CN201607421U - Planar biological detection test piece - Google Patents

Planar biological detection test piece Download PDF

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Publication number
CN201607421U
CN201607421U CN2010201292536U CN201020129253U CN201607421U CN 201607421 U CN201607421 U CN 201607421U CN 2010201292536 U CN2010201292536 U CN 2010201292536U CN 201020129253 U CN201020129253 U CN 201020129253U CN 201607421 U CN201607421 U CN 201607421U
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CN
China
Prior art keywords
test piece
slide glass
film
electrode end
detecting
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Expired - Fee Related
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CN2010201292536U
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Chinese (zh)
Inventor
段纬
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Hivox Biotek Inc
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Hivox Biotek Inc
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Abstract

The utility model provides a planar biological detection test piece, which comprises a slide and an enzyme reaction zone. A conducting circuit formed by means of evaporation is arranged on the slide, the front end and the rear end of the conducting circuit are sequentially provided with a detecting pole end and a pole circuit end which are communicated with each other, and the enzyme reaction zone is connected to the front end of the detecting pole end. Accordingly the planar biological detection test piece has the advantages of economical benefits in mass production and cost reduction, and further improved stability of product quality and market competitiveness.

Description

The plane formula biological detecting test piece
Technical field
The utility model is relevant for a kind of plane formula biological detecting test piece, especially refer to a kind of test piece of detection of biological liquid physiological situation,, know clearly it as the blood sugar in the human body blood, cholesterol or urine etc., its finished product has preferable stability, accuracy, and has economic benefit in preparation.
Background technology
Modern's life-span generally prolongs flourishing closely bound up with modern medical service science and technology, and many symptoms of hiding can be confirmed by the check in advance of medical equipment, also therefore reach the effect with early prevention and treatment.Just the blood sugar with human body is example, earlier by carrying out trace blood on the human body, react in the blood sugar ferment district that more described blood is splashed in the reaction test piece, then by the blood-sugar detecting instrument analysis, detected the blood glucose value of human body, make the user can handle first unusual symptom problem of blood glucose value early, avoid causing the relevant major disease that worsens.
Existing blood glucose response test piece such as Fig. 1, shown in Figure 2, described reaction test piece 90 is to be substrate 100 with single sheet type plastics film (as Polyimide), again with the graphite material of tool electric conductivity with wire mark carbon ink mode or in described base material 100 surperficial coating Copper Foils by little shadow (Photolithography), etching (Etching) mode forms the conduction road 93 that is conducted, described conduction road 93 includes the detecting electrode end 931 and the back segment electrode wires terminal 932 of leading portion, and be connected with a ferment reaction zone 92 (being provided with ferment) and siphon channel 91 in regular turn in the front end of described detecting electrode end 931, then cover one and go up epiphragma 96, expose described electrode wires terminal 932, cut into suitable size at last.
During use, contact the blood of being taked in described siphon channel 91, blood is drawn to ferment reaction zone 92 through capillarity, and glucose and ferment in the blood are reacted and the generation electron stream, electron stream by described detecting electrode end 931 to described electrode wires terminal 932, described electrode wires terminal 932 connects a blood-sugar detecting instrument 95, described blood-sugar detecting instrument 95 is measured electric current by electrode wires terminal 932, and convert thereof into an electric signal, more described electrical signal conversion is become a corresponding blood glucose concentration value at last.
Though aforementioned described existing blood glucose response test piece 90 can reach the effect of measuring blood value, still has many deficiencies on its method for making, as:
1. it is that the plastics film 100 with single sheet type is a base material, on described base material, form leading portion detecting electrode end 931 and back segment electrode wires terminal 932 again in wire mark carbon ink mode, because the length of described detecting electrode end 931 and electrode wires terminal 932 is long and surface area is bigger, therefore it is unstable to add man-hour, causes the uneven phenomenon of resistance value easily;
2. for reducing above-mentioned instability condition, be added with conductive silver printing ink of seal at present mostly in the carbon ink layer substrate, though can improve its stability like this, because silver-colored ink material cost is higher, and THICKNESS CONTROL requirement, drying process complexity also have many deficiencies during printing;
Above-mentioned adverse condition is to know altogether at present on the market to see.
Again, as the 7th, 465, No. 597 patent cases of United States Patent (USP) notification number, it is a kind of conduction road that makes the blood glucose response test piece with " photolithography mode "; In brief, its be on base material with photolithography, the conduction road graphic making that accurately will design becomes light shield (photo mask) and copies on the base material, and the principle of applied optics imaging, with graphic projection to base material.Because the light that light source sends has only the part through the light shield transparent region can continue to pass through, and is imaged on substrate surface; And substrate surface needs in advance smear photoresist (photo resist) again through cleaning, and like this, the light by light shield can produce reaction with photoresist, and we claim this step for exposing usually; Base material after the exposure needs through (development) step of developing, then the gaseous metal is deposited on default conduction road that does not have photoresist and the shielding layer that photoresist is arranged in the evaporation metal mode, photoresist as described in removing with chemical mode (as dissolving, acid etching) again, also can and then lift off and cover top metal, the metallic circuit that stays just forms the conduction road of wanting; Get off again, then carry out down-stream, as ferment reaction zone, loam cake are set and cut to finished product of suitable size etc.
Certainly, above-mentioned method of making blood glucose response test piece conduction road with " photolithography mode ", though its do not conduct electricity road precision not high and because of the surface area of carbon ink material bigger, impedance value control is difficult for problem, but because it is applied to the industry of highly accurate and accurate micro electronmechanical and circuit board wiring, so its production cost is also high comparatively speaking; On the other hand, it is with acid etching, removes the photoresist part with chemicals again, therefore has acid remnant and destroys enzyme activity, adds preparation control when not good, will cause ferment to be degenerated and environmental pollution problems, also shows nonideal preparation method.Therefore, how to improve the not enough problem of existing blood glucose response test piece method for making, should be this area and should make great efforts the important directions that solves, overcome.
Therefore, this creator is not because the deficiency of existing plane formula biological detecting test piece is attained the desirable fact, this case creator promptly sets about researching and developing its solution, hope can be developed a kind of plane formula biological detecting test piece that has more accuracy, stability and economy, popular and promote the development of this industry with service society, satisfy the generation that this creation is arranged through for a long time design.
The utility model content
The utility model purpose is providing a kind of plane formula biological detecting test piece, it is slide glass (material of described slide glass is for not having expansion performance) with the plastic sheeting, and finished in the mode of printing element object, make the economic benefit that has quick production, reduces cost, and can further simplify job sequence, and then promote the degree of stability and the market competitiveness of product quality.
For reaching described purpose, the utility model provides a kind of plane formula biological detecting test piece, it includes at least: a slide glass, it is made of plastic sheeting, be printed with the occlusion image plating on the described slide glass, and on described occlusion image plating, forming the conduction road with the evaporation metal conducting objects, described conduction road includes detecting electrode end and the electrode wires terminal that is conducted; A ferment reaction zone, it is arranged in described detecting electrode end front end.
For reaching described purpose, the utility model provides a kind of plane formula biological detecting test piece, and it includes at least: a slide glass, and it is that plastic sheeting constitutes; A conduction road, it is located on the described slide glass, and described conduction road further includes: the detecting electrode end, it is with coating or printing conductive material means and forming; And the electrode wires terminal, itself and described detecting electrode end are conducted, and described electrode wires terminal is after finishing described detecting electrode end, printing occlusion image plating on described slide glass, and on described occlusion image plating, form with the evaporation metal conducting objects; And a ferment reaction zone, be before being arranged in described detecting electrode end.
Wherein, be the coupling electrode that forms levels with the metallic conduction thing of evaporation in a end on the detecting electrode end away from described ferment reaction zone.
Wherein, the conductive material that forms described detecting electrode end is carbon ink or conductive silver printing ink.
Wherein, described slide glass is made of single or multiple lift plastic sheeting applying institute.
Wherein, described slide glass is constituted by PET film, PC film, PTFE film, anti-grand film, poly-carbonic acid resin film, plasticon, styrene film, vinyl cyanide film or butadiene copolymer film.
Wherein, the evaporation metal conductive material that forms described conduction road is: gold, silver, copper or aluminum metal.
The beneficial effects of the utility model:
It is the plane formula biological detecting test piece of demand with the environmental protection that the utility model provides a kind of, it handles occlusion image plating (as with non-toxic water printing ink) with mode of printing, and be covered with the layer of metal conductive coating by evaporation process comprehensively, remove the conductive coating block that is covered with the occlusion image plating with physics mode (as water-washing process) again, and show without the conduction road of covering; Because whole process can reach the environmental protection demand of preserving our planet for the physical characteristics preparation, and its control group value is easy, stable conductivity, has more little current detecting accuracy and the sensitivity of lift pins to the biochemical reaction of electricity.
The technological means that the utility model adopted comprises: a slide glass, and it is that plastic sheeting constitutes, and described slide glass is provided with the conduction road that forms with evaporation process, and the front and rear sections on described conduction road is provided with detecting electrode end and the electrode wires terminal that is conducted in regular turn; A ferment reaction zone is the front end that is arranged in described detecting electrode end; A last epiphragma be to be covered on the described slide glass, and epiphragma is to cover on the described slide glass on described, and exposes described electrode wires terminal, detects in order to facility.
Another technological means that the utility model adopted comprises: the detecting electrode end on conduction road is finished with coating or mode of printing with difficult conductive material (as carbon ink, conductive silver printing ink etc.) with ferment generation chemical change earlier on described slide glass, the evaporation metal conducting objects is to be covered in described electrode wires terminal and away from the described detecting electrode end of described ferment reaction zone again, conduct so that described electrode wires terminal and detecting electrode end are, and make the coupling electrode that forms levels away from the described detecting electrode end of described ferment reaction zone; Because the described detecting electrode end that contacts with described ferment reaction zone is for being difficult for producing with ferment the conductive material of chemical change, so the degree of stability of its detection is higher, be not easy and can change because of environment change or with the lengthening of pot life, and its change to lead the control of coefficient (CV) also easy.
Another technological means that the utility model adopted: described slide glass is that the plastic sheeting by a sheet is constituted, and is finished in the mode of a large amount of printing element objects again.
The plane formula biological detecting test piece that the utility model adopted further comprises: described slide glass is constituted by the plastic sheeting of a whole bundle drum, finished by the continuous mode of printing element objects in a large number of volume to volume (Roll to Roll) again, make and have the economic benefit of producing fast, reducing cost in a large number, and can further simplify job sequence, and then promote the degree of stability and the market competitiveness of product quality.
Description of drawings
Fig. 1 is the structural representation of existing blood glucose response test piece.
Fig. 2 is the preparation synoptic diagram of existing blood glucose response test piece.
Fig. 3 A-3D is the formation synoptic diagram of first kind of embodiment of the utility model.
Fig. 4 is the preparation flow figure of first kind of embodiment of the utility model.
Fig. 5 is the preparation flow synoptic diagram of first kind of embodiment of the utility model.
Fig. 6 is that synoptic diagram is arranged in the preparation of first kind of embodiment of the utility model.
Fig. 7 A-7E is the formation synoptic diagram of second kind of embodiment of the utility model.
Fig. 8 is the preparation flow figure of second kind of embodiment of the utility model.
[figure number is to as directed]
Biological detecting test piece 10A, 10C slide glass 11A, 11C
Occlusion image plating 12A, 12C conduct electricity road 13A, 13C
Detecting electrode end 131A, 131C electrode wires terminal 132A, 132C
Ferment reaction zone 16A, 16C
Last epiphragma 15A, 15C conductive coating 17A, 17C
Plastic sheeting 31 intaglio process wheel 32
Mesh plating 321 drying plants 33
Vacuum evaporation plating machine 34 washing facilitys 35
Ferment printing equipment 36 guillotines 37
Printing in advance or coating form detecting electrode (20C) on the plastic sheeting
Construction has the support plate (21A, 21C) of occlusion image plating
Evaporation forms conductive coating (22A, 22C)
Physics mode is removed the conductive coating block that is covered with the occlusion image plating, shows conduction road (23A, 23C)
Put ferment in the ferment reaction zone (24A, 24C) of support plate
Epiphragma is in support plate (25A, 25C) in the applying
Cut into unit object finished product (26A, 26C)
Embodiment
For to technology of the present utility model, feature and the effect that is reached more have further understanding and understanding, sincerely help with preferred embodiment figure and cooperate detailed explanation, illustrate as the back: see also Fig. 3 C, Fig. 3 D, first kind of embodiment that the utility model plane formula biological detecting test piece constitutes, described biological detecting test piece 10A includes a slide glass 11A, described slide glass 11A is that continuous plastic sheeting for the sheet of single or multiple lift or whole bundle drum (is with whole bundle drum in present embodiment) is (as the PET film, the PC film, the PTFE film, anti-grand film (Nylon), poly-carbonic acid resin film (polycarbonate), plasticon (Polystyrenes), the styrene film, vinyl cyanide film or butadiene copolymer film ABS (Acrylonitrile butadiene styrene) etc. are not had a material of expansion performance, just be familiar with described operator, still can other material replace) constitute, and described slide glass 11A is provided with the conduction road 13A that forms with evaporation process, before the described conduction road 13A, the rear end is provided with detecting electrode end 131A and the electrode wires terminal 132A that is conducted in regular turn, described detecting electrode end 131A front end also is connected with a ferment reaction zone 16A, be provided with ferment in the described ferment reaction zone 16A, described slide glass 11A goes up and is provided with one and goes up epiphragma 15A, and described electrode wires terminal 132A is exposed, detect with facility.
See also shown in Fig. 3 A, Fig. 3 B, the formation of described plane formula biological detecting test piece 10A is to go up a printing occlusion image plating (Shadow Mask) 12A prior to described slide glass 11A, described occlusion image plating 12A manifests a conduction road 13A plating (is to carve mode with the moon in present embodiment, promptly be not coated with or print) on described detecting electrode end plating block and electrode wires terminal plating block; Continue after evaporation process, in order to form a conductive coating 17A, the conductive coating 17A block that will be covered with described occlusion image plating 12A is again removed with physics mode (as cleaning with water), and shows described detecting electrode end 131A and electrode wires terminal 132A; In better embodiment, described occlusion image plating 12A is by adopting non-toxic water printing ink to be formed by mode of printing.
See also Fig. 4 to Fig. 6, based on the formation of above-mentioned plane formula biological detecting test piece 10A, preparation of the present utility model comprises:
(1) with the continuously a large amount of described occlusion image plating of mode of printing construction 12A (21A) of sheet or whole bundle drum (is to be example with volume to volume Roll to Roll in present embodiment) (described mode of printing such as screen painting, or in the intaglio plate roller printing of present embodiment); Promptly get and be wound into the whole plastic sheeting 31 of drum of tying (as the PET film, the PC film, the PTFE film, anti-grand film, poly-carbonic acid resin film, plasticon, the styrene film, vinyl cyanide film or butadiene copolymer film etc. are not had a material of expansion performance) as the material of described slide glass 11A (as shown in Figure 3A), described slide glass 11A is that the intaglio plate through intaglio process wheel 32 prints (or screen painting continuously, just being familiar with described operator still can other mode of printing implements), on the described intaglio process wheel 32 is to be provided with a plurality of mesh platings 321 that are installed with non-toxic water printing ink, and described slide glass 11A is stamped have a layer occlusion image plating 12A.
(2) evaporation forms conductive coating (22A); In the described described slide glass 11A of layer occlusion image plating 12A that is printed with behind the drying procedure of a drying plant 33, enter the evaporation process of a vacuum evaporation plating machine 34, described evaporation process is that described slide glass 11A is passed the evaporation roller and is wound on (not icon) on the rolling station, continue and vacuumize with vacuum pump, the heating evaporation source makes highly purified conductive material (as gold, silver, copper, metals such as aluminium) at high temperature melt and flash to gaseous state, continue and activate the winding film system, after the film travelling speed reaches certain numerical value, open the described slide glass 11A surface deposition that baffle plate is moving the gaseous state electrically conductive particles, cooling is promptly gone up in described slide glass 11A and is formed the continuous conductive coating 17A of one deck.
In this evaporation process, evaporation quick-action that can be by control conductive material (as metals such as gold, silver, copper, aluminium), as described in vacuum tightness etc. in the translational speed of slide glass 11A and the deposited chamber decide the thickness of control evaporation conductive coating 17A, make the preparation quality of described detecting electrode end 131A and electrode wires terminal 132A be able to accurate control.
(3) remove the conductive coating 17A (23A) that is covered with occlusion image plating 12A; The described slide glass 11A that promptly finishes evaporation process is through a washing facility 35 (as washing), the conductive coating 17A block washing that will be covered with described occlusion image plating 12A (Mask) is removed, and its part of exposing is described detecting electrode end 131A and electrode wires terminal 132A.
(4) put ferment in the ferment reaction zone (24A) of support plate; Be that described slide glass 11A continues and carries out the setting of ferment through a ferment printing equipment 36, ferment be located at described slide glass 11A go up (ferment reaction zone 16A) that the setting of described ferment can be mode operations such as intaglio plate, wire mark, spray printing printing or drop.
Moreover if the utility model is with intaglio printing, and then its ferment thickness can be set according to intaglio plate degree of depth difference, and it is more accurate to make that the effect of its ferment reaction is better, detection is able to.
(5) in the applying epiphragma 15A in slide glass 11A (25A); Promptly with continuous printing colloid material (as glue, acryl glue) in as described in slide glass 11A go up and fit relatively as described in go up epiphragma 15A, and described electrode wires terminal 132A is exposed, detect in order to facility.
What deserves to be mentioned is, the ferment reaction zone 16A of described plastic sheeting 31 (detecting electrode end 131A) is two relative settings of facing, as shown in Figure 6, so once go up the applying of epiphragma 15A and can finish the setting of a plurality of epiphragma 15A of going up of two row simultaneously, and more promote its preparation efficiency.
(6) cut into unit object finished product (26A); Be about to describedly the relevant plastic sheeting 31 that constitutes has been set enters a guillotine 37 and carry out cutting program, and complete a plurality of plane formula biological detecting test piece 10A (blood sugar test device) finished product.
See also Fig. 7 A to Fig. 7 E again, it is second kind of embodiment of the utility model plane formula biological detecting test piece, described biological detecting test piece 10C includes a slide glass 11C (formation of described slide glass is identical with first kind of embodiment), and described slide glass 11C is provided with conduction road 13C, and described conduction road 13C includes detecting electrode end 131C and electrode wires terminal 132C; Wherein, described detecting electrode end 131C finishes with coating or mode of printing with difficult conductive material (as carbon ink, conductive silver printing ink etc.) with ferment generation chemical change earlier; As described in evaporation metal conducting objects (as metals such as gold, silver, copper, aluminium) is covered in again electrode wires terminal 132C and away from as described in ferment reaction zone 16C as described in detecting electrode end 131C, conduct so that described electrode wires terminal 132C and described detecting electrode end 131C are, and make described detecting electrode end 131C form the coupling electrode of levels; Because the described detecting electrode end 131C that contacts with described ferment reaction zone 16C is the conductive material for stability, can not produce chemical change with the ferment of described ferment reaction zone 16C, so the degree of stability of its detection is higher, be not easy and can change because of environment change or with the lengthening of pot life, and its change to lead the control of coefficient (CV) also easy.
See also Fig. 8, it is based on the preparation of above-mentioned second kind of embodiment, and it comprises:
(1) printing in advance or coating form detecting electrode (20C) on the plastic sheeting: promptly get sheet or be wound into the material of the plastic sheeting of whole bundle drum as described slide glass, and printing or coating are difficult for producing with ferment the conductive material of chemical change on described plastic sheeting, to form the detecting electrode end;
(2) has the support plate (21C) of occlusion image plating with the mode of printing construction; Promptly stamp in described slide glass 11C and have one deck occlusion image plating 12C, described occlusion image plating also is connected with described detecting electrode end 131C;
(3) evaporation forms conductive coating (22C); In described be printed with the described slide glass 11C drying program of occlusion image plating 12C after, enter an evaporation process, be covered in described electrode wires terminal 12C and away from the described detecting electrode end 131C of described ferment reaction zone 16C, conduct so that described electrode wires terminal 132C and detecting electrode end 131C are, and make described detecting electrode end 131C form the coupling electrode of levels;
(4) remove the conductive coating (23C) that removes to be covered with occlusion image plating 12C; The described slide glass 11C that promptly finishes evaporation process is through a washing facility, the conductive coating 17C block washing of occlusion image plating 12C (Mask) is removed as described in will being covered with physics mode (as washing), and its part of exposing is described detecting electrode end 131C and electrode wires terminal 132C.
(5) put ferment in the ferment reaction zone (24C) of support plate; Be that described slide glass 11C continues and carries out the setting of ferment through a ferment printing equipment 36, make described ferment be placed in described slide glass 11C and go up (ferment reaction zone) that being provided with of described ferment can be mode operations such as intaglio plate, wire mark, spray printing printing or drop.
(6) in the applying epiphragma 15C in slide glass 11C (25C); Promptly with continuous printing colloid material (as glue, acryl glue) in as described in slide glass 11C go up and fit relatively as described in go up epiphragma 15C, and described electrode wires terminal 132C is exposed, detect in order to facility.
(7) cut into unit object finished product (26C); Be about to describedly the relevant plastic sheeting 31 that constitutes has been set enters a guillotine and carry out cutting program, and complete a plurality of plane formula biological detecting test piece 10C (blood sugar test device) finished product.
The utility model makes the plane formula biological detecting test piece form conductive characteristic by physics mode by above-mentioned formation, can reach the environmental protection demand of preserving our planet, and impedance Control is easy, stable conductivity, and then has splendid physiological measurements accuracy and sensitivity.
In sum, the utility model has really met the requirement of novel patent, proposes patented claim in accordance with the law.Just the above only is the utility model preferred embodiment, be not to be used for limiting the scope that the utility model is implemented, so every equalization of doing according to the described shape of the utility model claim, structure, feature and spirit changes and modifies, and all should be included in the claim of the present utility model.

Claims (7)

1. a plane formula biological detecting test piece is characterized in that, includes at least:
A slide glass, it is made of plastic sheeting, is printed with the occlusion image plating on the described slide glass, and forms the conduction road with the evaporation metal conducting objects on described occlusion image plating, and described conduction road includes detecting electrode end and the electrode wires terminal that is conducted;
A ferment reaction zone is before it is arranged in described detecting electrode end.
2. a plane formula biological detecting test piece is characterized in that, includes at least:
One slide glass, it is that plastic sheeting constitutes;
A conduction road, it is located on the described slide glass, and described conduction road further includes:
The detecting electrode end, it is with coating or printing conductive material means and forming; And
The electrode wires terminal, itself and described detecting electrode end are conducted, and described electrode wires terminal is after finishing described detecting electrode end, printing occlusion image plating on described slide glass, and on described occlusion image plating, form with the evaporation metal conducting objects; And
A ferment reaction zone is to be arranged in described detecting electrode end front end.
3. plane formula biological detecting test piece as claimed in claim 2 is characterized in that, is the coupling electrode that forms levels with the metallic conduction thing of evaporation in the end away from described ferment reaction zone on the detecting electrode end.
4. plane formula biological detecting test piece as claimed in claim 2 is characterized in that, the conductive material that forms described detecting electrode end is carbon ink or conductive silver printing ink.
5. plane formula biological detecting test piece as claimed in claim 1 or 2 is characterized in that, described slide glass is made of single or multiple lift plastic sheeting applying institute.
6. plane formula biological detecting test piece as claimed in claim 1 or 2, it is characterized in that described slide glass is constituted by PET film, PC film, PTFE film, anti-grand film, poly-carbonic acid resin film, plasticon, styrene film, vinyl cyanide film or butadiene copolymer film.
7. plane formula biological detecting test piece as claimed in claim 1 or 2 is characterized in that, the evaporation metal conductive material that forms described conduction road is: gold, silver, copper or aluminum metal.
CN2010201292536U 2010-03-02 2010-03-02 Planar biological detection test piece Expired - Fee Related CN201607421U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102192937A (en) * 2010-03-02 2011-09-21 翰沃生电科技股份有限公司 Method for preparing planar bioinstrumentation test block and finished product of planar bioinstrumentation test block
CN110715965A (en) * 2019-09-09 2020-01-21 浙江大学 Preparation method of double-sided physiological value test piece and product

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102192937A (en) * 2010-03-02 2011-09-21 翰沃生电科技股份有限公司 Method for preparing planar bioinstrumentation test block and finished product of planar bioinstrumentation test block
CN110715965A (en) * 2019-09-09 2020-01-21 浙江大学 Preparation method of double-sided physiological value test piece and product

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Granted publication date: 20101013

Termination date: 20180302