CN201489184U - Line structure optical confocal scanning microscope - Google Patents
Line structure optical confocal scanning microscope Download PDFInfo
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- CN201489184U CN201489184U CN2009201410597U CN200920141059U CN201489184U CN 201489184 U CN201489184 U CN 201489184U CN 2009201410597 U CN2009201410597 U CN 2009201410597U CN 200920141059 U CN200920141059 U CN 200920141059U CN 201489184 U CN201489184 U CN 201489184U
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- finedraw
- object lens
- spectroscope
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- condenser
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Abstract
The utility model discloses a line structure optical confocal scanning microscope, which comprises an image acquisition system, a beeline scanning system and a main supporting frame. The difference with the prior art is that an optical imaging system is arranged between the image acquisition system and the beeline scanning system and is arranged on the main supporting frame, a beam splitter thereof corresponds to a CCD in the image acquisition system and an objective lens is vertically arranged above an objective table in the beeline scanning system. A tested finedraw surface, a finedraw light source and finedraw detection are conjugate in the optical imaging system. The utility model has the advantages of novel and simple structure, low cost and price and quick imaging speed.
Description
Technical field
The utility model relates to microscope, particularly the line-structured light confocal scan microscope.
Background technology
Nineteen fifty-seven, the post-doctor Minsky of Harvard University proposed the notion of confocal microscopy first.Confocal microscopy is compared different being with common microscopy, adopts the spot light object in the confocal microscopy, with object lens with point-sourcre imaging in object a bit on, the light by this point reflection is received by point probe more then.When measured surface, pointolite and the point probe three conjugation, reach confocal effect, it is that lateral resolution or longitudinal frame all are common microscopy resolution
Doubly.Although confocal microscopy can obtain higher resolution, two weak points are arranged:
1. need pointwise to scan during because of its imaging, and need carry out X, the scanning of Y diaxon during to planar imaging, image taking speed is slow;
2. because of it is that pointolite participates in imaging, its luminous energy seldom so when gathering image-forming information, need to use the photoelectricity booster that the collection light spot signal is strengthened, causes the cost made too high like this.
Because of above-mentioned shortcoming, except that some image quality require high microscope, other microscope is seldom used, and domestic except that the microscope of only a few business research production based on the confocal microscopy principle, and remaining domestic confocal microscope all is dependence on import basically.
The utility model content
2005, the researchist of optical, mechanical and electronic integration research institute of Guilin Electronic Science and Technology Univ. is theoretical foundation with the Fourier optics, with the Fourier optics of thin lens and three-dimensional point spread function as starting point, research point confocal microscopy image-forming principle, line-structured light confocal microscopic imaging mechanism is explored, and taken the lead in proposing at home the theory of line-structured light cofocus scanning microscopy.
The purpose of this utility model is at the deficiencies in the prior art, and provide a kind of novel structure, simple, cost is lower, low price, image taking speed are very fast, based on the line-structured light confocal scan microscope of line-structured light cofocus scanning microscopy.
The purpose of this utility model realizes by following technical proposals:
The line-structured light confocal scan microscope, comprise image capturing system, the rectilinear scanning system, main backstop, control motor Single Chip Microcomputer (SCM) system and image reconstruction software systems, the image reconstruction software systems are installed in the PC, image capturing system is connected with the image reconstruction software systems by PC, the rectilinear scanning system, control motor Single Chip Microcomputer (SCM) system and PC are linked in sequence, unlike the prior art be: between image capturing system and rectilinear scanning system, be provided with optical imaging system, this optical imaging system is tested finedraw surface, the optical imaging system of finedraw light source and detection finedraw three conjugation, it is arranged on the main backstop, its spectroscope is corresponding with the CCD in the image capturing system, and object lens are vertically set on the objective table top in the rectilinear scanning system.
Described optical imaging system is by focusing handwheel, object lens, the focusing bracing frame, attachment objective, light source, group before the condenser, the finedraw diaphragm, organize behind the condenser, spectroscope, diaphragm holder and spectroscope holder are formed, spectroscope is arranged on the spectroscope holder, the finedraw diaphragm is arranged in the diaphragm holder, the spectroscope holder, organize behind the condenser, the diaphragm holder, group is linked in sequence with light source before the condenser, and be arranged on the main backstop upper surface, object lens are arranged on the focusing supporting framing front end, focusing bracing frame rear end is connected with the focusing handwheel that is arranged on the main backstop middle part by gear set, attachment objective is arranged on the main backstop front end, between object lens and spectroscope.
The optical centre of described object lens, attachment objective and CCD is on same axis, group and the optical centre of light source be on same axis before group behind the described condenser, finedraw diaphragm, the condenser, and light source is vertical mutually with attachment objective place axis with object lens with finedraw diaphragm place axis.
Described object lens are the infinite distance object lens.
Formation finedraw light source behind the finedraw diaphragm of before horizontal light that light source sends is respectively by condenser, organizing behind group and the condenser, level is organized behind condenser then, the place is reflected vertically downward at spectroscope, vertical through behind attachment objective and the infinite distance object lens, picture with the finedraw light source, be imaged on the object plane, form tested finedraw surface, the light (the two kinds of optical informations of picture that comprise object plane and finedraw light source in the light) that then has tested finedraw surface information reflects vertically upward through object plane together, respectively by the infinite distance object lens, attachment objective, picture with measured surface behind the spectroscope is imaged on the CCD target surface, the picture light that wherein has the finedraw light source forms surveys finedraw, and the light that has object plane information forms the picture of actual tested object plane, and what in fact survey finedraw and tested object plane similarly is to coincide together.
By above-mentioned explanation as can be seen: the light that light source sends is surveyed finedraw at the picture that forms tested finedraw surface, finedraw light source on the object plane through forming behind the optical imaging system through formation finedraw light source, finedraw light source behind the finedraw diaphragm on the CCD target surface, obviously this moment, this three was the conjugate relation that belongs to imaging, promptly formed the line-structured light confocal microscope system.
Scanning imagery mode of the present utility model is described as follows:
After opening this microscope, adjust the telescope to one's eyes by focusing handwheel and to focus, after treating that focusing finishes, start image reconstruction software, PC sends sweep signal for automatically control motor Single Chip Microcomputer (SCM) system (hereinafter to be referred as single-chip microcomputer), the stepper motor of Single-chip Controlling rectilinear scanning system rotates, the screw mandrel that is connected on the motor by drive rotates, thereby the control objective table moves forward, single-chip microcomputer is subjected to the internal processes timing controlled, timing stops operating to the Single-chip Controlling stepper motor, and send the signal that puts in place to PC, PC control image capturing system begins to gather the finedraw image, after collection finishes, PC sends to single-chip microcomputer and gathers the signal that finishes, the Single-chip Controlling stepper motor is rotated further, thereby objective table continues to reach, timing to single-chip microcomputer once more the control step motor stop operating, and send the signal that puts in place to PC again, PC controls once more image capturing system and begins to gather the finedraw image, after collection finishes, PC sends to single-chip microcomputer once more and gathers the signal that finishes, big or small needs according to imaging, behind the repetition said process tens times, these tens images that the image reconstruction software systems will be gathered carry out the reconstruct of image, form a full picture after the reconstruct, and this picture promptly is the image of required tested object plane.
Although the utility model only lateral resolution can reach or (promptly be common microscopy lateral resolution near the level of conventional point confocal microscopy
Doubly), but than the high imaging system of common microscopy, very high using value and cost performance are arranged still for some imaging requirements.
The utility model has the advantages that:
1, only just realized the line confocal microscope system with group and infinite distance object lens microscopic system behind group, the condenser before the finedraw diaphragm cooperation condenser, simple in structure;
2, having used line-structured light cofocus scanning microscopy theory because of it makes it simplify scanning mechanism and use low-light (level), thereby the CCD images acquired replaces the photoelectricity booster to greatly reduce cost, this microscopical manufacturing cost is 1/4～1/6 of existing some confocal microscope approximately, and equipment cost is lower;
Only need one-dimensional scanning during 3, because of planar imaging, so adopt under the moving scan mode of thing, become identical when size of, its imaging time shortens 1/2 than the scanning imagery time of existing some confocal microscope, and image taking speed is very fast;
4, novel structure of the present invention, floor area is little, easily installation, easy to operate.
Description of drawings
Fig. 1 be the utility model optical imaging system structure and with the equipped synoptic diagram that concerns of image capturing system and rectilinear scanning system;
Fig. 2 is the utility model block diagram.
Among the figure, 1. group 13. spectroscopes 14. connect cover 15. and connect cover bracing frame 16.CCD 17. diaphragm holders 18. spectroscope holders 19. screw mandrel fixed mounts behind the preceding group of main backstop 2. focusing handwheels 3. stepper motors, 4. screw mandrels, 5. objective tables, 6. object lens, 7. focusing bracing frames, 8. attachment objectives, 9. light sources, 10. condensers 11. finedraw diaphragms 12. condensers
Embodiment
Below in conjunction with drawings and Examples the utility model content being made further indefiniteness sets forth.
Embodiment:
With reference to Fig. 1 Fig. 2, the utility model line-structured light confocal scan microscope, comprise image capturing system, the rectilinear scanning system, main backstop 1, control motor Single Chip Microcomputer (SCM) system and image reconstruction software systems, the image reconstruction software systems are installed in the PC, image capturing system is connected with the image reconstruction software systems by PC, the rectilinear scanning system, control motor Single Chip Microcomputer (SCM) system and PC are linked in sequence, between image capturing system and rectilinear scanning system, be provided with optical imaging system, this optical imaging system is tested finedraw surface, the optical imaging system of finedraw light source and detection finedraw three conjugation, it is arranged on the main backstop 1, its spectroscope 13 is corresponding with the CCD16 in the image capturing system, and object lens 6 are vertically set on objective table 5 tops in the rectilinear scanning system.
Image capturing system comprises and connects cover 14, meets cover bracing frame 15 and CCD16, connects cover 14 and is arranged on and connects on the cover bracing frame 15, and its rear end is connected with CCD16.
The rectilinear scanning system comprises stepper motor 3, screw mandrel 4, objective table 5 and screw mandrel fixed mount 19, and objective table 5 is equipped with screw mandrel 4, and screw mandrel 4 two ends are enclosed within on the screw mandrel fixed mount 19, and an end is connected with motor 3.
Optical imaging system is by focusing handwheel 2, object lens 6, focusing bracing frame 7, attachment objective 8, light source 9, group 10 before the condenser, finedraw diaphragm 11, organize 12 behind the condenser, spectroscope 13, diaphragm holder 17 and spectroscope holder 18 are formed, spectroscope 13 is arranged on the spectroscope holder 18, finedraw diaphragm 11 is arranged in the diaphragm holder 17, spectroscope holder 18, organize 12 behind the condenser, diaphragm holder 17, group 10 is linked in sequence with light source 9 before the condenser, and be arranged on main backstop 1 upper surface, object lens 6 are arranged on focusing bracing frame 7 front ends, focusing bracing frame 7 rear ends are connected with the focusing handwheel 2 that is arranged on main backstop 1 middle part by gear set, attachment objective 8 is arranged on main backstop 1 front end, between object lens 6 and spectroscope 13.
The optical centre of object lens 6, attachment objective 8 and CCD16 is on same axis, organize 12 behind the condenser, group 10 and the optical centre of light source 9 are on same axis before the finedraw diaphragm 11, condenser, and light source 9 is vertical mutually with attachment objective 8 place axis with object lens 6 with finedraw diaphragm 11 place axis.
The light that light source sends is surveyed finedraw at the picture that forms tested finedraw surface, finedraw light source on the object plane through forming behind the optical imaging system through formation finedraw light source, finedraw light source behind the finedraw diaphragm 11 on the CCD16 target surface, obviously this moment, this three was the conjugate relation that belongs to imaging, promptly formed the line-structured light confocal microscope system.
Claims (4)
1. line-structured light confocal scan microscope, comprise image capturing system, rectilinear scanning system and main backstop (1), it is characterized in that: between image capturing system and rectilinear scanning system, be provided with optical imaging system, this optical imaging system is arranged on the main backstop (1), its spectroscope (13) is corresponding with the CCD (16) in the image capturing system, and object lens (6) are vertically set on objective table (5) top in the rectilinear scanning system.
2. line-structured light confocal scan microscope according to claim 1, it is characterized in that: described optical imaging system is by focusing handwheel (2), object lens (6), focusing bracing frame (7), attachment objective (8), light source (9), group (10) before the condenser, finedraw diaphragm (11), group (12) behind the condenser, spectroscope (13), diaphragm holder (17) and spectroscope holder (18) are formed, spectroscope (13) is arranged on the spectroscope holder (18), finedraw diaphragm (11) is arranged in the diaphragm holder (17), spectroscope holder (18), group (12) behind the condenser, diaphragm holder (17), group (10) is linked in sequence with light source (9) before the condenser, and be arranged on main backstop (1) upper surface, object lens (6) are arranged on focusing bracing frame (7) front end, focusing bracing frame (7) rear end is connected with the focusing handwheel (2) that is arranged on main backstop (1) middle part by gear set, attachment objective (8) is arranged on main backstop (1) front end, is positioned between object lens (6) and the spectroscope (13).
3. line-structured light confocal scan microscope according to claim 1 and 2, it is characterized in that: the optical centre of described object lens (6), attachment objective (8) and CCD (16) is on same axis, the optical centre of organizing (10) and light source (9) before group (12), finedraw diaphragm (11), the condenser behind the described condenser is on same axis, and light source (9) is vertical mutually with attachment objective (8) place axis with object lens (6) with finedraw diaphragm (11) place axis.
4. line-structured light confocal scan microscope according to claim 1 and 2 is characterized in that: described object lens (6) are the infinite distance object lens.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201410597U CN201489184U (en) | 2009-07-11 | 2009-07-11 | Line structure optical confocal scanning microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201410597U CN201489184U (en) | 2009-07-11 | 2009-07-11 | Line structure optical confocal scanning microscope |
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|---|---|
| CN201489184U true CN201489184U (en) | 2010-05-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2009201410597U Expired - Fee Related CN201489184U (en) | 2009-07-11 | 2009-07-11 | Line structure optical confocal scanning microscope |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103323442A (en) * | 2013-06-20 | 2013-09-25 | 中国科学院苏州生物医学工程技术研究所 | LED (light emitted diode) line scanning optical system applied to confocal microscopy |
| CN103645136A (en) * | 2013-11-22 | 2014-03-19 | 深圳先进技术研究院 | Method and device for improving multiphoton fluorescence microscope imaging resolution |
| CN110568601A (en) * | 2019-08-30 | 2019-12-13 | 北京临近空间飞行器系统工程研究所 | Image scanning system |
-
2009
- 2009-07-11 CN CN2009201410597U patent/CN201489184U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103323442A (en) * | 2013-06-20 | 2013-09-25 | 中国科学院苏州生物医学工程技术研究所 | LED (light emitted diode) line scanning optical system applied to confocal microscopy |
| CN103645136A (en) * | 2013-11-22 | 2014-03-19 | 深圳先进技术研究院 | Method and device for improving multiphoton fluorescence microscope imaging resolution |
| CN110568601A (en) * | 2019-08-30 | 2019-12-13 | 北京临近空间飞行器系统工程研究所 | Image scanning system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100526 Termination date: 20110711 |