CN201817467U

CN201817467U - Laser side exciting real-time fluorescent PCR (Photo-conductive Relay) amplifier

Info

Publication number: CN201817467U
Application number: CN201020272810XU
Authority: CN
Inventors: 崔碧峰; 杜晓东; 郭伟玲
Original assignee: Beijing University of Technology
Current assignee: Beijing University of Technology
Priority date: 2010-07-23
Filing date: 2010-07-23
Publication date: 2011-05-04
Anticipated expiration: 2020-07-23

Abstract

The utility model discloses a laser side exciting real-time fluorescent PCR (Photo-conductive Relay) amplifier, belonging to the field of intersection photoelectric detection technology and biotechnology. The laser side exciting real-time fluorescent PCR (Photo-conductive Relay) amplifier comprises a PCR amplifier base, a heating table, a light source module supporting rod, a sample cell module and a laser light source module, wherein the heating table is fixed on the PCR amplifier base, the laser light source module is fixed on the PCR amplifier base through the light source module supporting rod, and the sample cell module is arranged on the heating table. The sample cell module is formed by combining a micro test tube, a Cato test tube and a heat-conducting base, so that the sample collecting quantity, the fluorescent dosage quantity and detection time can be reduced, the cost can be lowered, the detection efficiency can be improved, and the use is convenient. By adopting the laser light source side exciting, the laser side exciting real-time fluorescent PCR amplifier can omit the complex optical system, is simplified in structure, improves the stability of the system, prolongs the service life of the light source, and reduces the production cost.

Description

The laser side excites the real-time fluorescence PCR instrument

Technical field

The utility model discloses a kind of laser side and excite the structure of real-time fluorescence PCR instrument, belong to detecting technique and biotechnology crossing domain.

Background technology

At present the excitation light source that adopts of PCR instrument is tungsten halogen lamp or xenon lamp substantially, and these light sources are white light sources, and spectrum has almost covered whole visible light, and PCR instrument labelled reagent is selectively to the wavelength of excitation light source.Adopt the main drawback of these light sources to be: 1. to contain not environmental protection of heavy metal substance; 2. high energy consumption; 3. short life; 4. stray light is too many, interference detection.

At present in order to get rid of the interference of stray light, adopt the method that excitation light source and labelled reagent radiant light are carried out filtering to surveying.The shortcoming of this method is: the 1. intensity of filter plate decay excitation light source; 2. the radiant light intensity of filter plate decay labelled reagent; 3. increased the cost of product; 4. increased the product difficulty of processing.

Traditional PCR instrument, excitation light source and detector are positioned at the sample pool both sides, and exciting light shines from the sample pool upper surface, the labelled reagent radiant light is simultaneously from the upper surface outgoing.This structure need be introduced complicated optical system and come separately exciting light and radiant light, and difficulty of processing is big, the cost height.

Summary of the invention

The purpose of this utility model will overcome above-mentioned the deficiencies in the prior art exactly, provide a kind of simple in structure, be easy to assembling, energy-conserving and environment-protective, and the laser side that can reduce cost significantly excites the real-time fluorescence PCR instrument.

Above-mentioned purpose of the present utility model is achieved through the following technical solutions: the laser side excites the real-time fluorescence PCR instrument, comprise PCR instrument base 1, warm table 2, light source module pole 3, sample pool module 4, laser light source module 5, warm table 2 is fixed on the PCR instrument base 1, laser light source module 5 is fixed on the PCR instrument base 1 by light source module pole 3, and sample pool module 4 is placed on the warm table 2 and is positioned among the laser light source module 5.

Described modular member sample pool module 4 comprises heat-conducting base 4-1, little test tube Kato 4-2, little test tube 4-3; Little test tube Kato 4-2 links together a plurality of little test tube 4-3 by the circular hole on it, and its integral body is placed on the heat-conducting base 4-1, makes little test tube 4-3 bottom corresponding one by one with groove on the heat-conducting base 4-1, and the heat-conducting silicone grease of fitting between the two.

Described little test tube heat-conducting base 4-1 adopts gold-plated oxygen free copper or gold-plated tungsten-copper alloy, and the groove on it is the curved surface with little test tube bottom surface same curvature.

Described little test tube Kato 4-2 adopts flexible heat resistant plastice.

Described laser light source module 5 is a modular member, comprises light source framework 5-1, wiring board 5-2, semiconductor laser 5-3; Semiconductor laser 5-3 is integrated on the wiring board 5-2, and wiring board is assembled on the light source framework by draw-in groove.

Described semiconductor laser 5-3 light hole place is packaged with extender lens.

Compare with existing P CR instrument, the utility model can obtain following beneficial effect:

Use semiconductor laser as excitation light source, be equipped with rational design, have advantages such as stable, energy-conservation, narrow spectrum.

The sample pool side excites, and the upper surface detecting structure can save complicated optical system, is convenient to processing, can reduce manufacturing cost significantly.

The utility model compactness rational in infrastructure is manufactured easily, and it is convenient to assemble, and energy-conserving and environment-protective can cut down finished cost long service life significantly.

Description of drawings

Fig. 1 is a positive perspective view of the present utility model;

Fig. 2 is a decomposition texture synoptic diagram of the present utility model;

Fig. 3 is sample pool module 4 positive perspective view of the present utility model;

Fig. 4 is sample pool module 4 decomposition texture synoptic diagram of the present utility model;

Fig. 5 is laser light source module 5 decomposition texture synoptic diagram of the present utility model;

Fig. 6 is the cross section structure synoptic diagram of sample pool module 4;

Fig. 7 is a cross section structure synoptic diagram of the present utility model;

Among the figure: 1, PCR instrument base, 2, warm table, 3, light source module pole, 4, the sample pool module, 4-1, heat-conducting base, 4-2, Kato, 4-3, little test tube, 5, laser light source module, 5-1, light source module framework, 5-2, wiring board, 5-3, semiconductor laser.

Embodiment

The utility model is further described below in conjunction with the drawings and specific embodiments.

As shown in Figure 1 and Figure 2, the laser side excites the real-time fluorescence PCR instrument, comprises PCR instrument base 1, warm table 2, light source module pole 3, sample pool module 4, laser light source module 5; Warm table 2 is fixed on the PCR instrument base 1, and laser light source module 5 is fixed on the PCR instrument base 1 by light source module pole 3, and sample pool module 4 is placed on the warm table 2 and is positioned among the laser light source module 5.

As shown in Figure 3, Figure 4, sample pool module 4 is a modular member, comprises heat-conducting base 4-1, little test tube Kato 4-2, little test tube 4-3; Little test tube Kato 4-2 links together a plurality of little test tube 4-3 by the circular hole on it, its integral body is placed on the heat-conducting base 4-1, and makes little test tube 4-3 bottom corresponding one by one with groove on the heat-conducting base 4-1.

As shown in Figure 5, laser light source module 5 is a modular member, comprises light source framework 5-1, wiring board 5-2, semiconductor laser 5-3; Semiconductor laser 5-3 is integrated on the wiring board 5-2, and wiring board is assembled on the light source framework by draw-in groove.

As shown in Figure 2, four groups of sample pool modules of this exemplary application, every group of sample pool be by a heat-conducting base 4-1, a little test tube Kato 4-2,2 * 8 little test tube 4-3 form.The heat-conducting silicone grease of fitting between little test tube 4-3 bottom and the heat-conducting base 4-1 groove.Equally, laser light source module 5 comprises a light source framework 5-1,8 wiring boards, 64 semiconductor lasers.

Claims

1. the laser side excites the real-time fluorescence PCR instrument, it is characterized in that: comprise PCR instrument base (1), warm table (2), light source module pole (3), sample pool module (4), laser light source module (5); Wherein: warm table (2) is fixed on the PCR instrument base (1), and laser light source module (5) is fixed on the PCR instrument base (1) by light source module pole (3), and sample pool module (4) is placed on the warm table (2) and is positioned among the laser light source module (5).

2. laser according to claim 1 side excites the real-time fluorescence PCR instrument, it is characterized in that: sample pool module (4) is a modular member, comprises heat-conducting base (4-1), little test tube Kato (4-2), little test tube (4-3); Little test tube Kato (4-2) links together a plurality of little test tubes (4-3) by the circular hole on it, its integral body is placed on the heat-conducting base (4-1), and make little test tube (4-3) bottom corresponding one by one with groove on the heat-conducting base (4-1), the heat-conducting silicone grease of fitting between the two.

3. laser according to claim 2 side excites the real-time fluorescence PCR instrument, it is characterized in that: described heat-conducting base (4-1) adopts gold-plated oxygen free copper or gold-plated tungsten-copper alloy, and the groove on it is the curved surface with little test tube bottom surface same curvature.

4. laser according to claim 2 side excites the real-time fluorescence PCR instrument, it is characterized in that: described little test tube Kato (4-2) adopts flexible heat resistant plastice.

5. laser according to claim 1 side excites the real-time fluorescence PCR instrument, it is characterized in that: laser light source module (5) is a modular member, comprises light source framework (5-1), wiring board (5-2), semiconductor laser (5-3); Semiconductor laser (5-3) is integrated on the wiring board (5-2), and wiring board is assembled on the light source framework (5-1) by draw-in groove.

6. laser according to claim 5 side excites the real-time fluorescence PCR instrument, it is characterized in that: described semiconductor laser (5-3) light hole place is packaged with extender lens.