be utilized to push the collected liquid to form the vortex to prompt mutual extruding among air bubbles, so as to increase the contact area between air and liquid enrichment and increase the collecting efficiency of microorganisms.
Description
[technical field]
The utility model relates to a kind of microbe sampling system, particularly a kind of air microbe sampling system.
[background technology]
Microorganism plays important effect as participating in material round-robin important biomolecule to keeping the nature ecologic equilibrium.Most microorganisms are present in the soil, and the microorganism of small part is present in atmospheric bottom with aerocolloidal form, and a part wherein has pathogenic, can bring threat to human beings'health when reaching finite concentration.Especially in recent years such as SARS, H5N1 bird flu, H1N1 influenza outburst in the whole world, bring great harm to human beings'health, bring massive losses to economic development, therefore, in time, easy, the air microbe in the monitoring of environmental is formed accurately, to ensureing that people ' s health has very important meaning.
Efficient, comprehensive sampling to air microbe is the prerequisite that air microbe accurately detects.The sample mode of traditional air microbe mainly contains following three kinds: based on the sampling method of solid medium, based on the sampling method of liquid sample medium and based on the sampling method of physical action and characteristic.Owing to be difficult to reflect in time, comprehensively and accurately the truth of air microbe composition based on the sampling method of solid medium, sampling method based on physics and characteristic is unfavorable to the survival of microorganism, the operation relative complex, therefore, the general sampling method that is based on the liquid sample medium of Shi Yonging.
Based on the sampling method of liquid sample medium, mainly contain cyclone method and ballistic method.The former utilizes the air flow at high speed, and promotion is collected liquid and formed whirlpool, the particulate matter in the portion of air is dissolved in collect in the liquid simultaneously, reaches the purpose of collecting air microbe.The latter be utilize jet-stream wind mode with airborne microorganism particle collection in the liquid of small size.Foremost have Porton sampling thief and an AGI-30 sampling thief.The advantage of these class methods is: the sampled form of sample is a fluid sample, sample subsequent treatment high flexible, and sample can dilute and concentrate, to obtain wider more accurate biometric sample information; Can be used for multiple detection technique: comprise agar plate cultivation, immunoassays, PCR (PCR), flow cytometer, microorganism particle counting and microscopic examination etc.; Simultaneously liquid has the certain protection effect to microorganism, can avoid microorganism in the process of collecting because the death that clashes and cause.But two kinds of sampling method ubiquity air and the problem fully that contacts inadequately of collecting liquid based on the liquid sample medium, thereby collection efficiency is not high.
[utility model content]
Based on this, be necessary the air microbe sampling system that provides a kind of microorganism-collecting efficient higher.
A kind of air microbe sampling system comprises: sampling bottle, draft tube, nozzle, escape pipe and air pump; Described draft tube stretches into and is communicated with described nozzle in the described sampling bottle and described nozzle fixedly is suspended in the described sampling bottle, and described escape pipe is communicated with described sampling bottle and air pump; The diversion division that described nozzle comprises main part and is located at described main part side and extends along described main part axis, wherein, described nozzle interior offers the air cavity that is communicated with described draft tube, the end that described nozzle is relative with described draft tube offers the impact opening that is communicated with described air cavity, and described diversion division is provided with the cyclone hole that is communicated with described air cavity.
In preferred embodiment, described diversion division is around the bending of the surface of described main part, and described diversion division comprises that the guide face that extends along overbending direction reaches and described guide face opposed inside face, and described cyclone hole is located on the described medial surface.
In preferred embodiment, described cyclone hole is the long and narrow perforate of extending along described columnar body axis direction, and described cyclone hole is arranged on the described medial surface side near described columnar body, and on described medial surface near an end of described impact opening.
In preferred embodiment, described impact opening comprises a plurality of long and narrow perforate that distributes around described main part axis.
In preferred embodiment, also comprise the oversampling capping adaptive with described sampling bottle, described oversampling capping is provided with the fixed orifice adaptive with described draft tube, and described draft tube is passed described fixed orifice and is fixed in the described oversampling capping.
In preferred embodiment, described escape pipe is located in the described oversampling capping.
In preferred embodiment, described draft tube, described escape pipe and described oversampling capping are formed in one.
In preferred embodiment, also comprise the draft tube gland bonnet adaptive, seal the preservation gland bonnet of preserving sample solution with the adaptive escape pipe gland bonnet of the endpiece of described escape pipe and with adaptive after sampling finishes, being used to of described sampling bottle with the inlet end of described draft tube.
In preferred embodiment, described draft tube comprises funnelform inlet portion and the interior atubular extension portion that is connected with described nozzle of described sampling bottle that stretches into that is communicated with described inlet portion.
In preferred embodiment, also comprise the flowmeter that is used to control or add up gas flow between described escape pipe and described air pump.
This air microbe sampling system adopts suction-type whirlpool water filtration principle, within a certain period of time the airborne microorganism of certain volume is carried out enrichment by the mode of liquid filtering, the bottom of nozzle is provided with impact opening, can utilize jet-stream wind that gas is formed more littler bubbles, offer the cyclone hole on the diversion division, can utilize the flow at high speed of air to promote to collect liquid formation whirlpool, promote the mutual extrusion between bubble, increase the contact area of air and pregnant solution, improve the collection efficiency of microorganism.
[description of drawings]
Fig. 1 is the diagrammatic cross-section of the air microbe sampling system of an embodiment;
Fig. 2 is the stereographic map of nozzle among Fig. 1;
Fig. 3 is the stereographic map at another visual angle of nozzle among Fig. 1;
Fig. 4 be among Fig. 2 nozzle along the cut-open view of IV-IV line;
Fig. 5 is a structural representation of gathering gland bonnet and escape pipe among Fig. 1.
[embodiment]
Mainly reaching specific embodiment below in conjunction with the accompanying drawings is described in further detail the air microbe sampling system.
As shown in Figure 1, the air microbe sampling system 100 of an embodiment comprises: sampling bottle 110, draft tube 120, nozzle 130, escape pipe 140, oversampling capping 150 and the air pump 160 adaptive with sampling bottle 110.Wherein, draft tube 120 stretches in the sampling bottle 110 and is communicated with nozzle 130.Nozzle 130 is fixedly connected on an end of draft tube 120 and is suspended in the sampling bottle 110.Escape pipe 140 is mainly used in and is communicated with sampling bottle 110 and air pump 160.
The sampling bottle 110 of present embodiment roughly is the flat bottom flask shape, comprises the neck part 112 of column and spherical bottle tripe portion 114.Bottle tripe portion 114 is mainly used in splendid attire sample solution (figure does not show).Preferably, nozzle 130 is suspended in bottle tripe portion 114, sample solution immersion nozzle 130.
Draft tube 120 comprises funnelform inlet portion 122 and the sampling bottle 110 interior atubular extension portions 124 that are connected with nozzle 130 that stretch into that are communicated with inlet portion 122.Inlet portion 122 is funnel-form, can increase the area that enters of gas, improves the bioaccumulation efficiency of air microbe.
To shown in Figure 4, nozzle 130 comprises main part 132 and is located at the side of main part 132 and the diversion division 134 that extends along main part 132 axis as Fig. 2.The nozzle 130 of present embodiment comprises 5 diversion divisions 134.5 diversion divisions 134 form the cross sectional shape of impeller shape around the bending of the surface of main part 132 and with main part 132, as shown in Figure 4.In other embodiment, diversion division 134 can also be 1,2,3,4,7,8 or more.A plurality of diversion divisions 134 can evenly distribute on the surface of main part 132.The inside of nozzle 130 offers the air cavity 322 that is communicated with draft tube 120, also is that whole nozzle 130 is the shell structure of boring.
Nozzle 130 connects a relative end with draft tube 120 and offers the impact opening 324 that is communicated with air cavity 322.Impact opening 324 can be a plurality of point-like apertures of offering at nozzle 130 1 ends, and the also a plurality of long and narrow perforate that can distribute for the axis around main part 132 is as a plurality of impact openings 324 in the present embodiment.Impact opening 324 is mainly used in and produces the air-flow that impacts downwards, when running into sample solution the gas of collecting is broken into more less bubble, increases the contact area of gas and liquid, improves the bioaccumulation efficiency of microorganism.
Diversion division 134 comprises guide face 342 and and the guide face 342 opposed inside faces 344 that extend along overbending direction.Offer the cyclone hole 346 that is used to drive the sample solution rotation on the medial surface 344.The air-flow of cyclone hole 346 ejections drives sample solution and rotatablely moves in the same way around the motion of diversion division 134 overbending directions.
As shown in Figure 5, the escape pipe 140 of present embodiment is located in the oversampling capping 150, and is one-body molded with oversampling capping 150, and ease of assembly, dismounting reach preserves the sealing of sampling bottle 110.In other embodiments, escape pipe 140 also can directly perforate formation on the neck part 112 of column.
Offer the fixed orifice 152 adaptive in the oversampling capping 150 with draft tube 120.Draft tube 120 is passed fixed orifice 152 and is fixed in the oversampling capping 150.In other embodiments, oversampling capping 150 can also be one-body molded with sampling bottle 110, and better tightness has been omitted gland bonnet 150 this moment.
Preferably, draft tube 120, escape pipe 140 and oversampling capping 150 are formed in one, thus in the air microbe sampling process ease of assembly, after sampling finishes, be convenient to dismounting, reduce the running time, can avoid sample solution to pollute.
Accordingly, this air microbe sampling system 100 comprises that also the draft tube gland bonnet adaptive with the inlet end of draft tube 120 (figure does not show), the escape pipe gland bonnet (figure do not show) adaptive with the endpiece of escape pipe 140 reach and sampling bottle 110 adaptive being used to seal the preservation gland bonnet (figure does not show) of preserving sample solution after sampling finishes.
Air microbe sampling system 100 each parts assembling place of present embodiment all adopt the screw thread rotation mode to be connected and sealed, as between draft tube 120 and the oversampling capping 150, oversampling capping 150 with between the sampling bottle 110, draft tube 120 is with between the nozzle 130, between draft tube gland bonnet and the draft tube 120, between escape pipe gland bonnet and the escape pipe 140 and preserve between gland bonnet and the sampling bottle 110 etc., all employing is threaded.In other embodiments, can also adopt buckle structure, corresponding buckle part is provided with seals such as sealing gasket.
In addition, further preferred, be statistics and pilot-gas flow, this air microbe sampling system 100 also comprises the flowmeter (figure does not show) that is arranged between escape pipe 140 and the air pump 160.Air microbe sampling system 100 use traffic meter as required is provided with collecting flow and time parameter etc.
Use 100 pairs of air microbes of above-mentioned air microbe sampling system and collect, generally comprise following steps:
Step S1. sterilization:
Placing sterilizing bag to do autoclaving together with sampling bottle 110, draft tube gland bonnet, escape pipe gland bonnet and preservation gland bonnet draft tube 120, nozzle 130, escape pipe 140 and oversampling capping 150 handles.
Step S2. assembling:
After sterilization is finished, under aseptic condition, at first draft tube 120, nozzle 130, escape pipe 140 and oversampling capping 150 are assembled; The oversampling capping 150 that will be connected with draft tube 120, nozzle 130 and escape pipe 140 is then assembled with the sampling bottle 110 that sample solution is housed; Seal draft tube 120 and the corresponding draft tube gland bonnet of escape pipe 130 usefulness and escape pipe gland bonnet standby at last.
Step S3. collects:
In collection location, remove draft tube gland bonnet, escape pipe gland bonnet after, external equipments such as the same gas meter of escape pipe, air pump connected finish, the beginning collection process is set collecting flow and time parameter etc. as required.
Step S4. sealing is preserved and subsequent detection:
After collection finishes, take off the oversampling capping 150 that is connected with draft tube 120, nozzle 130 and escape pipe 140, the capping of the corresponding preservation of sampling bottle 110 usefulness gland bonnet is good, sample solution in the sampling bottle 110 is used for follow-up microorganism detection, as, can carry out nucleic acid extraction and (at first sample solution be carried out centrifugal treating, in precipitation, add lysate then, adopt phenol-chloroform extraction process or glass bead method to carry out extraction, the purifying of nucleic acid), the method for perhaps utilizing plate count is added up etc. cultivating microorganism in the air.
This air microbe sampling system 100 adopts suction-type whirlpool water filtration principle, within a certain period of time the airborne microorganism of certain volume is carried out enrichment by the mode of liquid filtering, the bottom of nozzle 130 is provided with impact opening 324, can utilize jet-stream wind that gas is formed more littler bubbles, offer cyclone hole 346 on the diversion division 134, can utilize the flow at high speed of air to promote to collect liquid formation whirlpool, promote the mutual extrusion between bubble, increase the contact area of air and pregnant solution, improve the collection efficiency of microorganism.
The above embodiment has only expressed several embodiment of the present utility model, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to the utility model claim.Should be pointed out that for the person of ordinary skill of the art under the prerequisite that does not break away from the utility model design, can also make some distortion and improvement, these all belong to protection domain of the present utility model.Therefore, the protection domain of the utility model patent should be as the criterion with claims.
Claims (10)
1. an air microbe sampling system is characterized in that, comprising: sampling bottle, draft tube, nozzle, escape pipe and air pump; Described draft tube stretches into and is communicated with described nozzle in the described sampling bottle and described nozzle fixedly is suspended in the described sampling bottle, and described escape pipe is communicated with described sampling bottle and air pump; The diversion division that described nozzle comprises main part and is located at described main part side and extends along described main part axis, wherein, described nozzle interior offers the air cavity that is communicated with described draft tube, the end that described nozzle is relative with described draft tube offers the impact opening that is communicated with described air cavity, and described diversion division is provided with the cyclone hole that is communicated with described air cavity.
2. air microbe sampling as claimed in claim 1 system, it is characterized in that, described diversion division is around the bending of the surface of described main part, and described diversion division comprises that the guide face that extends along overbending direction reaches and described guide face opposed inside face, and described cyclone hole is located on the described medial surface.
3. air microbe sampling as claimed in claim 1 or 2 system, it is characterized in that, described cyclone hole is the long and narrow perforate of extending along described columnar body axis direction, described cyclone hole is arranged on the described medial surface side near described columnar body, and on described medial surface near an end of described impact opening.
4. air microbe sampling as claimed in claim 1 system is characterized in that, described impact opening comprises a plurality of long and narrow perforate that distributes around described main part axis.
5. air microbe sampling as claimed in claim 1 system, it is characterized in that, also comprise the oversampling capping adaptive with described sampling bottle, described oversampling capping is provided with the fixed orifice adaptive with described draft tube, and described draft tube is passed described fixed orifice and is fixed in the described oversampling capping.
6. air microbe sampling as claimed in claim 5 system is characterized in that described escape pipe is located in the described oversampling capping.
7. air microbe sampling as claimed in claim 5 system is characterized in that described draft tube, described escape pipe and described oversampling capping are formed in one.
8. air microbe sampling as claimed in claim 1 system, it is characterized in that, also comprise the draft tube gland bonnet adaptive, seal the preservation gland bonnet of preserving sample solution with the adaptive escape pipe gland bonnet of the endpiece of described escape pipe and with adaptive after sampling finishes, being used to of described sampling bottle with the inlet end of described draft tube.
9. air microbe sampling as claimed in claim 1 system is characterized in that, described draft tube comprises funnelform inlet portion and the interior atubular extension portion that is connected with described nozzle of described sampling bottle that stretches into that is communicated with described inlet portion.
10. air microbe sampling as claimed in claim 1 system is characterized in that, also comprises the flowmeter that is used to control or add up gas flow between described escape pipe and described air pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201020678465XU CN202002827U (en) | 2010-12-24 | 2010-12-24 | Air microbial sampling system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201020678465XU CN202002827U (en) | 2010-12-24 | 2010-12-24 | Air microbial sampling system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202002827U true CN202002827U (en) | 2011-10-05 |
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ID=44705564
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201020678465XU Expired - Fee Related CN202002827U (en) | 2010-12-24 | 2010-12-24 | Air microbial sampling system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202002827U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105056648A (en) * | 2015-08-17 | 2015-11-18 | 麦克传感器股份有限公司 | Dustproof filtering nozzle |
| CN109100184A (en) * | 2018-07-26 | 2018-12-28 | 杜瑞青 | It is a kind of to pre-process sampling collector for the underground mine gas containing a large amount of dusts |
-
2010
- 2010-12-24 CN CN201020678465XU patent/CN202002827U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105056648A (en) * | 2015-08-17 | 2015-11-18 | 麦克传感器股份有限公司 | Dustproof filtering nozzle |
| CN109100184A (en) * | 2018-07-26 | 2018-12-28 | 杜瑞青 | It is a kind of to pre-process sampling collector for the underground mine gas containing a large amount of dusts |
| CN109100184B (en) * | 2018-07-26 | 2020-11-20 | 嘉兴嘉卫检测科技有限公司 | Underground mine gas pretreatment sampling collector containing a large amount of dust |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111005 Termination date: 20111224 |