CN102154095B - Air microorganism sampling device - Google Patents
Air microorganism sampling device Download PDFInfo
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- CN102154095B CN102154095B CN 201010604110 CN201010604110A CN102154095B CN 102154095 B CN102154095 B CN 102154095B CN 201010604110 CN201010604110 CN 201010604110 CN 201010604110 A CN201010604110 A CN 201010604110A CN 102154095 B CN102154095 B CN 102154095B
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Abstract
The invention relates to an air microorganism sampling device. The air microorganism sampling device adopts a suction circinate water filtering principle. In a certain time, microorganisms in certain volume of air are enriched by a liquid filtering mode. The bottom end of a spray nozzle is provided with an impact hole. Air can be formed into more and smaller air bubbles by jet streams. A flow guide part is provided with a cyclonic hole. Drips can be pushed to form whirlpools by high-speed flowing of the air. Manual extrusion of the air bubbles is promoted. The contact surface of the air and enrichment liquid is increased. The collecting efficiency of the microorganisms is improved.
Description
[technical field]
The present invention relates to a kind of microbiological sampler, particularly a kind of air microorganism sampling device.
[background technology]
Microorganism plays very important effect as the important biomolecule that participates in material cycle to keeping the nature eubiosis.Most microorganisms are present in soil, and the microorganism of small part is present in atmospheric bottom with aerocolloidal form, and a part wherein has pathogenic, bring threat can for when reaching finite concentration the mankind's health.Especially in recent years such as SARS, H5N1 bird flu, the H1N1 influenza outburst in the whole world, brought great harm for the mankind's health, bring massive losses to Economic development, therefore, in time, easy, the air microbe in monitoring of environmental forms accurately, to ensureing that people ' s health has very important meaning.
Efficient, comprehensive sampling to air microbe is the prerequisite that air microbe accurately detects.The sample mode of traditional air microbe mainly contains following three kinds: based on the sampling method of solid medium, based on the sampling method of liquid sample medium and based on the sampling method of physical action and characteristic.The truth that is difficult to reflect in time, comprehensively and accurately the air microbe composition due to the sampling method based on solid medium, sampling method based on physics and characteristic is unfavorable to the survival of microorganism, the operation relative complex, therefore, the sampling method that is based on the liquid sample medium that generally uses.
Based on the sampling method of liquid sample medium, mainly contain cyclone method and ballistic method.The former utilizes the air flow at high speed, and promotion is collected liquid and formed whirlpool, the particulate matter in portion of air is dissolved in collect in liquid simultaneously, reaches the purpose of collecting air microbe.The latter be utilize jet-stream wind mode with airborne microorganism particle collection in the liquid of small volume.Foremost have Porton sampling thief and an AGI-30 sampling thief.The advantage of these class methods is: the sampled form of sample is liquid sample, sample subsequent disposal high flexible, and sample can dilute and concentrate, to obtain wider more accurate biometric sample information; Can be used for multiple detection technique: comprise agar plate culture, immunoassay, PCR (polymerase chain reaction), flow cytometer, microorganism particle counting and microscopic examination etc.; Liquid has certain provide protection to microorganism simultaneously, the death that can avoid microorganism to cause owing to clashing in the process of collecting.But two kinds of sampling method ubiquity air and the problem not fully that contacts of collecting liquid based on the liquid sample medium, thereby collection effciency is not high.
[summary of the invention]
Based on this, be necessary to provide a kind of microorganism-collecting efficient higher air microorganism sampling device.
A kind of air microorganism sampling device comprises: sampling bottle, inlet pipe, nozzle, and escape pipe; Described inlet pipe stretches into and is communicated with described nozzle in described sampling bottle and described nozzle fixedly is suspended in described sampling bottle; Described nozzle comprises main part and is located at described main part side and along the diversion division of described main part Axis Extension, wherein, described nozzle interior offers the air cavity that is communicated with described inlet pipe, the end that described nozzle is relative with described inlet pipe offers the impact opening that is communicated with described air cavity, and described diversion division is provided with the cyclone hole that is communicated with described air cavity.
In preferred embodiment, described diversion division is around the bending of the surface of described main part, and described diversion division comprises guide face and the medial surface relative with described guide face that extends along overbending direction, and described cyclone hole is located on described medial surface.
In preferred embodiment, described cyclone hole is the long and narrow perforate of extending along described main part axis direction, described cyclone hole on described medial surface near a side setting of described main part.
In preferred embodiment, an end setting of described cyclone hole close described impact opening on described medial surface.
In preferred embodiment, described impact opening comprises a plurality of long and narrow perforate around the axis distribution of described main part.
In preferred embodiment, also comprise the oversampling capping adaptive with described sampling bottle, described oversampling capping is provided with the fixed orifices adaptive with described inlet pipe, and described inlet pipe is passed described fixed orifices and is fixed in described oversampling capping.
In preferred embodiment, described escape pipe is located in described oversampling capping.
In preferred embodiment, described inlet pipe, described escape pipe and described oversampling capping are formed in one.
In preferred embodiment, also comprise the inlet pipe sealing cover adaptive with the inlet end of described inlet pipe, with the adaptive escape pipe sealing cover of the exit end of described escape pipe and with described sampling bottle adaptive be used for the preservation sealing cover that sample solution is preserved in sealing after sampling is complete.
In preferred embodiment, described inlet pipe comprises funnelform inlet portion and the described sampling bottle interior atubular extension section that is connected with described nozzle of stretching into that is communicated with described inlet portion.
This air microorganism sampling device adopts inhaling type whirlpool water filtration principle, within a certain period of time the airborne microorganism of certain volume is carried out enrichment by the mode of liquid filtering, the bottom of nozzle is provided with impact opening, can utilize jet-stream wind that gas is formed more less bubbles, offer the cyclone hole on diversion division, can utilize the flow at high speed of air to promote to collect liquid formation whirlpool, promote the mutual extruding between bubble, increase the contact area of air and pregnant solution, improve the collection effciency of microorganism.
[description of drawings]
Fig. 1 is the diagrammatic cross-section of the air microorganism sampling device of an embodiment;
Fig. 2 is the stereographic map of nozzle in Fig. 1;
Fig. 3 is the stereographic map at another visual angle of nozzle in Fig. 1;
Fig. 4 be in Fig. 2 nozzle along the sectional view of IV-IV line;
Fig. 5 gathers the structural representation of sealing cover and escape pipe in Fig. 1.
[embodiment]
The below mainly is described in further detail air microorganism sampling device in conjunction with the drawings and the specific embodiments.
As shown in Figure 1, the air microorganism sampling device 100 of an embodiment comprises: sampling bottle 110, inlet pipe 120, nozzle 130, escape pipe 140 and the oversampling capping 150 adaptive with sampling bottle 110.Wherein, inlet pipe 120 stretches in sampling bottle 110 and is communicated with nozzle 130.Nozzle 130 is fixedly connected on an end of inlet pipe 120 and is suspended in sampling bottle 110.Escape pipe 140 is mainly used in being communicated with sampling bottle 110 and external equipment (as under meter, vacuum fan etc.).
The sampling bottle 110 of the present embodiment roughly is the Florence flask shape, comprises neck part 112 and the spherical bottle tripe section 114 of column.Bottle tripe section 114 is mainly used in splendid attire sample solution (not shown).Preferably, nozzle 130 is suspended in bottle tripe section 114, sample solution immersion nozzle 130.
Inlet pipe 120 comprises funnelform inlet portion 122 and the sampling bottle 110 interior atubular extension section that is connected with nozzle 130 124 of stretching into that is communicated with inlet portion 122.Inlet portion 122 is funnel-form, can increase the area that enters of gas, improves the bioaccumulation efficiency of air microbe.
To shown in Figure 4, nozzle 130 comprises main part 132 and is located at the side of main part 132 and along the diversion division 134 of main part 132 Axis Extensions as Fig. 2.The nozzle 130 of the present embodiment comprises 5 diversion divisions 134.5 diversion divisions 134 bend and form the cross-sectional shape of impeller shape together with main part 132 around the surface of main part 132, as shown in Figure 4.In other embodiment, diversion division 134 can also be 1,2,3,4,7,8 or more.A plurality of diversion divisions 134 can distribute at the surface uniform of main part 132.The inside of nozzle 130 offers the air cavity 322 that is communicated with inlet pipe 120, is also that whole nozzle 130 is the shell structure of boring.
Nozzle 130 connects a relative end with inlet pipe 120 and offers the impact opening 324 that is communicated with air cavity 322.Impact opening 324 can be a plurality of point-like apertures of offering at nozzle 130 1 ends, and a plurality of long and narrow perforate that also can distribute for the axis around main part 132 is as a plurality of impact openings 324 in the present embodiment.Impact opening 324 is broken into more less bubble with the gas of collecting mainly for generation of the air-flow that impacts when running into sample solution downwards, increases the contact area of gas and liquid, improves the bioaccumulation efficiency of microorganism.
As shown in Figure 5, the escape pipe 140 of the present embodiment is located in oversampling capping 150, and is one-body molded with oversampling capping 150, and ease of assembly, dismounting reach preserves the sealing of sampling bottle 110.In other embodiments, escape pipe 140 also can directly perforate formation on the neck part 112 of column.
Offer the fixed orifices 152 adaptive with inlet pipe 120 in oversampling capping 150.Inlet pipe 120 is passed fixed orifices 152 and is fixed in oversampling capping 150.In other embodiments, oversampling capping 150 can also be one-body molded with sampling bottle 110, and better tightness has been omitted sealing cover 150 this moment.
Preferably, inlet pipe 120, escape pipe 140 and oversampling capping 150 are formed in one, thus in the air microbe sampling process ease of assembly, after sampling is complete, be convenient to dismounting, reduce the operating time, can avoid sample solution to pollute.
Accordingly, this air microorganism sampling device 100 also comprise the inlet pipe sealing cover (not shown) adaptive with the inlet end of inlet pipe 120, with the adaptive escape pipe sealing cover (not shown) of the exit end of escape pipe 140 and with sampling bottle 110 adaptive be used for the preservation sealing cover (not shown) that sample solution is preserved in sealing after sampling is complete.
Air microorganism sampling device 100 each parts assemblings place of the present embodiment all adopt the screw thread rotation mode to be connected and sealed, as between inlet pipe 120 and oversampling capping 150, oversampling capping 150 with between sampling bottle 110, inlet pipe 120 is with between nozzle 130, between inlet pipe sealing cover and inlet pipe 120, between escape pipe sealing cover and escape pipe 140 and preserve between sealing cover and sampling bottle 110 etc., all employing is threaded.In other embodiments, can also adopt buckle structure, corresponding buckle part is provided with the sealing members such as gasket.
Use 100 pairs of air microbes of above-mentioned air microorganism sampling device and collect, generally comprise following steps:
Step S1. sterilization:
Inlet pipe 120, nozzle 130, escape pipe 140 and oversampling capping 150 are placed in sterilizing bag together with sampling bottle 110, inlet pipe sealing cover, escape pipe sealing cover and preservation sealing cover do the autoclaving processing.
Step S2. assembling:
After sterilization is completed, at first inlet pipe 120, nozzle 130, escape pipe 140 and oversampling capping 150 are assembled under aseptic condition; The oversampling capping 150 that then will be connected with inlet pipe 120, nozzle 130 and escape pipe 140 is assembled with the sampling bottle 110 that sample solution is housed; Seal inlet pipe 120 and the escape pipe 130 corresponding inlet pipe sealing covers of use and escape pipe sealing cover standby at last.
Step S3. collects:
In collection location, after removing inlet pipe sealing cover, escape pipe sealing cover, that the connection of the external equipments such as the same gas meter of escape pipe, vacuum fan is complete, the beginning collection process is set collecting flow and time parameter etc. as required.
Step S4. sealing is preserved and subsequent detection:
Collect complete after, take off the oversampling capping 150 that is connected with inlet pipe 120, nozzle 130 and escape pipe 140, the capping of the corresponding preservation of sampling bottle 110 use sealing cover is good, sample solution in sampling bottle 110 is used for follow-up microorganism detection, as, can carry out nucleic acid extraction and (at first sample solution be carried out centrifugal treating, then add lysate in precipitation, adopt phenol-chloroform extraction process or glass bead method to carry out extraction, the purifying of nucleic acid), but perhaps utilize the method for plate count culturing micro-organisms in air to be added up etc.
This air microorganism sampling device 100 adopts inhaling type whirlpool water filtration principle, within a certain period of time the airborne microorganism of certain volume is carried out enrichment by the mode of liquid filtering, the bottom of nozzle 130 is provided with impact opening 324, can utilize jet-stream wind that gas is formed more less bubbles, offer cyclone hole 346 on diversion division 134, can utilize the flow at high speed of air to promote to collect liquid formation whirlpool, promote the mutual extruding between bubble, increase the contact area of air and pregnant solution, improve the collection effciency of microorganism.
The above embodiment has only expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to the scope of the claims of the present invention.Should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (6)
1. an air microorganism sampling device, is characterized in that, comprising: sampling bottle, inlet pipe, nozzle, and escape pipe; Described inlet pipe stretches into and is communicated with described nozzle in described sampling bottle and described nozzle fixedly is suspended in described sampling bottle; Described nozzle comprises main part and is located at described main part side and along the diversion division of described main part Axis Extension, wherein, described nozzle interior offers the air cavity that is communicated with described inlet pipe, the end that described nozzle is relative with described inlet pipe offers the impact opening that is communicated with described air cavity, and described diversion division is provided with the cyclone hole that is communicated with described air cavity; Described diversion division is around the bending of the surface of described main part, and described diversion division comprises guide face and the medial surface relative with described guide face that extends along overbending direction, and described cyclone hole is located on described medial surface; Described cyclone hole is the long and narrow perforate of extending along described main part axis direction, a side setting of described cyclone hole close described main part on described medial surface; One end setting of described cyclone hole close described impact opening on described medial surface; Described impact opening comprises a plurality of long and narrow perforate around the axis distribution of described main part.
2. air microorganism sampling device as claimed in claim 1, it is characterized in that, also comprise the oversampling capping adaptive with described sampling bottle, described oversampling capping is provided with the fixed orifices adaptive with described inlet pipe, and described inlet pipe is passed described fixed orifices and is fixed in described oversampling capping.
3. air microorganism sampling device as claimed in claim 2, is characterized in that, described escape pipe is located in described oversampling capping.
4. air microorganism sampling device as claimed in claim 2, is characterized in that, described inlet pipe, described escape pipe and described oversampling capping are formed in one.
5. air microorganism sampling device as claimed in claim 1, it is characterized in that, also comprise the inlet pipe sealing cover adaptive with the inlet end of described inlet pipe, with the adaptive escape pipe sealing cover of the exit end of described escape pipe and with described sampling bottle adaptive be used for the preservation sealing cover that sample solution is preserved in sealing after sampling is complete.
6. air microorganism sampling device as claimed in claim 1, is characterized in that, described inlet pipe comprises funnelform inlet portion and the described sampling bottle interior atubular extension section that is connected with described nozzle of stretching into that is communicated with described inlet portion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010604110 CN102154095B (en) | 2010-12-24 | 2010-12-24 | Air microorganism sampling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010604110 CN102154095B (en) | 2010-12-24 | 2010-12-24 | Air microorganism sampling device |
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| CN102154095A CN102154095A (en) | 2011-08-17 |
| CN102154095B true CN102154095B (en) | 2013-06-19 |
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| CN 201010604110 Expired - Fee Related CN102154095B (en) | 2010-12-24 | 2010-12-24 | Air microorganism sampling device |
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Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102634449B (en) * | 2012-03-30 | 2013-11-06 | 中国检验检疫科学研究院 | Viral aerosol collecting and enriching instrument |
| CN103756894B (en) * | 2014-01-06 | 2015-04-08 | 中国科学院合肥物质科学研究院 | Liquid impact-type air microorganism sampler |
| CN105610061A (en) * | 2016-02-28 | 2016-05-25 | 南京开关厂有限公司 | High-low voltage switch cabinet |
| CN105811272B (en) * | 2016-04-15 | 2018-01-12 | 上海柘中电气有限公司 | High-low voltage switch cabinet with excellent heat dispersion performance |
| CN105811273B (en) * | 2016-04-15 | 2018-03-09 | 宁波新胜中压电器有限公司 | A kind of moistureproof good High-low voltage switch cabinet that radiates |
| US10732081B2 (en) * | 2016-08-15 | 2020-08-04 | Veltek Associates, Inc. | Portable air sampler |
| CN106085830B (en) * | 2016-08-30 | 2019-03-19 | 浙江省疾病预防控制中心 | Pathogenic microorganism collection device in air |
| CN110408523A (en) * | 2019-07-30 | 2019-11-05 | 中国地质大学（武汉） | A kind of baffle arrangement improving impacting type air microorganism sampler sampling efficiency |
| CN111781015B (en) * | 2020-07-30 | 2023-07-21 | 中国检验检疫科学研究院 | Optimization method and device for sampling efficiency of gas-liquid mixing biological aerosol collecting device |
| CN114058498B (en) * | 2022-01-11 | 2022-04-19 | 至美时代生物智能科技(北京)有限公司 | Full-automatic air microorganism detection system and detection method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5766958A (en) * | 1995-09-12 | 1998-06-16 | Sullivan; George D. | Method for detecting and collecting infectious airborne microorganisms for rapid identification |
| CN101392221A (en) * | 2008-11-03 | 2009-03-25 | 中国人民解放军军事医学科学院微生物流行病研究所 | Mass flow air microorganism sampling device |
| CN101974418A (en) * | 2010-10-24 | 2011-02-16 | 青岛众瑞智能仪器有限公司 | Miniature personal microbial air sampler |
| CN102323115A (en) * | 2011-08-27 | 2012-01-18 | 福州大学 | Air sampler |
-
2010
- 2010-12-24 CN CN 201010604110 patent/CN102154095B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5766958A (en) * | 1995-09-12 | 1998-06-16 | Sullivan; George D. | Method for detecting and collecting infectious airborne microorganisms for rapid identification |
| CN101392221A (en) * | 2008-11-03 | 2009-03-25 | 中国人民解放军军事医学科学院微生物流行病研究所 | Mass flow air microorganism sampling device |
| CN101974418A (en) * | 2010-10-24 | 2011-02-16 | 青岛众瑞智能仪器有限公司 | Miniature personal microbial air sampler |
| CN102323115A (en) * | 2011-08-27 | 2012-01-18 | 福州大学 | Air sampler |
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